Coupling Liquid Chromatography To Capillary Zone Electrophoresis Tandem Mass Spectrometry For Deep Top Down Proteomics

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Capillary Electrophoresis-Mass Spectrometry

Author : Christian Neusüß
Publisher : Springer Nature
ISBN 13 : 1071624938
Total Pages : 264 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Capillary Electrophoresis-Mass Spectrometry by : Christian Neusüß

Download or read book Capillary Electrophoresis-Mass Spectrometry written by Christian Neusüß and published by Springer Nature. This book was released on 2022-08-08 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.

Capillary Electrophoresis - Mass Spectrometry (CE-MS)

Author : Gerhardus de Jong
Publisher : John Wiley & Sons
ISBN 13 : 3527339248
Total Pages : 364 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Capillary Electrophoresis - Mass Spectrometry (CE-MS) by : Gerhardus de Jong

Download or read book Capillary Electrophoresis - Mass Spectrometry (CE-MS) written by Gerhardus de Jong and published by John Wiley & Sons. This book was released on 2016-09-13 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diese Monographie bietet einen vollständigen Überblick über die Prinzipien und Anwendungen der Kapillarelektrophorese (CE) und der Massenspektrometrie (MS) und legt den Nachdruck insbesondere auf Kopplungsschnittstellen. Ausführlich erläutert werden auch alle relevanten Substanzklassen. Ein einzigartiges Wissenskompendium für alle, die sich CE-MS beschäftigen!

Fundamentals and Applications of Fourier Transform Mass Spectrometry

Author : Philippe Schmitt-Kopplin
Publisher : Elsevier
ISBN 13 : 0128140143
Total Pages : 780 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Fundamentals and Applications of Fourier Transform Mass Spectrometry by : Philippe Schmitt-Kopplin

Download or read book Fundamentals and Applications of Fourier Transform Mass Spectrometry written by Philippe Schmitt-Kopplin and published by Elsevier. This book was released on 2019-08-11 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals and Applications of Fourier Transform Mass Spectrometry is the first book to delve into the underlying principles on the topic and their linkage to industrial applications. Drs. Schmitt-Kopplin and Kanawati have brought together a team of leading experts in their respective fields to present this technique from many different perspectives, describing, at length, the pros and cons of FT-ICR and Orbitrap. Numerous examples help researchers decide which instruments to use for their particular scientific problem and which data analysis methods should be applied to get the most out of their data. - Covers FT-ICR-MS and Orbitrap's fundamentals, enhancing researcher knowledge - Includes details on ion sources, data processing, chemical analysis and imaging - Provides examples across the wide spectrum of applications, including omics, environmental, chemical, pharmaceutical and food analysis

Proteomics Sample Preparation

Author : Jörg von Hagen
Publisher : John Wiley & Sons
ISBN 13 : 3527644695
Total Pages : 498 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Proteomics Sample Preparation by : Jörg von Hagen

Download or read book Proteomics Sample Preparation written by Jörg von Hagen and published by John Wiley & Sons. This book was released on 2011-08-24 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.

Proteoform Identification

Author : Liangliang Sun
Publisher : Humana
ISBN 13 : 9781071623244
Total Pages : 240 pages
Book Rating : 4.6/5 (232 download)

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Book Synopsis Proteoform Identification by : Liangliang Sun

Download or read book Proteoform Identification written by Liangliang Sun and published by Humana. This book was released on 2022-07-04 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.

Capillary Gel Electrophoresis

Author : Andras Guttman
Publisher : Newnes
ISBN 13 : 0080931359
Total Pages : 391 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Manual of Cardiovascular Proteomics

Author : Giulio Agnetti
Publisher : Springer
ISBN 13 : 3319318284
Total Pages : 442 pages
Book Rating : 4.3/5 (193 download)

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Book Synopsis Manual of Cardiovascular Proteomics by : Giulio Agnetti

Download or read book Manual of Cardiovascular Proteomics written by Giulio Agnetti and published by Springer. This book was released on 2016-09-15 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book fulfils the need to keep up with the high number of innovations in proteomics, and at the same time to warn the readers about the danger of manufacturers and scientists claims around new technologies. Mass spectrometry stands as the core technology in proteomics. The emerging field of targeted proteomics and its potential applications in the cardiovascular arena are also reviewed and discussed. A concluding section highilghts the promise of proteomics in the light of these recent developments. As this technique and its applications have undergone remarkable advances in the past years, recent updates on proteomic applications are covered. Another key concept revealed by proteomic technologies is that the extent of protein post-translational modifications (PTMs) as well as their impact on the phenotype has been underestimated by pre-proteomics science. As such, part of the manual focuses on the emerging role of PTMs in basic cardiovascular sciences and in the clinics. In fact, there is an emerging consensus that the detailed annotation of protein PTMs could lead to a more in-depth representation of biological systems, translating into more specific targets for therapy as well as biomarkers. Moreover, a recent trend is so-called “targeted proteomics”. The approach was awarded the title of “Method of the Year” by Nature in 2013 (see the editorial by Vivien Marx in 1st issue of Nature in Jan 2013). According to a few proteomic scientists the emphasis should not be placed on generating long lists of proteins but lists of proteins with a true biological meaning.

