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Dna Translocation And Cell Electroporation In Micro And Nanofluidic Devices
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Book Synopsis DNA Translocation and Cell Electroporation in Micro and Nanofluidic Devices by : Cherry Gupta
Download or read book DNA Translocation and Cell Electroporation in Micro and Nanofluidic Devices written by Cherry Gupta and published by . This book was released on 2015 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: Besides maintaining high cell viability and achieving dose control, an important feature of NEP is the delivery of large molecules such as plasmids and quantum dots directly into the cell cytosol. In contrast, delivery of large plasmids during bulk electroporation, wherein cells and genes/plasmids are mixed in a buffered medium and an external electric field is applied across the mixture which electroporates the cells, is via formation of cell membrane bound aggregates which get endocytosed post pulsation. Various mechanisms of DNA transport across the membrane have been proposed including passive diffusion of DNA through pores formed during electroporation, electrophoretic transfer of DNA into the cytosol through membrane pores, and electrically induced trapping of DNA in the endosome-like vesicles. In this study we visualized the transport of DNA molecules during NEP to understand the associated delivery mechanism.
Book Synopsis Micro and Nanofluidic Devices by : Venkata Raghavendra Subrahmanya Sarma Mokkapati
Download or read book Micro and Nanofluidic Devices written by Venkata Raghavendra Subrahmanya Sarma Mokkapati and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Single DNA Dynamics and Cell Electroporation in Micro/nanofluidics by : Wei-Ching Liao
Download or read book Single DNA Dynamics and Cell Electroporation in Micro/nanofluidics written by Wei-Ching Liao and published by . This book was released on 2012 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: A new nanochannel electroporation (NEP) was developed recently in our laboratory to realize dosage-controlled delivery. The nanochannel was fabricated by using large DNA molecules as building blocks in a new DNA combing and imprinting process. Large DNA was stretched to generate the nanowire array that was then used to fabricate the nanochannel array. During the electroporation, transfection agents including DNA molecules were electrophoretically driven through microchannel, nanochannel, and finally into individual cells. Therefore, DNA dynamics and cell electroporation are two main components in the process of NEP. In this dissertation, we study the DNA stretching dynamics in DNA combing, DNA electrophoretic dynamics in the microchannel and nanochannel, cell electroporation mechanism in NEP, and the detection of membrane poration.
Book Synopsis Micro- and Nanofluidic Devices for Complexed DNA Analysis at the Single Molecule Level by : 史利南
Download or read book Micro- and Nanofluidic Devices for Complexed DNA Analysis at the Single Molecule Level written by 史利南 and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fabrication of Micro- und Nanofluidic Devices for Single DNA Molcule Analysis by :
Download or read book Fabrication of Micro- und Nanofluidic Devices for Single DNA Molcule Analysis written by and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fabrication and Characterization of Membrane Electroporation Devices for Drug/ Gene Delivery by : Kun Yeh Chiang
Download or read book Fabrication and Characterization of Membrane Electroporation Devices for Drug/ Gene Delivery written by Kun Yeh Chiang and published by . This book was released on 2008 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Electroporation is a common technology for delivering genes and small or large molecules into cells. Local electroporation using a thin membrane with random micro- pores has shown significant enhancements over bulk electroporation on transfection efficiency and cell viability. In this study, we demonstrate a much less invasive and more efficient gene delivery method - membrane sandwich electroporation (MSE). We use a poly (e-caprolacton) (PCL) membrane with well defined micro-pore arrays for cell-trapping and gene delivery. Cells are immobilized on the micro-pore arrays by vacuum for localized electroporation. The localized electroporation is realized by applying voltage pulse or DC signal to achieve dielectric breakdown of the cell membrane. The conically circular pore arrays provide a great potential gradient when an electrical field is applied. This provides an efficient way to accelerate the rigid carriers to a high momentum for delivering species inside cells without damage to cell membrane. For device fabrication, the PCL membrane films are replicated from patterned SU-8 structures. The hollow microarrays in SU-8 with 10l̃5 um in height are transferred to a PDMS (Polydimethylsiloxane) film by soft lithography. The PDMS film with transferred circular pillar patterns is used as the mold for another replication of uniform PCL thin polymer films with a thickness of 5 to 8 um. NIH 3T3 cells are trapped and immobilized on micro-pore arrays by vacuum. Opti-MEM ITM reduced-serum medium is filled into the channels and the center reservoir before the DNA (pEGFP) sample is loaded into the inlet reservoir. Electroporation is carried out followed by a cell culture process for transfection efficiency characterization. The fluorescence images of NIH 3T3 cells after DNA delivery by electroporation in a MSE device with 5 [mu]m pores array indicate a uniform gene delivery. Evidently, the cells are uniformly trapped on the micro-pores array and each cell experiences the same condition during electroporation. Impedance measurements and device modeling are performed to study the behaviors at different biases and frequencies. DNA transfection with fluorescence expression by using probe station electroporation is able to be observed from 1 Volt to 40 Volt. Such devices for local electroporation with well defined micro-pore array structures have demonstrated great improvement in comparison with devices with random pores.
