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Comparison Of Dig Nonradioacti
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Book Synopsis Nonradioactive Analysis of Biomolecules by : Christoph Kessler
Download or read book Nonradioactive Analysis of Biomolecules written by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids. The second edition of this successful laboratory manual describes in detail the highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation as well as PCR amplification. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques, enabling scientists to plan, design and conduct the appropriate procedures.
Book Synopsis Nonradioactive Labeling and Detection of Biomolecules by : Christoph Kessler
Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer. This book was released on 2012-12-06 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Book Synopsis Protocols for Nucleic Acid Analysis by Nonradioactive Probes by : Elena Hilario
Download or read book Protocols for Nucleic Acid Analysis by Nonradioactive Probes written by Elena Hilario and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Book Synopsis Embryonic Stem Cells by : Amita Sarkar
Download or read book Embryonic Stem Cells written by Amita Sarkar and published by Discovery Publishing House. This book was released on 2009 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis In Situ Hybridization Protocols by : Ian A. Darby
Download or read book In Situ Hybridization Protocols written by Ian A. Darby and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
Book Synopsis Molecular Histochemical Techniques by : Takehiko Koji
Download or read book Molecular Histochemical Techniques written by Takehiko Koji and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. It describes in situ nick translation and TUNEL in detail, along with radioactive and non-radioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. The methodology is set out in easy-to-follow language by leading scientists working in the field today. Both a rich source of the latest information and a practical lab manual.
Book Synopsis Plant Virology Protocols by : Gary D. Foster
Download or read book Plant Virology Protocols written by Gary D. Foster and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 557 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.
Book Synopsis Embryonic Stem Cells by : Kursad Turksen
Download or read book Embryonic Stem Cells written by Kursad Turksen and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is fair to say that embryonic stem (ES) cells have taken their place beside the human genome project as one of the most discussed biomedical issues of the day. It also seems certain that as this millennium unfolds we will see an increase in scientific and ethical debate about their potential utility in society. On the scientific front, it is clear that work on ES cells has already generated new possibilities and stimulated development of new strategies for increasing our und- standing of cell lineages and differentiation. It is not naïve to think that, within a decade or so, our overall understanding of stem cell biology will be as revolutionized as it was when the pioneering hemopoietic stem cell studies of Till and McCulloch in Toronto captured our imaginations in 1961. With it will come better methods for ES and lineage-specific stem cell identification, maintenance, and controlled fate selection. Clearly, ES cell models are already providing opportunities for the est- lishment of limitless sources of specific cell populations. In recognition of the gr- ing excitement and potential of ES cells as models for both the advancement of basic science and future clinical applications, I felt it timely to edit this collection of pro- cols (Embryonic Stem Cells) in which forefront investigators would provide detailed methods for use of ES cells to study various lineages and tissue types.
Book Synopsis Human Molecular Biology Laboratory Manual by : Stefan Surzycki
Download or read book Human Molecular Biology Laboratory Manual written by Stefan Surzycki and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Book Synopsis Nonisotopic Probing, Blotting, and Sequencing by : Larry J. Kricka
Download or read book Nonisotopic Probing, Blotting, and Sequencing written by Larry J. Kricka and published by Elsevier. This book was released on 1995-06-01 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. - Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules - Gives protocols for nonisotopic DNA sequencing - new in this edition - Gives extensive, practical information - Presents background information for each method - Provides expert accounts from the inventor or developer of each method - Contains seven entirely new chapters - Covers all major types of nonisotopic procedures for labeling and detection
Book Synopsis DNA Fingerprinting in Plants and Fungi by : Kurt Weising
Download or read book DNA Fingerprinting in Plants and Fungi written by Kurt Weising and published by CRC Press. This book was released on 1994-11-22 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This exciting new book describes the use of DNA fingerprinting and its application in a wide area of plant and fungal research. It presents a thorough theoretical introduction to DNA fingerprinting, the practical aspects of extraction of DNA from the plant or fungus under study, and the statistical analysis of the data. An overview presents all species to which DNA fingerprinting is currently being applied and highlights many future technical developments.
Book Synopsis Molecular Embryology by : Paul T. Sharpe
Download or read book Molecular Embryology written by Paul T. Sharpe and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 752 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most people have some interest in embryos; this probably results, in part, from their interest in understanding the biological origins of themselves and their offspring and, increasingly, concerns about how environmental change such as pollution might affect human development. Obviously, et- cal considerations preclude experimental studies of human embryos and, c- sequently, the developmental biologist has turned to other species to examine this process. Fortunately, the most significant conclusion to be drawn from the experimental embryology of the last two decades is the manner in which orthologous or closely related molecules are deployed to mediate similar - velopmental processes in both vertebrates and invertebrates. The molecular mechanisms regulating processes fundamental to most animals, such as axial patterning or axon guidance, are frequently conserved during evolution. (It is now widely believed that the differences between phyla and classes are the result of new genes, arising mostly by duplication and divergence of extant sequences, regulating the appearance of derived characters. ) Other vertebrates are obviously most likely to use the same devel- mental mechanisms as humans and, within the vertebrate subphylum, the - parent degree of conservation of developmental mechanism is considerable. It has long been recognized that particular vertebrate species offer either d- tinct advantages in investigating particular stages of development or are - pecially amenable to particular manipulations. No single animal can provide all the answers because not all types of experiments can be carried out on a single species.
Download or read book Antenna Handbook written by Y.T. Lo and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 2282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques based on the method of modal expansions, the Rayleigh-Stevenson expansion in inverse powers of the wavelength, and also the method of moments solution of integral equations are essentially restricted to the analysis of electromagnetic radiating structures which are small in terms of the wavelength. It therefore becomes necessary to employ approximations based on "high-frequency techniques" for performing an efficient analysis of electromagnetic radiating systems that are large in terms of the wavelength. One of the most versatile and useful high-frequency techniques is the geometrical theory of diffraction (GTD), which was developed around 1951 by J. B. Keller [1,2,3]. A class of diffracted rays are introduced systematically in the GTD via a generalization of the concepts of classical geometrical optics (GO). According to the GTD these diffracted rays exist in addition to the usual incident, reflected, and transmitted rays of GO. The diffracted rays in the GTD originate from certain "localized" regions on the surface of a radiating structure, such as at discontinuities in the geometrical and electrical properties of a surface, and at points of grazing incidence on a smooth convex surface as illustrated in Fig. 1. In particular, the diffracted rays can enter into the GO shadow as well as the lit regions. Consequently, the diffracted rays entirely account for the fields in the shadow region where the GO rays cannot exist.
Book Synopsis Bone Research Protocols by : Miep H. Helfrich
Download or read book Bone Research Protocols written by Miep H. Helfrich and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).
Book Synopsis DNA polymorphisms in eukaryotic genomes by : Arbeitsgemeinschaft für Gen-Diagnostik. Annual Meeting
Download or read book DNA polymorphisms in eukaryotic genomes written by Arbeitsgemeinschaft für Gen-Diagnostik. Annual Meeting and published by . This book was released on 1992 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Symbiotic Nitrogen Fixation by : P. Graham
Download or read book Symbiotic Nitrogen Fixation written by P. Graham and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past three decades there has been a large amount of research on biological nitrogen fixation, in part stimulated by increasing world prices of nitrogen-containing fertilizers and environmental concerns. In the last several years, research on plant--microbe interactions, and symbiotic and asymbiotic nitrogen fixation has become truly interdisciplinary in nature, stimulated to some degree by the use of modern genetic techniques. These methodologies have allowed us to make detailed analyses of plant and bacterial genes involved in symbiotic processes and to follow the growth and persistence of the root-nodule bacteria and free-living nitrogen-fixing bacteria in soils. Through the efforts of a large number of researchers we now have a better understanding of the ecology of rhizobia, environmental parameters affecting the infection and nodulation process, the nature of specificity, the biochemistry of host plants and microsymbionts, and chemical signalling between symbiotic partners. This volume gives a summary of current research efforts and knowledge in the field of biological nitrogen fixation. Since the research field is diverse in nature, this book presents a collection of papers in the major research area of physiology and metabolism, genetics, evolution, taxonomy, ecology, and international programs.
Book Synopsis Molecular Methods in Plant Pathology by : U. S. Singh
Download or read book Molecular Methods in Plant Pathology written by U. S. Singh and published by CRC Press. This book was released on 2017-12-14 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.
