DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry

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Publisher :
ISBN 13 :
Total Pages : 89 pages
Book Rating : 4.:/5 (684 download)

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Book Synopsis DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry by :

Download or read book DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry written by and published by . This book was released on 1998 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first demonstration of the laser in the 1960's, lasers have found numerous applications in analytical chemistry. In this work, two different applications are described, namely, DNA sequencing with capillary gel electrophoresis and single cell analysis with mass spectrometry. Two projects are described in which high-speed DNA separations with capillary gel electrophoresis were demonstrated. In the third project, flow cytometry and mass spectrometry were coupled via a laser vaporization/ionization interface and individual mammalian cells were analyzed. First, DNA Sanger fragments were separated by capillary gel electrophoresis. A separation speed of 20 basepairs per minute was demonstrated with a mixed poly(ethylene oxide) (PEO) sieving solution. In addition, a new capillary wall treatment protocol was developed in which bare (or uncoated) capillaries can be used in DNA sequencing. Second, a temperature programming scheme was used to separate DNA Sanger fragments. Third, flow cytometry and mass spectrometry were coupled with a laser vaporization/ionization interface.

DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry

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Publisher :
ISBN 13 :
Total Pages : 308 pages
Book Rating : 4.:/5 (396 download)

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Book Synopsis DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry by : Nga-kit Fung

Download or read book DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry written by Nga-kit Fung and published by . This book was released on 1998 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mass detection limit of 20 amol (S/N = 3) of serotonin in a single laser pulse was demonstrated. Determination of serotonin and histamine in individual rat peritoneal mast cells (RPMC) was demonstrated. The intracellular contents of serotonin and histamine were found to vary considerably. No correlation was found between the amount of serotonin and histamine.

Analytical Techniques In DNA Sequencing

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Publisher : CRC Press
ISBN 13 : 1420029088
Total Pages : 252 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Analytical Techniques In DNA Sequencing by : Brian K. Nunnally

Download or read book Analytical Techniques In DNA Sequencing written by Brian K. Nunnally and published by CRC Press. This book was released on 2005-06-23 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: While one marvels at the DNA generated by the Human Genome Project, perhaps just as significant and fascinating is the development of the amazing analytical technology that has permitted us entry into the genomic era and a whole new level of scientific understanding. Analytical Techniques in DNA Sequencing takes a look at the various DNA

Handbook of Capillary Electrophoresis, Second Edition

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Publisher : CRC Press
ISBN 13 : 9780849324987
Total Pages : 918 pages
Book Rating : 4.3/5 (249 download)

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Book Synopsis Handbook of Capillary Electrophoresis, Second Edition by : James P. Landers

Download or read book Handbook of Capillary Electrophoresis, Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

New High Throughput Technologies for DNA Sequencing and Genomics

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Publisher : Elsevier
ISBN 13 : 0080471285
Total Pages : 399 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis New High Throughput Technologies for DNA Sequencing and Genomics by : Keith R. Mitchelson

Download or read book New High Throughput Technologies for DNA Sequencing and Genomics written by Keith R. Mitchelson and published by Elsevier. This book was released on 2011-09-22 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into today's multibillion dollar industry. This growth has spurred the development of new sequencing technologies that do not involve either electrophoresis or Sanger sequencing chemistries. Sequencing by Synthesis (SBS) involves multiple parallel micro-sequencing addition events occurring on a surface, where data from each round is detected by imaging. New High Throughput Technologies for DNA Sequencing and Genomics is the second volume in the Perspectives in Bioanalysis series, which looks at the electroanalytical chemistry of nucleic acids and proteins, development of electrochemical sensors and their application in biomedicine and in the new fields of genomics and proteomics. The authors have expertly formatted the information for a wide variety of readers, including new developments that will inspire students and young scientists to create new tools for science and medicine in the 21st century. Reviews of complementary developments in Sanger and SBS sequencing chemistries, capillary electrophoresis and microdevice integration, MS sequencing and applications set the framework for the book. * 'Hot Topic' with DNA sequencing continuing as a major research activity in many areas of life science and medicine. * Bringing together new developments in DNA sequencing technology * Reviewing issues relevant to the new applications used

Single Cell ‘Omics of Neuronal Cells

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Publisher : Springer Nature
ISBN 13 : 107162525X
Total Pages : 302 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Single Cell ‘Omics of Neuronal Cells by : Jonathan V. Sweedler

Download or read book Single Cell ‘Omics of Neuronal Cells written by Jonathan V. Sweedler and published by Springer Nature. This book was released on 2022-08-10 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell ‘Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

Single Molecule and Single Cell Sequencing

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Publisher : Springer
ISBN 13 : 9811360375
Total Pages : 150 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Single Molecule and Single Cell Sequencing by : Yutaka Suzuki

Download or read book Single Molecule and Single Cell Sequencing written by Yutaka Suzuki and published by Springer. This book was released on 2019-04-09 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians.

