Genome Wide Studies Of Dna And Rna With Modifications Through High Throughput Sequencing Analysis

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Genome-wide Studies of DNA and RNA with Modifications Through High-throughput Sequencing Analysis

Author : Blythe S. Moreland
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (112 download)

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Book Synopsis Genome-wide Studies of DNA and RNA with Modifications Through High-throughput Sequencing Analysis by : Blythe S. Moreland

Download or read book Genome-wide Studies of DNA and RNA with Modifications Through High-throughput Sequencing Analysis written by Blythe S. Moreland and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA/RNA-protein interactions are crucial to the maintenance, processing, and regulation of the genetic information contained within a cell. Sets of particular DNA/RNA-protein interactions can confer specificity to a protein's binding behavior, which makes the consideration of DNA and RNA with modifications interesting in how they alter the landscape of interactions. High-throughput sequencing (HTS) techniques of DNA and RNA can offer unmatched genome-wide characterizations of where and how proteins interact with DNA and RNA by generating libraries of millions of reads that can capture the whole distribution of these interaction events. In this dissertation we used HTS analysis techniques to probe the location and state of two nucleic acid modifications on a genome-wide scale: The methylation of Cytosines in the CpG dinucleotide (mCpG) in the human genome, and the nucleotide addition by 3'-5' polymerases to the 5'-ends of RNAs. In the case of DNA methylation, the sequencing data represents DNA fragments enriched in mCpGs by way of interaction with the methyl-CpG-binding domain (MBD) protein MBD2. In the case of 5'-end nucleotide addition, the sequencing data is a snapshot of non-coding RNAs whose 5'-ends may have been extended by a Thg1-like protein (TLP). In both sets of studies, the information about the modification in turn conveys information on the relevant DNA-protein and RNA-protein interactions, and what sequences or structures are preferred by the proteins reading or depositing the modifications. In Chapter 2, a deep sample of methylated genomic DNA allows us to characterize the sequence, spacing, and mCpG density biases introduced through MBD2 interaction during a protein pulldown experiment. In Chapter 3, we use the characterizations of those interactions -- the 3 bp minimum separation between mCpGs, the nonlinear relationship between mCpG number and pulldown efficiency -- to build a model to predict expected pulldown from a SssI Control sample, which is then used to increase the accuracy of methylation quantification from MBD pulldown data. We find that the use of our pulldown alignment model of an SssI Control data set is suitable for substitution for observed SssI Control data with use of the empirical Bayes' model BayMeth. In Chapter 4, we pivot to developing a HTS screen for unknown substrates of TLPs, BtTLP and DdiTLP4, by detecting changes in 5'-end composition under different TLP conditions. Importantly, we detect BtTLP's activity on tRNA-His, of U-1 addition, which sufficiently allows for the tRNA's essential recognition by Histidyl-tRNA synthetase (HisRS). HTS techniques and analysis will continue to improve our ability to differentiate the distributions of DNA/RNA-protein interactions between "background" and "modified" states of nucleic acids.

Evolution of Translational Omics

Author : Institute of Medicine
Publisher : National Academies Press
ISBN 13 : 0309224187
Total Pages : 354 pages
Book Rating : 4.3/5 (92 download)

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Book Synopsis Evolution of Translational Omics by : Institute of Medicine

Download or read book Evolution of Translational Omics written by Institute of Medicine and published by National Academies Press. This book was released on 2012-09-13 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Technologies collectively called omics enable simultaneous measurement of an enormous number of biomolecules; for example, genomics investigates thousands of DNA sequences, and proteomics examines large numbers of proteins. Scientists are using these technologies to develop innovative tests to detect disease and to predict a patient's likelihood of responding to specific drugs. Following a recent case involving premature use of omics-based tests in cancer clinical trials at Duke University, the NCI requested that the IOM establish a committee to recommend ways to strengthen omics-based test development and evaluation. This report identifies best practices to enhance development, evaluation, and translation of omics-based tests while simultaneously reinforcing steps to ensure that these tests are appropriately assessed for scientific validity before they are used to guide patient treatment in clinical trials.

Advancing Development of Synthetic Gene Regulators

Author : Anandhakumar Chandran
Publisher : Springer
ISBN 13 : 9811065470
Total Pages : 123 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Advancing Development of Synthetic Gene Regulators by : Anandhakumar Chandran

Download or read book Advancing Development of Synthetic Gene Regulators written by Anandhakumar Chandran and published by Springer. This book was released on 2017-09-19 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.

New High Throughput Technologies for DNA Sequencing and Genomics

Author : Keith R. Mitchelson
Publisher : Elsevier
ISBN 13 : 0080471285
Total Pages : 399 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis New High Throughput Technologies for DNA Sequencing and Genomics by : Keith R. Mitchelson

Download or read book New High Throughput Technologies for DNA Sequencing and Genomics written by Keith R. Mitchelson and published by Elsevier. This book was released on 2011-09-22 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into today's multibillion dollar industry. This growth has spurred the development of new sequencing technologies that do not involve either electrophoresis or Sanger sequencing chemistries. Sequencing by Synthesis (SBS) involves multiple parallel micro-sequencing addition events occurring on a surface, where data from each round is detected by imaging. New High Throughput Technologies for DNA Sequencing and Genomics is the second volume in the Perspectives in Bioanalysis series, which looks at the electroanalytical chemistry of nucleic acids and proteins, development of electrochemical sensors and their application in biomedicine and in the new fields of genomics and proteomics. The authors have expertly formatted the information for a wide variety of readers, including new developments that will inspire students and young scientists to create new tools for science and medicine in the 21st century.Reviews of complementary developments in Sanger and SBS sequencing chemistries, capillary electrophoresis and microdevice integration, MS sequencing and applications set the framework for the book.* 'Hot Topic' with DNA sequencing continuing as a major research activity in many areas of life science and medicine.* Bringing together new developments in DNA sequencing technology* Reviewing issues relevant to the new applications used

Computational Genomics with R

Author : Altuna Akalin
Publisher : CRC Press
ISBN 13 : 1498781861
Total Pages : 463 pages
Book Rating : 4.4/5 (987 download)

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Book Synopsis Computational Genomics with R by : Altuna Akalin

Download or read book Computational Genomics with R written by Altuna Akalin and published by CRC Press. This book was released on 2020-12-16 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: Computational Genomics with R provides a starting point for beginners in genomic data analysis and also guides more advanced practitioners to sophisticated data analysis techniques in genomics. The book covers topics from R programming, to machine learning and statistics, to the latest genomic data analysis techniques. The text provides accessible information and explanations, always with the genomics context in the background. This also contains practical and well-documented examples in R so readers can analyze their data by simply reusing the code presented. As the field of computational genomics is interdisciplinary, it requires different starting points for people with different backgrounds. For example, a biologist might skip sections on basic genome biology and start with R programming, whereas a computer scientist might want to start with genome biology. After reading: You will have the basics of R and be able to dive right into specialized uses of R for computational genomics such as using Bioconductor packages. You will be familiar with statistics, supervised and unsupervised learning techniques that are important in data modeling, and exploratory analysis of high-dimensional data. You will understand genomic intervals and operations on them that are used for tasks such as aligned read counting and genomic feature annotation. You will know the basics of processing and quality checking high-throughput sequencing data. You will be able to do sequence analysis, such as calculating GC content for parts of a genome or finding transcription factor binding sites. You will know about visualization techniques used in genomics, such as heatmaps, meta-gene plots, and genomic track visualization. You will be familiar with analysis of different high-throughput sequencing data sets, such as RNA-seq, ChIP-seq, and BS-seq. You will know basic techniques for integrating and interpreting multi-omics datasets. Altuna Akalin is a group leader and head of the Bioinformatics and Omics Data Science Platform at the Berlin Institute of Medical Systems Biology, Max Delbrück Center, Berlin. He has been developing computational methods for analyzing and integrating large-scale genomics data sets since 2002. He has published an extensive body of work in this area. The framework for this book grew out of the yearly computational genomics courses he has been organizing and teaching since 2015.

Paleogenomics

Author : Charlotte Lindqvist
Publisher : Springer
ISBN 13 : 3030047539
Total Pages : 427 pages
Book Rating : 4.0/5 (3 download)

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Book Synopsis Paleogenomics by : Charlotte Lindqvist

Download or read book Paleogenomics written by Charlotte Lindqvist and published by Springer. This book was released on 2019-01-07 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in genome-scale DNA sequencing technologies have revolutionized genetic research on ancient organisms, extinct species, and past environments. When it is recoverable after hundreds or thousands of years of unintended preservation, “ancient DNA” (or aDNA) is often highly degraded, necessitating specialized handling and analytical approaches. Paleogenomics defines the field of reconstructing and analyzing the genomes of historic or long-dead organisms, most often through comparison with modern representatives of the same or similar species. The opportunity to isolate and study paleogenomes has radically transformed many fields, spanning biology, anthropology, agriculture, and medicine. Examples include understanding evolutionary relationships of extinct species known only from fossils, the domestication of plants and animals, and the evolution and geographical spread of certain pathogens. This pioneering book presents a snapshot view of the history, current status, and future prospects of paleogenomics, taking a broad viewpoint that covers a range of topics and organisms to provide an up-to-date status of the applications, challenges, and promise of the field. This book is intended for a variety of readerships, including upper-level undergraduate and graduate students, professionals and experts in the field, as well as anyone excited by the extraordinary insights that paleogenomics offers.

