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Evaluation Of Electric Cell Substrate Impedance Sensing Ecis For The Detection Of Biological And Chemical Toxins
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Book Synopsis Evaluation of Electric Cell-substrate Impedance Sensing (ECIS) for the Detection of Biological and Chemical Toxins by : Bhavana Mohanraj
Download or read book Evaluation of Electric Cell-substrate Impedance Sensing (ECIS) for the Detection of Biological and Chemical Toxins written by Bhavana Mohanraj and published by . This book was released on 2010 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Electric Cell-Substrate Impedance Sensing and Cancer Metastasis by : Wen G. Jiang
Download or read book Electric Cell-Substrate Impedance Sensing and Cancer Metastasis written by Wen G. Jiang and published by Springer Science & Business Media. This book was released on 2012-09-14 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell based impedance sensing is becoming a new biophysical and cellular technology in cell based analyses. The technology has been used in investigation of cellular growth and death, cell adhesion and migration, cell invasion and cell-cell interactions, cell toxicity, angiogenesis, cell permeability, signal transduction and cellular behaviour under flow conditions. It is a probe free, highly sensitive, and versatile technology platform. Recent development in the technology has also allowed high throughput, automated analyses. It has been widely explored in chemistry, toxicity, cell biology, cancer biology, and other areas of chemistry, medicinal chemistry, life and medical science. Written by experts in the area of cell impedance sensing, including the Nobel Laureate Dr Ivar Giaever, this books covers the background of electric cell-substrate impedance sensing, their applications in cell based investigations, particularly in the area of cancer biology. This book is the first on this technology platform and will be a highly useful reference for molecular and cell biologists, cancer biologists, chemists and biochemists, clinical researchers who work in the areas of cell biology, molecular biology, toxicology, pharmaceutical industry, life science and medical research.
Book Synopsis The Modeling, Design, Fabrication, and Application of Biosensor Based on Electric Cell-Substrate Impedance Sensing (ECIS) Technique in Environmental Monitoring by : Xudong Zhang
Download or read book The Modeling, Design, Fabrication, and Application of Biosensor Based on Electric Cell-Substrate Impedance Sensing (ECIS) Technique in Environmental Monitoring written by Xudong Zhang and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this research, the modeling, design, fabrication, and application of ECIS sensors in environmental monitoring are studied. The ECIS sensors are able to qualify the water toxicity through measuring the cell impedance. A novel mathematical model is proposed to analyze the distribution of electric potential and current of ECIS. This mathematical model is validated by experimental data and can be used to optimize the dimension of ECIS electrodes in order to satisfy environmental monitors. The detection sensitivity of ECIS sensors is analyzed by the mathematical model and experimental data. The simulated and experimental results show that ECIS sensors with smaller radius of working electrodes yield higher impedance values, which improves signal-to-noise ratio, which is more suitable in measuring the cell morphology change influenced by environments. Several ECIS sensors are used to detect the toxicant including, phenol, ammonia, nicotine, and aldicarb, and the decreasing cell impedance indicates the toxic effect. The gradient of measured impedance qualitatively indicates the concentration of toxicants in water.