Book Synopsis Micro/Nanofluidic Devices for Single Cell Analysis by : Fan-Gang Tseng
Download or read book Micro/Nanofluidic Devices for Single Cell Analysis written by Fan-Gang Tseng and published by MDPI. This book was released on 2018-10-04 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Micro/Nanofluidic Devices for Single Cell Analysis" that was published in Micromachines
Book Synopsis Engineering of Micro/Nano Biosystems by : Gregory Barbillon
Download or read book Engineering of Micro/Nano Biosystems written by Gregory Barbillon and published by Springer. This book was released on 2019-08-02 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: This tutorial book offers an in-depth overview of the fundamental principles of micro/nano technologies and devices related to sensing, actuation and diagnosis in fluidics and biosystems. Research in the MEMS/NEMS and lab-on-chip fields has seen rapid growth in both academic and industrial domains, as these biodevices and systems are increasingly replacing traditional large size diagnostic tools. This book is unique in describing not only the devices and technologies but also the basic principles of their operation. The comprehensive description of the fabrication, packaging and principles of micro/nano biosystems presented in this book offers guidance for researchers designing and implementing these biosystems across diverse fields including medical, pharmaceutical and biological sciences. The book provides a detailed overview of the fundamental mechanical, optical, electrical and magnetic principles involved, together with the technologies required for the design, fabrication and characterization of micro/nano fluidic systems and bio-devices. Written by a collaborative team from France and Korea, the book is suitable for academics, researchers, advanced level students and industrial manufacturers.
Book Synopsis Cell-based Biosensors by : Qingjun Liu
Download or read book Cell-based Biosensors written by Qingjun Liu and published by Artech House. This book was released on 2009-10 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by recognized experts the field, this leading-edge resource is the first book to systematically introduce the concept, technology, and development of cell-based biosensors. You find details on the latest cell-based biosensor models and novel micro-structure biosensor techniques. Taking an interdisciplinary approach, this unique volume presents the latest innovative applications of cell-based biosensors in a variety of biomedical fields. The book also explores future trends of cell-based biosensors, including integrated chips, nanotechnology and microfluidics. Over 140 illustrations help clarify key topics throughout the book.
Download or read book Food Bioactives written by Munish Puri and published by Springer. This book was released on 2017-04-07 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on various types of bioactive compounds, including secondary metabolites, oligosaccharides, polysaccharides, flavonoids, peptides/proteins, carotenoid pigments, quinones, terpenes, and polyunsaturated fatty acids, and presents an overview of their nutraceutical activities. It covers the current status and future potential of food compounds, as well as extraction technologies for bioactives derived from plant, fungi and marine-derived bioactive agents. Finally, health-promoting effects of plant, fungi and marine-derived bioactive agents are discussed. Chapters come from top researchers in this area from around the globe. The volume caters to the needs of undergraduate and post-graduate students in the area of food biotechnology, food bioprocessing, biotechnology, food engineering, etc., and also contains information pertinent to researchers.
Book Synopsis Industrial Pharmaceutical Biotechnology by : Heinrich Klefenz
Download or read book Industrial Pharmaceutical Biotechnology written by Heinrich Klefenz and published by Wiley-VCH. This book was released on 2002-04-22 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on pharmaceutical biotechnology as a key area of life sciences. The complete range of concepts, processes and technologies of biotechnology is applied in modern industrial pharmaceutical research, development and production. The results of genome sequencing and studies of biological-genetic function are combined with chemical, micro-electronic and microsystem technology to produce medical devices and diagnostic biochips. A multitude of biologically active molecules is expanded by additional novel structures created with newly arranged gene clusters and bio-catalytic chemical processes. New organisational structures in the co-operation of institutes, companies and networks enable faster knowledge and product development and immediate application of the results of research and process development. This book is the ideal source of information for scientists and engineers in research and development, for decision-makers in biotech, pharma and chemical corporations, as well as for research institutes, but also for founders of biotech companies and people working for venture capital corporations.
Book Synopsis BioMEMS and Biomedical Nanotechnology by : Mihrimah Ozkan
Download or read book BioMEMS and Biomedical Nanotechnology written by Mihrimah Ozkan and published by Springer Science & Business Media. This book was released on 2007-04-03 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices with applications to Genomics and Proteomics. Topics include gene expression profiling utilizing microarray technology; imaging and sensing for gene detection and use in DNA analysis; and coverage of advanced microfluidic devices and the Humane Genome Project.