Single Cell Analysis by Capillary Electrophoresis

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (654 download)

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Book Synopsis Single Cell Analysis by Capillary Electrophoresis by :

Download or read book Single Cell Analysis by Capillary Electrophoresis written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Analysis and Genome Discovery

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Publisher : Wiley-Blackwell
ISBN 13 :
Total Pages : 400 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Molecular Analysis and Genome Discovery by : Ralph Rapley

Download or read book Molecular Analysis and Genome Discovery written by Ralph Rapley and published by Wiley-Blackwell. This book was released on 2004-06-28 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This advanced level textbook provides a comprehensive overview of recent developments in the area of molecular based diagnostics of disease markers. Examples are given where this new area of bioscience has or may be successfully applied.

Single Cell Analysis in Biotechnology and Systems Biology

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Publisher : MDPI
ISBN 13 : 3038421936
Total Pages : 237 pages
Book Rating : 4.0/5 (384 download)

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Book Synopsis Single Cell Analysis in Biotechnology and Systems Biology by : Fan-Gang Tseng

Download or read book Single Cell Analysis in Biotechnology and Systems Biology written by Fan-Gang Tseng and published by MDPI. This book was released on 2018-10-01 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS

Single Cell Metabolism

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Publisher : Humana
ISBN 13 : 9781493998296
Total Pages : 0 pages
Book Rating : 4.9/5 (982 download)

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Book Synopsis Single Cell Metabolism by : Bindesh Shrestha

Download or read book Single Cell Metabolism written by Bindesh Shrestha and published by Humana. This book was released on 2019-09-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field.

Rapid Sizing of DNA and Analysis of Single Cells Using Capillary Electrophoresis

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Publisher :
ISBN 13 :
Total Pages : 430 pages
Book Rating : 4.:/5 (479 download)

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Book Synopsis Rapid Sizing of DNA and Analysis of Single Cells Using Capillary Electrophoresis by : Woei G. Tan

Download or read book Rapid Sizing of DNA and Analysis of Single Cells Using Capillary Electrophoresis written by Woei G. Tan and published by . This book was released on 2000 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Capillary Gel Electrophoresis

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Publisher : Newnes
ISBN 13 : 0080931359
Total Pages : 391 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Precision Medicine for Investigators, Practitioners and Providers

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Publisher : Academic Press
ISBN 13 : 0128191791
Total Pages : 640 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Precision Medicine for Investigators, Practitioners and Providers by : Joel Faintuch

Download or read book Precision Medicine for Investigators, Practitioners and Providers written by Joel Faintuch and published by Academic Press. This book was released on 2019-11-16 with total page 640 pages. Available in PDF, EPUB and Kindle. Book excerpt: Precision Medicine for Investigators, Practitioners and Providers addresses the needs of investigators by covering the topic as an umbrella concept, from new drug trials to wearable diagnostic devices, and from pediatrics to psychiatry in a manner that is up-to-date and authoritative. Sections include broad coverage of concerning disease groups and ancillary information about techniques, resources and consequences. Moreover, each chapter follows a structured blueprint, so that multiple, essential items are not overlooked. Instead of simply concentrating on a limited number of extensive and pedantic coverages, scholarly diagrams are also included. Provides a three-pronged approach to precision medicine that is focused on investigators, practitioners and healthcare providers Covers disease groups and ancillary information about techniques, resources and consequences Follows a structured blueprint, ensuring essential chapters items are not overlooked

New Technologies for Genomics and Proteomics

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Publisher :
ISBN 13 :
Total Pages : 420 pages
Book Rating : 4.:/5 (57 download)

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Book Synopsis New Technologies for Genomics and Proteomics by : Haihong Zhou

Download or read book New Technologies for Genomics and Proteomics written by Haihong Zhou and published by . This book was released on 2000 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein Sequencing and Identification Using Tandem Mass Spectrometry

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Publisher : Wiley-Interscience
ISBN 13 : 0471231886
Total Pages : 320 pages
Book Rating : 4.4/5 (712 download)

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Book Synopsis Protein Sequencing and Identification Using Tandem Mass Spectrometry by : Michael Kinter

Download or read book Protein Sequencing and Identification Using Tandem Mass Spectrometry written by Michael Kinter and published by Wiley-Interscience. This book was released on 2005-04-12 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: Peptide fragmentation and interpretation of product ion spectra Basic polyacrylamide gel electrophoresis Preparation of protein digests for sequencing experiments Mass spectrometric analysis using capillary liquid chromatography Techniques for protein identification by database searches Characterization of modified peptides using tandem mass spectrometry And much more

Novel Approaches to DNA Sequencing

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Publisher :
ISBN 13 :
Total Pages : 88 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Novel Approaches to DNA Sequencing by : Sarah Marie Lee

Download or read book Novel Approaches to DNA Sequencing written by Sarah Marie Lee and published by . This book was released on 1996 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: