Cryo Negative Staining

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Electron Microscopy Methods and Protocols

Author : M. A. Nasser Hajibagheri
Publisher : Springer Science & Business Media
ISBN 13 : 1592592015
Total Pages : 292 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri

Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Cryo-negative Staining

Author : Sacha De Carlo
Publisher :
ISBN 13 :
Total Pages : 122 pages
Book Rating : 4.:/5 (717 download)

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Book Synopsis Cryo-negative Staining by : Sacha De Carlo

Download or read book Cryo-negative Staining written by Sacha De Carlo and published by . This book was released on 2002 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Biomolecular and Bioanalytical Techniques

Author : Vasudevan Ramesh
Publisher : John Wiley & Sons
ISBN 13 : 1119483964
Total Pages : 576 pages
Book Rating : 4.1/5 (194 download)

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Book Synopsis Biomolecular and Bioanalytical Techniques by : Vasudevan Ramesh

Download or read book Biomolecular and Bioanalytical Techniques written by Vasudevan Ramesh and published by John Wiley & Sons. This book was released on 2019-06-10 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.

NEGATIVE STAINING CRYOELECTRON

Author : J. HARRIS
Publisher : Taylor & Francis
ISBN 13 :
Total Pages : 232 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis NEGATIVE STAINING CRYOELECTRON by : J. HARRIS

Download or read book NEGATIVE STAINING CRYOELECTRON written by J. HARRIS and published by Taylor & Francis. This book was released on 1997 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the start of biological studies using the transmission electron microscope scientists have sought to develop procedures for the preparation and investigation of the thinly spread specimens of biological particulates. Negative Staining and Cryoelect

cryoEM

Author : Tamir Gonen
Publisher : Humana
ISBN 13 : 9781071609682
Total Pages : 350 pages
Book Rating : 4.6/5 (96 download)

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Book Synopsis cryoEM by : Tamir Gonen

Download or read book cryoEM written by Tamir Gonen and published by Humana. This book was released on 2021-12-28 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology.

Negative Staining

Author : M. A. Hayat
Publisher : McGraw-Hill Companies
ISBN 13 :
Total Pages : 280 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Negative Staining by : M. A. Hayat

Download or read book Negative Staining written by M. A. Hayat and published by McGraw-Hill Companies. This book was released on 1990 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Single-particle Cryo-electron Microscopy

Author : Joachim Frank
Publisher : World Scientific Publishing Company
ISBN 13 : 9789813234857
Total Pages : 0 pages
Book Rating : 4.2/5 (348 download)

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Book Synopsis Single-particle Cryo-electron Microscopy by : Joachim Frank

Download or read book Single-particle Cryo-electron Microscopy written by Joachim Frank and published by World Scientific Publishing Company. This book was released on 2017-12-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as important results obtained by this technique in the study of biological macromolecules and their interactions. Importantly, neither symmetries nor ordered arrangements (as in two-dimensional crystals, helical assemblies, icosahedral viruses) are required. Although the biological applications are mainly in the area of ribosome structure and function, the elucidation of membrane channel structures and their activation and gating mechanisms are represented, as well. The book is introduced by a commentary that explains the original development of concepts, describes the contributions of the author's colleagues and students, and shows how challenges were overcome as the technique matured. Along the way, the ribosome served as an example for a macromolecule with intricate structure and conformational dynamics that pose challenges for three-dimensional visualization. Toward the end of the book -- bringing us to the present time -- molecular structures with near-atomic resolution are presented, and a novel type of computational analysis, manifold embedding, is introduced. Single-particle cryo-EM is currently revolutionizing structural biology, presenting a powerful alternative to X-ray crystallography as a means to solve the structure of biological macromolecules. The book presents in one place a number of articles containing key advances in mathematical and computational methods leading up to the present time. Secondly, the development of the technique over the years is reflected by ever-expanding discoveries in the field of ribosome structure and function. Thirdly, as all histories of ideas, the history of concepts pertaining to this new method of visualization is fascinating all in itself.

Cryo-EM Part A: Sample Preparation and Data Collection

Author :
Publisher : Academic Press
ISBN 13 : 0080956955
Total Pages : 466 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Cryo-EM Part A: Sample Preparation and Data Collection by :

Download or read book Cryo-EM Part A: Sample Preparation and Data Collection written by and published by Academic Press. This book was released on 2010-09-30 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide

Negative Staining and Cryoelectron Microscopy

Author : 0 J.R. Harris
Publisher :
ISBN 13 : 9781003423799
Total Pages : 0 pages
Book Rating : 4.4/5 (237 download)

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Book Synopsis Negative Staining and Cryoelectron Microscopy by : 0 J.R. Harris

Download or read book Negative Staining and Cryoelectron Microscopy written by 0 J.R. Harris and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the start of biological studies using the transmission electron microscope scientists have sought to develop procedures for the preparation and investigation of the thinly spread specimens of biological particulates. Negative Staining and Cryoelect

Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

Author : Alexander D. Hyatt
Publisher : CRC Press
ISBN 13 : 9780849367595
Total Pages : 476 pages
Book Rating : 4.3/5 (675 download)

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Book Synopsis Immuno-Gold Electron Microscopy in Virus Diagnosis and Research by : Alexander D. Hyatt

Download or read book Immuno-Gold Electron Microscopy in Virus Diagnosis and Research written by Alexander D. Hyatt and published by CRC Press. This book was released on 1992-11-10 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Diagnostic Electron Microscopy

Author : John Stirling
Publisher : John Wiley & Sons
ISBN 13 : 1119973996
Total Pages : 492 pages
Book Rating : 4.1/5 (199 download)

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Book Synopsis Diagnostic Electron Microscopy by : John Stirling

Download or read book Diagnostic Electron Microscopy written by John Stirling and published by John Wiley & Sons. This book was released on 2013-01-22 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Electron Microscopy Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.

