Functional Analysis of Nuclear-Encoded Mitochondrial Gene Duplicates with Testis-Biases Expression in Drosophila Melanogaster

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ISBN 13 :
Total Pages : 156 pages
Book Rating : 4.:/5 (132 download)

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Book Synopsis Functional Analysis of Nuclear-Encoded Mitochondrial Gene Duplicates with Testis-Biases Expression in Drosophila Melanogaster by : Mohammadmehdi M Eslamieh

Download or read book Functional Analysis of Nuclear-Encoded Mitochondrial Gene Duplicates with Testis-Biases Expression in Drosophila Melanogaster written by Mohammadmehdi M Eslamieh and published by . This book was released on 2022 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most of the genes encoding proteins that function in the mitochondria are located in the nucleus and are called nuclear-encoded mitochondrial genes (N-mt genes). In Drosophila melanogaster, most of the tissue-specific duplicated N-mt genes have acquired testis-biased expression. These genes appear to be older and relocated more often than other duplicated genes and have energy-related functions. These patterns reveal strong selection for the retention of new genes for male germline mitochondrial functions. Maternal inheritance of mitochondria and male-male competition for fertilization are two common forces that might drive changes in mitochondrial functions. We analyzed N-mt genes in the human genome to test the presence of this pattern in other species. We find that about 18% of human N-mt genes fall into gene families, but unlike in Drosophila, only 28% of the N-mt duplicates have tissue-biased expression and only 36% of these have testis-biased expression. To study the function of the Drosophila testis-biased duplicates, we knocked down 39 N-mt duplicated genes with testis-biased expression in D. melanogaster. Multiple RNAi lines and a few TRiP-CRISPR knockout lines were used to study the fertility effects of these genes. Forty-nine percent of the RNAi knockdowns in germline showed male sterility and seventy-two percent of TRiP-CRISPR knockout lines and all few previously published knockout studies confirmed these results. We have failed to find any evidence of the parental gene being able to compensate for the N-mt duplicated gene in the fertility effects. These effects along with the different pattern of expression that leads to the presence of these genes in the sperm proteome/mitochondria, their protein divergence, and positive selection inferred for some of these genes suggest that the new duplicate genes have a different function than their parental genes during spermatogenesis and/or in sperm. We also produced a knockout mutant for Cytochrome c oxidase 4-like (COX4L) in D. melanogaster to understand the function of this new gene in detail. The knockout strain for this gene was generated using CRISPR-Cas9 technology. The knockout of COX4L produce completely sterile males unlike the knockdown that produced partial male sterility and reveals that knockdown results might have milder phenotypes than knockouts as expected. A lack of sperm individualization is observed in those males. The male infertility is rescued by driving COX4L-HA in the germline. In addition, ectopic expression of COX4L in soma caused embryonic lethality as expected, if COX4L has a specialized male germline function. Overexpression of COX4L in male germline leads to a reduction in male fertility revealing that fine tuning of the expression of this gene is needed. Several stainings revealed that the mitochondria of COX4L-KO are not functional and the ROS production in the testes was higher than in controls which might explain the male sterility of these flies. This prominent phenotype along with having energy-related functions, testis-biased expression, and also being present in the Drosophila sperm proteome database supports the idea that males might use different mitochondria in their germline and/or sperm.

Genetics Abstracts

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ISBN 13 :
Total Pages : 674 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Genetics Abstracts by :

Download or read book Genetics Abstracts written by and published by . This book was released on 1996 with total page 674 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Genes in Conflict

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Publisher : Harvard University Press
ISBN 13 : 0674029119
Total Pages : 613 pages
Book Rating : 4.6/5 (74 download)

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Book Synopsis Genes in Conflict by : Austin BURT

Download or read book Genes in Conflict written by Austin BURT and published by Harvard University Press. This book was released on 2009-06-30 with total page 613 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering all species from yeast to humans, this is the first book to tell the story of selfish genetic elements that act narrowly to advance their own replication at the expense of the larger organism.

