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Author : Michael P. Weiner
Publisher : Biotechniques Press
ISBN 13 :
Total Pages : 652 pages
Book Rating : 4.3/5 (97 download)
Download or read book Gene Cloning and Expression Technologies written by Michael P. Weiner and published by Biotechniques Press. This book was released on 2002 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sue Carson
Publisher : Elsevier
ISBN 13 : 0080456545
Total Pages : 172 pages
Book Rating : 4.0/5 (84 download)
Download or read book Manipulation and Expression of Recombinant DNA written by Sue Carson and published by Elsevier. This book was released on 2005-12-15 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : Karl Drlica
Publisher : John Wiley & Sons
ISBN 13 : 9780471622253
Total Pages : 276 pages
Book Rating : 4.6/5 (222 download)
Download or read book Understanding DNA and Gene Cloning written by Karl Drlica and published by John Wiley & Sons. This book was released on 1992 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses, or as a main text in short life sciences courses for non-science majors.
Author : Dr. RAVISHANKAR B.V.
Publisher : Lulu.com
ISBN 13 : 138715592X
Total Pages : 214 pages
Book Rating : 4.3/5 (871 download)
Download or read book GENE CLONING AND GENOMICS (Principles and Applications) written by Dr. RAVISHANKAR B.V. and published by Lulu.com. This book was released on 2017-09-04 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Deoxyribonucleic acid (DNA) is the genetic material of cells. It carries information in a coded form from cell to cell and from parent to offspring. A gene is a linear array of nucleotides located in a particular position on a particular chromosome that encodes a specific functional product (a protein or RNA molecule). When a gene is active, its information is copied first into another nucleic acid, ribonucleic acid (RNA), which in turn directs the synthesis of the gene products, the specific proteins. This lecture introduces some basic concepts of DNA, proteins, genes and genomes.
Author : Kakoli Bose
Publisher : Springer Nature
ISBN 13 : 9811649871
Total Pages : 315 pages
Book Rating : 4.8/5 (116 download)
Download or read book Textbook on Cloning, Expression and Purification of Recombinant Proteins written by Kakoli Bose and published by Springer Nature. This book was released on 2022-01-25 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
Author : T. A. Brown
Publisher : John Wiley & Sons
ISBN 13 : 1118685288
Total Pages : 337 pages
Book Rating : 4.1/5 (186 download)
Download or read book Gene Cloning and DNA Analysis written by T. A. Brown and published by John Wiley & Sons. This book was released on 2013-04-25 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "... the book content is elegantly illustrated and well organized in clear-cut chapters and subsections... there is a Further Reading section after each chapter that contains several key references... What is extremely useful, almost every reference is furnished with the short but distinct author's remark." –Journal of Heredity, 2007 (on the previous edition)
Author : François Baneyx
Publisher : Garland Science
ISBN 13 : 9780954523251
Total Pages : 546 pages
Book Rating : 4.5/5 (232 download)
Download or read book Protein Expression Technologies written by François Baneyx and published by Garland Science. This book was released on 2004 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in protein expression technologies have mushroomed in recent years. In this book current and emerging expression technologies are reviewed. Reviews of the molecular genetics of expression systems in various organisms are presented. Topics covered include: Expression of extremophilic proteins; expression in E. coli, Bacillus spp., Saccharomyces cerevisiae, and methylotrophic yeasts; insect cell expression and the baculovirus system; and Chinese Hamster Ovary (CHO) cell lines for large-scale protein production. Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus.
Author : Siddra Ijaz
Publisher : Cambridge Scholars Publishing
ISBN 13 : 1527538443
Total Pages : 156 pages
Book Rating : 4.5/5 (275 download)
Download or read book Recombinant DNA Technology written by Siddra Ijaz and published by Cambridge Scholars Publishing. This book was released on 2019-08-13 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.
Author : B.C. Schaefer
Publisher : Taylor & Francis
ISBN 13 : 1000943445
Total Pages : 213 pages
Book Rating : 4.0/5 (9 download)
Download or read book Gene Cloning and Analysis written by B.C. Schaefer and published by Taylor & Francis. This book was released on 2023-04-28 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on newly emerging technologies that facilitate the isolation and characterization of genes. The detailed protocols will be useful to the seasoned professional and easily understood by the novice. The vast majority of methods are applic
Author : P.C. Huang
Publisher : Elsevier
ISBN 13 : 0323153577
Total Pages : 375 pages
Book Rating : 4.3/5 (231 download)
Download or read book Genetic Engineering Techniques: Recent Developments written by P.C. Huang and published by Elsevier. This book was released on 2012-12-02 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic Engineering Techniques: Recent Developments covers the proceedings of the 1982 Genetic Engineering Techniques symposium held in Taipei. The book is organized into 21 chapters that discuss the application of recombination DNA methods in the study of DNA structure and DNA-protein interactions; the use of chemically synthesized genes in cloning; and gene expression. After briefly presenting the major strategies underlying genetic engineering technology and rapid method for sequencing DNA, the book examines the reaction mechanism of a multifunctional Type I enzyme and the organization and expression of the human adenovirus. The second section describes several approaches in analyzing transcriptional processes in prokaryotic and eukaryotic systems. This section also deals with cloning vectors and procedures of cDNA. The subsequent section describes a molecular approach to functional analysis of the influenza virus surface hemagglutinin; the transposition specificity for the transposons 3 and 4 elements; and the biological properties of human T-cell growth factor gene. The fourth section discusses the principles of hybridoma technology and its numerous applications to biological research. The remaining chapters of the book present laboratory courses designed to familiarize researchers with the principles and basic procedures in biological experiments. Genetic engineering researchers, agriculturists, and geneticists will find this book invaluable.