Book Synopsis Using Electric Cell-substrate Impedance Sensing (ECIS) to Measure Properties of an Individual Adherent Cell by : Pahnit Seriburi
Download or read book Using Electric Cell-substrate Impedance Sensing (ECIS) to Measure Properties of an Individual Adherent Cell written by Pahnit Seriburi and published by . This book was released on 2008 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Real-time Monitoring and Quantification of Drug Induced Changes in Endothelial Cytoskeleton Filaments Using a Cellular Impedance Biosensor by :
Download or read book Real-time Monitoring and Quantification of Drug Induced Changes in Endothelial Cytoskeleton Filaments Using a Cellular Impedance Biosensor written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of electrical signals originating from biological cells and tissues have yielded a large amount of useful information over the past years. Technologies have been developed, wherein we can monitor the health of the biological material of interest using these electrical signals. Instruments for the study of living cells have historically been of significant importance for such things as basic neuroscience, cell biology, pharmaceutical screening, environmental monitoring, and toxin detection. One of the practical realizations of these methods was successfully implemented by Giaver and Keese with their Electric Cell-Substrate Impedance Sensing (ECIS) system. The purpose of this Masters Thesis is to showcase the use of a Cellular Impedance Biosensor setup based on the ECIS system in real-time monitoring of endothelial barrier function and quantify the dynamic changes of the cytoskeleton filaments induced by different drugs. The filaments of the endothelial cell cytoskeleton play a critical role in cellular micro motion and the inflammatory response. The fact that the cellular cytoskeleton is the essential force behind all the motile activities of the cell is used in drug discovery methods to design ways so as to kill tumor cells and developing cell-based therapies for different cardiovascular pathologies. Cytochalasin D was chosen to study the actin filament response as the drug specifically inhibits the polymerization of actin filaments, which play a crucial role in the cellular mechanical strength and micromotion. Nocodazole was chosen to study microtubules, which play a pivotal part in the cellular mitotic activities and locomotion. These drugs were chosen as they have extreme effects on the cytoskeleton and would be ideal to showcase the use of the biosensor in tracking the intracellular dynamics. The biosensor system provides a simple interface for monitoring the electrical activity and impedance characteristics of populations of cultured cells over extended periods. The cell - sensor interface is created as cells attach to the gold electrode surface pre-coated with fibronectin. Using these impedance measurement techniques based on a simple cellular geometric model, we have been able to successfully monitor cellular adhesion, motility, proliferation and changes in the cellular cytoskeleton induced by different drugs as mentioned above. The kinetic response of the cellular cytoskeleton to different doses of these drugs is translated as changes in the impedance measured by the biosensor setup. The data obtained from the setup were quantified by correlating them with the images obtained by confocal microscopy. A wavelet transformation algorithm is applied to the acquired data in an attempt to capture the fluctuations and compare the cellular behavior before and after the addition of drugs. Study of the disruption of these filaments by toxins and pharmacological agents holds lot of promise to provide a model for studying their role in endothelial cell biomechanics and the pathology of cardiovascular, pulmonary, and renal disease. This thesis presents a cellular impedance biosensor setup that can carry out this study in real time with high sensitivity and reproducibility. Using these impedance measurement techniques, we have been able to successfully monitor cellular adhesion, motility, proliferation of different type of cells and their response to external stimuli.
Download or read book Lymington written by and published by . This book was released on 1910 with total page 48 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanotoxicology written by Vineet Kumar and published by CRC Press. This book was released on 2018-03-12 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the application of nanotechnology in the myriad disciplines of science and engineering--from agriculture, pharmaceuticals, material science, and biotechnology to sensors, electronics, and mechanical and electrical engineering--brings benefits it also can produce serious threats to human health and the environment that must be evaluated. The unique properties of nanomaterials make them different from their bulk counterparts. In addition to such unique properties, the nanometric size of nanomaterials can invite some detrimental effects on the health and well-being of living organisms and the environment. Thus, it is important to distinguish nanomaterials with such ill effects from nanomaterials with no or minimum toxicity. Nanotoxicology: Toxicity Evaluation, Risk Assessment and Management covers issues such as the basic principles of nanotoxicity, methods used for nanotoxicity evaluation, risk assessment and its management for nanomaterial toxicity with a focus on current trends, limitations, challenges, and future directions of nanotoxicity evaluation. Various experts from different countries discuss these issues in detail in this book. This will be helpful to researchers, educators, and students who are interested in research opportunities for avoiding the environmental and health hazards of nanomaterials. This book will also be useful for industrial practitioners, policy makers, and other professionals in the fields of toxicology, medicine, pharmacology, food, drugs, and other regulatory sciences.