Sex Chromosomes and Sex-Linked Genes

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Publisher : Springer Science & Business Media
ISBN 13 : 3642881785
Total Pages : 201 pages
Book Rating : 4.6/5 (428 download)

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Book Synopsis Sex Chromosomes and Sex-Linked Genes by : Susumu Ohno

Download or read book Sex Chromosomes and Sex-Linked Genes written by Susumu Ohno and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Natural selection operates among individual organisms which differ in their genetic constitution. The degree of hereditary variability within a species is greatly enhanced by cross-fertilization. Indeed, the mechanism of sexual reproduction occurred very early in evolution, for it is seen today even in bacteria. In Escherichia coli, fertilization occurs by passage of the single chromosome from the male into the female bacterium (LEDERBERG, 1959). In multicellular organisms, the separation of germ from soma, and the production of haploid gametes became mandatory. The gametes were of two types. One, extremely mobile, was designed to seek out and penetrate the other, which loaded with nutrients, received the mobile gamete and intiated the development of a new individual. The foundation for true bisexuality was thus laid. In the primitive state of bisexuality, whether an individual is to be a sperm-producing male or an egg-producing female appears to be decided rather haphazardly. In the worm, Banelia viridis, the minute males are parasites in the female. Larvae that become attached to the proboscis of an adult female become males, while unattached larvae sink to the bottom and become females (BALTZER, 1935). The more sophisticated state of bisexuality was initiated by setting aside a particular pair of chromosomes for specialization and making either the male or the female a heterogametic sex. Sex chromosomes as we know them were thus born.

Molecular Biology and Biotechnology of Plant Organelles

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Publisher : Springer Science & Business Media
ISBN 13 : 1402031661
Total Pages : 671 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Molecular Biology and Biotechnology of Plant Organelles by : Henry Daniell, Ph.D.

Download or read book Molecular Biology and Biotechnology of Plant Organelles written by Henry Daniell, Ph.D. and published by Springer Science & Business Media. This book was released on 2007-11-04 with total page 671 pages. Available in PDF, EPUB and Kindle. Book excerpt: We have taught plant molecular biology and biotechnology at the undergraduate and graduate level for over 20 years. In the past few decades, the field of plant organelle molecular biology and biotechnology has made immense strides. From the green revolution to golden rice, plant organelles have revolutionized agriculture. Given the exponential growth in research, the problem of finding appropriate textbooks for courses in plant biotechnology and molecular biology has become a major challenge. After years of handing out photocopies of various journal articles and reviews scattered through out the print and electronic media, a serendipitous meeting occurred at the 2002 IATPC World Congress held in Orlando, Florida. After my talk and evaluating several posters presented by investigators from my laboratory, Dr. Jacco Flipsen, Publishing Manager of Kluwer Publishers asked me whether I would consider editing a book on Plant Organelles. I accepted this challenge, after months of deliberations, primarily because I was unsuccessful in finding a text book in this area for many years. I signed the contract with Kluwer in March 2003 with a promise to deliver a camera-ready textbook on July 1, 2004. Given the short deadline and the complexity of the task, I quickly realized this task would need a co-editor. Dr. Christine Chase was the first scientist who came to my mind because of her expertise in plant mitochondria, and she readily agreed to work with me on this book.

The Evolution of Sex Determination

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Publisher : OUP Oxford
ISBN 13 : 019163140X
Total Pages : 455 pages
Book Rating : 4.1/5 (916 download)

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Book Synopsis The Evolution of Sex Determination by : Leo Beukeboom

