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Centrifugation Techniques
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Download or read book Centrifugation written by David Rickwood and published by . This book was released on 1984 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edition provides extensive experimental details of protocols for all types of centrifugal separations from macrmolecules to whole cells. It also describes the applications of centrifuges ranging from simle bench machines to analytical centrifuges.
Book Synopsis Centrifugation Techniques by : Shalinee Naidoo
Download or read book Centrifugation Techniques written by Shalinee Naidoo and published by Arcler Press. This book was released on 2017-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell biology involves the analysis of cells and tissues at both the morphological and biochemical levels in an attempt to understand the molecular mechanisms operating in any living organism. Proper biochemical analysis of biological samples is imperative for constructing complete and true cell biology. The subcellular organization of cells can be studied via electron microscopy or by the separation and subsequent molecular and biochemical analysis of individual cellular components. While electron microscopy provides detailed information about the appearance of cellular organelles, not much information can be obtained regarding their individual functions. As such, a way to separate the different organelles from remaining cellular components was needed (OpenLearn, 2017). This was made possible by a technique known as cell fractionation which allows for the isolation of various cellular components (nuclear, cytosolic and mitochondrial) based on their subcellular compartmentalized distribution followed by analysis of their molecular information ranging from the state and distribution of a cell or subcellular component to their response to a range of treatments (Dimauro et al, 2012). The separation of cellular components and particles by sedimentation is one of the most powerful tools in biology. Even though sedimentation using centrifugation is not a new technology, it is essential for conducting cutting edge genomic and proteomic research, as it aids in providing purified particles of interest. As such, centrifugation forms one of the most important and widely applied research techniques used in various disciplines today. It services a range of research fields such as biochemistry, cellular and molecular biology while allowing large scale applications across a broad spectrum of industries ranging from large scale commercial applications in the pharmaceutical and agricultural industries to small scale laboratory-scale scientific studies. Miescher was one of the first researchers to use a centrifuge for the isolation of a cell organelle when he separated nuclei from human pus cells in 1871. A survey conducted at the US National Institutes of Health revealed that over 65% of research workers believe that using centrifugation to purify cells, subcellular organelles, viruses, proteins, and nucleic acids is an essential part of their everyday research, further proving the importance of this technique in today's scientific world (Sigma-Aldrich, 2011). Current research applications rely on centrifugation for the isolation and harvest of cells, sub cellular components and macromolecules in high yields. It is commonly used as a separation technique when gravity separation is too slow and particles within the sample to be separated do not settle readily or at all. This technique has since paved the way for the determination and understanding of fundamental cellular processes such as oxidative phosphorylation, intracellular digestion, protein synthesis as well as transportation of substances in and out of the cell (Graham and Rickwood). Thus centrifuges are now regarded as one of the most basic yet valuable pieces of equipment required in biological laboratories. This book aims to provide an introduction into the history and principles of centrifugation as well as an in depth explanation of the history and principles surrounding centrifugation. It also aims to provide an explanation of commonly used centrifugation techniques and their related practical applications in various industries. Furthermore, to aid in understanding the various principles behind this technique, the basic design of various rotors have been diagrammatically presented. Also in this book is an overview of the currently available centrifuge types and related instrument components as well as a description of centrifugal theory, a table comparing densities of commonly separated macromolecules and additional information on separation techniques together with a comprehensive advice for proper centrifuge handling, maintenance and related safety protocols. There is also further information on sample preparation and and how to design an ideal centrifugation protocol.
Book Synopsis Molecular Biology of The Cell by : Bruce Alberts
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Principles and Techniques of Biochemistry and Molecular Biology by : Keith Wilson
Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Book Synopsis An Introduction to Centrifugation by : T. C. Ford
Download or read book An Introduction to Centrifugation written by T. C. Ford and published by Taylor & Francis. This book was released on 1991 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: This introduction explains what centrifugation is, what it does, how it does it, and the types of centrifuges, rotors, techniques, and density-gradient media currently available.
Book Synopsis Centrifugation techniques by : Shalinee Naidoo
Download or read book Centrifugation techniques written by Shalinee Naidoo and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Industrial Centrifugation Technology by : Wallace Woon-Fong Leung
Download or read book Industrial Centrifugation Technology written by Wallace Woon-Fong Leung and published by McGraw Hill Professional. This book was released on 1998 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction General Principles of Sedimenting Centrifuges Batch Sedimenting Centrifuges Continous Sedimenting Centrifuges Applications of Sedimenting Centrifuges Continous-Feed Sedimentation General Principles of Filtering Centrifuges Batch Filtering Centrifuges Continuous Filtering Centrifuges Applications of Filtering Centrifuges Feed Acceleration Lab, Pilot, and Production Tests Centrifuge Selection and Sizing Optimization and Troubleshooting Kaolin Processing Dewatering of Compactible Solids Cake compaction theory Appendices Name Index Subject Index.
Book Synopsis Biological Centrifugation by : Dr John Graham
Download or read book Biological Centrifugation written by Dr John Graham and published by Garland Science. This book was released on 2020-12-17 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques.
Book Synopsis Centrifugal Separations in Molecular and Cell Biology by : G.D. Birnie
Download or read book Centrifugal Separations in Molecular and Cell Biology written by G.D. Birnie and published by Butterworth-Heinemann. This book was released on 2014-06-28 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology.
Book Synopsis Industrial Centrifugation Technology by : Wallace Woon-Fong Leung
Download or read book Industrial Centrifugation Technology written by Wallace Woon-Fong Leung and published by McGraw Hill Professional. This book was released on 1998 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction General Principles of Sedimenting Centrifuges Batch Sedimenting Centrifuges Continous Sedimenting Centrifuges Applications of Sedimenting Centrifuges Continous-Feed Sedimentation General Principles of Filtering Centrifuges Batch Filtering Centrifuges Continuous Filtering Centrifuges Applications of Filtering Centrifuges Feed Acceleration Lab, Pilot, and Production Tests Centrifuge Selection and Sizing Optimization and Troubleshooting Kaolin Processing Dewatering of Compactible Solids Cake compaction theory Appendices Name Index Subject Index.
