Engineered Cell Manipulation for Biomedical Application

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Publisher :
ISBN 13 : 9784431551409
Total Pages : 280 pages
Book Rating : 4.5/5 (514 download)

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Book Synopsis Engineered Cell Manipulation for Biomedical Application by : Misturu Akashi

Download or read book Engineered Cell Manipulation for Biomedical Application written by Misturu Akashi and published by . This book was released on 2014-11-30 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Engineered Cell Manipulation for Biomedical Application

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Publisher : Springer
ISBN 13 : 4431551395
Total Pages : 271 pages
Book Rating : 4.4/5 (315 download)

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Book Synopsis Engineered Cell Manipulation for Biomedical Application by : Misturu Akashi

Download or read book Engineered Cell Manipulation for Biomedical Application written by Misturu Akashi and published by Springer. This book was released on 2014-10-16 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first to summarize new technologies for engineered cell manipulation. The contents focus on control of cellular functions by nanomaterials and control of three-dimensional cell–cell interactions. Control of cellular functions is important for cell differentiation, maturation, and activation, which generally are controlled by the addition of soluble cytokines or growth factors into cell culture dishes. Target antigen molecules can be efficiently delivered to the cytosol of the dendritic cells using the nanoparticle technique described here, and cellular functions such as dendritic cell maturation can be controlled easily and with precision. This book describes basic preparation of the nanoparticles, activation control of dendritic cells, immune function control, and in vivo application for various vaccination systems. The second type of control,that of cell–cell interaction, is important for tissue engineering in order to develop three-dimensional cellular constructs. To achieve in vitro engineering of three-dimensional human tissue constructs, cell–cell interaction must be controlled in three dimensions, but typical biological cell manipulation technique cannot accomplish this task. An engineered cell manipulation technique is necessary. In this book the authors describe the fabrication of nanofilms onto cell surfaces, development of three-dimensional cellular multilayers, and various applications of the cellular multilayers as three-dimensional human models. This important work will be highly informative for researchers and students in the fields of materials science, polymer science, biomaterials, medicinal science, nanotechnology, biotechnology, and biology.

Microfluidic Devices for Biomedical Applications

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Publisher : Woodhead Publishing
ISBN 13 : 9780857096975
Total Pages : 0 pages
Book Rating : 4.0/5 (969 download)

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Book Synopsis Microfluidic Devices for Biomedical Applications by : Xiujun James Li

Download or read book Microfluidic Devices for Biomedical Applications written by Xiujun James Li and published by Woodhead Publishing. This book was released on 2013-10-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries.

Culture of Cells for Tissue Engineering

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Publisher : John Wiley & Sons
ISBN 13 : 0471741809
Total Pages : 528 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Culture of Cells for Tissue Engineering by : Gordana Vunjak-Novakovic

Download or read book Culture of Cells for Tissue Engineering written by Gordana Vunjak-Novakovic and published by John Wiley & Sons. This book was released on 2006-03-31 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step, practicalguidance for the acquisition, manipulation, and use of cell sourcesfor tissue engineering. It offers a unique focus on tissueengineering methods for cell sourcing and utilization, combiningtheoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing,preparation, and characterization and the second part examinesspecific engineered tissues Each chapter covers: structural and functional properties oftissues, methodological principles, culture, cellselection/expansion, cell modifications, cell seeding, tissueculture, analytical assays, and a detailed description ofrepresentative studies End-of-chapter features include useful listings of sources forreagents, materials, and supplies, with the contact details of thesuppliers listed at the end of the book A section of elegant color plates to back up the figures in thechapters Culture of Cells for Tissue Engineering givesnovice and seasoned researchers in tissue engineering an invaluableresource. In addition, the text is suitable for professionals inrelated research, particularly in those areas where cell and tissueculture is a new or emerging tool.