Author : Jeremy W. Dale
Publisher : John Wiley & Sons
ISBN 13 : 0470683864
Total Pages : 401 pages
Book Rating : 4.4/5 (76 download)
Download or read book From Genes to Genomes written by Jeremy W. Dale and published by John Wiley & Sons. This book was released on 2011-12-12 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of this highly successful textbook introduces the key techniques and concepts involved in cloning genes and in studying their expression and variation. The new edition features: Increased coverage of whole-genome sequencing technologies and enhanced treatment of bioinformatics. Clear, two-colour diagrams throughout. A dedicated website including all figures. Noted for its outstanding balance between clarity of coverage and level of detail, this book provides an excellent introduction to the fast moving world of molecular genetics.
Author : Stephen D. Fairbanks
Publisher : Nova Publishers
ISBN 13 : 9781590338797
Total Pages : 252 pages
Book Rating : 4.3/5 (387 download)
Download or read book Cloning written by Stephen D. Fairbanks and published by Nova Publishers. This book was released on 2004 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: The terms 'recombinant DNA technology', 'DNA cloning', 'molecular cloning' or 'gene cloning' all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal. Dolly was created by reproductive cloning technology. In a process called 'somatic cell nuclear transfer' (SCNT), scientists transfer genetic material from the nucleus of a donor adult cell to an egg whose nucleus, and thus its genetic material, has been removed. The reconstructed egg containing the DNA from a donor cell must be treated with chemicals or electric current in order to stimulate cell division. Once the cloned embryo reaches a suitable stage, it is transferred to the uterus of a female host where it continues to develop until birth. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in research. The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease. Stem cells are important to biomedical researchers because they can be used to generate virtually any type of specialised cell in the human body. This new book presents an up-to-date Chronology of Cloning along with current and selected abstracts dealing with cloning as well as a guide to books on the topic. Access to the abstract and books sections is provided by title, subject and author indexes.
Author : Lesley Tillman
Publisher : Scientific e-Resources
ISBN 13 : 1839471611
Total Pages : 344 pages
Book Rating : 4.8/5 (394 download)
Download or read book Gene Cloning written by Lesley Tillman and published by Scientific e-Resources. This book was released on with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene cloning is the act of making copies, or clones, of a single gene. Once a gene is identified, clones can be used in many areas of biomedical and industrial research. Genetic engineering is the process of cloning genes into new organisms for altering the DNA sequence to change the protein product. Genetic engineering depends on our ability to perform the following essential procedures. Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. The available information on gene cloning and transgenic development in horticulture crops has been compiled and it is hoped that this would be very useful to students and researchers in the field of biotechnology of horticulture crops. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained, then the foreign DNA will be replicated along with the host cell's DNA in the transgenic organism. The book has been designed for students, research scholars and teachers involved in the field
Author : Julia Lodge
Publisher : Garland Science
ISBN 13 : 1135728925
Total Pages : 612 pages
Book Rating : 4.1/5 (357 download)
Download or read book Gene Cloning written by Julia Lodge and published by Garland Science. This book was released on 2007-01-24 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.
Author : Terence A. Brown
Publisher :
ISBN 13 :
Total Pages : 374 pages
Book Rating : 4.3/5 (91 download)
Download or read book Gene Cloning written by Terence A. Brown and published by . This book was released on 1995 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Yechiel Becker
Publisher : Springer Science & Business Media
ISBN 13 : 146132565X
Total Pages : 372 pages
Book Rating : 4.4/5 (613 download)
Download or read book Recombinant DNA Research and Viruses written by Yechiel Becker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of recombinant DNA technology has made a marked impact on molecular virology. The cleavage of viral DNA genomes with restriction enzymes and the cloning of such DNA fragments in bacterial p1asmids has led to the amplification of selected viral DNA fragments for sequencing and gene expression. RNA virus genomes which can be transcribed to their cDNA form were also cloned in bacterial p1asmids, facilitating the study of RNA virus genes. With the elucidation in recent years of the promoter sequence of various viral genes and the expression of these genes in bacteria or yeast, the understanding of many viral gene functions has made great progress. Cloning and expression of viral genes in mammalian cells was made possible by the construction of shuttle plasmid vectors which carry the origins of DNA replication from bacteria and/or mammalian viruses. The expression of viral genes in bacteria, yeast and eukaryotic cells gives reason to hope that it will be possible to produce viral antigens in large quantities for use as human or animal vaccines. The present volume attempts to capture for the reader some of the high lights of recombinant DNA research in the field of animal and plant viruses.
Author : Sandy B. Primrose
Publisher : John Wiley & Sons
ISBN 13 : 1405135441
Total Pages : 675 pages
Book Rating : 4.4/5 (51 download)
Download or read book Principles of Gene Manipulation and Genomics written by Sandy B. Primrose and published by John Wiley & Sons. This book was released on 2006-02-10 with total page 675 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increasing integration between gene manipulation and genomics is embraced in this new book, Principles of Gene Manipulation and Genomics, which brings together for the first time the subjects covered by the best-selling books Principles of Gene Manipulation and Principles of Genome Analysis & Genomics. Comprehensively revised, updated and rewritten to encompass within one volume, basic and advanced gene manipulation techniques, genome analysis, genomics, transcriptomics, proteomics and metabolomics Includes two new chapters on the applications of genomics An accompanying website - www.blackwellpublishing.com/primrose - provides instructional materials for both student and lecturer use, including multiple choice questions, related websites, and all the artwork in a downloadable format. An essential reference for upper level undergraduate and graduate students of genetics, genomics, molecular biology and recombinant DNA technology.
