Cartilage Tissue Engineering

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Cartilage Tissue Engineering and Regeneration Techniques

Author : Dimitrios D. Nikolopoulos
Publisher : BoD – Books on Demand
ISBN 13 : 1789238498
Total Pages : 160 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Cartilage Tissue Engineering and Regeneration Techniques by : Dimitrios D. Nikolopoulos

Download or read book Cartilage Tissue Engineering and Regeneration Techniques written by Dimitrios D. Nikolopoulos and published by BoD – Books on Demand. This book was released on 2019-06-05 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: Osteochondral defects can be challenging to treat, first, because the damaged articular cartilage has a poor intrinsic reparative capability, and second, because these defects cause chronic pain and serious disability. That is why cartilage repair remains one of the most challenging issues of musculoskeletal medicine. Arthroscopic and open techniques that have been developed over the last two decades intend to promote the success of complete repair of the articular cartilage defects; nevertheless, these therapies cannot always offer 100% success. Nowadays, cartilage tissue engineering is an emerging technique for the regeneration of cartilage tissue. Taking into consideration these perspectives, this book aims to present a summary of cartilage tissue engineering, including development, recent progress, and major steps taken toward the regeneration of functional cartilage tissue. Special emphasis is placed on the role of stimulating factors, including growth factors, gene therapies, as well as scaffolds, including natural, synthetic, and nanostructured.

Articular Cartilage Tissue Engineering

Author : Kyriacos A. Athanasiou
Publisher : Morgan & Claypool Publishers
ISBN 13 : 1598298755
Total Pages : 183 pages
Book Rating : 4.5/5 (982 download)

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Book Synopsis Articular Cartilage Tissue Engineering by : Kyriacos A. Athanasiou

Download or read book Articular Cartilage Tissue Engineering written by Kyriacos A. Athanasiou and published by Morgan & Claypool Publishers. This book was released on 2010 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cartilage injuries in children and adolescents are increasingly observed, with roughly 20% of knee injuries in adolescents requiring surgery. In the US alone, costs of osteoarthritis (OA) are in excess of $65 billion per year (both medical costs and lost wages). Comorbidities are common with OA and are also costly to manage. Articular cartilage's low friction and high capacity to bear load makes it critical in the movement of one bone against another, and its lack of a sustained natural healing response has necessitated a plethora of therapies. Tissue engineering is an emerging technology at the threshold of translation to clinical use. Replacement cartilage can be constructed in the laboratory to recapitulate the functional requirements of native tissues. This book outlines the biomechanical and biochemical characteristics of articular cartilage in both normal and pathological states, through development and aging. It also provides a historical perspective of past and current cartilage treatments and previous tissue engineering efforts. Methods and standards for evaluating the function of engineered tissues are discussed, and current cartilage products are presented with an analysis on the United States Food and Drug Administration regulatory pathways that products must follow to market. This book was written to serve as a reference for researchers seeking to learn about articular cartilage, for undergraduate and graduate level courses, and as a compendium of articular cartilage tissue engineering design criteria. Table of Contents: Hyaline Articular Cartilage / Cartilage Aging and Pathology / In Vitro / Bioreactors / Future Directions

Bone and Cartilage Engineering

Author : Ulrich Meyer
Publisher : Springer Science & Business Media
ISBN 13 : 3540336060
Total Pages : 272 pages
Book Rating : 4.5/5 (43 download)

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Book Synopsis Bone and Cartilage Engineering by : Ulrich Meyer

Download or read book Bone and Cartilage Engineering written by Ulrich Meyer and published by Springer Science & Business Media. This book was released on 2006-08-02 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bone and Cartilage Engineering provides a complete overview of recent knowledge in bone and cartilage tissue engineering. It follows a logical approach to the various aspects of extracorporal bone and cartilage tissue engineering. The cooperation between a basic scientist and a clinician made it possible to structure the book's content and style according to the interdisciplinary character of the field. The comprehensive nature of the book, including detailed descriptions of laboratory procedures, preclinical approaches, clinical applications, and regulatory issues, will make it an invaluable basis for everyone working in this field. This book will serve as a fundamental tool for basic researchers to establish or refine tissue engineering techniques as well as for clinicians to understand and use this modern therapeutic option.

Cartilage Tissue Engineering

Author : Martin J. Stoddart
Publisher : Springer Nature
ISBN 13 : 1071628399
Total Pages : 392 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Cartilage Tissue Engineering by : Martin J. Stoddart

Download or read book Cartilage Tissue Engineering written by Martin J. Stoddart and published by Springer Nature. This book was released on 2022-11-10 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cartilage Tissue Engineering serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair.

A Tissue Regeneration Approach to Bone and Cartilage Repair

Author : Hala Zreiqat
Publisher : Springer
ISBN 13 : 3319132660
Total Pages : 257 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis A Tissue Regeneration Approach to Bone and Cartilage Repair by : Hala Zreiqat

Download or read book A Tissue Regeneration Approach to Bone and Cartilage Repair written by Hala Zreiqat and published by Springer. This book was released on 2014-12-05 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reviewing exhaustively the current state of the art of tissue engineering strategies for regenerating bones and joints through the use of biomaterials, growth factors and stem cells, along with an investigation of the interactions between biomaterials, bone cells, growth factors and added stem cells and how together skeletal tissues can be optimised, this book serves to highlight the importance of biomaterials composition, surface topography, architectural and mechanical properties in providing support for tissue regeneration. Maximizing reader insights into the importance of the interplay of these attributes with bone cells (osteoblasts, osteocytes and osteoclasts) and cartilage cells (chondrocytes), this book also provides a detailed reference as to how key signalling pathways are activated. The contribution of growth factors to drive tissue regeneration and stem cell recruitment is discussed along with a review the potential and challenges of adult or embryonic mesenchymal stem cells to further enhance the formation of new bone and cartilage tissues. This book serves to demonstrate the interconnectedness of biomaterials, bone/cartilage cells, growth factors and stem cells in determining the regenerative process and thus the clinical outcome.

Tissue Engineering of Cartilage and Bone

Author : Gregory R. Bock
Publisher : John Wiley & Sons
ISBN 13 : 0470864230
Total Pages : 262 pages
Book Rating : 4.4/5 (78 download)

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Book Synopsis Tissue Engineering of Cartilage and Bone by : Gregory R. Bock

Download or read book Tissue Engineering of Cartilage and Bone written by Gregory R. Bock and published by John Wiley & Sons. This book was released on 2003-07-22 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering takes advantages of the combined use of cultured living cells and three-dimensional scaffolds to reconstruct adult tissues that are absent or malfunctioning. This book brings together scientists and clinicians working on a variety of approaches for regenerating of damaged or lost cartilage and bone to assess the progress of this dynamic field. In its early days, tissue engineering was driven by material scientists who designed novel bio-resorbable scaffolds on which to seed cells and grow tissues. This ground-breaking work generated high expectations, but there have been significant stumbling blocks holding back the widespread use of these techniques in the clinic. These challenges, and potential ways of overcoming them, are given thorough coverage in the discussions that follow each chapter. The key questions addressed in this book include the following. How good must cartilage repair be for it to be worthwhile? What is the best source of cells for tissue engineering of both bone and cartilage? Which are the most effective cell scaffolds? What are the best preclinical models for these technologies? And when it comes to clinical trials, what sort of outcome measures should be used? With contributions from some of the leading experts in this field, this timely publication will prove essential reading for anyone with an interest in the field of tissue engineering.

Advances in Biomechanics and Tissue Regeneration

Author : Mohamed Hamdy Doweidar
Publisher : Academic Press
ISBN 13 : 012816610X
Total Pages : 430 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Advances in Biomechanics and Tissue Regeneration by : Mohamed Hamdy Doweidar

Download or read book Advances in Biomechanics and Tissue Regeneration written by Mohamed Hamdy Doweidar and published by Academic Press. This book was released on 2019-08-13 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biomechanics and Tissue Regeneration covers a wide range of recent development and advances in the fields of biomechanics and tissue regeneration. It includes computational simulation, soft tissues, microfluidics, the cardiovascular system, experimental methods in biomechanics, mechanobiology and tissue regeneration. The state-of-the-art, theories and application are presented, making this book ideal for anyone who is deciding which direction to take their future research in this field. In addition, it is ideal for everyone who is exploring new fields or currently working on an interdisciplinary project in tissue biomechanics. Combines new trends in biomechanical modelling and tissue regeneration Offers a broad scope, covering the entire field of tissue biomechanics Contains perspectives from engineering, medicine and biology, thus giving a holistic view of the field

Tissue Engineering Using Ceramics and Polymers

Author : Aldo R. Boccaccini
Publisher : Elsevier
ISBN 13 : 1845693817
Total Pages : 624 pages
Book Rating : 4.8/5 (456 download)