Robotic Cell Manipulation

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Publisher : Academic Press
ISBN 13 : 0323852602
Total Pages : 548 pages
Book Rating : 4.3/5 (238 download)

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Book Synopsis Robotic Cell Manipulation by : Dong Sun

Download or read book Robotic Cell Manipulation written by Dong Sun and published by Academic Press. This book was released on 2022-05-31 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: Robotic Cell Manipulation introduces up-to-date research to realize this new theme of medical robotics. The book is organized in three levels: operation tools (e.g., optical tweezers, microneedles, dielectrophoresis, electromagnetic devices, and microfluidic chips), manipulation types (e.g., microinjection, transportation, rotation fusion, adhesion, separation, etc.), and potential medical applications (e.g., micro-surgery, biopsy, gene editing, cancer treatment, cell-cell interactions, etc.). The technology involves different fields such as robotics, automation, imaging, microfluidics, mechanics, materials, biology and medical sciences. The book provides systematic knowledge on the subject, covering a wide range of basic concepts, theories, methodology, experiments, case studies and potential medical applications. It will enable readers to promptly conduct a systematic review of research and become an essential reference for many new and experienced researchers entering this unique field. Introduces the applications of robot-assisted manipulation tools in various cell manipulation tasks Defines many essential concepts in association with the robotic cell manipulation field, including manipulation strategy and manipulation types Introduces basic concepts and knowledge on various manipulation devices and tasks Describes some cutting-edge cell manipulation technologies and case studies

Microtechnology for Cell Manipulation and Sorting

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Publisher : Springer
ISBN 13 : 3319441396
Total Pages : 281 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Microtechnology for Cell Manipulation and Sorting by : Wonhee Lee

Download or read book Microtechnology for Cell Manipulation and Sorting written by Wonhee Lee and published by Springer. This book was released on 2016-10-05 with total page 281 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.

Stem Cell Engineering

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Publisher : Springer Science & Business Media
ISBN 13 : 3642118658
Total Pages : 547 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Stem Cell Engineering by : Gerhard M. Artmann

Download or read book Stem Cell Engineering written by Gerhard M. Artmann and published by Springer Science & Business Media. This book was released on 2010-10-29 with total page 547 pages. Available in PDF, EPUB and Kindle. Book excerpt: The potential of stem cells for healing and disease prevention in all fields of medicine is tremendous and has revolutionized the high-tech biomedical research. In this book, many of the most prominent researchers discuss the challenging topics of stem cell engineering, for example: Ethical issues of stem cell research; technological challenges, stem cell growth and differentiation, therapeutic applications, bioreactors and bioprocesses, high throughput and microfluidic screening platforms, stem cell identification and sorting, intercellular signaling and engineered niches, novel approaches for embryonic and adult stem cell growth and differentiation, stem cells and drug discovery, screening platforms. Stem Cell Engineering offers valuable background and reference for both the public and professionals including industrial staffers, faculty, researchers, engineers, students and scientific journalists.

Micro and Nanotechnologies in Engineering Stem Cells and Tissues

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Publisher : John Wiley & Sons
ISBN 13 : 111857480X
Total Pages : 394 pages
Book Rating : 4.1/5 (185 download)

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Book Synopsis Micro and Nanotechnologies in Engineering Stem Cells and Tissues by : Murugan Ramalingam

Download or read book Micro and Nanotechnologies in Engineering Stem Cells and Tissues written by Murugan Ramalingam and published by John Wiley & Sons. This book was released on 2013-05-10 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: A cutting-edge look at the application of micro and nanotechnologies in regenerative medicine The area at the interface of micro/nanotechnology and stem cells/tissue engineering has seen an explosion of activity in recent years. This book provides a much-needed overview of these exciting developments, covering all aspects of micro and nanotechnologies, from the fundamental principles to the latest research to applications in regenerative medicine. Written and edited by the top researchers in the field, Micro and Nanotechnologies in Engineering Stem Cells and Tissues describes advances in material systems along with current techniques available for cell, tissue, and organ studies. Readers will gain tremendous insight into the state of the art of stem cells and tissue engineering, and learn how to use the technology in their own research or clinical trials. Coverage includes: Technologies for controlling or regulating stem cell and tissue growth Various engineering approaches for stem cell, vascular tissue, and bone regeneration The design and processing of biocompatible polymers and other biomaterials Characterization of the interactions between cells and biomaterials Unrivaled among books of this kind, Micro and Nanotechnologies in Engineering Stem Cells and Tissues is the ultimate forward-looking reference for researchers in numerous disciplines, from engineering and materials science to biomedicine, and for anyone wishing to understand the trends in this transformative field.

