Counting Molecules Within Cells

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Counting Molecules Within Cells

Author : Valerie Coffman
Publisher : Biota Publishing
ISBN 13 : 1615046550
Total Pages : 76 pages
Book Rating : 4.6/5 (15 download)

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Book Synopsis Counting Molecules Within Cells by : Valerie Coffman

Download or read book Counting Molecules Within Cells written by Valerie Coffman and published by Biota Publishing. This book was released on 2014-10-01 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt: A fundamental component of quantitative cell biology is the ability to count molecules within cells. The numbers of molecules and stoichiometries are the basis for structural models of protein complexes and simulations of biological processes. A variety of methods exist for in vivo quantifications, but the focus of this volume is mainly on fluorescence methods. The two most popular methods are stepwise photobleaching and ratio comparison using a standard curve. With recent advances in genome editing techniques, most model organisms are amenable to inserting coding sequences for fluorescent proteins into native genetic loci, making quantification of proteins by fluorescence microscopy one of the most ubiquitous tools available to cell biologists. The acquisition and analysis methods range from simple to complex, and most have been validated by counting with multiple methods and other types of data. Researchers should be aware of sources of error in the acquisition and analysis, but the accuracy of these methods is high. Quantification by fluorescence microscopy has yielded valuable new insights into many aspects of cell biology, highlighting its place among the standard tools for molecular and cell biologists.

Cell Biology by the Numbers

Author : Ron Milo
Publisher : Garland Science
ISBN 13 : 1317230698
Total Pages : 400 pages
Book Rating : 4.3/5 (172 download)

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Book Synopsis Cell Biology by the Numbers by : Ron Milo

Download or read book Cell Biology by the Numbers written by Ron Milo and published by Garland Science. This book was released on 2015-12-07 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid

Molecular Biology of The Cell

Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of The Cell by : Bruce Alberts

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Concepts of Biology

Author : Samantha Fowler
Publisher :
ISBN 13 : 9789888407453
Total Pages : 618 pages
Book Rating : 4.4/5 (74 download)

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Book Synopsis Concepts of Biology by : Samantha Fowler

Download or read book Concepts of Biology written by Samantha Fowler and published by . This book was released on 2018-01-07 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.

Nanoscale Photonic Imaging

Author : Tim Salditt
Publisher : Springer Nature
ISBN 13 : 3030344134
Total Pages : 634 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis Nanoscale Photonic Imaging by : Tim Salditt

Download or read book Nanoscale Photonic Imaging written by Tim Salditt and published by Springer Nature. This book was released on 2020-06-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.

Cell Adhesion Molecules in Cancer and Inflammation

Author : Agamemnon A. Epenetos
Publisher : CRC Press
ISBN 13 : 9783718655168
Total Pages : 264 pages
Book Rating : 4.6/5 (551 download)

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Book Synopsis Cell Adhesion Molecules in Cancer and Inflammation by : Agamemnon A. Epenetos

Download or read book Cell Adhesion Molecules in Cancer and Inflammation written by Agamemnon A. Epenetos and published by CRC Press. This book was released on 1995-06-01 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Begins a series for clinicians, graduate students, and researchers in academia, regulatory agencies, or industry, offering monographs both on classes of adhesion molecules and on the function of such molecules in particular systems. Here the emphasis is on the clinical evaluation of these molecules, particularly as novel biochemical and genetic markers to define tumor differentiation based on functional and morphological assessment, and also their potential use as targets for biological therapy. Includes ten pages of color plates. Annotation copyright by Book News, Inc., Portland, OR

Minorities and Small Numbers from Molecules to Organisms in Biology

Author : Takeharu Nagai
Publisher : Springer
ISBN 13 : 9811320837
Total Pages : 150 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Minorities and Small Numbers from Molecules to Organisms in Biology by : Takeharu Nagai

