Development Of A Microfluidic Flow Cytometry Platform With Fluorescence And Light Scattering Detection For The Rapid Characterization Of Circulating Tumor Cells

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Microscale Acoustofluidics

Author : Thomas Laurell
Publisher : Royal Society of Chemistry
ISBN 13 : 1849737061
Total Pages : 593 pages
Book Rating : 4.8/5 (497 download)

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Book Synopsis Microscale Acoustofluidics by : Thomas Laurell

Download or read book Microscale Acoustofluidics written by Thomas Laurell and published by Royal Society of Chemistry. This book was released on 2014-12-08 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: The manipulation of cells and microparticles within microfluidic systems using external forces is valuable for many microscale analytical and bioanalytical applications. Acoustofluidics is the ultrasound-based external forcing of microparticles with microfluidic systems. It has gained much interest because it allows for the simple label-free separation of microparticles based on their mechanical properties without affecting the microparticles themselves. Microscale Acoustofluidics provides an introduction to the field providing the background to the fundamental physics including chapters on governing equations in microfluidics and perturbation theory and ultrasound resonances, acoustic radiation force on small particles, continuum mechanics for ultrasonic particle manipulation, and piezoelectricity and application to the excitation of acoustic fields for ultrasonic particle manipulation. The book also provides information on the design and characterization of ultrasonic particle manipulation devices as well as applications in acoustic trapping and immunoassays. Written by leading experts in the field, the book will appeal to postgraduate students and researchers interested in microfluidics and lab-on-a-chip applications.

Imaging Flow Cytometry

Author : Natasha S. Barteneva
Publisher : Humana
ISBN 13 : 9781493933006
Total Pages : 0 pages
Book Rating : 4.9/5 (33 download)

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Book Synopsis Imaging Flow Cytometry by : Natasha S. Barteneva

Download or read book Imaging Flow Cytometry written by Natasha S. Barteneva and published by Humana. This book was released on 2015-11-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Cellular Diagnostics

Author : Ulrich Sack
Publisher : Karger Medical and Scientific Publishers
ISBN 13 : 3805585551
Total Pages : 764 pages
Book Rating : 4.8/5 (55 download)

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Book Synopsis Cellular Diagnostics by : Ulrich Sack

Download or read book Cellular Diagnostics written by Ulrich Sack and published by Karger Medical and Scientific Publishers. This book was released on 2009-01-01 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the updated English version of the 2006 German bestseller Zellulare Diagnostik, a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.

Flow Cytometry and Cell Sorting

Author : Andreas Radbruch
Publisher : Springer Science & Business Media
ISBN 13 : 3662041294
Total Pages : 365 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Flow Cytometry and Cell Sorting by : Andreas Radbruch

Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Flow Cytometry

Author : Alice Longobardi Givan
Publisher : John Wiley & Sons
ISBN 13 : 1118688392
Total Pages : 309 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Flow Cytometry by : Alice Longobardi Givan

Download or read book Flow Cytometry written by Alice Longobardi Givan and published by John Wiley & Sons. This book was released on 2013-04-10 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Basic Science Methods for Clinical Researchers

Author : Morteza Jalali
Publisher : Academic Press
ISBN 13 : 012803078X
Total Pages : 383 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali

Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Diagnostic Hematology

Author : Norman Beck
Publisher : Springer Science & Business Media
ISBN 13 : 1848002955
Total Pages : 504 pages
Book Rating : 4.8/5 (48 download)

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Book Synopsis Diagnostic Hematology by : Norman Beck

Download or read book Diagnostic Hematology written by Norman Beck and published by Springer Science & Business Media. This book was released on 2008-12-19 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: The approach described in this book is different from that in most student texts, and has been very successful in practice, starting almost from scratch, but omitting many of the ‘basics’ such as the details of hematopoiesis, laboratory technology, and so on, which are hardly relevant to the practising clinician and student in the wards, and are primarily of interest to the hematologist and sometimes to the clinical specialist. Considerable emphasis is given to the clinical history and examination, and the interpretation of the clinical patterns thus exposed. Hopefully it will overcome many of the traditional problems experienced in practical diagnostic haematology.

Single-cell Sequencing and Methylation

Author : Buwei Yu
Publisher : Springer
ISBN 13 : 9789811544934
Total Pages : 247 pages
Book Rating : 4.5/5 (449 download)

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Book Synopsis Single-cell Sequencing and Methylation by : Buwei Yu

Download or read book Single-cell Sequencing and Methylation written by Buwei Yu and published by Springer. This book was released on 2020-10-14 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the rapid development of biotechnologies, single-cell sequencing has become an important tool for understanding the molecular mechanisms of diseases, defining cellular heterogeneities and characteristics, and identifying intercellular communications and single-cell-based biomarkers. Providing a clear overview of the clinical applications, the book presents state-of-the-art information on immune cell function, cancer progression, infection, and inflammation gained from single-cell DNA or RNA sequencing. Furthermore, it explores the role of target gene methylation in the pathogenesis of diseases, with a focus on respiratory cancer, infection and chronic diseases. As such it is a valuable resource for clinical researchers and physicians, allowing them to refresh their knowledge and improve early diagnosis and therapy for patients.

