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Characterization Of Dendrimers With Reversed Phase High Performance Liquid Chromatography Capillary Electrophoresis Mass Spectrometry And Polyacrylamide Gel Electrophoresis
Download Characterization Of Dendrimers With Reversed Phase High Performance Liquid Chromatography Capillary Electrophoresis Mass Spectrometry And Polyacrylamide Gel Electrophoresis full books in PDF, epub, and Kindle. Read online Characterization Of Dendrimers With Reversed Phase High Performance Liquid Chromatography Capillary Electrophoresis Mass Spectrometry And Polyacrylamide Gel Electrophoresis ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Characterization of Dendrimers with Reversed Phase High Performance Liquid Chromatography, Capillary Electrophoresis, Mass Spectrometry and Polyacrylamide Gel Electrophoresis by : ShaRhonda Dennis
Download or read book Characterization of Dendrimers with Reversed Phase High Performance Liquid Chromatography, Capillary Electrophoresis, Mass Spectrometry and Polyacrylamide Gel Electrophoresis written by ShaRhonda Dennis and published by . This book was released on 2007 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Spectroscopic Characterization of Polyamido Amine Dendrimers and Their Application in High Performance Liquid Chromatography by : Charlotte Lynn Larson
Download or read book Spectroscopic Characterization of Polyamido Amine Dendrimers and Their Application in High Performance Liquid Chromatography written by Charlotte Lynn Larson and published by . This book was released on 2001 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Index Medicus written by and published by . This book was released on 2003 with total page 1696 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Book Synopsis Synthesis and Characterization of Covalently-linked Dendrimer Bioconjugates and the Non-covalent Self-assembly of Streptavidin-based Megamers by : Megan E. McLean
Download or read book Synthesis and Characterization of Covalently-linked Dendrimer Bioconjugates and the Non-covalent Self-assembly of Streptavidin-based Megamers written by Megan E. McLean and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This work details the attachment of dendrimers to proteins, peptides and single stranded DNA (ssDNA). Dendrimers based on melamine satisfy many of the synthetic demands in the field of bioconjugate chemistry including: monodispersity, synthetic flexibility and scalability. The solution-phase syntheses of both ssDNA-dendrimer and peptide-dendrimer bioconjugates is described, and thorough characterization by matrix-assisted laser desorption ionization/ time-of-flight (MALDI-TOF) mass spectrometry, UV-vis spectroscopy, fluorescence spectroscopy, and polyacrylamide gel electrophoresis is discussed. Non-covalent DNA-dendrimer complexes have been shown to facilitate antisense gene delivery, but are vulnerable to dissociation and subsequent enzymatic degradation within the cell. In an effort to prepare biocompatible antisense agents capable of effectively shielding ssDNA from intracellular nuclease digestion, disulfide-linked ssDNA-dendrimers were prepared and rigorously characterized to rule out the possibility of an electrostatic-based interaction. Hybridization assays were performed to determine if the covalently-attached dendrimer affected the ability of the attached ssDNA strand to anneal with a complementary sequence to form double-stranded DNA (dsDNA)-dendrimers. Results indicate that ssDNA-dendrimer conjugates readily anneal to complementary ssDNA strands either in solution or attached to gold surfaces. Nuclease digestions of conjugates in solution suggested that enzymatic manipulation of dsDNA-dendrimers is possible, offering promise for DNA-based computation and other fields of DNA-nanotechnology. Much larger bioconjugates consisting of dendrimers, proteins and peptides were prepared with the goal of obtaining molecular weights sufficient for enhanced permeability and retention (EPR) in tumors. While the dendrimer provides the advantages of a purely synthetic route for drug delivery, the protein portion of the bioconjugate provides a monodisperse, macromolecular scaffold for the non-covalent self-assembly of the dendrimers. The strategy presented herein is based on the strong interaction between biotin and the 60 kD tetrameric protein streptavidin. Each monomer of streptavidin is capable of binding 1 biotin molecule, thus when biotin functionalized peptide-dendrimers are added to streptavidin they bind to form a cluster of dendrimers, or a megamer. The biotinylated peptides that link the dendrimers to the streptavidin core provide a way to actively target specific cell types for drug delivery. Megamer formation through the addition of tetrameric streptavidin was successful as indicated by MALDI-TOF, UV-vis titration and gel electrophoresis assays.