Download or read book The Evolution of Sex Determination written by Leo Beukeboom and published by OUP Oxford. This book was released on 2014-06-12 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sexual reproduction is a fundamental aspect of life. It is defined by the occurrence of meiosis and the fusion of two gametes of different sexes or mating types. Sex-determination mechanisms are responsible for the sexual fate and development of sexual characteristics in an organism, be it a unicellular alga, a plant, or an animal. In many cases, sex determination is genetic: males and females have different alleles or different genes that specify their sexual morphology. In animals, this is often accompanied by chromosomal differences. In other cases, sex may be determined by environmental (e.g. temperature) or social variables (e.g. the size of an organism relative to other members of its population). Surprisingly, sex-determination mechanisms are not evolutionarily conserved but are bewilderingly diverse and appear to have had rapid turnover rates during evolution. Evolutionary biologists continue to seek a solution to this conundrum. What drives the surprising dynamics of such a fundamental process that always leads to the same outcome: two sex types, male and female? The answer is complex but the ongoing genomic revolution has already greatly increased our knowledge of sex-determination systems and sex chromosomes in recent years. This novel book presents and synthesizes our current understanding, and clearly shows that sex-determination evolution will remain a dynamic field of future research. The Evolution of Sex Determination is an advanced, research level text suitable for graduate students and researchers in genetics, developmental biology, and evolution.

Ribonucleases H

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ISBN 13 : 9782855985978
Total Pages : 265 pages
Book Rating : 4.9/5 (859 download)

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Book Synopsis Ribonucleases H by : Robert J.. Crouch

Download or read book Ribonucleases H written by Robert J.. Crouch and published by . This book was released on 1998-01-01 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Introduction to Epigenetics

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Publisher : Springer Nature
ISBN 13 : 3030686701
Total Pages : 215 pages
Book Rating : 4.0/5 (36 download)

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Book Synopsis Introduction to Epigenetics by : Renato Paro

Download or read book Introduction to Epigenetics written by Renato Paro and published by Springer Nature. This book was released on 2021-03-23 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access textbook leads the reader from basic concepts of chromatin structure and function and RNA mechanisms to the understanding of epigenetics, imprinting, regeneration and reprogramming. The textbook treats epigenetic phenomena in animals, as well as plants. Written by four internationally known experts and senior lecturers in this field, it provides a valuable tool for Master- and PhD- students who need to comprehend the principles of epigenetics, or wish to gain a deeper knowledge in this field. After reading this book, the student will: Have an understanding of the basic toolbox of epigenetic regulation Know how genetic and epigenetic information layers are interconnected Be able to explain complex epigenetic phenomena by understanding the structures and principles of the underlying molecular mechanisms Understand how misregulated epigenetic mechanisms can lead to disease

Molecular Mechanisms of Notch Signaling

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Publisher : Springer
ISBN 13 : 3319895125
Total Pages : 417 pages
Book Rating : 4.3/5 (198 download)

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Book Synopsis Molecular Mechanisms of Notch Signaling by : Tilman Borggrefe

Download or read book Molecular Mechanisms of Notch Signaling written by Tilman Borggrefe and published by Springer. This book was released on 2018-07-20 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the Notch signaling pathway with a focus on molecular mechanisms. The Notch signaling pathway is a seemingly simple pathway that does not involve any second messenger. Upon ligand binding two consecutive proteolytic cleavages of the NOTCH receptor release the Notch intracellular domain from the membrane. The Notch intracellular domain migrates into the nucleus and activates gene expression. Recently, new technologies allowed us to better understand this pivotal signaling cascade and revealed new regulatory mechanisms. The different chapters cover many aspects of the Notch signaling focusing on the mechanisms governing the receptor/ligand interaction as well as on the downstream intracellular signaling events. Aspects of both canonical and non-canonical signaling are discussed and the function of Notch signaling in physiological and pathological contexts are elucidated. This book is not only intended for experts but it should also be a useful resource for young, sprouting scientists or interested scientists from other research areas, who may use this book as a stimulating starting point for further discoveries and developments.

Atlas of Drosophila Development

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ISBN 13 :
Total Pages : 72 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Atlas of Drosophila Development by : Volker Hartenstein

Download or read book Atlas of Drosophila Development written by Volker Hartenstein and published by . This book was released on 1993 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt: This full-color atlas graphically documents the main events of embryonic and post-embryonic development in Drosophila. Schematic surface views and transverse sections from several developmental stages are shown for the individual organs such as gut, nervous system, epidermis and musculature. By combining camera lucida tracing with digital technology, Volker Hartenstein has created a unique, beautiful and convenient reference book that will interest all developmental biologists and is a must for the personal library of anyone working on fly biology.