Nanotechnology in Medicine

Author : Vishnu Kirthi Arivarasan
Publisher : Springer Nature
ISBN 13 : 3030610217
Total Pages : 274 pages
Book Rating : 4.0/5 (36 download)

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Book Synopsis Nanotechnology in Medicine by : Vishnu Kirthi Arivarasan

Download or read book Nanotechnology in Medicine written by Vishnu Kirthi Arivarasan and published by Springer Nature. This book was released on 2021-01-21 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomedicine is the field of science that deals with organic applications of medicine at the nano-scale level. It primarily addresses finding, anticipating, and treating sickness, as well as using nanotechnology to assist in controlling human frameworks at the cellular level. The nature of nanotechnology allows it to address numerous medical issues in humans. This book offers comprehensive information to better comprehend and apply multifunctional nanoparticles in nanomedicine, and thus open avenues in the field. Medicating at the nanolevel is an exceptional therapeutic avenue, as it avoids symptoms associated with conventional medicines. This book investigates recent insights into structuring novel drug delivery frameworks. It concentrates on the physical characteristics of drug delivery transporters, and the preliminary procedures involved in their use. The book offers in-depth detail that benefits academics and researchers alike, containing broad research from experts in the field, and serves as a guide for students and researchers in the field of nanomedicine, drug delivery, and nanotechnology.

Micro- and Nanofluidics for Bionanoparticle Analysis

Author : Yong Zeng
Publisher : MDPI
ISBN 13 : 3039215949
Total Pages : 138 pages
Book Rating : 4.0/5 (392 download)

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Book Synopsis Micro- and Nanofluidics for Bionanoparticle Analysis by : Yong Zeng

Download or read book Micro- and Nanofluidics for Bionanoparticle Analysis written by Yong Zeng and published by MDPI. This book was released on 2019-10-16 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bionanoparticles such as microorganisms and exosomes are recoganized as important targets for clinical applications, food safety, and environmental monitoring. Other nanoscale biological particles, includeing liposomes, micelles, and functionalized polymeric particles are widely used in nanomedicines. The recent deveopment of microfluidic and nanofluidic technologies has enabled the separation and anslysis of these species in a lab-on-a-chip platform, while there are still many challenges to address before these analytical tools can be adopted in practice. For example, the complex matrices within which these species reside in create a high background for their detection. Their small dimension and often low concentration demand creative strategies to amplify the sensing signal and enhance the detection speed. This Special Issue aims to recruit recent discoveries and developments of micro- and nanofluidic strategies for the processing and analysis of biological nanoparticles. The collection of papers will hopefully bring out more innovative ideas and fundamental insights to overcome the hurdles faced in the separation and detection of bionanoparticles.

Circulating Tumor Cells

Author : Mark Jesus M. Magbanua
Publisher : Humana Press
ISBN 13 : 9781493971435
Total Pages : 306 pages
Book Rating : 4.9/5 (714 download)

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Book Synopsis Circulating Tumor Cells by : Mark Jesus M. Magbanua

Download or read book Circulating Tumor Cells written by Mark Jesus M. Magbanua and published by Humana Press. This book was released on 2017-09-15 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Circula ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.

Molecular Genetic Pathology

Author : Liang Cheng
Publisher : Springer
ISBN 13 : 9781461447993
Total Pages : 1136 pages
Book Rating : 4.4/5 (479 download)

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Book Synopsis Molecular Genetic Pathology by : Liang Cheng

Download or read book Molecular Genetic Pathology written by Liang Cheng and published by Springer. This book was released on 2013-03-05 with total page 1136 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Genetic Pathology, Second Edition presents up-to-date material containing fundamental information relevant to the clinical practice of molecular genetic pathology. Fully updated in each area and expanded to include identification of new infectious agents (H1N1), new diagnostic biomarkers and biomarkers for targeted cancer therapy. This edition is also expanded to include the many new technologies that have become available in the past few years such as microarray (AmpliChip) and high throughput deep sequencing, which will certainly change the clinical practice of molecular genetic pathology. Part I examines the clinical aspects of molecular biology and technology, genomics. Poharmacogenomics and proteomics, while Part II covers the clinically relevant information of medical genetics, hematology, transfusion medicine, oncology, and forensic pathology. Supplemented with many useful figures and presented in a helpful bullet-point format, Molecular Genetic Pathology, Second Edition provides a unique reference for practicing pathologists, oncologists, internists, and medical genetisists. Furthermore, a book with concise overview of the field and highlights of clinical applications will certainly help those trainees, including pathology residents, genetics residents, molecular pathology fellows, internists, hematology/oncology fellows, and medical technologists in preparing for their board examination/certification.