Book Synopsis Synthesis and Characterization of Melamine-based Dendrimers with Potential Biological Applications by : Hannah Louise Crampton
Download or read book Synthesis and Characterization of Melamine-based Dendrimers with Potential Biological Applications written by Hannah Louise Crampton and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The convergent strategy towards dendrimer synthesis is well-suited to generate macromolecules with a diverse periphery, at the expense of time and effort, while the divergent strategy has historically been effective at yielding higher generation dendrimers, although they are often plagued by impurities. Both the convergent and divergent routes were applied to the synthesis of melamine-based dendrimers, offering a comparison of the routes within a system. Generation-1 dendrons heterogeneously functionalized with Boc-protected amines and hydrazones were synthesized convergently and coupled to a generation-1 tris(piperazine) core to yield a generation-2 dendrimer bearing 18 Boc-amines and three hydrazones. Although the yield for the final coupling step was rather low (56%), the yields for all intermediate steps were quite high. Attempts toward obtaining a generation-3 dendrimer through this route were unsuccessful due presumably to steric hindrance. The materials obtained showed no impurities in their 1H and 13C NMR and mass spectra, although several chromatographic purifications were necessary throughout the synthesis. A divergent strategy based on addition of a dichlorotriazine monomer to polyamine cores was used to synthesize dendrimers of generations 1-5. All intermediates and dendrimers were either purified by precipitation, or did not need purification. 1H NMR spectroscopy indicated that reactions were complete up to G4-NH2 by integration, and mass spectroscopy confirmed that assignment. HPLC and GPC of Gn-Cl dendrimers showed sharp peaks for G1-G3, but G4-Cl appeared to have a small amount of impurities that are similar in size and polarity to the fully-substituted dendrimer. The G1-G3 dendrimers were confidently assigned as pure by conventional organic chemistry standards, but the assignment of purity to higher generations remained tentative. A G1-Cl dendrimer was functionalized with imidazole, and then deprotected and PEGylated with PEG5000 to yield a water soluble dendrimer. The imidazole-capped, Boc-protected dendrimer and the deprotected dendrimer were characterized by 1H and 13C NMR spectroscopy and mass spectrometry. The degree of PEGylation on the PEGylated material could not be definitively ascertained; however, the material is capable of solubilizing very hydrophobic Zn-phthalocyanines in water.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1998 with total page 1872 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Telomerase Inhibition by : Lucy Andrews
Download or read book Telomerase Inhibition written by Lucy Andrews and published by Springer Science & Business Media. This book was released on 2007-11-29 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition. The techniques described provide the researcher with a diverse and comprehensive set of tools for the study of telomerase inhibition. The volume is aimed at biochemists, molecular biologists, cancer researchers, and geneticists.
Book Synopsis Peptide-Lipid Interactions by : Sidney A. Simon
Download or read book Peptide-Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction
Author :Radislav A. Potyrailo Publisher :Springer Science & Business Media ISBN 13 :0387737138 Total Pages :495 pages Book Rating :4.3/5 (877 download)
Book Synopsis Combinatorial Methods for Chemical and Biological Sensors by : Radislav A. Potyrailo
Download or read book Combinatorial Methods for Chemical and Biological Sensors written by Radislav A. Potyrailo and published by Springer Science & Business Media. This book was released on 2009-03-21 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical sensors are in high demand for applications as varied as water pollution detection, medical diagnostics, and battlefield air analysis. Designing the next generation of sensors requires an interdisciplinary approach. The book provides a critical analysis of new opportunities in sensor materials research that have been opened up with the use of combinatorial and high-throughput technologies, with emphasis on experimental techniques. For a view of component selection with a more computational perspective, readers may refer to the complementary volume of Integrated Analytical Systems edited by M. Ryan et al., entitled “Computational Methods for Sensor Material Selection”.
Book Synopsis Bioinformatics for Glycobiology and Glycomics by : Claus-Wilhelm von der Lieth
Download or read book Bioinformatics for Glycobiology and Glycomics written by Claus-Wilhelm von der Lieth and published by John Wiley & Sons. This book was released on 2009-10-01 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first to be dedicated to the bioinformatics of carbohydrates and glycoproteins. It provides an introduction to this emerging field of science both for the experimentalist working in glycobiology and glycomics, and also for the computer scientist looking for background information for the development of highly sophisticated algorithmic approaches. The book provides an overview of the state-of-the-art in the field, with reviews on databases, and the tools in use for analysis, interpretation, and prediction of the structures of complex carbohydrates, and demonstrates the value of bioinformatics for glycobiology. The availability of comprehensive databases and corresponding bioinformatics tools, to access and analyse the large amounts of experimental data relating to the structure of carbohydrates, will be a prerequisite for the success of the large-scale glycomics projects that aim to decipher new, so far unknown, biological functions of glycans. Efficient bioinformatics descriptions and tools can considerably enhance the efficiency of glycomics research, in terms of data quality, analysis and experimental costs. For a complete understanding of the molecular processes in which carbohydrates are involved, such as protein–carbohydrate interactions and the impact of glycosylation on protein function, knowledge of the 3D structure of the carbohydrate, the protein–carbohydrate complex, or the glycoprotein is often indispensable. This book provides a thorough introduction into methods used for conformational analysis of carbohydrates. Key features: Describes bioinformatic approaches to handle carbohydrate-active enzymes and glycosylation. Provides an overview on bioinformatics tools that facilitate analysis of carbohydrate structures. Gives introduction into molecular modelling of carbohydrate 3D structure and carbohydrates contained in the Protein Databank. Assumes only a basic knowledge of biology and bioinformatics.