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Book Synopsis Tissue Engineering Using Ceramics and Polymers by : Aldo R. Boccaccini

Download or read book Tissue Engineering Using Ceramics and Polymers written by Aldo R. Boccaccini and published by Elsevier. This book was released on 2007-10-31 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Technology and research in the field of tissue engineering has drastically increased within the last few years to the extent that almost every tissue and organ of the human body could potentially be regenerated. With its distinguished editors and international team of contributors, Tissue Engineering using Ceramics and Polymers reviews the latest research and advances in this thriving area and how they can be used to develop treatments for disease states. Part one discusses general issues such as ceramic and polymeric biomaterials, scaffolds, transplantation of engineered cells, surface modification and drug delivery. Later chapters review characterisation using x-ray photoelectron spectroscopy and secondary ion mass spectrometry as well as environmental scanning electron microscopy and Raman micro-spectroscopy. Chapters in part two analyse bone regeneration and specific types of tissue engineering and repair such as cardiac, intervertebral disc, skin, kidney and bladder tissue. The book concludes with the coverage of themes such as nerve bioengineering and the micromechanics of hydroxyapatite-based biomaterials and tissue scaffolds. Tissue Engineering using Ceramics and Polymers is an innovative reference for professionals and academics involved in the field of tissue engineering. An innovative and up-to-date reference for professionals and academics Environmental scanning electron microscopy is discussed Analyses bone regeneration and specific types of tisue engineering

Cartilage Regeneration

Author : Yunfeng Lin
Publisher : Humana Press
ISBN 13 : 3319516175
Total Pages : 97 pages
Book Rating : 4.3/5 (195 download)

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Book Synopsis Cartilage Regeneration by : Yunfeng Lin

Download or read book Cartilage Regeneration written by Yunfeng Lin and published by Humana Press. This book was released on 2017-02-21 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on cartilage defects and new mesenchymal stem cell-based treatments for their repair and regeneration. Early chapters provide a review of current etiological findings and repair methods of cartilage defects. The next chapters discuss fundamental concepts and features of MSCs, including their proliferation, differentiation, migration and immunomodulatory effects. The discussion also includes clinical applications of MSCs in cartilage tissues, especially with regards to various animal models, biomaterials and transferring techniques. Cartilage Regeneration focuses on the biology of MSCs and their possible applications in cartilage reconstruction, with the goal of bringing new insights into regenerative medicine. It will be essential reading for researchers and clinicians in stem cells, regenerative medicine, biomedical engineering and orthopedic surgery.

Mesenchymal Stem Cells

Author : Phuc Van Pham
Publisher : BoD – Books on Demand
ISBN 13 : 9535136151
Total Pages : 302 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Mesenchymal Stem Cells by : Phuc Van Pham

Download or read book Mesenchymal Stem Cells written by Phuc Van Pham and published by BoD – Books on Demand. This book was released on 2017-11-29 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesenchymal Stem Cells: Isolation, Characterization, and Applications thoroughly presents the isolation, characterization, and some applications of mesenchymal stem cells in the clinic. The book has two parts: "Isolation and Characterization" and "Clinical Perspectives and Applications." In Part I, the subsequent chapters introduce some techniques in isolation, characterization, and purification of mesenchymal stem cells in different tissues. In Part II, some applications of mesenchymal stem cells in the popular diseases, which include cartilage regeneration, spinal cord injury, and osteoarthritis, are discussed. This book provides a succinct yet comprehensive overview of mesenchymal stem cells for advanced students, graduate students, and researchers.

Computational Modeling in Tissue Engineering

Author : Liesbet Geris
Publisher : Springer Science & Business Media
ISBN 13 : 3642325637
Total Pages : 438 pages
Book Rating : 4.6/5 (423 download)

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Book Synopsis Computational Modeling in Tissue Engineering by : Liesbet Geris

