FISH Technology

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Author :
Publisher : Elsevier
ISBN 13 : 9783540672760
Total Pages : 508 pages
Book Rating : 4.6/5 (727 download)

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Book Synopsis FISH Technology by : Bernd W. Rautenstrauß

Download or read book FISH Technology written by Bernd W. Rautenstrauß and published by Elsevier. This book was released on 2002-02-14 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.

Fluorescence In Situ Hybridization (FISH) - Application Guide

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 3540705813
Total Pages : 447 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Fluorescence In Situ Hybridization (FISH) - Application Guide by : Thomas Liehr

Download or read book Fluorescence In Situ Hybridization (FISH) - Application Guide written by Thomas Liehr and published by Springer Science & Business Media. This book was released on 2008-11-26 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.

Fluorescence In-Situ Hybridization (FISH) for Microbial Cells

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Author :
Publisher :
ISBN 13 : 9781071611173
Total Pages : pages
Book Rating : 4.6/5 (111 download)

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Book Synopsis Fluorescence In-Situ Hybridization (FISH) for Microbial Cells by : Nuno F. Azevedo

Download or read book Fluorescence In-Situ Hybridization (FISH) for Microbial Cells written by Nuno F. Azevedo and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

The AGT Cytogenetics Laboratory Manual

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 1119061229
Total Pages : 1216 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis The AGT Cytogenetics Laboratory Manual by : Marilyn S. Arsham

Download or read book The AGT Cytogenetics Laboratory Manual written by Marilyn S. Arsham and published by John Wiley & Sons. This book was released on 2017-04-24 with total page 1216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.

Basic and Advanced Laboratory Techniques in Histopathology and Cytology

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Author :
Publisher : Springer Nature
ISBN 13 : 9811966168
Total Pages : 350 pages
Book Rating : 4.8/5 (119 download)

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Book Synopsis Basic and Advanced Laboratory Techniques in Histopathology and Cytology by : Pranab Dey

Download or read book Basic and Advanced Laboratory Techniques in Histopathology and Cytology written by Pranab Dey and published by Springer Nature. This book was released on 2023-01-01 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.

Chromosome Structure and Aberrations

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Author :
Publisher : Springer
ISBN 13 : 8132236734
Total Pages : 377 pages
Book Rating : 4.1/5 (322 download)

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Book Synopsis Chromosome Structure and Aberrations by : Tariq Ahmad Bhat

Download or read book Chromosome Structure and Aberrations written by Tariq Ahmad Bhat and published by Springer. This book was released on 2017-02-08 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compilation of various chapters contributed by a group of leading researchers from different countries and covering up to date information based on published reports and personal experience of authors in the field of cytogenetics. Beginning with the introduction of chromosome, the subsequent chapters on organization of genetic material, karyotype evolution, structural and numerical variations in chromosomes, B-chromosomes and chromosomal aberrations provide an in-depth knowledge and easy understanding of the subject matter. A special feature of the book is the inclusion of a series of chapters on various types of chromosomal aberrations and their impact on breeding behaviour and crop improvement. The possible mechanism, their consequences and role in genetic analysis has been emphasized in these chapters. A few chapters have also been dedicated on various techniques routinely used in the laboratory by students and researchers. Each chapter ends with an extensive bibliography so that the students and researchers may find it relevant to consult more literature on the subject than a book of this size can offer. The book is intended to fulfill the needs of undergraduate and post graduate students of botany, zoology and agriculture besides, teachers and researchers engaged in the field of genetics, cytogenetics, and molecular genetics. In general the readers will find each chapter of the book informative and easy to understand.