Three-Dimensional Electron Microscopy of Macromolecular Assemblies

Author : Frank Joachim
Publisher : Elsevier
ISBN 13 : 0080525814
Total Pages : 361 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Three-Dimensional Electron Microscopy of Macromolecular Assemblies by : Frank Joachim

Download or read book Three-Dimensional Electron Microscopy of Macromolecular Assemblies written by Frank Joachim and published by Elsevier. This book was released on 1996-01-24 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized* Describes methods that have been instrumental in exploring the three-dimensional structure of* the nuclear pore complex* the calcium release channel;* the ribosome* chaperonins

Structural Biology in Drug Discovery

Author : Jean-Paul Renaud
Publisher : John Wiley & Sons
ISBN 13 : 1118900502
Total Pages : 1367 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis Structural Biology in Drug Discovery by : Jean-Paul Renaud

Download or read book Structural Biology in Drug Discovery written by Jean-Paul Renaud and published by John Wiley & Sons. This book was released on 2020-01-09 with total page 1367 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the most comprehensive and up-to-date overview of structure-based drug discovery covering both experimental and computational approaches, Structural Biology in Drug Discovery: Methods, Techniques, and Practices describes principles, methods, applications, and emerging paradigms of structural biology as a tool for more efficient drug development. Coverage includes successful examples, academic and industry insights, novel concepts, and advances in a rapidly evolving field. The combined chapters, by authors writing from the frontlines of structural biology and drug discovery, give readers a valuable reference and resource that: Presents the benefits, limitations, and potentiality of major techniques in the field such as X-ray crystallography, NMR, neutron crystallography, cryo-EM, mass spectrometry and other biophysical techniques, and computational structural biology Includes detailed chapters on druggability, allostery, complementary use of thermodynamic and kinetic information, and powerful approaches such as structural chemogenomics and fragment-based drug design Emphasizes the need for the in-depth biophysical characterization of protein targets as well as of therapeutic proteins, and for a thorough quality assessment of experimental structures Illustrates advances in the field of established therapeutic targets like kinases, serine proteinases, GPCRs, and epigenetic proteins, and of more challenging ones like protein-protein interactions and intrinsically disordered proteins

Electron Microscopy in Microbiology

Author :
Publisher : Academic Press
ISBN 13 : 0080860494
Total Pages : 443 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Electron Microscopy in Microbiology by :

Download or read book Electron Microscopy in Microbiology written by and published by Academic Press. This book was released on 1988-10-01 with total page 443 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of this acclaimed series deals with electron microscopic techniques applied for the elucidation of microbial structures and structure-function relationships at cellular, sub-cellular, and macromolecular levels. Many of the recent findings on ultrastructural features of microorganisms have been obtained with newly developed methods, though classical approaches have not lost their validity. Therefore, both conventional and new methods have been incorporated into this volume. The topics dealt with are meaningful not only in bacterial cytology but also in physiology, enzymology, biochemistry, and molecular biology, and include aspects of medical and biotechnological application.

Lipid-Protein Interactions

Author : Jörg H. Kleinschmidt
Publisher : Humana Press
ISBN 13 : 9781627032742
Total Pages : 0 pages
Book Rating : 4.0/5 (327 download)

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Book Synopsis Lipid-Protein Interactions by : Jörg H. Kleinschmidt

Download or read book Lipid-Protein Interactions written by Jörg H. Kleinschmidt and published by Humana Press. This book was released on 2013-02-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins with lipids. Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties. The methods described here are all actively used, complementary, and necessary to obtain comprehensive information about membrane structure and function. They include label-free approaches, imaging techniques and spectroscopic methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Lipid-Protein Interactions: Methods and Protocols seeks to serve both professional and novices with its wide range of the methods frequently used in this area of research.

Electron Microscopy

Author : John J. Bozzola
Publisher : Jones & Bartlett Learning
ISBN 13 : 9780763701925
Total Pages : 702 pages
Book Rating : 4.7/5 (19 download)

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Book Synopsis Electron Microscopy by : John J. Bozzola

Download or read book Electron Microscopy written by John J. Bozzola and published by Jones & Bartlett Learning. This book was released on 1999 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR

Structure Determination of Two Pore Forming Toxins by Cryo-negative Staining and Single Particle Three-dimensional Reconstruction

Download Structure Determination of Two Pore Forming Toxins by Cryo-negative Staining and Single Particle Three-dimensional Reconstruction PDF Online Free

Author : Catherine El-Bez
Publisher :
ISBN 13 :
Total Pages : 129 pages
Book Rating : 4.:/5 (853 download)

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Book Synopsis Structure Determination of Two Pore Forming Toxins by Cryo-negative Staining and Single Particle Three-dimensional Reconstruction by : Catherine El-Bez

Download or read book Structure Determination of Two Pore Forming Toxins by Cryo-negative Staining and Single Particle Three-dimensional Reconstruction written by Catherine El-Bez and published by . This book was released on 2004 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt: