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Experimental Process Biotechnology Protocols
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Book Synopsis Experimental Process Biotechnology Protocols by : Satya Narayan Mukhopadhyay
Download or read book Experimental Process Biotechnology Protocols written by Satya Narayan Mukhopadhyay and published by . This book was released on 2007-06-01 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental Process Biotechnology is one of the basic requirements for Biochemical Engineering, Bioprocess Engineering and Biotechnology. Theoretical basis of this course encampusing analytical bio-chemistry, microbiology, cell biology,biochemical and chemical engineering sciences, general and applied molecular biology, plant and animal cell culture engineering etc. is now well founded. This laboratory manual containing protocols is prepared on the basis of my personal learning exposures at Jadavpur University, Kolkata, experiences and supervision of laboratory work in microbial and enzyme engineering and technology conducted at Biochemical Engineering Research Centre, Deptt of Biochemical Engineering & Biotechnology, IIT Delhi, Fermentation technology laboratory, Osaka University, Japan and other places.
Book Synopsis Molecular Biology and Biotechnology by : M. P. Bansal
Download or read book Molecular Biology and Biotechnology written by M. P. Bansal and published by The Energy and Resources Institute (TERI). This book was released on 2013-01-01 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology and Biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.
Author :S. N. Mukhopadhyay Publisher :The Energy and Resources Institute (TERI) ISBN 13 :8179933075 Total Pages :622 pages Book Rating :4.1/5 (799 download)
Book Synopsis Process Biotechnology by : S. N. Mukhopadhyay
Download or read book Process Biotechnology written by S. N. Mukhopadhyay and published by The Energy and Resources Institute (TERI). This book was released on 2012-01-01 with total page 622 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid progress has been made in the discipline of biochemical engineering and biotechnology for bioprocess development during the last 50 years. Process Biotechnology: theory and practice has been written with the consideration that tutorial practice is as important as understanding the subject theoretically. This book is an introductory tutorial book involving multidisciplinary principles. Principal innovations that have been made in biosystem-related developments have been emphasized through tutorials in this book. The first few chapters cover theoretical aspects of biochemical and chemical engineering concerns in biotechnological advances in a concise manner. The rest have been dedicated to the tutorial aspects of this multidisciplinary subject. This book covers biological, ecological, chemical, and biochemical engineering topics related to the subject. It provides much needed theory-based solved numerical problems for practice in quantitative evaluation of various parameters relevant to process biotechnology. It will be useful for students who would like to further their careers as biotechnologists and can be used as a self-study text for practicing engineers, biotechnologists, microbiologists, and scientists involved in bioprocessing research and other related fields.
Book Synopsis Experimental Biotechnology by : Sunita Dutta
Download or read book Experimental Biotechnology written by Sunita Dutta and published by New India Publishing. This book was released on 2011-06-03 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.
Book Synopsis Process Biotechnology Fundamentals by : Satya Narayan Mukhopadhyay
Download or read book Process Biotechnology Fundamentals written by Satya Narayan Mukhopadhyay and published by MV Learning. This book was released on 2019-06-30 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Newer intellectual challenges for process biotechnology (PB) have emerged with impressive recent progress of gene-concerned and nano-systemic biosystems and the parallel developments in the related instruments/equipment/machines for analytical methodologies. These progresses have convinced universities/institutes and affiliatedcolleges that biotechnology (BT)/PB is one of the key areas for RD and education in biochemical engineering/chemical engineering and their related interdisciplinary highereducation areas for human resource development (HRD). This book has the following unique features: - Integration of academic and practical bioprocessing principles towards process biotechnology development. - Research generated data never before published in a single collated text. - Detailed aspects of oxygen mass transfer in biological systems. - Ways to control undesirable microorganisms. - Provides scope for progressive development in scale up of industrialbioprocessing system. - Systematic design engineering calculations of bioprocessing systems. - PCR instrumental analysis: theory, engineering and technology. - Heat and mass transfer concerns in rDNA bioreactors. - Innovative microbial microfermentation equipment and aspect. - Nanosystemic PB concerns in bioelectricity generation in biofuel cell. - Nanosight instrument and use in analytical biosystems. - Enhancing scopes of BTBE in PB and education. - Abundance of illustrative tables, figures and mathematical relations. - Numerous mathematical problems and multiple choice questions.