Download or read book Minorities and Small Numbers from Molecules to Organisms in Biology written by Takeharu Nagai and published by Springer. This book was released on 2018-11-03 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an accessible introduction to an exciting new field of life science in which the focus is on small numbers of molecules and minorities within cell populations and their significance for the understanding of biological phenomena. Numbers, or quantitative data, are attracting more attention in cell biology following, for example, determination of the absolute copy number of each protein species in each bacterial cell and the recognition of leader cells that drive collective cell migration. Within this context, the authors present recent advances in experimental techniques, biological findings, and theories. A variety of cutting-edge topics and issues are addressed, with explanation of the ways in which recent developments in the field cast light on seemingly straightforward but difficult-to-answer questions. Readers will learn that we are on the verge of a paradigm shift as the importance of cooperation among groups of molecules in live cells is acknowledged. The book is designed to be enjoyable to read and easy to understand. It will be of interest for a wide range of readers, including young researchers and undergraduate/high school students.

Chemistry 2e

Author : Paul Flowers
Publisher :
ISBN 13 : 9781947172623
Total Pages : 0 pages
Book Rating : 4.1/5 (726 download)

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Book Synopsis Chemistry 2e by : Paul Flowers

Download or read book Chemistry 2e written by Paul Flowers and published by . This book was released on 2019-02-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemistry 2e is designed to meet the scope and sequence requirements of the two-semester general chemistry course. The textbook provides an important opportunity for students to learn the core concepts of chemistry and understand how those concepts apply to their lives and the world around them. The book also includes a number of innovative features, including interactive exercises and real-world applications, designed to enhance student learning. The second edition has been revised to incorporate clearer, more current, and more dynamic explanations, while maintaining the same organization as the first edition. Substantial improvements have been made in the figures, illustrations, and example exercises that support the text narrative. Changes made in Chemistry 2e are described in the preface to help instructors transition to the second edition.

Single-Molecule Cellular Biophysics

Author : Mark C. Leake
Publisher : Cambridge University Press
ISBN 13 : 1107005833
Total Pages : 291 pages
Book Rating : 4.1/5 (7 download)

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Book Synopsis Single-Molecule Cellular Biophysics by : Mark C. Leake

Download or read book Single-Molecule Cellular Biophysics written by Mark C. Leake and published by Cambridge University Press. This book was released on 2013-01-31 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Indispensable textbook for undergraduate students in the physical and life sciences, unravelling the inner workings of the cell.

Molecular Biology in Cellular Pathology

Author : John Crocker
Publisher : John Wiley & Sons
ISBN 13 : 0470864982
Total Pages : 400 pages
Book Rating : 4.4/5 (78 download)

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Book Synopsis Molecular Biology in Cellular Pathology by : John Crocker

Download or read book Molecular Biology in Cellular Pathology written by John Crocker and published by John Wiley & Sons. This book was released on 2003-09-12 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.

Methods in Molecular Biophysics

Author : Nathan R. Zaccai
Publisher : Cambridge University Press
ISBN 13 : 1108508804
Total Pages : 710 pages
Book Rating : 4.1/5 (85 download)

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Book Synopsis Methods in Molecular Biophysics by : Nathan R. Zaccai

Download or read book Methods in Molecular Biophysics written by Nathan R. Zaccai and published by Cambridge University Press. This book was released on 2017-05-18 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current techniques for studying biological macromolecules and their interactions are based on the application of physical methods, ranging from classical thermodynamics to more recently developed techniques for the detection and manipulation of single molecules. Reflecting the advances made in biophysics research over the past decade, and now including a new section on medical imaging, this new edition describes the physical methods used in modern biology. All key techniques are covered, including mass spectrometry, hydrodynamics, microscopy and imaging, diffraction and spectroscopy, electron microscopy, molecular dynamics simulations and nuclear magnetic resonance. Each method is explained in detail using examples of real-world applications. Short asides are provided throughout to ensure that explanations are accessible to life scientists, physicists and those with medical backgrounds. The book remains an unparalleled and comprehensive resource for graduate students of biophysics and medical physics in science and medical schools, as well as for research scientists looking for an introduction to techniques from across this interdisciplinary field.

Physical Principles in Sensing and Signaling

Author : Robert G. Endres
Publisher : OUP Oxford
ISBN 13 : 0191654280
Total Pages : 160 pages
Book Rating : 4.1/5 (916 download)

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Book Synopsis Physical Principles in Sensing and Signaling by : Robert G. Endres

Download or read book Physical Principles in Sensing and Signaling written by Robert G. Endres and published by OUP Oxford. This book was released on 2013-01-11 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although invisible to the bare eye, bacterial cells are large enough to make complex decisions. Cells are composed of thousands of different molecular species including DNA, proteins, and smaller molecules, allowing them to sense their environment, to process this information, and to respond accordingly. Such responses include expression of genes or the control of their movement. Despite these properties, a living cell exists in the physical world and follows its laws. Keeping this in mind can help answer questions such as how cells work and why they implement solutions to problems the way they do. Applying physical principles in biology allows researchers to solve challenging problems at the interface between biology and the physical sciences, including how accurately biological cells can sense chemicals in their environment, how cells encode physical stimuli in biochemical pathways, or how cells amplify signals and adapt to persistent stimulation. In this book, the reader is introduced to this fascinating topic without the need for extensive mathematical details or huge prior knowledge in biological physics.

Molecular Diagnostics

Author : George P. Patrinos
Publisher : Academic Press
ISBN 13 : 0128029889
Total Pages : 520 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Molecular Diagnostics by : George P. Patrinos

Download or read book Molecular Diagnostics written by George P. Patrinos and published by Academic Press. This book was released on 2016-10-27 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field

Calculations for Molecular Biology and Biotechnology

Author : Frank H. Stephenson
Publisher : Academic Press
ISBN 13 : 9780123756916
Total Pages : 460 pages
Book Rating : 4.7/5 (569 download)

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Book Synopsis Calculations for Molecular Biology and Biotechnology by : Frank H. Stephenson

Download or read book Calculations for Molecular Biology and Biotechnology written by Frank H. Stephenson and published by Academic Press. This book was released on 2010-07-30 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts

Neuroscience and Neurotechnology of Neuronal Cell Surface Molecules in Neural Circuits

Author : Masahito Yamagata
Publisher : Frontiers Media SA
ISBN 13 : 2889711498
Total Pages : 294 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Neuroscience and Neurotechnology of Neuronal Cell Surface Molecules in Neural Circuits by : Masahito Yamagata

Download or read book Neuroscience and Neurotechnology of Neuronal Cell Surface Molecules in Neural Circuits written by Masahito Yamagata and published by Frontiers Media SA. This book was released on 2021-08-05 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Cell adhesion molecules in neural development and disease

Author : Zsolt Lele
Publisher : Frontiers Media SA
ISBN 13 : 2832514219
Total Pages : 143 pages
Book Rating : 4.8/5 (325 download)

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Book Synopsis Cell adhesion molecules in neural development and disease by : Zsolt Lele

Download or read book Cell adhesion molecules in neural development and disease written by Zsolt Lele and published by Frontiers Media SA. This book was released on 2023-02-09 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Computer Simulation of Liquids

Author : Michael P. Allen
Publisher : Oxford University Press
ISBN 13 : 0192524704
Total Pages : 640 pages
Book Rating : 4.1/5 (925 download)

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Book Synopsis Computer Simulation of Liquids by : Michael P. Allen

Download or read book Computer Simulation of Liquids written by Michael P. Allen and published by Oxford University Press. This book was released on 2017-08-15 with total page 640 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a practical guide to molecular dynamics and Monte Carlo simulation techniques used in the modelling of simple and complex liquids. Computer simulation is an essential tool in studying the chemistry and physics of condensed matter, complementing and reinforcing both experiment and theory. Simulations provide detailed information about structure and dynamics, essential to understand the many fluid systems that play a key role in our daily lives: polymers, gels, colloidal suspensions, liquid crystals, biological membranes, and glasses. The second edition of this pioneering book aims to explain how simulation programs work, how to use them, and how to interpret the results, with examples of the latest research in this rapidly evolving field. Accompanying programs in Fortran and Python provide practical, hands-on, illustrations of the ideas in the text.