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Freeze Fracture Studies Of Membranes
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Book Synopsis Freeze-Fracture Studies of Membranes by : Sek Wen Hui
Download or read book Freeze-Fracture Studies of Membranes written by Sek Wen Hui and published by CRC Press. This book was released on 2018-01-18 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.
Book Synopsis Freeze-fracture Studies of Protein-lipid Interactions in Membranes by : Wolfgang Kleemann
Download or read book Freeze-fracture Studies of Protein-lipid Interactions in Membranes written by Wolfgang Kleemann and published by . This book was released on 1975 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Freeze-fracture Studies on Yeast Plasma Membranes by : Wouterus Thomas Maria Gruijters
Download or read book Freeze-fracture Studies on Yeast Plasma Membranes written by Wouterus Thomas Maria Gruijters and published by . This book was released on 1985 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Freeze Fracture written by John E. Rash and published by . This book was released on 1979 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Studies of Membrane Structure by Freeze-etching. Progress Report, 1 July 1975--30 June 1976. [Erythrocyte Membranes]. by :
Download or read book Studies of Membrane Structure by Freeze-etching. Progress Report, 1 July 1975--30 June 1976. [Erythrocyte Membranes]. written by and published by . This book was released on 1976 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The structure and organization of biological membranes were studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts have been probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and electron microscope autoradiographic techniques has been developed and tested and a new method of rotary shadow freeze-etching has been evaluated. The role of lipid-lipid and protein-protein interactions in controlling the mobility of cell surface macromolecules has been studied using freeze-fracture, protein extraction and SDS-polyacrylamide gel techniques.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Studies of Membrane Structure by Freeze-etching. Progress Report by :
Download or read book Studies of Membrane Structure by Freeze-etching. Progress Report written by and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The researchers devised a rapid procedure for measuring covalent bond breakage in a simple model system to determine the extent to which glycophorin was cleaved when the frozen vesicles were fractured. Evidence showed that no covalent bonds are broken during the fracture procedure. Membrane splitting was shown to be quantified under special circumstances when erythrocyte membranes are appressed to a polylysine-coated surface. Results show that freeze fracture at temperatures below that of liquid nitrogen results in the appearance of considerable detail on E faces of purple membranes. Detail seems to improve as the temperature is lowered, suggesting that the smooth E faces in conventional freeze fractures may be a result of plastic deformation and flow of membrane components. Results show that ultra-low temperature freeze-fracturing can reduce plastic distortion and, particularly when used in conjunction with rotary replication, should improve our ability to discern small labels attached to transmembrane proteins. (PCS).
Book Synopsis Studies of Membrane Structure by Freeze-etching. Progress Report, October 1, 1977--October 31, 1978 by :
Download or read book Studies of Membrane Structure by Freeze-etching. Progress Report, October 1, 1977--October 31, 1978 written by and published by . This book was released on 1978 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The structure and organization of biological membranes is being studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts are being probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and biochemical techniques using fluorescent resonance energy transfer has been developed and tested and ultra low temperature freeze-etching has been evaluated as part of a program to develop covalent labelling techniques that will identify specific transmembrane proteins in freeze-fractured replicas.
Book Synopsis Studies of Membrane Structure by Freeze-etching. Progress Report, 1 July 1976--30 June 1977 by :
Download or read book Studies of Membrane Structure by Freeze-etching. Progress Report, 1 July 1976--30 June 1977 written by and published by . This book was released on 1977 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The structure and organization of biological membranes has been studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts have been probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and biochemical techniques using fluorescent resonance energy transfer has been developed and tested and ultra low temperature freeze-etching has been evaluated as part of a program to develop covalent labelling techniques that will identify specific transmembrane proteins in freeze-fractured replicas.
Book Synopsis Freeze-fracture studies on lipid polymorphism by : Rudolf van Venetië
Download or read book Freeze-fracture studies on lipid polymorphism written by Rudolf van Venetië and published by . This book was released on 1982 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis An Introduction to Biological Membranes by : William Stillwell
Download or read book An Introduction to Biological Membranes written by William Stillwell and published by Newnes. This book was released on 2013-04-20 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. Brings together different facets of membrane research in a universally understandable manner Emphasis on the historical development of the field Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport
Book Synopsis Biomembrane Structures by : Parvez I. Haris
Download or read book Biomembrane Structures written by Parvez I. Haris and published by IOS Press. This book was released on 1998 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes play a significant role in a range of biological processes such as ion-transport and signal transduction. Over the years much effort has been devoted towards developing an understanding of biomembrane structure. The study of this subject is now reaching an important stage. This is because at last the full three-dimensional structure of certain membrane proteins is beginning to be resolved. In the past three-dimensional structures of membrane proteins were difficult to obtain as only two dimensional crystals were available. In recent years satisfactory crystals have been obtained and X-ray diffraction techniques have been applied. This has led to the three dimensional structures of the photosynthetic reaction centres, porins and more recently the structure of cytochrome oxidase. Of course not all membrane proteins are readily crystallisable and some are not even available in sufficient quantities to obtain the necessary crystals or to carry out biophysical experiments. In some cases e.g. the voltage-gated potassium ion channel membrane proteins their structure has been proposed mainly on the basis of molecular biology methods. This has prompted the search for alternative approaches for characterising biomembrane structure. Molecular biological studies are providing a wealth of information on a number of different membrane proteins. Combining the information derived from such studies with molecular modelling is becoming extremely useful for relating structure to function. Development of other approaches include synthesis and structure- function analysis of peptides corresponding to functionally important domains of membrane proteins. This book presents a series of Chapters discussing how a combination of molecular biological, biophysical and theoretical (molecular modelling) techniques are helping us to obtain a much clearer picture of biomembrane structure. After an introductory Chapter on the Principles of membrane Protein Structure, the book is divided into two sections; one dealing with crystallographic approaches and the other non-crystallographic approaches such as NMR, AFM, SPR and FTIR spectroscopy. Chapters dealing with the recently solved crystal structure of cytochrome oxidase and bacteriorhodopsin are presented. The book contains contributions from leading membrane scientists describing their latest studies. It provides an up to date coverage of the developments in the field of biomembranes with particular emphasis on membrane proteins.
Book Synopsis Techniques in Lipid and Membrane Biochemistry by : M. J. Wilkinson
Download or read book Techniques in Lipid and Membrane Biochemistry written by M. J. Wilkinson and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advanced Techniques in Biological Electron Microscopy by : J.K. Koehler
Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Book Synopsis Techniques in Lipid and Membrane Biochemistry, Part 1-2 by : M.J. Wilkinson
Download or read book Techniques in Lipid and Membrane Biochemistry, Part 1-2 written by M.J. Wilkinson and published by . This book was released on 1982 with total page 25 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Freeze Fracture Study of Membranes Involved in Transport by : Graeme Peter Dempsey
Download or read book A Freeze Fracture Study of Membranes Involved in Transport written by Graeme Peter Dempsey and published by . This book was released on 1971 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Freeze-fracture Replication of Biological Tissues by : C. Stolinski
Download or read book Freeze-fracture Replication of Biological Tissues written by C. Stolinski and published by . This book was released on 1975 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:
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