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Characterization Of Post Translational Modification And Protein Protein Interaction Of Rna Binding Protein 1 Rbp1p In Saccharomyces Cerevisiae
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Book Synopsis Characterization of Post-translational Modification and Protein-protein Interaction of RNA-binding Protein 1, Rbp1p, in Saccharomyces Cerevisiae by : 方瓊瑩
Download or read book Characterization of Post-translational Modification and Protein-protein Interaction of RNA-binding Protein 1, Rbp1p, in Saccharomyces Cerevisiae written by 方瓊瑩 and published by . This book was released on 2007 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Characterization of Posttranslational Modification of RNA Binding Protein, Rbp1p, in Saccharomyces Cerevisiae Under Stress Conditions by : 蔡松蒲
Download or read book Characterization of Posttranslational Modification of RNA Binding Protein, Rbp1p, in Saccharomyces Cerevisiae Under Stress Conditions written by 蔡松蒲 and published by . This book was released on 2009 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Functional Characterization of RNA-binding Protein Rbp1p and Its Interacting Proteins in Saccharomyces Cerevisiae by : 張翎君
Download or read book Functional Characterization of RNA-binding Protein Rbp1p and Its Interacting Proteins in Saccharomyces Cerevisiae written by 張翎君 and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Role of Protein Post-Translational Modifications in Protein-RNA Interactions and RNP Assemblies by : Roberto Giambruno
Download or read book The Role of Protein Post-Translational Modifications in Protein-RNA Interactions and RNP Assemblies written by Roberto Giambruno and published by Frontiers Media SA. This book was released on 2022-02-11 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Nicolas Lux Fawzi is a member of the Scientific Advisory Board of Dewpoint Therapeutics LLC. All other Topic Editors declare no competing interests with regards to the Research Topic.
Book Synopsis Characterization of Novel RNA-protein Regulatory Interactions in Saccharomyces Cerevisiae by : Nikoleta Georgieva Tsvetanova
Download or read book Characterization of Novel RNA-protein Regulatory Interactions in Saccharomyces Cerevisiae written by Nikoleta Georgieva Tsvetanova and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The dynamic processes of a living cell depend on the coordinated temporal and spatial regulation of the many steps of gene expression. Transcription regulation is one control point of gene expression, and a gene can also be regulated post-transcriptionally, by RNA-binding proteins (RBPs). The biological significance of post-transcriptional regulation is especially evident in cases, where RBP binding controls the temporal precision of suppression and activation of important cellular stress responses. We developed a proteome-wide experimental approach for in vitro identification of novel RBPs and RNA-protein interactions in Saccharomyces cerevisiae. We found 12 novel RNA-binding proteins, the majority of which, surprisingly, are currently annotated as enzymes with roles in metabolic processes. We next used this proteomic approach to screen for proteins specifically interacting with the HAC1 RNA, which mediates activation of the yeast unfolded protein response (UPR). We found that HAC1 associated reproducibly with four small yeast GTPases, three of which are of the Ypt family of ras-GTPases. We further characterized one of them, the yeast Rab1 homolog Ypt1, and showed that Ypt1 interacted with unspliced HAC1 RNA only in the absence of ER stress. Selective Ypt1 depletion increased HAC1 RNA stability and expression, and also affected timely recovery from UPR. By developing and applying a novel proteomic approach for studying RNA-protein interactions, we established Ypt1 as an important regulator of HAC1 expression and UPR signaling. This unexpected protein-RNA interaction provides a biochemical mechanism for coordinating the key cellular processes of vesicle trafficking and ER homeostasis.
Book Synopsis Mapping RNA Protein Interactions in Saccharomyces Cerevisiae by : Daniel Michael Klass
Download or read book Mapping RNA Protein Interactions in Saccharomyces Cerevisiae written by Daniel Michael Klass and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: We are on the threshold of a new era in our understanding of that fantastic feat of regulation at the core of life itself--gene expression. The rapid pace of new developments in genome-wide, high-throughput technologies has allowed us unprecedented access to observe multiple stages of the gene expression program for nearly the entire genome. This has revealed a widespread discordance between mRNA abundance and protein abundance for many genes whose expression changes in response to environmental stimuli, and a significant coordination of post-transcriptional regulation for specific sets of related mRNAs at the levels localization, translation, decay, and the noise in gene expression. Despite this evidence suggesting the existence of a coordinated regulatory framework that potentially affects the fate of every mRNA in the cell, our efforts to discern the underlying structure and regulatory themes are hindered by an incomplete understanding of RNA-protein interactions. To advance our comprehension of post-transcriptional regulation, we developed new tools to identify which proteins bind to RNA, which of those bind concurrently, which RNAs are bound by a given protein, and where each protein binds on each RNA. Using our proteomic tools we discovered hundreds unexpected RNA binding proteins, uncovered new RNA binding domains, identified widespread, concurrent binding with several RNA binding proteins, and inferred functional information from the simultaneous binding partners of several RNA binding proteins. We used our genomic, sequencing-based tools to systematically interrogate a large set of diverse RNA binding proteins and we discerned new themes from the resulting data. This revealed significant differences in function, localization, and regulation among the proteins encoded by the targets of a given RNA binding protein based on binding position. These results suggest that the functional consequences of the RBP-RNA interaction are determined not only by whether an mRNA is bound by an RBP but also by the position of the binding site within the mRNA and its relation to the other RBPs that bind the same mRNA. Overall, we found evidence of an extensive regulatory framework involving hundreds of RNA binding proteins, encompassing nearly the entire transcriptome, and extending our understanding of the RNA-protein interactions at the heart of post-transcriptional regulation.