Methods in Yeast Genetics

Author : David C. Amberg
Publisher : CSHL Press
ISBN 13 : 0879697288
Total Pages : 250 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Methods in Yeast Genetics by : David C. Amberg

Download or read book Methods in Yeast Genetics written by David C. Amberg and published by CSHL Press. This book was released on 2005 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.

High-Density Sequencing Applications in Microbial Molecular Genetics

Author :
Publisher : Academic Press
ISBN 13 : 0128159944
Total Pages : 576 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis High-Density Sequencing Applications in Microbial Molecular Genetics by :

Download or read book High-Density Sequencing Applications in Microbial Molecular Genetics written by and published by Academic Press. This book was released on 2018-11-28 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-Density Sequencing Applications in Microbial Molecular Genetics, Volume 612 in the Methods of Enzymology series provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology. Methods permitting tens of millions of sequence reads in a single experiment have paved the way to genome-wide studies that are contributing to our understanding of the complexity of living systems. Chapters in this updated volume include Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq., Conformational studies of bacterial chromosomes by high-throughput sequencing methods, Measuring mRNA degradation, and more. Addition sections cover Global recognition patterns of bacterial RNA-binding proteins, High-resolution profiling of NMD targets, and the Generation of a metagenomic 3C/Hi-C library of human gut microbiota, Genome-wide mapping of yeast retrotransposons integration target sites, Measuring protein synthesis rates, Finding unsuspected partners of small RNAs with new screening approaches, Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output, RNA-based control of quorum sensing in Vibrio cholerae, amongst other highly regarded topics. Detail methods used in research articles that were recently published in leading journals Provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology

Molecular Diagnostics

Author : George P. Patrinos
Publisher : Academic Press
ISBN 13 : 0128029889
Total Pages : 526 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Molecular Diagnostics by : George P. Patrinos

Download or read book Molecular Diagnostics written by George P. Patrinos and published by Academic Press. This book was released on 2016-10-27 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. - Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories - Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression - Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field

RNA Abundance Analysis

Author : Hailing Jin
Publisher : Humana Press
ISBN 13 : 9781493959167
Total Pages : 248 pages
Book Rating : 4.9/5 (591 download)

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Book Synopsis RNA Abundance Analysis by : Hailing Jin

Download or read book RNA Abundance Analysis written by Hailing Jin and published by Humana Press. This book was released on 2016-05-01 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.

Modern Molecular Biology:

Author : Srinivasan Yegnasubramanian
Publisher : Springer Science & Business Media
ISBN 13 : 0387697454
Total Pages : 196 pages
Book Rating : 4.3/5 (876 download)

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Book Synopsis Modern Molecular Biology: by : Srinivasan Yegnasubramanian

Download or read book Modern Molecular Biology: written by Srinivasan Yegnasubramanian and published by Springer Science & Business Media. This book was released on 2010-09-02 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular biology has rapidly advanced since the discovery of the basic flow of information in life, from DNA to RNA to proteins. While there are several important and interesting exceptions to this general flow of information, the importance of these biological macromolecules in dictating the phenotypic nature of living creatures in health and disease is paramount. In the last one and a half decades, and particularly after the completion of the Human Genome Project, there has been an explosion of technologies that allow the broad characterization of these macromolecules in physiology, and the perturbations to these macromolecules that occur in diseases such as cancer. In this volume, we will explore the modern approaches used to characterize these macromolecules in an unbiased, systematic way. Such technologies are rapidly advancing our knowledge of the coordinated and complicated changes that occur during carcinogenesis, and are providing vital information that, when correctly interpreted by biostatistical/bioinformatics analyses, can be exploited for the prevention, diagnosis, and treatment of human cancers. The purpose of this volume is to provide an overview of modern molecular biological approaches to unbiased discovery in cancer research. Advances in molecular biology allowing unbiased analysis of changes in cancer initiation and progression will be overviewed. These include the strategies employed in modern genomics, gene expression analysis, and proteomics.