Autonomous Robot-Aided Optical Manipulation for Biological Cells

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Publisher : Academic Press
ISBN 13 : 0128235926
Total Pages : 190 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Autonomous Robot-Aided Optical Manipulation for Biological Cells by : Mingyang Xie

Download or read book Autonomous Robot-Aided Optical Manipulation for Biological Cells written by Mingyang Xie and published by Academic Press. This book was released on 2021-05-12 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Autonomous Robot-Aided Optical Manipulation for Biological Cells gives a systematically and almost self-contained description of the many facets of modeling, sensing, and control techniques or experimentally exploring emerging trends in optical manipulation of biological cell in micro/nanorobotics systems. To achieve biomedical applications, reliability design, modeling, and precision control are vitally important for the development of engineering systems. With the advances in modeling, sensing, and control techniques, it is opportunistic to exploit them for the benefit of reliability design, actuation, and precision control of micro/nanomanipulation systems to expanding the applications of robot at the micro and nano scales, especially in biomedical engineering. This book presents new techniques in reliability modeling and advanced control of robot-aided optical manipulation of biological cells systems. The book will be beneficial to the researchers within robotics, mechatronics, biomedical engineering, and automatic control society, including both academic and industrial parts. Provides a series of latest results in, including but not limited to, design, sensing, actuation, modeling, and control of micro/nano manipulation system using optical tweezers Gives recent advances of theory, technological aspects, and applications of advanced sensing, actuation, modeling and control methodologies in biomedical engineering applications Offers simulation and experimental results in each chapter in order to reflect the biomedical engineering practice, yet demonstrate the main focus of the developed design, analysis and synthesis approaches

Molecular, Cellular, and Tissue Engineering

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Publisher : CRC Press
ISBN 13 : 1439825319
Total Pages : 1891 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Molecular, Cellular, and Tissue Engineering by : Joseph D. Bronzino

Download or read book Molecular, Cellular, and Tissue Engineering written by Joseph D. Bronzino and published by CRC Press. This book was released on 2018-10-08 with total page 1891 pages. Available in PDF, EPUB and Kindle. Book excerpt: Known as the bible of biomedical engineering, The Biomedical Engineering Handbook, Fourth Edition, sets the standard against which all other references of this nature are measured. As such, it has served as a major resource for both skilled professionals and novices to biomedical engineering. Molecular, Cellular, and Tissue Engineering, the fourth volume of the handbook, presents material from respected scientists with diverse backgrounds in molecular biology, transport phenomena, physiological modeling, tissue engineering, stem cells, drug delivery systems, artificial organs, and personalized medicine. More than three dozen specific topics are examined, including DNA vaccines, biomimetic systems, cardiovascular dynamics, biomaterial scaffolds, cell mechanobiology, synthetic biomaterials, pluripotent stem cells, hematopoietic stem cells, mesenchymal stem cells, nanobiomaterials for tissue engineering, biomedical imaging of engineered tissues, gene therapy, noninvasive targeted protein and peptide drug delivery, cardiac valve prostheses, blood substitutes, artificial skin, molecular diagnostics in personalized medicine, and bioethics.