Book Synopsis Advanced Process Biotechnology by : Satya Narayan Mukhopadhyay
Download or read book Advanced Process Biotechnology written by Satya Narayan Mukhopadhyay and published by MV Learning. This book was released on 2015-08-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Process Biotechnology practice has advanced substantially in more recent years. The advancements have taken place not only with microbes or microbial systems but also with mammalian, animal and plant cell culture process biotechnology systems. For protecting these advancements by contributors various concerns of patenting has been presented in brief for this field. Unique major features of the book include, Patenting concerns of life forms for process biotechnology, Cell redesigning methods and associated concerns, Impressive progress coverage of advanced topics, Thermophilic bioprocessing, Containment requirements in process biotechnology using GMO, Apoptotic phenomena in bioreactor for bioprocessing, Highlights of technical challenges for recovery/separation of products, In vivo- Ex vivo membrane phenomena in cellular process biotechnology, Application of ANN in bioprocess control, Extractive bioconversion, Advances in Biosensors, Importances of cross linking agents in stabilizing biochemicals, Miscellaneous industrial bioproducts etc.
Download or read book Cell Biotechnology written by R. Tyagi and published by Discovery Publishing House. This book was released on 2005 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contents: Morphology of Cell, Chemical Environment of Cell, Centrifugation, Primary Cell Line, Plant Cell Culture, Plant Cell Culture, Formation of Protoplasts, Maintenance of a Cell Line, Isolation of Chloroplasts, Isolation of Genomic DNA, Isolation of Chloroplast DNA, Isolation of Mitochondria, Principle of Chromatography, Spectrophotometry, Cytological Techniques, Histological Stains, Histochemical Stains, Specific Staining for Various Tissues, The Microscopes.
Book Synopsis A Practical Guide to Pharmacological Biotechnology by : Jayanta Kumar Patra
Download or read book A Practical Guide to Pharmacological Biotechnology written by Jayanta Kumar Patra and published by Springer. This book was released on 2019-03-25 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pharmacological biotechnology is applied to and used to study drug development, working mechanisms, diagnosis, and therapies. This textbook covers the whole range of experiments related to pharmacology. It also contains basic laboratory safety guidelines along with the basic calculations and formulas used in a laboratory. Each chapter starts with an introduction/theory into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting, calculations and possible questions for examination. The target group is researchers who are studying pharmacological biotechnology in the laboratory.
Book Synopsis Practical Biotechnology by : H. N. Thatoi
Download or read book Practical Biotechnology written by H. N. Thatoi and published by . This book was released on 2017-09-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces the different tools and methods of molecular biology from both a theoretical and practical perspective. Discusses the principles and procedures, their potential and drawbacks, involved in experiments in laboratories. Provides information on safety guidelines, ethical issues, genetic engineering work and laboratory set-ups. The books is aimed at advanced students, as well as research scientists and technicians.
Book Synopsis Academia to Biotechnology by : Jeffrey M Gimble
Download or read book Academia to Biotechnology written by Jeffrey M Gimble and published by Elsevier. This book was released on 2004-10-08 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: Academia to Biotechnology deals with both the abstract and practical aspects of moving from a univerisity laboratory to a position in the biotech industry. Each chapter lists common and unique features to evaluate breaking down complex decisions into manageable elements. Several sections provide "how to" guides for the preparation of manuscripts, patents, grants, and internal company documents. Written by an experienced academician and successful biotechnology entrepreneur Reviews the basic tools taught in a traditional university Identifies new ways these these tools will be used in the corporate world Details the 'nuts and bolts' necessary to negotiate a successful position in the biotech industry
Book Synopsis Current Protocols in Molecular Biology by :
Download or read book Current Protocols in Molecular Biology written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Experimental Protocols in Biotechnology by : Neelima Gupta
Download or read book Experimental Protocols in Biotechnology written by Neelima Gupta and published by . This book was released on 2020 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses. .
Book Synopsis Current Protocols Essential Laboratory Techniques by : Sean R. Gallagher
Download or read book Current Protocols Essential Laboratory Techniques written by Sean R. Gallagher and published by John Wiley & Sons. This book was released on 2012-03-19 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Book Synopsis Microbial Interventions in Agriculture and Environment by : Dhananjaya Pratap Singh
Download or read book Microbial Interventions in Agriculture and Environment written by Dhananjaya Pratap Singh and published by Springer Nature. This book was released on 2019-11-27 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial communities and their functions play a crucial role in the management of ecological, environmental and agricultural health on the Earth. Microorganisms are the key identified players for plant growth promotion, plant immunization, disease suppression, induced resistance and tolerance against stresses as the indicative parameters of improved crop productivity and sustainable soil health. Beneficial belowground microbial interactions with the rhizosphere help plants mitigate drought and salinity stresses and alleviate water stresses under the unfavorable environmental conditions in the native soils. Microorganisms that are inhabitants of such environmental conditions have potential solutions for them. There are potential microbial communities that can degrade xenobiotic compounds, pesticides and toxic industrial chemicals and help remediate even heavy metals, and thus they find enormous applications in environmental remediation. Microbes have developed intrinsic metabolic capabilities with specific metabolic networks while inhabiting under specific conditions for many generations and, so play a crucial role. The book Microbial Interventions in Agriculture and Environment is an effort to compile and present a great volume of authentic, high-quality, socially-viable, practical and implementable research and technological work on microbial implications. The whole content of the volume covers protocols, methodologies, applications, interactions, role and impact of research and development aspects on microbial interventions and technological outcomes in prospects of agricultural and environmental domain including crop production, plan-soil health management, food & nutrition, nutrient recycling, land reclamation, clean water systems and agro-waste management, biodegradation & bioremediation, biomass to bioenergy, sanitation and rural livelihood security. The covered topics and sub-topics of the microbial domain have high implications for the targeted and wide readership of researchers, students, faculty and scientists working on these areas along with the agri-activists, policymakers, environmentalists, advisors etc. in the Government, industries and non-government level for reference and knowledge generation.