Bioinformatics of Genome Regulation and Structure

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Publisher : Springer Science & Business Media
ISBN 13 : 1441971521
Total Pages : 373 pages
Book Rating : 4.4/5 (419 download)

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Book Synopsis Bioinformatics of Genome Regulation and Structure by : Nikolay Kolchanov

Download or read book Bioinformatics of Genome Regulation and Structure written by Nikolay Kolchanov and published by Springer Science & Business Media. This book was released on 2013-06-05 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: - The data gathered can be used to solve a wide range of problems - for basic science and applied science

Genetics of Human Infertility

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Publisher : Karger Medical and Scientific Publishers
ISBN 13 : 3318060984
Total Pages : 190 pages
Book Rating : 4.3/5 (18 download)

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Book Synopsis Genetics of Human Infertility by : P.H. Vogt

Download or read book Genetics of Human Infertility written by P.H. Vogt and published by Karger Medical and Scientific Publishers. This book was released on 2017-09-12 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Infertility affects more than one in ten couples worldwide and is related to highly heterogeneous pathologies sometimes only discernible in the germ line. Its complex etiology often, but not always, includes genetic factors besides anatomical defects, immunological interference, and environmental aspects. Nearly 30% of infertility cases are probably caused only by genetic defects. Thereby experimental animal knockout models convincingly show that infertility can be caused by single or multiple gene defects. Translating those basic research findings into clinical studies is challenging, leaving genetic causes for the vast majority of infertility patients unexplained. Nevertheless, a large number of candidate genes have been revealed by sophisticated molecular methods. This book provides a comprehensive overview on the subject of infertility written by the leading authorities in this field. It covers topics including basic biological, cytological, and molecular studies, as well as common and uncommon syndromes. It is a must-read for human geneticists, endocrinologists, epidemiologists, zoologists, and counsellors in human genetics, infertility, and assisted reproduction.

Introduction to Computational Genomics

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Publisher : Cambridge University Press
ISBN 13 : 9780521856034
Total Pages : 200 pages
Book Rating : 4.8/5 (56 download)

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Book Synopsis Introduction to Computational Genomics by : Nello Cristianini

Download or read book Introduction to Computational Genomics written by Nello Cristianini and published by Cambridge University Press. This book was released on 2006-12-14 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: Where did SARS come from? Have we inherited genes from Neanderthals? How do plants use their internal clock? The genomic revolution in biology enables us to answer such questions. But the revolution would have been impossible without the support of powerful computational and statistical methods that enable us to exploit genomic data. Many universities are introducing courses to train the next generation of bioinformaticians: biologists fluent in mathematics and computer science, and data analysts familiar with biology. This readable and entertaining book, based on successful taught courses, provides a roadmap to navigate entry to this field. It guides the reader through key achievements of bioinformatics, using a hands-on approach. Statistical sequence analysis, sequence alignment, hidden Markov models, gene and motif finding and more, are introduced in a rigorous yet accessible way. A companion website provides the reader with Matlab-related software tools for reproducing the steps demonstrated in the book.

An Introduction to Ecological Genomics

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Publisher : Oxford University Press
ISBN 13 : 0199594686
Total Pages : 374 pages
Book Rating : 4.1/5 (995 download)

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Book Synopsis An Introduction to Ecological Genomics by : Nico M. van Straalen

Download or read book An Introduction to Ecological Genomics written by Nico M. van Straalen and published by Oxford University Press. This book was released on 2012 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors also provide a comparative survey of the properties of genomes (genome size, gene families, synteny, and polymorphism) for prokaryotes as well as the main eukaryotic models.