Modern Techniques in Cytopathology

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Author :
Publisher : Karger Medical and Scientific Publishers
ISBN 13 : 3318065765
Total Pages : 120 pages
Book Rating : 4.3/5 (18 download)

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Book Synopsis Modern Techniques in Cytopathology by : M.M. Bui

Download or read book Modern Techniques in Cytopathology written by M.M. Bui and published by Karger Medical and Scientific Publishers. This book was released on 2020-01-13 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the era of precision medicine, physicians are increasingly in need of more definitive diagnostic, prognostic, and predictive information derived from small biopsy specimens such as cytology samples in order to guide effective patient care. Cytopathology is well poised to meet this challenge. Whilst the traditional cytomorphologic component of cytology practice is still valid, enormous advances have been made in the field of cytopathology thanks to transformative technology and innovative individuals that have augmented the cytologists' ability to meet the demands of modern medicine. The purpose of this book is to describe, illustrate, and review many of the most recent developments regarding modern techniques employed in cytopathology. This latest monograph is intended for all cytologists including cytopathologists, cytotechnologists, cytology lab assistants, trainees, research scientists, and anyone who is interested in the field of cytopathology. We have invited pioneering experts in their respective fields to author these chapters. This book is not only the culmination of their groundbreaking work and effort but also presents a critical review of the current literature. We have attempted to provide readers with an informative and comprehensive aid so that they may better appreciate how emerging technology has been applied to cytology. Each chapter in this book presents a stand-alone contemporary review of emerging topics in cytopathology. We hope that you will find this monograph thought-provoking and a valuable reference for your practice.

Deep Sternal Wound Infections

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Author :
Publisher : Springer
ISBN 13 : 3662497662
Total Pages : 139 pages
Book Rating : 4.6/5 (624 download)

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Book Synopsis Deep Sternal Wound Infections by : Raymund E. Horch

Download or read book Deep Sternal Wound Infections written by Raymund E. Horch and published by Springer. This book was released on 2017-01-19 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This concise and practical handbook covers the basics of pathophysiology, diagnosis, interdisciplinary surgical management, prevention and rehabilitation of patients with deep sternal wound infections and sternal osteomyelitis. All relevant aspects and surgical procedures are explained in an easily understandable way. Additionally special approaches and preventive measures are highlighted with regard to the perioperative handling as well as the rehabilitation possibilities. Through concise texts with numerous illustrations, the book is ideal for the practice and as a supplement to further studies. This book is suitable for all specialists who are involved into the treatment and diagnosis of sternal wound infections, particularly cardio-thoracis, thoracic, plastic, vascular surgeons, cardiologists, radiologists, and rehabilitation physicians.

The Principles of Clinical Cytogenetics

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Author :
Publisher : Springer
ISBN 13 :
Total Pages : 580 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis The Principles of Clinical Cytogenetics by : Steven L. Gersen

Download or read book The Principles of Clinical Cytogenetics written by Steven L. Gersen and published by Springer. This book was released on 1999-03-17 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enlightening and accessible, The Principles of Clinical Cytogenetics constitutes an indispensable reference for today's physicians who depend on the cytogenetics laboratory for the diagnosis of their patients.

FISH Handbook for Biological Wastewater Treatment

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Author :
Publisher : IWA Publishing
ISBN 13 : 1843392313
Total Pages : 141 pages
Book Rating : 4.8/5 (433 download)

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Book Synopsis FISH Handbook for Biological Wastewater Treatment by : Per Halkjaer Nielsen

