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Cellular Analysis By Atomic Force Microscopy
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Book Synopsis Cellular Analysis by Atomic Force Microscopy by : Malgorzata Lekka
Download or read book Cellular Analysis by Atomic Force Microscopy written by Malgorzata Lekka and published by CRC Press. This book was released on 2017-07-06 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite substantial evidence showing the feasibility of Atomic Force Microscopy (AFM) to identify cells with altered elastic and adhesive properties, the use of this technique as a complementary diagnostic method remains controversial. This book is designed to be a practical textbook that teaches how to assess the mechanical characteristics of living, individual cells by AFM. Following a step-by-step approach, it introduces the methodology of measurements in the case of both determination of elastic properties and quantification of adhesive properties.
Book Synopsis Life at the Nanoscale by : Yves Dufrene
Download or read book Life at the Nanoscale written by Yves Dufrene and published by CRC Press. This book was released on 2019-03-28 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.
Book Synopsis Cellular Analysis by Atomic Force Microscopy by : James Johan Muys
Download or read book Cellular Analysis by Atomic Force Microscopy written by James Johan Muys and published by . This book was released on 2006 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobotics by : Mi Li
Download or read book Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobotics written by Mi Li and published by Springer. This book was released on 2017-10-06 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents methodological and application research in detecting cellular and molecular biophysical properties based on atomic force microscopy (AFM) nanorobotics. Series methods for in situ label-free visualizing and quantifying the multiple physical properties of single cells and single molecules were developed, including immobilization strategies for observing fine structures of living cells, measurements of single-cell mechanics, force recognition of molecular interactions, and mapping protein organizations on cell surface. The biomedical applications of these methods in clinical lymphoma treatments were explored in detail, including primary sample preparation, cancer cell recognition, AFM detection and data analysis. Future directions about the biomedical applications of AFM are also given.
Book Synopsis Atomic Force Microscopy by : Ahmed Touhami
Download or read book Atomic Force Microscopy written by Ahmed Touhami and published by Morgan & Claypool Publishers. This book was released on 2020-05-11 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last two decades, Atomic Force Microscopy (AFM) has undoubtedly had a considerable impact in unraveling the structures and dynamics of microbial surfaces with nanometer resolution, and under physiological conditions. Moreover, the continuous innovations in AFM-based modalities as well as the combination of AFM with modern optical techniques, such as confocal fluorescence microscopy or Raman spectroscopy, increased the diversity and volume of data that can be acquired in an experiment. It is evident that these combinations provide new ways to investigate a broad spectrum of microbiological processes at the level of single cells. In this book, I have endeavored to highlight the wealth of AFM-based modalities that have been implemented over the recent years leading to the multiparametric and multifunctional characterization of, specifically, bacterial surfaces. Examples include the real-time imaging of the nanoscale organization of cell walls, the quantification of subcellular chemical heterogeneities, the mapping and functional analysis of individual cell wall constituents, and the probing of the nanomechanical properties of living bacteria. It is expected that in the near future more AFM-based modalities and complementary techniques will be combined into single experiments to address pertinent problems and challenges in microbial research. Such improvements may make it possible to address the dynamic nature of many more microbial cell surfaces and their constituents, including the restructuring of cellular membranes, pores and transporters, signaling of cell membrane receptors, and formation of cell-adhesion complexes. Ultimately, manifold discoveries and engineering possibilities will materialize as multiparametric tools allow systems of increasing complexity to be probed and manipulated.