Cell-Inspired Materials and Engineering

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Publisher : Springer
ISBN 13 : 9783030559267
Total Pages : 257 pages
Book Rating : 4.5/5 (592 download)

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Book Synopsis Cell-Inspired Materials and Engineering by : Dan Ohtan Wang

Download or read book Cell-Inspired Materials and Engineering written by Dan Ohtan Wang and published by Springer. This book was released on 2022-04-16 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights cutting-edge studies in the development of cell-inspired biomaterials and synthetic materials that manipulate cell functions and provide the next generation with contemporary tools for treating complex human diseases. It explores the convergence of synthetic materials with cell and molecular biology and surveys how functional materials, when patterned with spatial and temporal precision, can be used effectively to maintain cell proliferation and phenotype in vitro, to trigger specific cell functions, and to redirect cell-fate decisions. Human stem cells are a frequently discussed subject in this book. This is an ideal book for students, cell biologists, researchers interested in interdisciplinary research, and biomedical engineers. This book also: Highlights successfully developed technologies in cell engineering that make possible new therapeutic development for previously untreatable conditions Covers topics including bio-inspired micro patterning, DNA origami technology, synthetic NOS inspired by compartmentalized signaling in cells, and light-induced depolarization of the cell membrane Illustrates in detail the use of stem cells and synthetic scaffolds to model ethically sensitive embryonic tissues and organs

Biomaterials for Stem Cell Therapy

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Publisher : CRC Press
ISBN 13 : 1466576391
Total Pages : 664 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis Biomaterials for Stem Cell Therapy by : Loredana De Bartolo

Download or read book Biomaterials for Stem Cell Therapy written by Loredana De Bartolo and published by CRC Press. This book was released on 2013-01-28 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focused on stem cell applications, this book bridges the fields of biomaterials, offering new insights into constructing and regenerating tissues and organs. Its unique feature is linking diseases of the human body to current thinking on how to deal with them in the context of current concepts and technologies by means of an in-depth focus on biomaterials. The book assembles recent advances and covers a range of topics related to stem cell biology, biomaterials and technological approaches such as bioreactors written by top researchers in the field. Stem cells of both embryonic and adult origin are discussed with applications ranging, but not limited to, nerve regeneration, liver, pancreas, skin, trachea, cartilage and bone repair and cardiovascular therapy. Developments in the field reflecting the design and construction of the human body and its principal anatomy are discussed from a materials point of view. The book will be a valuable tool for biomaterial scientists, tissue engineers, clinicians as well as stem cell biologists involved in basic research and applications of adult and embryonic stem cells. It will also be a source of reference for students in biotechnology, biomedical engineering, biology, biochemistry, materials sciences, pharmaceuticals, and veterinary and human medicine.

Standardisation in Cell and Tissue Engineering

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Publisher : Elsevier
ISBN 13 : 0857098721
Total Pages : 276 pages
Book Rating : 4.8/5 (57 download)

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Book Synopsis Standardisation in Cell and Tissue Engineering by : V Salih

Download or read book Standardisation in Cell and Tissue Engineering written by V Salih and published by Elsevier. This book was released on 2013-07-31 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of ‘best practice’ methods in the area of tissue engineering to help ensure the creation of safe, high quality products. Standardisation in cell and tissue engineering introduces concepts and current practice in the field of cell and tissue engineering to a wide audience and aims to provide awareness of the importance of standardisation in this area while suggesting directions for further investigation. Part one provides an overview of methods for cell and tissue engineering and includes chapters on the fundamentals of cell and matrix biology for tissue engineering, 3D collagen biomatrix development, and control and vascularisation of tissue-engineered constructs. Part two begins with a chapter exploring the methods and protocols of standardisation in cell and tissue engineering before moving on to highlight issues of quality control in cell and tissue engineering, standardised chemical analysis and testing of biomaterials and principles of good laboratory practice (GLP) for in vitro cell culture applications. Standardisation in cell and tissue engineering is a standard reference for leading research groups, government agencies, regulatory bodies, and researchers and technicians at all levels across the whole range of disciplines using cell culture within the pharmaceutical, biotechnology and biomedical industries. Introduces concepts and current practice in the field of cell and tissue engineering Highlights the importance of standardisation in cell and tissue engineering and suggests directions for further investigation Explores methods and protocols of standardisation in cell and tissue engineering and issues of quality control in cell and tissue engineering

Building Tissues

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Publisher : CRC Press
ISBN 13 : 0429768737
Total Pages : 342 pages
Book Rating : 4.4/5 (297 download)

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Book Synopsis Building Tissues by : Joseph W. Freeman

