Formalin Fixed Paraffin Embedded Tissues

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General Methods in Biomarker Research and their Applications

Author : Victor R. Preedy
Publisher : Springer
ISBN 13 : 9789400776951
Total Pages : 0 pages
Book Rating : 4.7/5 (769 download)

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Book Synopsis General Methods in Biomarker Research and their Applications by : Victor R. Preedy

Download or read book General Methods in Biomarker Research and their Applications written by Victor R. Preedy and published by Springer. This book was released on 2015-08-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade there has been a major sea change in the way disease is diagnosed and investigated due to the advent of high throughput technologies, such as microarrays, lab on a chip, proteomics, genomics, lipomics, metabolomics etc. These advances have enabled the discovery of new and novel markers of disease relating to autoimmune disorders, cancers, endocrine diseases, genetic disorders, sensory damage, intestinal diseases etc. In many instances these developments have gone hand in hand with the discovery of biomarkers elucidated via traditional or conventional methods, such as histopathology or clinical biochemistry. Together with microprocessor-based data analysis, advanced statistics and bioinformatics these markers have been used to identify individuals with active disease or pathology as well as those who are refractory or have distinguishing pathologies. New analytical methods that have been used to identify markers of disease and is suggested that there may be as many as 40 different platforms. Unfortunately techniques and methods have not been readily transferable to other disease states and sometimes diagnosis still relies on single analytes rather than a cohort of markers. There is thus a demand for a comprehensive and focused evidenced-based text and scientific literature that addresses these issues. Hence the formulation of Biomarkers in Disease. The series covers a wide number of areas including for example, nutrition, cancer, endocrinology, cardiology, addictions, immunology, birth defects, genetics and so on. The chapters are written by national or international experts and specialists.

Formalin-Fixed Paraffin-Embedded Tissues

Author : Fahd Al-Mulla
Publisher : Humana Press
ISBN 13 : 9781493961719
Total Pages : 312 pages
Book Rating : 4.9/5 (617 download)

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Book Synopsis Formalin-Fixed Paraffin-Embedded Tissues by : Fahd Al-Mulla

Download or read book Formalin-Fixed Paraffin-Embedded Tissues written by Fahd Al-Mulla and published by Humana Press. This book was released on 2016-08-23 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.

Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate

Author :
Publisher : Academic Press
ISBN 13 : 012420094X
Total Pages : 408 pages
Book Rating : 4.1/5 (242 download)

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Book Synopsis Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate by :

Download or read book Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate written by and published by Academic Press. This book was released on 2013-10-30 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate

Searching for Small Molecules in Formalin-fixed Paraffin-embedded Tissue Surrogates

Author : Charis Lam
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (133 download)

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Book Synopsis Searching for Small Molecules in Formalin-fixed Paraffin-embedded Tissue Surrogates by : Charis Lam

Download or read book Searching for Small Molecules in Formalin-fixed Paraffin-embedded Tissue Surrogates written by Charis Lam and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Formalin-fixed paraffin-embedded (FFPE) tissues are housed in the millions within archival banks. They may provide a treasure trove of samples for exploring the small-molecule basis of disease-if they still retain their small-molecule metabolites. Fixation and embedding is chemically harsh, and many metabolites may not survive the process. In this thesis, I use tissue surrogates as a tissue mimic to measure small-molecule recovery from FFPE samples. Using six model small-molecule analytes, I show that much analyte is lost-in whole or in part-during sample processing, and that the extent of loss is compound dependent. Further, I present preliminary data suggesting that tissue-surrogate models produce unbiased testosterone-extraction measurements when used to represent real tissue. These results add to our knowledge about the data quality to be expected from FFPE samples, and thus give future researchers more information when choosing whether to use such specimens in their work.

RNA Isolation and Characterization Protocols

Author : Ralph Rapley
Publisher : Humana Press
ISBN 13 : 9781489942500
Total Pages : 264 pages
Book Rating : 4.9/5 (425 download)

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Book Synopsis RNA Isolation and Characterization Protocols by : Ralph Rapley

Download or read book RNA Isolation and Characterization Protocols written by Ralph Rapley and published by Humana Press. This book was released on 2013-08-18 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.

The Applicability of Formalin-Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis

Author : RL. Romero
Publisher :
ISBN 13 :
Total Pages : 7 pages
Book Rating : 4.:/5 (125 download)

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Book Synopsis The Applicability of Formalin-Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis by : RL. Romero

Download or read book The Applicability of Formalin-Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis written by RL. Romero and published by . This book was released on 1997 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Guidelines for Molecular Analysis in Archive Tissues

Author : Giorgio Stanta
Publisher : Springer Science & Business Media
ISBN 13 : 3642178901
Total Pages : 301 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Guidelines for Molecular Analysis in Archive Tissues by : Giorgio Stanta

Download or read book Guidelines for Molecular Analysis in Archive Tissues written by Giorgio Stanta and published by Springer Science & Business Media. This book was released on 2011-06-06 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.