Book Synopsis Atomic Force Microscopy in Process Engineering by : W. Richard Bowen
Download or read book Atomic Force Microscopy in Process Engineering written by W. Richard Bowen and published by Butterworth-Heinemann. This book was released on 2009-06-30 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to bring together both the basic theory and proven process engineering practice of AFM. It is presented in a way that is accessible and valuable to practising engineers as well as to those who are improving their AFM skills and knowledge, and to researchers who are developing new products and solutions using AFM. The book takes a rigorous and practical approach that ensures it is directly applicable to process engineering problems. Fundamentals and techniques are concisely described, while specific benefits for process engineering are clearly defined and illustrated. Key content includes: particle-particle, and particle-bubble interactions; characterization of membrane surfaces; the development of fouling resistant membranes; nanoscale pharmaceutical analysis; nanoengineering for cellular sensing; polymers on surfaces; micro and nanoscale rheometry. Atomic force microscopy (AFM) is an important tool for process engineers and scientists as it enables improved processes and products The only book dealing with the theory and practical applications of atomic force microscopy in process engineering Provides best-practice guidance and experience on using AFM for process and product improvement
Book Synopsis Atomic Force Microscopy in Biomedical Research by : Pier Carlo Braga
Download or read book Atomic Force Microscopy in Biomedical Research written by Pier Carlo Braga and published by Humana Press. This book was released on 2011-05-20 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.
Book Synopsis Single Cell Analysis Using Atomic Force Microscopy (AFM) by : Fahmi B. Samsuri
Download or read book Single Cell Analysis Using Atomic Force Microscopy (AFM) written by Fahmi B. Samsuri and published by . This book was released on 2010 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Atomic force microscopy and spectroscopy analysis of membrane protein stability and conformation in cellular bilayer mimics by : Anna Bronder
Download or read book Atomic force microscopy and spectroscopy analysis of membrane protein stability and conformation in cellular bilayer mimics written by Anna Bronder and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Atomic Force Microscopy (AFM) for Measurement of Cell Elastic Properties by : Yi Zhu
Download or read book Atomic Force Microscopy (AFM) for Measurement of Cell Elastic Properties written by Yi Zhu and published by . This book was released on 2015 with total page 73 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis presents data on the use of atomic force microscopy (AFM) for manipulating, detecting, and resolving the mechanical characteristics of individual cells. In this thesis research, AFM was applied to measure the elasticity and adhesive characteristics of a vascular smooth muscle cells (VSMC). The advantage of high-sensitivity (sub-nano-Newton) and high-resolution (sub-micron) makes AFM to be a powerful tool for cell studies. In these studies an AFM nano-indentation protocol was used with extracellular matrix (ECM) biofunctionalized AFM probes to evaluate both cell stiffness and ECM adhesion simultaneously under physiological conditions in a liquid environment. To characterize the cell's mechanical resistance to compression forces applied with AFM probe the Young's modulus (in cellular mechanical properties' research also called elasticity modulus) that is based on Hertz theory (classical infinitesimal strain theory) was used to calculate elastic properties/stiffness of individual VSMC. Measurement of forces required to rupture bonds between the VSMC and ECM coated AFM probe were measured to evaluate cell adhesive properties. VSMC are the major cellular component of the vascular wall and bears external mechanical forces that are caused by blood flow and pressure. The research here described investigates the individual vascular smooth muscle cell (VSMC) cellular and molecular mechanisms to determine how the mechanical and adhesive properties of the VSMC change in aging and hypertension. The hypothesis tested was that both aging and hypertension would increase the stiffness and adhesion of VSMC. The research reports the novel findings that VSMC stiffness and adhesion to the ECM is significantly increased in cells from older monkeys compared to their younger counterparts. An important feature of this research is the use of non-human primate model (monkeys) because of their genetic proximity to humans and its relatively long life-span in comparison to rats or mice. Hypertension is a common age-related vascular disease, in this research a novel spectral analysis approach that revealed oscillation in the underlying cellular and molecular mechanisms of controlling cell stiffness and adhesion, was undertaken to real-timely and dynamically examine and develop the mechanical behavior and action of single VSMC. Moreover, the molecular mechanisms of this dynamic behavior was explored (e.g. Ca2+) to attempt to understand the underlying signaling driving these oscillatory events. In summary, the research demonstrates that age-increased vascular stiffness or hypertension involves not only to alternations in vascular wall ECM, as commonly believed, but also to intrinsic changes in the mechanical stiffness and adhesion of VSMC. This research extends our knowledge of vascular stiffness and contributes some novel findings that may lead to new treatments of vascular diseases associated with vascular stiffening.
Download or read book Force Microscopy written by Bhanu P. Jena and published by Wiley-Liss. This book was released on 2006-07-18 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides a detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function.