Epitranscriptomics: The Novel RNA Frontier

Author : Giovanni Nigita
Publisher : Frontiers Media SA
ISBN 13 : 2889457419
Total Pages : 127 pages
Book Rating : 4.8/5 (894 download)

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Book Synopsis Epitranscriptomics: The Novel RNA Frontier by : Giovanni Nigita

Download or read book Epitranscriptomics: The Novel RNA Frontier written by Giovanni Nigita and published by Frontiers Media SA. This book was released on 2019-01-31 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following the formulation of the central dogma of molecular biology and the later discovery of classes of non-coding RNAs, the primary focus of Genetics was essentially on variation of DNA aiming at elucidating biological pathways perturbed in diseases. Recently, extensive attention has shifted towards the study of posttranscriptional RNA modifications occurring in both protein-coding as well as non-coding RNAs, revealing a novel and finer layer of complexity in gene regulation. This, in turn, has led to the birth of the novel field of ‘Epitranscriptomics’. The recent increase of applications of high-throughput sequencing technology (HTS) has allowed the unprecedented opportunity to identify on a transcriptome-wide scale, millions of RNA modifications in human genes, counting today more than 140 distinct types such as: methylation (e.g. m6A, m1A, m5C, hm5C, 2’OMe) methylation (e.g. m6A, m1A, m5C, hm5C, 2’OMe), pseudourylation (?), deamination (e.g. A-to-I RNA editing). The scope of this Research Topic was to collect both reviews and research articles addressing the wet lab approaches and bioinformatics methodologies necessary to aid in the identification of novel RNA modifications and characterization of their biological functions. Among the articles embracing the aim of the Research Topic, we have collected four original research and methods articles, five reviews, and a technology article.

Computational Methods for Studying Gene Regulation and Genome Organization Using High-throughput DNA Sequencing

Author : Giancarlo A. Bonora
Publisher :
ISBN 13 :
Total Pages : 308 pages
Book Rating : 4.:/5 (927 download)

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Book Synopsis Computational Methods for Studying Gene Regulation and Genome Organization Using High-throughput DNA Sequencing by : Giancarlo A. Bonora

Download or read book Computational Methods for Studying Gene Regulation and Genome Organization Using High-throughput DNA Sequencing written by Giancarlo A. Bonora and published by . This book was released on 2015 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: The full sequencing of the human genome ushered in the genomics era and laid the foundation for a more comprehensive understanding of gene regulation and development. But, since the DNA sequence represents only one aspect of the genomic information housed within the nucleus, the question of exactly how it is utilized to direct developmental programs and tissue-specific gene expression is still an open one. However, rapid advances in high-throughput DNA sequencing (HTS) technologies over the past decade have allowed biologists to begin to tackle the question on a genomic scale. HTS has been coupled to bisulfite conversion of DNA for assessing cytosine methylation (bisulfite sequencing), to chromatin immunoprecipitation for ascertaining genomic locations bound by specific factors or found in a particular chromatin state (ChIP-seq), to the isolation of transcripts for the measurement of gene expression (RNA-seq), and to methods of chromosome conformation capture for the identification of genome-wide DNA-DNA interactions (4C-seq and Hi-C). The focus of my doctoral research has been the development of novel bioinformatics approaches to analyze the data produced by these technologies in order to shed light on how distinct cell identities are established and maintained. Here, I present highlights of this work in six chapters. Chapter 1 presents a study investigating DNA methylation changes going from the differentiated to pluripotent state, which shows that changes predominantly occur late in the process and are strongly associated with changes to chromatin state. Chapter 2 introduces methylation-sensitive restriction enzyme bisulfite sequencing (MREBS) as a method for assessing precise differential DNA methylation at cost comparable to RRBS, while providing additional information over a coverage area more comparable to WGBS. Chapter 3 presents a study showing that inhibition of ribonucleotide reductase decreased DNA methylation genome-wide by enhancing the incorporation of a cytidine analog into DNA. Chapter 4 describes a study showing that, for genes important to leaf senescence, temporal changes in expression closely matched changes to two histone modifications. Chapter 5 reviews cutting-edge research exploring the link between regulatory networks and genome organization. Chapter 6 describes a study showing that regulators responsible for cell identity contribute to cell type-specific genome organization.