Book Synopsis Molecular Diagnostics: Promises and Possibilities by : Mousumi Debnath
Download or read book Molecular Diagnostics: Promises and Possibilities written by Mousumi Debnath and published by Springer Science & Business Media. This book was released on 2010-01-29 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.
Book Synopsis Mass Spectrometry by : Jürgen H Gross
Download or read book Mass Spectrometry written by Jürgen H Gross and published by Springer Science & Business Media. This book was released on 2006-04-05 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry is an ideal textbook for students and professionals as well as newcomers to the field. Starting from the very first principles of gas-phase ion chemistry and isotopic properties, the textbook takes the reader through the design of mass analyzers and ionization methods all the way to mass spectral interpretation and coupling techniques. Step-by-step, the reader learns how mass spectrometry works and what it can do. The book comprises a balanced mixture of practice-oriented information and theoretical background. It features a clear layout and a wealth of high-quality figures. Exercises and solutions are located on the Springer Global Web.
Download or read book HPLC Columns written by Uwe D. Neue and published by Wiley-VCH. This book was released on 1997-07-28 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include: * Comprehensive overview of state-of-the-art HPLC column technology * Explanation of the underlying principles of HPLC columns * Methods for selecting columns * Practical advice on using and applying columns, including examples * Section by M. Zoubair El Fallah on methods development * Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed * Troubleshooting section HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses.
Book Synopsis Dendrimer Chemistry by : Fritz Vögtle
Download or read book Dendrimer Chemistry written by Fritz Vögtle and published by John Wiley & Sons. This book was released on 2009-03-09 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by internationally acclaimed authors, this textbook contains everything you need to know about this versatile class of compounds. Starting with a historical overview, definitions and other fundamentals, it goes on to look at characterization, analysis and properties of dendrimers. While the focus is on synthesis and applications, it also contains chapters on analytics and other applications. Essential reading for organic and polymer chemists, undergraduate and graduate students, students and lecturers in chemistry.
Book Synopsis Engineering Polymer Systems for Improved Drug Delivery by : Rebecca A. Bader
Download or read book Engineering Polymer Systems for Improved Drug Delivery written by Rebecca A. Bader and published by John Wiley & Sons. This book was released on 2014-01-17 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polymers have played a critical role in the rational design and application of drug delivery systems that increase the efficacy and reduce the toxicity of new and conventional therapeutics. Beginning with an introduction to the fundamentals of drug delivery, Engineering Polymer Systems for Improved Drug Delivery explores traditional drug delivery techniques as well as emerging advanced drug delivery techniques. By reviewing many types of polymeric drug delivery systems, and including key points, worked examples and homework problems, this book will serve as a guide to for specialists and non-specialists as well as a graduate level text for drug delivery courses.
Book Synopsis Electrochemical Immunosensors and Aptasensors by : Paolo Ugo
Download or read book Electrochemical Immunosensors and Aptasensors written by Paolo Ugo and published by MDPI. This book was released on 2018-03-23 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Electrochemical Immunosensors and Aptasensors" that was published in Chemosensors
Book Synopsis Evaluation Technologies for Food Quality by : Jian Zhong
Download or read book Evaluation Technologies for Food Quality written by Jian Zhong and published by Woodhead Publishing. This book was released on 2019-04-16 with total page 914 pages. Available in PDF, EPUB and Kindle. Book excerpt: Evaluation Technologies for Food Quality summarizes food quality evaluation technologies, which include sensory evaluation techniques and chemical and physical analysis. In particular, the book introduces many novel micro and nano evaluation techniques, such as atomic force microscopy, scanning electron microscopy, and other nanomaterial-based methods. All topics cover basic principles, procedures, advantages, limitations, recent technology development, and application progress in different types of foods. This book is a valuable resource for scientists in the field of food science, engineering, and professionals in the food industry, as well as for undergraduate and postgraduate students studying food quality evaluation technology. Explains basic principles, procedures, advantages, limitations, and current applications of recent food quality technologies Provides guidance on the understanding and application of food quality evaluation technology in the field of food research and food industry Introduces many novel micro/nano evaluation techniques, such as atomic force and scanning electron microscopies and other nanomaterial-based methods