Book Synopsis Role of Post-transcriptional Modifications of Transfer RNA and Post-translational Modification of Ribosomal Protein in Regulating Translational Accuracy in Saccharomyces Cerevisiae by : Kartikeya Joshi
Download or read book Role of Post-transcriptional Modifications of Transfer RNA and Post-translational Modification of Ribosomal Protein in Regulating Translational Accuracy in Saccharomyces Cerevisiae written by Kartikeya Joshi and published by . This book was released on 2017 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: Misreading errors are a frequent source of error during translation that result in incorporation of a wrong amino acid in the growing polypeptide chain and can potentially generate a misfolded protein. Studies have estimated misreading errors to occur with a frequency of 10−3 - 10−4 errors per codon. However, recent studies in E. coli have determined that the frequency of misreading errors is in fact 100-fold lower (10−6 errors per codon) for most near-cognate codons. I developed a novel reporter based assay that can be used to quantify errors by a single tRNA(Glu UUC) in Saccharomyces cerevisiae. Using this reporter I find that the rate of misreading errors in yeast can vary from 10-6 - 10-4 errors per codon. I also find that the rate of errors in yeast is about 3-fold lower than that in bacteria. Another important conclusion made from this study is the occurrences of errors at the wobble position in yeast. Using the Beta-galactosidase reporter system developed in this study and the dual luciferase reporter system previously developed in our lab, I characterized the function of post-transcriptional modifications present in the anticodon stem loop of tRNA(Glu UUC) and tRNA(Lys UUU). I find that both mcm5s2U34 and t6A3-- modification regulate decoding accuracy during translation in a codon context dependent manner. mcm5s2U34 was found to promote errors for A- ending codons, while restricting errors for G- ending codons. Similarly, t6A3-- is found to restrict U·U mismatch at the first codon anticodon position but promotes the same error at the wobble position. Using genetic studies and our reporter system I established that Ctk1-dependent phosphorylation at serine 176 on ribosomal protein uS5 is critical in maintaining accuracy during translation. I show that loss of this phosphorylation site can increase misreading errors, while the presence of a phosphomimetic mutation at position 176 on uS5 can make a yeast strain hyper accurate. This study elucidates the important role played by modifications of the translational apparatus during decoding.
Book Synopsis Candida Albicans by : Rajendra Prasad
Download or read book Candida Albicans written by Rajendra Prasad and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: Candida, which was discovered more than a century ago as a causative organism of oral thrush, is now thought to potentially infect almost every tissue of the human body. Although we still do not have a safe anti-candida drug, the growing pace of progess of research on Candida albicans holds promise that a breakthrough is imminent. Though many monographs and articles on candida and candidoses have appeared in recent years, they mostly cover the clinical aspects. This particular text, however, explains the more basic features of candida including the molecular genetics, molecular biology and immunology of the cell wall, the molecular basis of morphogenesis and the structure and function of the plasma membrane. The role of anti-candida drugs and their mechanism of action are also discussed.
Book Synopsis Eukaryotic MRNA Processing by : Adrian Krainer
Download or read book Eukaryotic MRNA Processing written by Adrian Krainer and published by IRL Press. This book was released on 1997 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example duringembryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and catalytically active spliceosomes; the structure and mechanism of action of small nuclear ribonucleoprotein particles and proteinfactors that catalyse pre-mRNA splicing in mammalian cells and in yeast; the regulation of gene expression and generation of protein isoform diversity by alternative splicing; the mechanisms of 3' end cleavage and polyadenylation; the architecture of the cell nucleus in relation to these processesand to the localization of the relevant substrates and factors; the diverse mechanisms of RNA processing by ribozymes and their potential relevance for nuclear mRNA processing; the mechanism of spliced-leader addition by trans-splicing in nematodes and trypanosomes; and the process ofinsertion/deletion mRNA editing in kinetoplasmid protozoa. In each chapter, leading researchers have provided detailed, critical reviews of the history, experimental approaches, major advances, current ideas and models, as well as future directions, for each of these active areas of research.
Book Synopsis The Eukaryotic RNA Exosome by : John LaCava
Download or read book The Eukaryotic RNA Exosome written by John LaCava and published by Springer Nature. This book was released on 2019-11-25 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes’ origins in prokaryotes, probe its RNA substrates, adapter complexes and macromolecular interaction of networks, and establish critical structural-function relationships. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field.