Download or read book Building Tissues written by Joseph W. Freeman and published by CRC Press. This book was released on 2018-11-08 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering uniquely applies concepts and techniques from biology and engineering in order to heal or produce new tissues after disease or traumatic injury. A successful tissue engineer must have knowledge of cellular biology, cell signaling, extracellular matrix development, and tissue structure and integrate it with the application of stresses and strains, mass transfer, mechanical properties, and heat transfer. In order to train the next generation of successful tissue engineers, this text gives the reader a background in both the engineering and biology associated with tissue engineering. In reading this text, students will learn about these two different areas of study and how they can be integrated with one another to understand tissues in the human body and solve biomedical problems. Students will be introduced to definitions of engineering concepts, the practical use of stress-strain relationships, material strength, mass transfer, and heat transfer. Through examples and problems, students will apply engineering equations to medical and biomedical situations including actual tissue engineering problems. Students will be introduced to a variety of cell and tissue types and be given the background information necessary to apply the use of cells to the growth and development of new tissues. Students will learn how to select the proper material for the replacement of a particular tissue and why it is important to know about the mechanical properties and degradability of a material prior to implantation. Students will learn how the application of force, material selection, and changes in temperature can positively or negatively affect cell behavior and tissue development. Tissue structure will be described and students will learn about the direct relationship between the structure of a tissue and its properties.

Polymers for Biomedical Applications

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Author :
Publisher : American Chemical Society
ISBN 13 :
Total Pages : 442 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Polymers for Biomedical Applications by : Anil Mahapatro

Download or read book Polymers for Biomedical Applications written by Anil Mahapatro and published by American Chemical Society. This book was released on 2008-04-17 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research on applications of polymers for biomedical applications has increased dramatically to find improved medical plastics for this rapidly evolving field. This book brings together various aspects of recent research and developments within academia and industry related to polymers for biomedical applications.

Microfluidic Devices for Biomedical Applications

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Publisher : Elsevier
ISBN 13 : 0857097040
Total Pages : 676 pages
Book Rating : 4.8/5 (57 download)

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Book Synopsis Microfluidic Devices for Biomedical Applications by : Xiujun James Li

Download or read book Microfluidic Devices for Biomedical Applications written by Xiujun James Li and published by Elsevier. This book was released on 2013-10-31 with total page 676 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores in detail a wide range of medical applications Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies Considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering

Biomedical Applications of Microfluidic Devices

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Publisher : Academic Press
ISBN 13 : 0128187921
Total Pages : 352 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Biomedical Applications of Microfluidic Devices by : Michael R. Hamblin

Download or read book Biomedical Applications of Microfluidic Devices written by Michael R. Hamblin and published by Academic Press. This book was released on 2020-11-12 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical Applications of Microfluidic Devices introduces the subject of microfluidics and covers the basic principles of design and synthesis of actual microchannels. The book then explores how the devices are coupled to signal read-outs and calibrated, including applications of microfluidics in areas such as tissue engineering, organ-on-a-chip devices, pathogen identification, and drug/gene delivery. This book covers high-impact fields (microarrays, organ-on-a-chip, pathogen detection, cancer research, drug delivery systems, gene delivery, and tissue engineering) and shows how microfluidics is playing a key role in these areas, which are big drivers in biomedical engineering research. This book addresses the fundamental concepts and fabrication methods of microfluidic systems for those who want to start working in the area or who want to learn about the latest advances being made. The subjects covered are also an asset to companies working in this field that need to understand the current state-of-the-art. The book is ideal for courses on microfluidics, biosensors, drug targeting, and BioMEMs, and as a reference for PhD students. The book covers the emerging and most promising areas of biomedical applications of microfluidic devices in a single place and offers a vision of the future. Covers basic principles and design of microfluidics devices Explores biomedical applications to areas such as tissue engineering, organ-on-a-chip, pathogen identification, and drug and gene delivery Includes chemical applications in organic and inorganic chemistry Serves as an ideal text for courses on microfluidics, biosensors, drug targeting, and BioMEMs, as well as a reference for PhD students