Bioinformatics for Beginners

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Publisher : Elsevier
ISBN 13 : 0124105106
Total Pages : 238 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Bioinformatics for Beginners by : Supratim Choudhuri

Download or read book Bioinformatics for Beginners written by Supratim Choudhuri and published by Elsevier. This book was released on 2014-05-09 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioinformatics for Beginners: Genes, Genomes, Molecular Evolution, Databases and Analytical Tools provides a coherent and friendly treatment of bioinformatics for any student or scientist within biology who has not routinely performed bioinformatic analysis. The book discusses the relevant principles needed to understand the theoretical underpinnings of bioinformatic analysis and demonstrates, with examples, targeted analysis using freely available web-based software and publicly available databases. Eschewing non-essential information, the work focuses on principles and hands-on analysis, also pointing to further study options. Avoids non-essential coverage, yet fully describes the field for beginners Explains the molecular basis of evolution to place bioinformatic analysis in biological context Provides useful links to the vast resource of publicly available bioinformatic databases and analysis tools Contains over 100 figures that aid in concept discovery and illustration

The Pangenome

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Publisher : Springer Nature
ISBN 13 : 3030382818
Total Pages : 311 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis The Pangenome by : Hervé Tettelin

Download or read book The Pangenome written by Hervé Tettelin and published by Springer Nature. This book was released on 2020-04-30 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book offers the first comprehensive account of the pan-genome concept and its manifold implications. The realization that the genetic repertoire of a biological species always encompasses more than the genome of each individual is one of the earliest examples of big data in biology that opened biology to the unbounded. The study of genetic variation observed within a species challenges existing views and has profound consequences for our understanding of the fundamental mechanisms underpinning bacterial biology and evolution. The underlying rationale extends well beyond the initial prokaryotic focus to all kingdoms of life and evolves into similar concepts for metagenomes, phenomes and epigenomes. The book’s respective chapters address a range of topics, from the serendipitous emergence of the pan-genome concept and its impacts on the fields of microbiology, vaccinology and antimicrobial resistance, to the study of microbial communities, bioinformatic applications and mathematical models that tie in with complex systems and economic theory. Given its scope, the book will appeal to a broad readership interested in population dynamics, evolutionary biology and genomics.

The Genetic Gods

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Publisher : Harvard University Press
ISBN 13 : 0674020359
Total Pages : 289 pages
Book Rating : 4.6/5 (74 download)

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Book Synopsis The Genetic Gods by : John C. Avise