Book Synopsis Downstream Industrial Biotechnology by : Michael C. Flickinger
Download or read book Downstream Industrial Biotechnology written by Michael C. Flickinger and published by John Wiley & Sons. This book was released on 2013-07-17 with total page 884 pages. Available in PDF, EPUB and Kindle. Book excerpt: DOWNSTREAM INDUSTRIAL BIOTECHNOLOGY An affordable, easily accessible desk reference on biomanufacturing, focused on downstream recovery and purification Advances in the fundamental knowledge surrounding biotechnology, novel materials, and advanced engineering approaches continue to be translated into bioprocesses that bring new products to market at a significantly faster pace than most other industries. Industrial scale biotechnology and new manufacturing methods are revolutionizing medicine, environmental monitoring and remediation, consumer products, food production, agriculture, and forestry, and continue to be a major area of research. The downstream stage in industrial biotechnology refers to recovery, isolation, and purification of the microbial products from cell debris, processing medium and contaminating biomolecules from the upstream process into a finished product such as biopharmaceuticals and vaccines. Downstream process design has the greatest impact on overall biomanufacturing cost because not only does the biochemistry of different products ( e.g., peptides, proteins, hormones, antibiotics, and complex antigens) dictate different methods for the isolation and purification of these products, but contaminating byproducts can also reduce overall process yield, and may have serious consequences on clinical safety and efficacy. Therefore downstream separation scientists and engineers are continually seeking to eliminate, or combine, unit operations to minimize the number of process steps in order to maximize product recovery at a specified concentration and purity. Based on Wiley’s Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, this volume features fifty articles that provide information on down- stream recovery of cells and protein capture; process development and facility design; equipment; PAT in downstream processes; downstream cGMP operations; and regulatory compliance. It covers: Cell wall disruption and lysis Cell recovery by centrifugation and filtration Large-scale protein chromatography Scale down of biopharmaceutical purification operations Lipopolysaccharide removal Porous media in biotechnology Equipment used in industrial protein purification Affinity chromatography Antibody purification, monoclonal and polyclonal Protein aggregation, precipitation and crystallization Freeze-drying of biopharmaceuticals Biopharmaceutical facility design and validation Pharmaceutical bioburden testing Regulatory requirements Ideal for graduate and advanced undergraduate courses on biomanufacturing, biochemical engineering, biopharmaceutical facility design, biochemistry, industrial microbiology, gene expression technology, and cell culture technology, Downstream Industrial Biotechnology is also a highly recommended resource for industry professionals and libraries.
Book Synopsis Cell Biology Protocols by : J. Robin Harris
Download or read book Cell Biology Protocols written by J. Robin Harris and published by Wiley. This book was released on 2012-01-18 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology. This book will be of interest to students and more experienced cell biologists, as well as molecular biologists and those working in genomics and proteomics who are looking for cellular techniques to validate their findings within intact cells.
Book Synopsis Biochemical Engineering by : Debabrata Das
Download or read book Biochemical Engineering written by Debabrata Das and published by CRC Press. This book was released on 2020-12-30 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical engineering mostly deals with the most complicated life systems as compared with chemical engineering. A fermenter is the heart of biochemical processes. It is essential to operate a system properly. A description of enzymatic reaction kinetics is followed by cell growth kinetics to determine several kinetic parameters. Operations and analyses of several biochemical processes are included to determine their special. The book also covers the determination of several operational parameters, such as volumetric mass transfer coefficient, mixing time, death rate constant, chemical oxygen demand, and heat of combustion. This book provides a novel description of the experimental protocol to find out several operational parameters of biochemical processes. A comprehensive collection of numerous experiments based on fundamentals, it focuses on the determination of not only the characteristics of raw materials but also other essential parameters required for the operation of biochemical processes. It also emphasizes the applicability of the analysis to various processes. Equipped with illustrative diagrams, neat flowcharts, and exhaustive tables, the book is ideal for young researchers, teachers, and scientists working towards developing a solid understanding of the experimental aspects of biochemical engineering.