Download or read book FISH Handbook for Biological Wastewater Treatment written by Per Halkjaer Nielsen and published by IWA Publishing. This book was released on 2009-07-14 with total page 141 pages. Available in PDF, EPUB and Kindle. Book excerpt: The FISH Handbook for Biological Wastewater Treatment provides all the required information for the user to be able to identify and quantify important microorganisms in activated sludge and biofilms by using fluorescence in situ hybridization (FISH) and epifluorescence microscopy. It has for some years been clear that most microorganisms in biological wastewater systems cannot be reliably identified and quantified by conventional microscopy or by traditional culture-dependent methods such as plate counts. Therefore, molecular biological methods are vital and must be introduced instead of, or in addition to, conventional methods. At present, FISH is the most widely used and best tested of these methods. This handbook presents all relevant information from the literature and, based on the extensive experience of the authors, advice and recommendations are given for reliable FISH identification and quantification. The overall purpose of the book is to help scientists, consultants, students, and plant operators to get an overview of important microorganisms in biological wastewater treatment and to explain how FISH can be used for detecting and quantifying these microbes. A proper and reliable identification of dominant microorganisms is of great importance for research and new developments in the wastewater treatment industry, and it is important for optimization and troubleshooting of operational problems in present wastewater treatment plants. The book encompasses an overview of dominant microorganisms present in the wastewater treatment systems, which oligonucleotide probes (gene probes) to select for detection of these microbes by FISH, how to perform FISH (detailed protocols), how to quantify the microbes, and how to solve common problems of FISH. The book addresses several functional groups: nitrifiers, denitrifiers, polyphosphate-accumulating organisms, glycogen-accumulating organisms, bacteria involved in hydrolysis and fermentation, filamentous bacteria from bulking sludge, and scum-forming bacteria. A comprehensive collection of FISH-images showing dominant representatives of these groups helps readers to use FISH in the context of wastewater treatment.

Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues

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Author :
Publisher : IARC
ISBN 13 : 9789283224112
Total Pages : 360 pages
Book Rating : 4.2/5 (241 download)

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Book Synopsis Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues by : World Health Organization

Download or read book Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues written by World Health Organization and published by IARC. This book was released on 2001 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third volume in the new World Health Organization series on histological and genetic typing of tumours. Tumours of the haematopoietic and lymphoid tissues are covered. This was a collaborative project of the European Association for Haematolpathology and the Society for Haematopathology and others. The WHO classification is based on the principles defined in the Revised European-American Classification of Lymphoid Neoplasms (REAL) classification. Over 50 pathologists from around the world were involved in the project and proponents of all major lymphoma and leukaemia classifications have agreed to accept the WHO as the standard classification of haematological malignancies. So this classification represents the first true world wide concensus of haematologic malignancies. Colour photographs, magnetic resonance and ultrasound images and CT scans are included.

ISCN 2013

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Publisher : Karger Medical and Scientific Publishers
ISBN 13 : 3318022535
Total Pages : 148 pages
Book Rating : 4.3/5 (18 download)

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Book Synopsis ISCN 2013 by : International Standing Committee on Human Cytogenetic Nomenclature

Download or read book ISCN 2013 written by International Standing Committee on Human Cytogenetic Nomenclature and published by Karger Medical and Scientific Publishers. This book was released on 2013 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication extends the now classic system of human cytogenetic nomenclature prepared by an expert committee and published in collaboration with Cytogenetic and Genome Research' since 1963. Revised and finalized by the ISCN Committee and its advisors at a meeting in Seattle, Wash., in April 2012, the ISCN 2013 updates, revises and incorporates all previous human cytogenetic nomenclature recommendations into one systematically organized publication that supersedes all previous ISCN recommendations. There are several new features in ISCN 2013: an update of the microarray nomenclature, many more illustrative examples of uses of nomenclature in all sections some definitions including chromothripsis and duplication a new chapter for nomenclature that can be used for any region-specific assay. The ISCN 2013 is an indispensable reference volume for human cytogeneticists, technicians and students for the interpretation and communication of human cytogenetic nomenclature.