Book Synopsis High-Risk Pollutants in Wastewater by : Hongqiang Ren
Download or read book High-Risk Pollutants in Wastewater written by Hongqiang Ren and published by Elsevier. This book was released on 2019-11-01 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-Risk Pollutants in Wastewater presents the basic knowledge regarding the diversity, concentrations, and health and environmental impacts of HRPs in municipal wastewater. The book summarizes information on the types (e.g. heavy metals, toxic organics and pathogens) and toxicities of HRPs in wastewater. In addition, it describes ecological and health hazards arising from the living things’ direct/indirect contacts with the HRPs during their full lifecycles (generation, disposal, discharge and reuse) in wastewater or water environments. Sections cover the concepts of appropriate technology for HRP hazard/risk assessment and wastewater treatment/reuse and the issues of strategy and policy for increasing risk control coverage. Finally, the book focuses on the resolution of water quality monitoring, wastewater treatment and disposal problems in both developed and developing countries. Presents information on HRPs and their risk assessment and control technologies Provides basic knowledge regarding the diversity, concentrations, and health and environmental impacts of HRPs in municipal wastewater Summarizes information on the types (e.g. heavy metals, toxic organics and pathogens) and toxicities of HRPs in wastewater
Book Synopsis International Conference for Innovation in Biomedical Engineering and Life Sciences by : Fatimah Ibrahim
Download or read book International Conference for Innovation in Biomedical Engineering and Life Sciences written by Fatimah Ibrahim and published by Springer. This book was released on 2015-11-26 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volumes presents the proceedings of ICIBEL 2015, organized by the Centre for Innovation in Medical Engineering (CIME) under Innovative Technology Research Cluster, University of Malaya. It was held in Kuala Lumpur, Malaysia, from 6-8 December 2015. The ICIBEL 2015 conference promotes the latest researches and developments related to the integration of the Engineering technology in medical fields and life sciences. This includes the latest innovations, research trends and concerns, challenges and adopted solution in the field of medical engineering and life sciences.
Book Synopsis Quantitative Endothelial Cell Monolayer Impedance Sensing and Analysis by :
Download or read book Quantitative Endothelial Cell Monolayer Impedance Sensing and Analysis written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The electrical analysis of the biological material has been in existence since the turn of last century. A novel application of this technology to cellular monolayers was implemented by Giaever and Keese 20 years ago with their Electrical Cell-Substrate Impedance Sensing (ECIS) system. The capabilities of a real-time system for endothelial impedance measurement are of immense importance. The endothelium is typically the body's first contact with stimuli and its reaction to medical conditions of inflammation, disease, and body response are of great significance to understanding the physiology of numerous conditions ranging from heart, lung, and renal disease, to intestinal diseases. It is the purpose of this Master's thesis to analyze and optimize the ECIS system for making quantitative measurements of endothelial monolayer impedance, and accurately applying the results to a thoroughly reviewed analysis package in order to produce accurate cellular resistance parameters. The optimization of data acquisition (DAQ) is accomplished by systematic noise recognition, examination, and minimization; a task that has previously been unexplored in any studies using the ECIS system. Harmonic, 60 Hz, and Gaussian noise sources were well documented in unfiltered data and successfully minimized in the DAQ. Analog to digital (A/D) noise was found to be the lower limit of reducible noise and was properly documented and considered in analysis. Contamination of the electrode arrays from manufacturing processes and proper electrical connection were also found to be of concern to the proper functioning of the system. Analysis of the optimized acquired data was performed in the LabVIEW programming environment, as it offered a more flexible software package than that provided by the current commercially available ECIS system. The optimized system was applied to a further look into hand arm-vibration syndrome (HAVS) and it was concluded that the acceleration exposure dose, incorrectly calculated from the international standards, did not elicit an acute endothelial inflammation response by our measurements. The cumulative result of this study is that the ECIS system has been optimized and various unresolved sources of error were corrected for a more accurate real-time measurement of the endothelial monolayer barrier function in response to stimuli.
Book Synopsis Electroporation and Electrofusion in Cell Biology by : C.A. Jordan
Download or read book Electroporation and Electrofusion in Cell Biology written by C.A. Jordan and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
Book Synopsis Impedance Spectroscopy Based Portable Sensor Platforms by : Jon Engel Craven
Download or read book Impedance Spectroscopy Based Portable Sensor Platforms written by Jon Engel Craven and published by . This book was released on 2014 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: The work presented in this manuscript presents an investigation of impedance spectroscopy as a sensor platform detecting targets ranging from molecules to cells. By using impedance spectroscopy this was achieved in a real-time and label-free manner. In order to demonstrate this capability of impedance spectroscopy, two impedance spectroscopy methods will be employed: Electrical Impedance Spectroscopy (EIS) and Electric Cell-substrate Impedance Sensing (ECIS). EIS was used for the detection of fungicide agrochemicals from food samples. To demonstrate the ability of EIS to perform this function, samples of grape extract containing fungicides were tested to simulate real-world conditions. The sensors demonstrated limits of detection in the picomolar range, below the needed threshold level of detection. ECIS was used to detect and monitor cellular adherence and viability. To demonstrate the ability of ECIS to perform this function, cell cultures were monitored as cells adhered to and were removed from sensor electrodes.