Systems Biology

Author : Nikolaus Rajewsky
Publisher : Springer
ISBN 13 : 3319929674
Total Pages : 404 pages
Book Rating : 4.3/5 (199 download)

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Book Synopsis Systems Biology by : Nikolaus Rajewsky

Download or read book Systems Biology written by Nikolaus Rajewsky and published by Springer. This book was released on 2018-08-29 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many breakthroughs in experimental devices, advanced software, as well as analytical methods for systems biology development have helped shape the way we study DNA, RNA and proteins, on the genomic, transcriptional, translational and posttranslational level. This book highlights the comprehensive topics that encompass systems biology with enormous progress in the development of genome sequencing, proteomic and metabolomic methods in designing and understanding biological systems. Topics covered in this book include fundamentals of modelling networks, circuits and pathways, spatial and multi cellular systems, image-driven systems biology, evolution, noise and decision-making in single cells, systems biology of disease and immunology, and personalized medicine. Special attention is paid to epigenomics, in particular environmental conditions that impact genetic background. The breadth of exciting new data towards discovering fundamental principles and direct application of epigenetics in agriculture is also described. The chapter “Deciphering the Universe of RNA Structures and Trans RNA-RNA Interactions of Transcriptomes in vivo - from Experimental Protocols to Computational Analyses” is available open access under a CC BY 4.0 license via link.springer.com.

Cancer Genomics

Author : Ryan D. Morin
Publisher : Elsevier Inc. Chapters
ISBN 13 : 0128061006
Total Pages : 47 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Cancer Genomics by : Ryan D. Morin

Download or read book Cancer Genomics written by Ryan D. Morin and published by Elsevier Inc. Chapters. This book was released on 2013-11-21 with total page 47 pages. Available in PDF, EPUB and Kindle. Book excerpt: Identifying gene expression changes in cancer provides opportunities to identify biomarkers that can be informative in regard to risk and in the choice of treatment options. In recent years, advances in sequencing have provided not only a quantitative measure of gene expression, but the resolution of diverse species of RNA, alternative transcripts and allele-specific expression. As well, such data have revealed novel sequences such as those from pathogens, mutations resulting in amino acid differences and fusion transcripts resulting from translocations and other structural alterations, each of which can inform the development of novel treatment strategies or potential preventive measures. In this chapter, we will discuss how transcriptome sequencing is conducted and analyzed and provide examples that illustrate its utility in studying cancer samples.

Epigenomics

Author : Krishnarao Appasani
Publisher : Cambridge University Press
ISBN 13 : 1139536281
Total Pages : 549 pages
Book Rating : 4.1/5 (395 download)

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Book Synopsis Epigenomics by : Krishnarao Appasani

Download or read book Epigenomics written by Krishnarao Appasani and published by Cambridge University Press. This book was released on 2012-08-02 with total page 549 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding mechanisms of gene regulation that are independent of the DNA sequence itself - epigenetics - has the potential to overthrow long-held views on central topics in biology, such as the biology of disease or the evolution of species. High throughput technologies reveal epigenetic mechanisms at a genome-wide level, giving rise to epigenomics as a new discipline with a distinct set of research questions and methods. Leading experts from academia, the biotechnology and pharmaceutical industries explain the role of epigenomics in a wide range of contexts, covering basic chromatin biology, imprinting at a genome-wide level, and epigenomics in disease biology and epidemiology. Details on assays and sequencing technology serve as an up-to-date overview of the available technological tool kit. A reliable guide for newcomers to the field as well as experienced scientists, this is a unique resource for anyone interested in applying the power of twenty-first-century genomics to epigenetic studies.

The Analysis of Regulatory DNA: Current Developments, Knowledge and Applications Uncovering Gene Regulation

Author : Kenneth Berendzen
Publisher : Bentham Science Publishers
ISBN 13 : 1608054926
Total Pages : 225 pages
Book Rating : 4.6/5 (8 download)

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Book Synopsis The Analysis of Regulatory DNA: Current Developments, Knowledge and Applications Uncovering Gene Regulation by : Kenneth Berendzen

Download or read book The Analysis of Regulatory DNA: Current Developments, Knowledge and Applications Uncovering Gene Regulation written by Kenneth Berendzen and published by Bentham Science Publishers. This book was released on 2013-10-29 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: A major goal of integrative research is understanding regulatory networks to such an extent as to allow researchers to model developmental and stress responses. Regulatory networks of living systems include complex and vast interactions between proteins, metabolites, RNA, various signaling molecules and DNA. One aspect of systems biology is understanding the dynamics of protein-DNA interactions affecting gene expression that are caused by transcription factors (TFs) and chromatin remodeling factors. This e-book provides a resource for summarizing current knowledge eukaryotic transcription and explores cis-elements and methods for their analysis, prediction and discovery. The book also presents an overview of exploring gene regulatory networks, chromatin, and miRNAs. Information about state-of-the-art techniques for the determination of TF - cis-element interactions in vivo and in silico give cutting edge insights on how genomic-scale research is being approached. The Analysis of Regulatory DNA provides readers with both the necessary background knowledge and provocative, up-to-date insights aimed at sparking new and vibrant experimental designs for understanding and predicting cis-elements in the eukaryotic genome.