The Applicability of Formalin Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis

Author : Renee L. Romero
Publisher :
ISBN 13 :
Total Pages : 70 pages
Book Rating : 4.:/5 (399 download)

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Book Synopsis The Applicability of Formalin Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis by : Renee L. Romero

Download or read book The Applicability of Formalin Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis written by Renee L. Romero and published by . This book was released on 1994 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Nucleic Acids Extraction from Formalin-Fixed and Paraffin-Embedded Tissues

Author : Gisele R. Gouveia
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (115 download)

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Book Synopsis Nucleic Acids Extraction from Formalin-Fixed and Paraffin-Embedded Tissues by : Gisele R. Gouveia

Download or read book Nucleic Acids Extraction from Formalin-Fixed and Paraffin-Embedded Tissues written by Gisele R. Gouveia and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Formalin-fixed paraffin-embedded (FFPE) tissues are an important sample source for retrospective studies. Despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests since it fragments and chemically modifies nucleic acids, especially RNA. Although several studies describe techniques that allow extracting nucleic acids from FFPE tissues, so far there is no consensus in the literature about the best protocol to be used in this type of material. Thus, the current chapter aims to describe the factors affecting the FFPE tissue nucleic acid extracting process, compare the available protocols and to describe the modifications developed by our group in some protocols. Such modifications enable nucleic acids obtainment in satisfactory quantity and quality for molecular biology studies.

Biobanking

Author : William H. Yong
Publisher : Humana Press
ISBN 13 : 9781493989331
Total Pages : 470 pages
Book Rating : 4.9/5 (893 download)

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Book Synopsis Biobanking by : William H. Yong

Download or read book Biobanking written by William H. Yong and published by Humana Press. This book was released on 2018-12-14 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.

Nucleic Acids

Author : Marcelo Larramendy
Publisher : BoD – Books on Demand
ISBN 13 : 9535122649
Total Pages : 222 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Nucleic Acids by : Marcelo Larramendy

Download or read book Nucleic Acids written by Marcelo Larramendy and published by BoD – Books on Demand. This book was released on 2016-03-16 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book, "Nucleic Acids - From Basic Aspects to Laboratory Tools", contains a series of chapters that highlight the development and status of the various aspects of the nucleic acids related to DNA chemistry and biology and the molecular application of these small DNA molecules and related synthetic analogues within biological systems. Furthermore, it is hoped that the information in the present book will be of value to those directly engaged in the handling and use of nucleic acids, and that this book will continue to meet the expectations and needs of all who are interested in the different fascinating aspects of molecular biology.

Immunostaining of Formalin-fixed, Paraffin-embedded Tissues for Malignant Lymphomas

Author : Y. C. Lim
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (968 download)

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Book Synopsis Immunostaining of Formalin-fixed, Paraffin-embedded Tissues for Malignant Lymphomas by : Y. C. Lim

Download or read book Immunostaining of Formalin-fixed, Paraffin-embedded Tissues for Malignant Lymphomas written by Y. C. Lim and published by . This book was released on 1992 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Antigen Retrieval Techniques

Author : Shan-Rong Shi
Publisher : Eaton Publishing Company/Biotechniques Books
ISBN 13 :
Total Pages : 388 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Antigen Retrieval Techniques by : Shan-Rong Shi

Download or read book Antigen Retrieval Techniques written by Shan-Rong Shi and published by Eaton Publishing Company/Biotechniques Books. This book was released on 2000 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Qualitative Comparison of Fresh Frozen and Formalin Fixed Paraffin-embedded Tissue Samples - Impact of Fixation and Storage Duration on the Quality of DNA and RNA Extracts

Download Qualitative Comparison of Fresh Frozen and Formalin Fixed Paraffin-embedded Tissue Samples - Impact of Fixation and Storage Duration on the Quality of DNA and RNA Extracts PDF Online Free

Author : Carlota Lucena Porcel
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (119 download)

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Book Synopsis Qualitative Comparison of Fresh Frozen and Formalin Fixed Paraffin-embedded Tissue Samples - Impact of Fixation and Storage Duration on the Quality of DNA and RNA Extracts by : Carlota Lucena Porcel

Download or read book Qualitative Comparison of Fresh Frozen and Formalin Fixed Paraffin-embedded Tissue Samples - Impact of Fixation and Storage Duration on the Quality of DNA and RNA Extracts written by Carlota Lucena Porcel and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Surgical Pathology

Author : Ahmad Altaleb
Publisher : Springer Nature
ISBN 13 : 3030536904
Total Pages : 152 pages
Book Rating : 4.0/5 (35 download)

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Book Synopsis Surgical Pathology by : Ahmad Altaleb

Download or read book Surgical Pathology written by Ahmad Altaleb and published by Springer Nature. This book was released on 2020-10-19 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a practical and clinically oriented guide to the concepts of pathologic diagnosis of surgical specimens. Concise and highly illustrated chapters cover essential information required within patient management. Telepathology, ancillary techniques, and surgical oncology concepts are also examined. Surgical Pathology: A Practical Guide aims to bridge the knowledge gap between surgeons and pathologists to promote mutual understanding and a better working relationship. This book is relevant to general surgeons, and surgical oncologists, whether in training or in practice. It also serves as an introduction for first-year pathology residents, and medical students interested in surgical pathology.

Recovery of Pure Cell Populations from Archived Formalin-fixed Paraffin-embedded Tissue for DNA and RNA Analysis

Author : Kristina Lorena Seiler
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (115 download)

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Book Synopsis Recovery of Pure Cell Populations from Archived Formalin-fixed Paraffin-embedded Tissue for DNA and RNA Analysis by : Kristina Lorena Seiler

Download or read book Recovery of Pure Cell Populations from Archived Formalin-fixed Paraffin-embedded Tissue for DNA and RNA Analysis written by Kristina Lorena Seiler and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

PCR Protocols

Author : John M. S. Bartlett
Publisher : Springer Science & Business Media
ISBN 13 : 1592593844
Total Pages : 1083 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis PCR Protocols by : John M. S. Bartlett

Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.