Book Synopsis Modeling of Electronic Cell-substrate Impedance Sensing for Single Cell by : Dingkun Ren
Download or read book Modeling of Electronic Cell-substrate Impedance Sensing for Single Cell written by Dingkun Ren and published by . This book was released on 2013 with total page 50 pages. Available in PDF, EPUB and Kindle. Book excerpt: The thesis studies a three-dimensional rectangular hexahedron meshing (3D RHM) single-cell model to explore the cell behaviors for electronic cell-substrate impedance sensing (ECIS). In the model, a spatial network of resistors (R) and capacitors (C) is established to mimic a single-cell measurement system including electrodes, medium and mammalian cells. Unlike the existing ECIS models, which are limited by several approximations for current flow, spreading resistance and cell morphology, the proposed single-cell model is more flexible and enables us to provide high mesh resolution to reconstruct the real circumstances of cell and their surroundings. Moreover, the simulated results by single-cell model match the published measurement and simulation data by Thein and Huang. In addition, it is also demonstrated that the maximum change of total impedance (Z) between 100 Hz to 10 MHz varies based upon changes in cell properties, specifically the size of cell, cell membrane capacitance, cell-electrode distance, and cytoplasm resistivity, which is essential to understand the frequency-dependent cell behaviors.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2002 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Book Synopsis Tools, Techniques and Protocols for Monitoring Environmental Contaminants by : Satinder Kaur Brar
Download or read book Tools, Techniques and Protocols for Monitoring Environmental Contaminants written by Satinder Kaur Brar and published by Elsevier. This book was released on 2019-09-15 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tools, Techniques and Protocols for Monitoring Environmental Contaminants describes information on the strategic integration of available monitoring methods with molecular techniques, with a focus on omics (DNA, RNA and protein based) and molecular imprinted polymer and nanomaterial based advanced biosensors for environmental applications. It discusses the most commonly practiced analytic techniques, such as HPLC, MS, GCMS and traditional biosensors, giving an overview of the benefits of advanced biosensors over commonly practiced methods in the rapid and reliable assessment of environmental contaminants. As environmental contaminants have become one of the serious concerns in terms of their rapid growth and monitoring in the environment, which is often limited due to costly and laborious methods, this book provides a comprehensive update on their removal, the challenges they create for environmental regulatory agencies, and their diverse effects on terrestrial and aquatic environments. Provides methods for assessing and monitoring environmental contaminants Includes recent advancement in molecular techniques Outlines rapid environmental monitoring methods Explains the use of biosensors for environmental monitoring Reviews monitoring methods beyond conventional analytic techniques
Book Synopsis Electrochemical Biosensors by : Serge Cosnier
Download or read book Electrochemical Biosensors written by Serge Cosnier and published by CRC Press. This book was released on 2015-01-26 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analy
Book Synopsis Mems for Biomedical Applications by : Shekhar Bhansali
Download or read book Mems for Biomedical Applications written by Shekhar Bhansali and published by Elsevier. This book was released on 2012-07-18 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of Micro Electro Mechanical Systems (MEMS) in the biomedical field is leading to a new generation of medical devices. MEMS for biomedical applications reviews the wealth of recent research on fabrication technologies and applications of this exciting technology.The book is divided into four parts: Part one introduces the fundamentals of MEMS for biomedical applications, exploring the microfabrication of polymers and reviewing sensor and actuator mechanisms. Part two describes applications of MEMS for biomedical sensing and diagnostic applications. MEMS for in vivo sensing and electrical impedance spectroscopy are investigated, along with ultrasonic transducers, and lab-on-chip devices. MEMS for tissue engineering and clinical applications are the focus of part three, which considers cell culture and tissue scaffolding devices, BioMEMS for drug delivery and minimally invasive medical procedures. Finally, part four reviews emerging biomedical applications of MEMS, from implantable neuroprobes and ocular implants to cellular microinjection and hybrid MEMS.With its distinguished editors and international team of expert contributors, MEMS for biomedical applications provides an authoritative review for scientists and manufacturers involved in the design and development of medical devices as well as clinicians using this important technology. Reviews the wealth of recent research on fabrication technologies and applications of Micro Electro Mechanical Systems (MEMS) in the biomedical field Introduces the fundamentals of MEMS for biomedical applications, exploring the microfabrication of polymers and reviewing sensor and actuator mechanisms Considers MEMS for biomedical sensing and diagnostic applications, along with MEMS for in vivo sensing and electrical impedance spectroscopy