Micro/Nano Devices for Blood Analysis

Author : Rui A. Lima
Publisher : MDPI
ISBN 13 : 3039218247
Total Pages : 174 pages
Book Rating : 4.0/5 (392 download)

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Book Synopsis Micro/Nano Devices for Blood Analysis by : Rui A. Lima

Download or read book Micro/Nano Devices for Blood Analysis written by Rui A. Lima and published by MDPI. This book was released on 2019-12-03 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of micro- and nanodevices for blood analysis is an interdisciplinary subject that demands the integration of several research fields, such as biotechnology, medicine, chemistry, informatics, optics, electronics, mechanics, and micro/nanotechnologies. Over the last few decades, there has been a notably fast development in the miniaturization of mechanical microdevices, later known as microelectromechanical systems (MEMS), which combine electrical and mechanical components at a microscale level. The integration of microflow and optical components in MEMS microdevices, as well as the development of micropumps and microvalves, have promoted the interest of several research fields dealing with fluid flow and transport phenomena happening in microscale devices. Microfluidic systems have many advantages over their macroscale counterparts, offering the ability to work with small sample volumes, providing good manipulation and control of samples, decreasing reaction times, and allowing parallel operations in one single step. As a consequence, microdevices offer great potential for the development of portable and point-of-care diagnostic devices, particularly for blood analysis. Moreover, the recent progress in nanotechnology has contributed to its increasing popularity, and has expanded the areas of application of microfluidic devices, including in the manipulation and analysis of flows on the scale of DNA, proteins, and nanoparticles (nanoflows). In this Special Issue, we invited contributions (original research papers, review articles, and brief communications) that focus on the latest advances and challenges in micro- and nanodevices for diagnostics and blood analysis, micro- and nanofluidics, technologies for flow visualization, MEMS, biochips, and lab-on-a-chip devices and their application to research and industry. We hope to provide an opportunity to the engineering and biomedical community to exchange knowledge and information and to bring together researchers who are interested in the general field of MEMS and micro/nanofluidics and, especially, in its applications to biomedical areas.

Chemosensitivity

Author : Rosalyn D. Blumenthal
Publisher : Humana Press
ISBN 13 : 9781588295866
Total Pages : 442 pages
Book Rating : 4.2/5 (958 download)

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Book Synopsis Chemosensitivity by : Rosalyn D. Blumenthal

Download or read book Chemosensitivity written by Rosalyn D. Blumenthal and published by Humana Press. This book was released on 2005-02-14 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity,Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains cutting-edge protocols for classifying tumors into response categories and for customizing therapy to individuals. These readily reproducible techniques allow measurements of DNA damage, apoptotic cell death, and the molecular and cellular regulators of cytotoxicity, as well as in vivo animal modeling of chemosensitivity. A companion volume, Volume 1: In Vitro Assays contains in vitro and in vivo techniques to identify which new agents or combination of agents are effective for each type of tumor.

Microtechnology for Cell Manipulation and Sorting

Author : Wonhee Lee
Publisher : Springer
ISBN 13 : 3319441396
Total Pages : 287 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Microtechnology for Cell Manipulation and Sorting by : Wonhee Lee

Download or read book Microtechnology for Cell Manipulation and Sorting written by Wonhee Lee and published by Springer. This book was released on 2016-10-05 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.

Cancer Nanotechnology

Author :
Publisher : Academic Press
ISBN 13 : 0128141700
Total Pages : 224 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Cancer Nanotechnology by :

Download or read book Cancer Nanotechnology written by and published by Academic Press. This book was released on 2018-06-23 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Cancer Research, Volume 139, provides invaluable information on the exciting and fast-moving field of cancer research. Original reviews are presented on a variety of topics relating to the rapidly developing intersection between nanotechnology and cancer research, with unique sections in the new release focusing on Exosomes as a theranostic for lung cancer, Nanotechnology and cancer immunotherapy, Ultrasound imaging agents and delivery systems, Dendronized systems for the delivery of chemotherapeutics, Thermosensitive liposomes for image-guided drug delivery, Supramolecular Chemistry in Tumor Analysis and Drug Delivery, Gold nanoparticles for delivery of cancer therapeutics, and Single cell barcode microchip for cancer research and therapy. - Provides the latest information on cancer research - Offers outstanding and original reviews on a range of cancer research topics - Serves as an indispensable reference for researchers and students alike

Practical Flow Cytometry

Author : Howard M. Shapiro
Publisher : John Wiley & Sons
ISBN 13 : 0471434035
Total Pages : 736 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Practical Flow Cytometry by : Howard M. Shapiro

Download or read book Practical Flow Cytometry written by Howard M. Shapiro and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.