Download or read book The Genetic Gods written by John C. Avise and published by Harvard University Press. This book was released on 2009-06-30 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: They mastermind our lives, shaping our features, our health, and our behavior, even in the sacrosanct realms of love and sex, religion, aging, and death. Yet we are the ones who house, perpetuate, and give the promise of immortality to these biological agents, our genetic gods. The link between genes and gods is hardly arbitrary, as the distinguished evolutionary geneticist John Avise reveals in this compelling book. In clear, straightforward terms, Avise reviews recent discoveries in molecular biology, evolutionary genetics, and human genetic engineering, and discusses the relevance of these findings to issues of ultimate concern traditionally reserved for mythology, theology, and religious faith. The book explains how the genetic gods figure in our development--not just our metabolism and physiology, but even our emotional disposition, personality, ethical leanings, and, indeed, religiosity. Yet genes are physical rather than metaphysical entities. Having arisen via an amoral evolutionary process--natural selection--genes have no consciousness, no sentient code of conduct, no reflective concern about the consequences of their actions. It is Avise's contention that current genetic knowledge can inform our attempts to answer typically religious questions--about origins, fate, and meaning. The Genetic Gods challenges us to make the necessary connection between what we know, what we believe, and what we embody. Table of Contents: Preface Prologue 1. The Doctrines of Biological Science 2. Geneses 3. Genetic Maladies 4. Genetic Beneficence 5. Strategies of the Genes 6. Genetic Sovereignty 7. New Lords of Our Genes? 8. Meaning Epilogue Notes Glossary Index Reviews of this book: Our genes, [Avise] says, are responsible not only for how we got here and exist day to day, but also for the core of our being--our personalities and morals. It is our genetic make-up that allows for and formulates our religious belief systems, he argues. Avise does not eschew spirituality but seeks a more informed, less confrontational approach between science and the pulpit. --Science News Reviews of this book: For the general scientific reader, the book is an excellent distillation of a broad and increasingly important field, a course of causation that cannot be ignored. From advising expectant parents to getting innocent people off death row, genetics increasingly dominates our lives. The sections on genetics are expertly written, particularly for those readers without in-depth knowledge. The author explains slowly and carefully just how genetics operates, using multiple metaphors. His genetic discourse proceeds in a neighborly fashion, as one might tell stories while sitting in a rocking chair at a country store. He seems to be invigorated by genes and just can't wait to tell about them. --David W. Hodo, Journal of the American Medical Association Reviews of this book: As a whole, this book is quite informative and stimulating, and sections of it are beautifully written. Indeed, Professor Avise has a real gift for prose and scientific expositions, and I would suspect that he must be a formidable lecturer...At its core, [The Genetic Gods] is a survey, and a very nice one at that, of evolutionary genetics, the field of the author's major research interests. There is a strong sociobiological cast to the arguments, and the work and ideas of E. O. Wilson figure prominently. The presentation of evolutionary genetics is imbedded in a more general discussion of modern human and molecular genetics...However, this book is, most of all, a philosophical treatise that attempts, admittedly with the bias of a biologist, to examine the intersection of the fundamental premises of evolution and religion. Professor Avise has given us plenty to think about in this book [and]...it was a real pleasure to wrestle with the ideas he was presenting. I would suggest that other readers give it a try. --Charles J. Epstein, Trends in Genetics Reviews of this book: [Avise's] account of the role genes play in shaping the human condition is wholly involving, paying particular attention to issues of reproduction, aging and death. In addition to presenting ample biological information in a form accessible to the nonspecialist, Avise does a superb job of discussing many of the ethical implications that have arisen from our growing knowledge of human genetics. Just a few of the topics covered are genetic engineering, the patenting of life, genetic screening, abortion, human cloning, gene therapy and insurance-related controversies. --Publishers Weekly Reviews of this book: Avise explains thoroughly how evolution operates on a genetic level. His goal is to show that humans can look to this information as a way to answer fundamental questions of life instead of looking to traditional religious beliefs...Avise includes some very interesting discussions of ethical concerns related to genetic issues. --Eric D. Albright, Library Journal This is a splendid account of a subject that affects us all: the breathtaking increase in understanding of human genetics and the insight it provides into human evolution. John Avise speaks with authority of molecular evolutionary genetics and with affecting compassion of what it might mean. --Douglas J. Futuyma, State University of New York at Stony Brook The Genetic Gods is many things. It is a wonderful introduction to modern molecular biology, by a man who knows his subject backwards. It is a stimulating account of the ways in which genetics impinges on human nature--our thinking and our behavior. It is a remarkably level-headed and sympathetic account of the implications of our new findings for traditional and not-so-traditional issues in philosophy and religion. In an age of genetic counseling, cloning, construction of new life forms, the book is worth its weight in gold for this alone. But most of all, it is a huge amount of fun to read--you want to applaud or argue with the author on nigh every page. Highly recommended! --Michael Ruse, University of Guelph The Genetic Gods makes a valuable contribution to the on-going task of sorting out the implications of evolutionary biology and genetics for human self-understanding. Avise addresses, with authority and grace, the most consequential intellectual issues of our time. A challenging and insightful book. --Loyal Rue, Harvard University A wonderfully informative and engaging book. Avise offers a lucid, accessible primer on our genes, angelic and demonic, and examines religious and ethical issues, all too human, now confronted by genetic science. He makes a compelling case that anyone seeking to 'Know Thyself' should study the DNA molecular scriptures, our most ancient and universal legacy. --Dudley Herschbach, Harvard University, Nobel Laureate in Chemistry