Book Synopsis Micro and Nano Technologies in Bioanalysis by : James W. Lee
Download or read book Micro and Nano Technologies in Bioanalysis written by James W. Lee and published by Methods in Molecular Biology. This book was released on 2009-07-21 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, large-scale advances in technology have led to greater understanding of the world at the biomolecular level. In this book, expert researchers from across the globe explore the technology which makes this analysis possible.
Book Synopsis Molecular- and Nano-Tubes by : Oliver Hayden
Download or read book Molecular- and Nano-Tubes written by Oliver Hayden and published by Springer Science & Business Media. This book was released on 2011-08-04 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular- and Nano-Tubes summarizes recent advancements in the synthesis, fabrication and applications of tubular structures. An interdisciplinary overview of innovative science focused on tubular structures is provided. The reader is offered an overview of the different fields that molecular and nano tubes appear in, in order to learn the fundamental basics as well as the applications of these materials. This book also: Shows how nanotechnology creates novel materials by crossing the barriers between biology and material science, electronics and optics, medicine and more Demonstrates that tubes are a fundamental element in nature and used in disparate applications such as ion channels and carbon nanotubes Molecular- and Nano-Tubes is an ideal volume for researchers and engineers working in materials science and nanotechnology.
Book Synopsis The Minimal Cell by : Pier Luigi Luisi
Download or read book The Minimal Cell written by Pier Luigi Luisi and published by Springer Science & Business Media. This book was released on 2010-11-01 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last ten years there has been a considerable increase of interest on the notion of the minimal cell. With this term we usually mean a cell-like structure containing the minimal and sufficient number of components to be defined as alive, or at least capable of displaying some of the fundamental functions of a living cell. In fact, when we look at extant living cells we realize that thousands of molecules are organized spatially and functionally in order to realize what we call cellular life. This fact elicits the question whether such huge complexity is a necessary condition for life, or a simpler molecular system can also be defined as alive. Obviously, the concept of minimal cell encompasses entire families of cells, from totally synthetic cells, to semi-synthetic ones, to primitive cell models, to simple biomimetic cellular systems. Typically, in the experimental approach to the construction of minimal the main ingredient is the compartment. Lipid vesicles (liposomes) are used to host simple and complex molecular transformations, from single or multiple enzymic reactions, to polymerase chain reactions, to gene expression. Today this research is seen as part of the broader scenario of synthetic biology but it is rooted in origins of life studies, because the construction of a minimal cell might provide biophysical insights into the origins of primitive cells, and the emergence of life on earth. The volume provides an overview of physical, biochemical and functional studies on minimal cells, with emphasis to experimental approaches. 15 International experts report on their innovative contributions to the construction of minimal cells.
Book Synopsis The Handbook of Nanomedicine by : Kewal K. Jain
Download or read book The Handbook of Nanomedicine written by Kewal K. Jain and published by Humana Press. This book was released on 2017-03-20 with total page 661 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomedicine is defined as the application of nanobiotechnology in clinical medicine, which is currently being used to research the pathomechanism of disease, refine molecular diagnostics, and aid in the discovery, development, and delivery of drugs. In The Handbook of Nanomedicine, Third Edition, Prof. Kewal K. Jain updates, reorganizes, and replaces information in the comprehensive second edition in order to capture the most recent advances in this dynamic field. Important components of nanomedicine such as drug delivery via nanobiotechnology and nanopharmaceuticals as well as nanooncology, where the greatest number of advances are occurring, are covered extensively. As this text is aimed at nonmedical scientists, pharmaceutical personnel, as well as physicians, descriptions of the technology involved and other medical terminology are kept as clear and simple as possible. In depth and cutting-edge, The Handbook of Nanomedicine, Third Edition informs its readers of the ever-growing field of nanomedicine, destined to play a significant role in the future of healthcare.
Book Synopsis The Delivery of Nanoparticles by : Abbass A. Hashim
Download or read book The Delivery of Nanoparticles written by Abbass A. Hashim and published by BoD – Books on Demand. This book was released on 2012-05-16 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoparticle is a general challenge for today's technology and the near future observations of science. Nanoparticles cover mostly all types of sciences and manufacturing technologies. The properties of this particle are flying over today scientific barriers and have passed the limitations of conventional sciences. This is the reason why nanoparticles have been evaluated for the use in many fields. InTech publisher and the contributing authors of this book in nanoparticles are all overconfident to invite all scientists to read this new book. The book's potential was held until it was approached by the art of exploring the most advanced research in the field of nano-scale particles, preparation techniques and the way of reaching their destination. 25 reputable chapters were framed in this book and there were alienated into four altered sections; Toxic Nanoparticles, Drug Nanoparticles, Biological Activities and Nano-Technology.
