Author : Jon-Michael Reed
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (658 download)
Book Synopsis Characterization and Profiling of the SUMO Conjugation Pathway in Populus by : Jon-Michael Reed
Download or read book Characterization and Profiling of the SUMO Conjugation Pathway in Populus written by Jon-Michael Reed and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Covalent attachment of the Small Ubiquitin-like Modifier (SUMO) to proteins in eukaryotic cells has been demonstrated to be an important step in the regulation of a wide variety of cellular processes. Such processes include transcriptional regulation, mediating DNA/protein interaction, and increasing protein stability by interfering with the ubiquitin conjugation pathway in some cases. While much work has been done to characterize the SUMO pathway in yeast, insect, and mammalian systems, relatively little is known about the roles that SUMO conjugation plays in plants. In this study, I make use of the Populus model system to investigate the gene structures of the various family members of the SUMO pathway in plants, as well as demonstrate that the pathway is active in a number of tissue types. Additionally, we show that the SUMOylation is inducible through acute stress treatments such as desiccation, heat shock, and peroxide application. To further our understanding of the SUMOylation process, I have set out to identify SUMO-conjugated proteins from heat shock treated tissues through a multi-step purification process.