Principles and Applications of Clinical Mass Spectrometry

Author : Nader Rifai
Publisher : Elsevier
ISBN 13 : 0128160640
Total Pages : 218 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Principles and Applications of Clinical Mass Spectrometry by : Nader Rifai

Download or read book Principles and Applications of Clinical Mass Spectrometry written by Nader Rifai and published by Elsevier. This book was released on 2018-06-26 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is a concise resource for quick implementation of mass spectrometry methods in clinical laboratory work. Focusing on the practical use of these techniques, the first half of the book covers principles of chromatographic separations, principles and types of mass spectrometers, and sample preparation for analysis; the second half outlines the main applications of this technology within clinical laboratory settings, including determination of small molecules and peptides, as well as pathogen identification. A thorough yet succinct guide to using mass spectrometry technology in the clinical laboratory, Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is an essential resource for chemists, pharmaceutical and biotech researchers, certain government agencies, and standardization groups. - Provides concrete examples of the main applications of mass spectrometry technology - Describes current capabilities of the LC- and MS-based analytical methods - Details methods for successful analytical work in the field

Capillary Zone Electrophoresis

Author : František Foret
Publisher :
ISBN 13 :
Total Pages : 368 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Capillary Zone Electrophoresis by : František Foret

Download or read book Capillary Zone Electrophoresis written by František Foret and published by . This book was released on 1993 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fundamentals of Contemporary Mass Spectrometry

Author : Chhabil Dass
Publisher : John Wiley & Sons
ISBN 13 : 0470118482
Total Pages : 512 pages
Book Rating : 4.4/5 (71 download)

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Book Synopsis Fundamentals of Contemporary Mass Spectrometry by : Chhabil Dass

Download or read book Fundamentals of Contemporary Mass Spectrometry written by Chhabil Dass and published by John Wiley & Sons. This book was released on 2007-05-11 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern mass spectrometry - the instrumentation and applications in diverse fields Mass spectrometry has played a pivotal role in a variety of scientific disciplines. Today it is an integral part of proteomics and drug discovery process. Fundamentals of Contemporary Mass Spectrometry gives readers a concise and authoritative overview of modern mass spectrometry instrumentation, techniques, and applications, including the latest developments. After an introduction to the history of mass spectrometry and the basic underlying concepts, it covers: Instrumentation, including modes of ionization, condensed phase ionization techniques, mass analysis and ion detection, tandem mass spectrometry, and hyphenated separation techniques Organic and inorganic mass spectrometry Biological mass spectrometry, including the analysis of proteins and peptides, oligosaccharides, lipids, oligonucleotides, and other biological materials Applications to quantitative analysis Based on proven teaching principles, each chapter is complete with a concise overview, highlighted key points, practice exercises, and references to additional resources. Hints and solutions to the exercises are provided in an appendix. To facilitate learning and improve problem-solving skills, several worked-out examples are included. This is a great textbook for graduate students in chemistry, and a robust, practical resource for researchers and scientists, professors, laboratory managers, technicians, and others. It gives scientists in diverse disciplines a practical foundation in modern mass spectrometry.

Chemical Biomarkers in Aquatic Ecosystems

Author : Thomas S. Bianchi
Publisher : Princeton University Press
ISBN 13 : 1400839106
Total Pages : 417 pages
Book Rating : 4.4/5 (8 download)

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Book Synopsis Chemical Biomarkers in Aquatic Ecosystems by : Thomas S. Bianchi

Download or read book Chemical Biomarkers in Aquatic Ecosystems written by Thomas S. Bianchi and published by Princeton University Press. This book was released on 2011-02-28 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook provides a unique and thorough look at the application of chemical biomarkers to aquatic ecosystems. Defining a chemical biomarker as a compound that can be linked to particular sources of organic matter identified in the sediment record, the book indicates that the application of these biomarkers for an understanding of aquatic ecosystems consists of a biogeochemical approach that has been quite successful but underused. This book offers a wide-ranging guide to the broad diversity of these chemical biomarkers, is the first to be structured around the compounds themselves, and examines them in a connected and comprehensive way. This timely book is appropriate for advanced undergraduate and graduate students seeking training in this area; researchers in biochemistry, organic geochemistry, and biogeochemistry; researchers working on aspects of organic cycling in aquatic ecosystems; and paleoceanographers, petroleum geologists, and ecologists. Provides a guide to the broad diversity of chemical biomarkers in aquatic environments The first textbook to be structured around the compounds themselves Describes the structure, biochemical synthesis, analysis, and reactivity of each class of biomarkers Offers a selection of relevant applications to aquatic systems, including lakes, rivers, estuaries, oceans, and paleoenvironments Demonstrates the utility of using organic molecules as tracers of processes occurring in aquatic ecosystems, both modern and ancient