Book Synopsis Modern Sample Preparation for Chromatography by : Serban C. Moldoveanu
Download or read book Modern Sample Preparation for Chromatography written by Serban C. Moldoveanu and published by Elsevier. This book was released on 2021-02-24 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern Sample Preparation for Chromatography, Second Edition explains the principles of sample preparation for chromatographic analysis. A variety of procedures are applied to make real-world samples amenable for chromatographic analysis and to improve results. This book's authors discuss each procedure’s advantages, disadvantages and their applicability to different types of samples, along with their fit for different types of chromatographic analysis. The book contains numerous literature references and examples of sample preparation for different matrices and new sections on green approaches in sample preparation, progress in automation of sample preparation, non-conventional solvents for LLE (ionic liquids, deep eutectic mixtures, and others), and more. Presents numerous techniques applied for sample preparation for chromatographic analysis Provides an up-to-date source of information regarding the progress made in sample preparation for chromatography Describes examples for specific types of matrices, providing a guide for choosing the appropriate sample preparation method for a given analysis
Book Synopsis Density Gradient Centrifugation by :
Download or read book Density Gradient Centrifugation written by and published by Elsevier. This book was released on 1978-01-01 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: Density Gradient Centrifugation
Book Synopsis Comprehensive Sampling and Sample Preparation by : Josep M. Bayona
Download or read book Comprehensive Sampling and Sample Preparation written by Josep M. Bayona and published by Academic Press. This book was released on 2012-12-31 with total page 2998 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive Sampling and Sample Preparation is a complete treatment of the theory and methodology of sampling in all physical phases and the theory of sample preparation for all major extraction techniques. It is the perfect starting point for researchers and students to design and implement their experiments and support those experiments with quality-reviewed background information. In its four volumes, fundamentals of sampling and sample preparation are reinforced through broad and detailed sections dealing with Biological and Medical, Environmental and Forensic, and Food and Beverage applications. The contributions are organized to reflect the way in which analytical chemists approach a problem. It is intended for a broad audience of analytical chemists, both educators and practitioners of the art and can assist in the preparation of courses as well in the selection of sampling and sample preparation techniques to address the challenges at hand. Above all, it is designed to be helpful in learning more about these topics, as well as to encourage an interest in sampling and sample preparation by outlining the present practice of the technology and by indicating research opportunities. Sampling and Sample preparation is a large and well-defined field in Analytical Chemistry, relevant for many application areas such as medicine, environmental science, biochemistry, pharmacology, geology, and food science. This work covers all these aspects and will be extremely useful to researchers and students, who can use it as a starting point to design and implement their experiments and for quality-reviewed background information There are limited resources that Educators can use to effectively teach the fundamental aspects of modern sample preparation technology. Comprehensive Sampling and Sample Preparation addresses this need, but focuses on the common principles of new developments in extraction technologies rather than the differences between techniques thus facilitating a more thorough understanding Provides a complete overview of the field. Not only will help to save time, it will also help to make correct assessments and avoid costly mistakes in sampling in the process Sample and sample preparation are integral parts of the analytical process but are often less considered and sometimes even completely disregarded in the available literature. To fill this gap, leading scientists have contributed 130 chapters, organized in 4 volumes, covering all modern aspects of sampling and liquid, solid phase and membrane extractions, as well as the challenges associated with different types of matrices in relevant application areas
Book Synopsis Analytical Ultracentrifugation in Biochemistry and Polymer Science by : Stephen E. Harding
Download or read book Analytical Ultracentrifugation in Biochemistry and Polymer Science written by Stephen E. Harding and published by . This book was released on 1992 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book of its kind to appear for nearly two decades and gives as comprehensive a coverage as is possible of the present state-of-the-art.
Book Synopsis Modern Analytical Ultracentrifugation by : Todd M. Schuster
Download or read book Modern Analytical Ultracentrifugation written by Todd M. Schuster and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.
Download or read book Fat Injection written by Sydney Coleman and published by Thieme. This book was released on 2017-11-08 with total page 1593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presented by the editors and more than ninety renowned experts on structural fat grafting, this extensively updated volume comprises their state-of-the-art experience and techniques on the use of autologous fat in many aspects of plastic surgery to correct, restore, and enhance patients' structural proportions and defects. Provides a strong foundation of the current Coleman technique of structural fat grafting, including available instrumentation, and the principles and basic concepts of fat injection. Comprehensive coverage on the biology underlying successful fat grafting, as well as excellent color illustrations of the step-by-step technique and descriptions of the effects of the aging process. This new edition contains the most current information on the regenerative potential of grafted fat as well as the long-term results of fat grafting, with updated cases demonstrating the staying power of transferred fat. A remarkable strength of this book is the purposeful inclusion of these worldwide contributors' many different approaches to harvesting, processing, and placing of lipoaspirate, with generous case examples of their results. Clinical applications for all parts of the body, from face and neck to breasts, and upper and lower extremities and genital areas reflect the wide utility of the procedure in reconstructive and aesthetic fields. A chapter on complications and the means to avoid them is given extensive coverage. An e-book and multiple clinical videos are included. This exciting new edition reflects the entire arc of the development of the revolutionary techniques of structural fat grafting, which have sprung from the growing awareness worldwide of the critical role of fat transplantation in aesthetic and reconstructive surgery.
Book Synopsis Principles and Techniques of Biochemistry and Molecular Biology by : Keith Wilson
Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.