In Situ Hybridization Methods

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Author :
Publisher : Humana
ISBN 13 : 9781493944637
Total Pages : 0 pages
Book Rating : 4.9/5 (446 download)

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Book Synopsis In Situ Hybridization Methods by : Giselbert Hauptmann

Download or read book In Situ Hybridization Methods written by Giselbert Hauptmann and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

Molecular Structure of Nucleic Acids

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Author :
Publisher : Ardent Media
ISBN 13 :
Total Pages : 8 pages
Book Rating : 4./5 ( download)

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Book Synopsis Molecular Structure of Nucleic Acids by :

Download or read book Molecular Structure of Nucleic Acids written by and published by Ardent Media. This book was released on 1953 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Cytogenetic Laboratory Management

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 1119069742
Total Pages : 844 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Cytogenetic Laboratory Management by : Susan Mahler Zneimer

Download or read book Cytogenetic Laboratory Management written by Susan Mahler Zneimer and published by John Wiley & Sons. This book was released on 2016-11-21 with total page 844 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetic Laboratory Management Cytogenetic Laboratory Management Chromosomal, FISH and Microarray-Based Best Practices and Procedures Cytogenetic Laboratory Management: Chromosomal, FISH and Microarray-Based Best Practices and Procedures is a practical guide that describes how to develop and implement best practice processes and procedures in the genetic laboratory setting. The text first describes good laboratory practices, including quality management, design control of tests, and FDA guidelines for laboratory-developed tests, and preclinical validation study designs. The second focus of the book is on best practices for staffing and training, including cost of testing, staffing requirements, process improvement using Six Sigma techniques, training and competency guidelines, and complete training programs for cytogenetic and molecular genetic technologists. The third part of the text provides stepwise standard operating procedures for chromosomal, FISH and microarray-based tests, including preanalytic, analytic, and postanalytic steps in testing, which are divided into categories by specimen type and test type. All three sections of the book include example worksheets, procedures, and other illustrative examples that can be downloaded from the Wiley website to be used directly without having to develop prototypes in your laboratory. Providing a wealth of information on both laboratory management and molecular and cytogenetic testing, Cytogenetic Laboratory Management will be an essential tool for laboratorians worldwide in the field of laboratory testing and genetic testing in particular. This book gives the essentials of: Developing and implementing good quality management programs in laboratories Understanding design control of tests and preclinical validation studies and reports FDA guidelines for laboratory-developed tests Use of reagents, instruments, and equipment Cost of testing assessment and process improvement using Six Sigma methodology Staffing training and competency objectives Complete training programs for molecular and cytogenetic technologists Standard operating procedures for all components of chromosomal analysis, FISH, and microarray testing of different specimen types This volume is a companion to Cytogenetic Abnormalities: Chromosomal, FISH and Microarray-Based Clinical Reporting. The combined volumes give an expansive approach to performing, reporting, and interpreting cytogenetic laboratory testing and the necessary management practices, staff and testing requirements.

The Functional Nucleus

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Author :
Publisher : Springer
ISBN 13 : 3319388827
Total Pages : 501 pages
Book Rating : 4.3/5 (193 download)

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Book Synopsis The Functional Nucleus by : David P. Bazett-Jones

Download or read book The Functional Nucleus written by David P. Bazett-Jones and published by Springer. This book was released on 2016-10-17 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an in-depth overview on nuclear structure and function. It clearly shows that the epigenome and the three-dimensional organization of the nucleus are not independent properties. The intimate relationship between the location and the epigenetic modifications of gene loci is highlighted. Finally, it shows that the complex three-dimensional organization of the nucleus is not just of academic interest: The structure, composition and function of virtually all of the sub-nuclear compartments identified so far can be implicated to a list of human genetic diseases. Hence, a detailed elucidation of how these domains are assembled and function will provide new opportunities for therapeutic intervention in clinical practice.

Fish Cytogenetic Techniques

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Author :
Publisher : CRC Press
ISBN 13 : 1482211998
Total Pages : 216 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Fish Cytogenetic Techniques by : Catherine Ozouf-Costaz

Download or read book Fish Cytogenetic Techniques written by Catherine Ozouf-Costaz and published by CRC Press. This book was released on 2015-06-17 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in fish cytogenetics have enhanced the interest in chromosome analysis in both fundamental (systematics and comparative genomics among fishes and other vertebrate groups) and applied (aquaculture, conservation and response to pollutants, whole genome sequencing of model fish species) research. Although the genomic material, the chro