Separation Techniques Applied to Omics Sciences

Author : Ana Valéria Colnaghi Simionato
Publisher : Springer Nature
ISBN 13 : 3030772527
Total Pages : 277 pages
Book Rating : 4.0/5 (37 download)

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Book Synopsis Separation Techniques Applied to Omics Sciences by : Ana Valéria Colnaghi Simionato

Download or read book Separation Techniques Applied to Omics Sciences written by Ana Valéria Colnaghi Simionato and published by Springer Nature. This book was released on 2021-10-09 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.

Handbook of Surface Plasmon Resonance

Author : Richard B. M. Schasfoort
Publisher : Royal Society of Chemistry
ISBN 13 : 1782627308
Total Pages : 555 pages
Book Rating : 4.7/5 (826 download)

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Book Synopsis Handbook of Surface Plasmon Resonance by : Richard B. M. Schasfoort

Download or read book Handbook of Surface Plasmon Resonance written by Richard B. M. Schasfoort and published by Royal Society of Chemistry. This book was released on 2017-05-30 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events, this book provides a total system description including optics, fluidics and sensor surfaces for a wide researcher audience.

HPLC and UHPLC for Practicing Scientists

Author : Michael W. Dong
Publisher : John Wiley & Sons
ISBN 13 : 1119313767
Total Pages : 410 pages
Book Rating : 4.1/5 (193 download)

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Book Synopsis HPLC and UHPLC for Practicing Scientists by : Michael W. Dong

Download or read book HPLC and UHPLC for Practicing Scientists written by Michael W. Dong and published by John Wiley & Sons. This book was released on 2019-07-23 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.

Proteomics in Practice

Author : Reiner Westermeier
Publisher : John Wiley & Sons
ISBN 13 : 3527622306
Total Pages : 502 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Proteomics in Practice by : Reiner Westermeier

Download or read book Proteomics in Practice written by Reiner Westermeier and published by John Wiley & Sons. This book was released on 2008-09-08 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

Antibody Glycosylation

Author : Marija Pezer
Publisher : Springer Nature
ISBN 13 : 3030769127
Total Pages : 588 pages
Book Rating : 4.0/5 (37 download)

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Book Synopsis Antibody Glycosylation by : Marija Pezer

Download or read book Antibody Glycosylation written by Marija Pezer and published by Springer Nature. This book was released on 2021-10-22 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes recent advances in antibody glycosylation research. Covering major topics relevant for immunoglobulin glycosylation - analytical methods, biosynthesis and regulation, modulation of effector functions - it provides new perspectives for research and development in the field of therapeutic antibodies, biomarkers, vaccinations, and immunotherapy. Glycans attached to both variable and constant regions of antibodies are known to affect the antibody conformation, stability, and effector functions. Although it focuses on immunoglobulin G (IgG), the most explored antibody in this context, and unravels the natural phenomena resulting from the mixture of IgG glycovariants present in the human body, the book also discusses other classes of human immunoglobulins, as well as immunoglobulins produced in other species and production systems. Further, it reviews the glycoanalytical methods applied to antibodies and addresses a range of less commonly explored topics, such as automatization and bioinformatics aspects of high-throughput antibody glycosylation analysis. Lastly, the book highlights application areas ranging from the ones already benefitting from antibody glycoengineering (such as monoclonal antibody production), to those still in the research stages (such as exploration of antibody glycosylation as a clinical or biological age biomarker), and the potential use of antibody glycosylation in the optimization of vaccine production and immunization protocols. Summarizing the current knowledge on the broad topic of antibody glycosylation and its therapeutic and biomarker potential, this book will appeal to a wide biomedical readership in academia and industry alike. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Principles and Applications of Molecular Diagnostics

Author : Nader Rifai
Publisher : Elsevier
ISBN 13 : 0128160624
Total Pages : 424 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Principles and Applications of Molecular Diagnostics by : Nader Rifai

Download or read book Principles and Applications of Molecular Diagnostics written by Nader Rifai and published by Elsevier. This book was released on 2018-06-13 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. - Explains the principles and tools of molecular biology - Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results - Provides a detailed description of current molecular applications used to solve diagnostics tasks