Book Synopsis Atomic Force Microscopy by : Pier Carlo Braga
Download or read book Atomic Force Microscopy written by Pier Carlo Braga and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).
Book Synopsis Open-Space Microfluidics by : Emmanuel Delamarche
Download or read book Open-Space Microfluidics written by Emmanuel Delamarche and published by John Wiley & Sons. This book was released on 2018-01-18 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.
Book Synopsis Atomic Force Microscopy by : Ahmed Touhami
Download or read book Atomic Force Microscopy written by Ahmed Touhami and published by Springer Nature. This book was released on 2022-06-01 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last two decades, Atomic Force Microscopy (AFM) has undoubtedly had a considerable impact in unraveling the structures and dynamics of microbial surfaces with nanometer resolution, and under physiological conditions. Moreover, the continuous innovations in AFM-based modalities as well as the combination of AFM with modern optical techniques, such as confocal fluorescence microscopy or Raman spectroscopy, increased the diversity and volume of data that can be acquired in an experiment. It is evident that these combinations provide new ways to investigate a broad spectrum of microbiological processes at the level of single cells. In this book, I have endeavored to highlight the wealth of AFM-based modalities that have been implemented over the recent years leading to the multiparametric and multifunctional characterization of, specifically, bacterial surfaces. Examples include the real-time imaging of the nanoscale organization of cell walls, the quantification of subcellular chemical heterogeneities, the mapping and functional analysis of individual cell wall constituents, and the probing of the nanomechanical properties of living bacteria. It is expected that in the near future more AFM-based modalities and complementary techniques will be combined into single experiments to address pertinent problems and challenges in microbial research. Such improvements may make it possible to address the dynamic nature of many more microbial cell surfaces and their constituents, including the restructuring of cellular membranes, pores and transporters, signaling of cell membrane receptors, and formation of cell-adhesion complexes. Ultimately, manifold discoveries and engineering possibilities will materialize as multiparametric tools allow systems of increasing complexity to be probed and manipulated.
Book Synopsis Stress Relaxation Testing by : Alfred Fox
Download or read book Stress Relaxation Testing written by Alfred Fox and published by ASTM International. This book was released on 1979 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Atomic Force Microscopy/Scanning Tunneling Microscopy by : M.T. Bray
Download or read book Atomic Force Microscopy/Scanning Tunneling Microscopy written by M.T. Bray and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first U. S. Army Natick Research, Development and Engineering Center Atomic Force/Scanning Tunneling Microscopy (AFM/STM) Symposium was held on lune 8-10, 1993 in Natick, Massachusetts. This book represents the compilation of the papers presented at the meeting. The purpose ofthis symposium was to provide a forum where scientists from a number of diverse fields could interact with one another and exchange ideas. The various topics inc1uded application of AFM/STM in material sciences, polymers, physics, biology and biotechnology, along with recent developments inc1uding new probe microscopies and frontiers in this exciting area. The meeting's format was designed to encourage communication between members of the general scientific community and those individuals who are at the cutting edge of AFM, STM and other probe microscopies. It immediately became clear that this conference enabled interdisciplinary interactions among researchers from academia, industry and government, and set the tone for future collaborations. Expert scientists from diverse scientific areas including physics, chemistry, biology, materials science and electronics were invited to participate in the symposium. The agenda of the meeting was divided into three major sessions. In the first session, Biological Nanostructure, topics ranged from AFM ofDNA to STM imagmg ofthe biomoleeule tubulin and bacterialluciferase to the AFM of starch polymer double helices to AFM imaging of food surfaces.
Book Synopsis Practical Cell Analysis by : Dimitri Pappas
Download or read book Practical Cell Analysis written by Dimitri Pappas and published by John Wiley & Sons. This book was released on 2010-02-02 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be “common knowledge” to one field but are often hidden or unknown to another. Within this practical guide: The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented. Elements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis The main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells Many diagram-based protocols for some of the more common cell processes are included Chapter summaries and extensive tables are included so that key information can be looked up easily in the lab setting Much like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis.