Download or read book Computational Modeling in Tissue Engineering written by Liesbet Geris and published by Springer Science & Business Media. This book was released on 2012-10-30 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the major challenges in tissue engineering is the translation of biological knowledge on complex cell and tissue behavior into a predictive and robust engineering process. Mastering this complexity is an essential step towards clinical applications of tissue engineering. This volume discusses computational modeling tools that allow studying the biological complexity in a more quantitative way. More specifically, computational tools can help in: (i) quantifying and optimizing the tissue engineering product, e.g. by adapting scaffold design to optimize micro-environmental signals or by adapting selection criteria to improve homogeneity of the selected cell population; (ii) quantifying and optimizing the tissue engineering process, e.g. by adapting bioreactor design to improve quality and quantity of the final product; and (iii) assessing the influence of the in vivo environment on the behavior of the tissue engineering product, e.g. by investigating vascular ingrowth. The book presents examples of each of the above mentioned areas of computational modeling. The underlying tissue engineering applications will vary from blood vessels over trachea to cartilage and bone. For the chapters describing examples of the first two areas, the main focus is on (the optimization of) mechanical signals, mass transport and fluid flow encountered by the cells in scaffolds and bioreactors as well as on the optimization of the cell population itself. In the chapters describing modeling contributions in the third area, the focus will shift towards the biology, the complex interactions between biology and the micro-environmental signals and the ways in which modeling might be able to assist in investigating and mastering this complexity. The chapters cover issues related to (multiscale/multiphysics) model building, training and validation, but also discuss recent advances in scientific computing techniques that are needed to implement these models as well as new tools that can be used to experimentally validate the computational results.

Cartilage Tissue Engineering

Author : Pauline M. Doran
Publisher :
ISBN 13 : 9781493929382
Total Pages : 290 pages
Book Rating : 4.9/5 (293 download)

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Book Synopsis Cartilage Tissue Engineering by : Pauline M. Doran

Download or read book Cartilage Tissue Engineering written by Pauline M. Doran and published by . This book was released on 2015 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues.

Bone and Cartilage Regeneration

Author : Phuc Van Pham
Publisher : Springer
ISBN 13 : 3319401440
Total Pages : 315 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Bone and Cartilage Regeneration by : Phuc Van Pham

Download or read book Bone and Cartilage Regeneration written by Phuc Van Pham and published by Springer. This book was released on 2017-01-24 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This invaluable resource discusses clinical applications with effects and side-effects of applications of stem cells in bone and cartilage regeneration. Each chapter is contributed by a pre-eminent scientist in the field and covers such topics as skeletal regeneration by mesenchymal stem cells, clinical improvement of mesenchymal stem cell injection in injured cartilage and osteoarthritis, Good manufacturing practice (GMP), minimal critera of stem cells for clinical applications, future directions of the discussed therapies and much more. Bone & Cartilage Regeneration and the other books in the Stem Cells in Clinical Applications series will be invaluable to scientists, researchers, advanced students and clinicians working in stem cells, regenerative medicine or tissue engineering.

Cartilage: From Biology to Biofabrication

Author : Mohamadreza Baghaban Eslaminejad
Publisher : Springer Nature
ISBN 13 : 9819924529
Total Pages : 531 pages
Book Rating : 4.8/5 (199 download)

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Book Synopsis Cartilage: From Biology to Biofabrication by : Mohamadreza Baghaban Eslaminejad

Download or read book Cartilage: From Biology to Biofabrication written by Mohamadreza Baghaban Eslaminejad and published by Springer Nature. This book was released on 2023-07-11 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of cartilage structure, functions, and approaches for the regeneration of cartilage tissue. It reviews multiple signaling pathways that are involved in the growth and repair of cartilage tissue. The initial chapter of the book examines the etiology, diagnosis, and pathological features of various cartilage diseases. Subsequently, the book presents recent advances in biomaterial sciences, regenerative medicine, and fabrication technology for cartilage regeneration. It discusses hydrogels as a promising scaffold for cartilage tissue engineering, focusing on recapitulating microenvironments present during development or in adult tissue to induce the formation of cartilaginous constructs with biochemical and mechanical properties of native tissue. Lastly, it covers the applications of 3 D printing techniques for the fabrication of scaffolds for cartilage tissue regeneration for the production of biological implants capable of treating a range of conditions.

Culture of Cells for Tissue Engineering

Author : Gordana Vunjak-Novakovic
Publisher : John Wiley & Sons
ISBN 13 : 0471741809
Total Pages : 528 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Culture of Cells for Tissue Engineering by : Gordana Vunjak-Novakovic

Download or read book Culture of Cells for Tissue Engineering written by Gordana Vunjak-Novakovic and published by John Wiley & Sons. This book was released on 2006-03-31 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step, practicalguidance for the acquisition, manipulation, and use of cell sourcesfor tissue engineering. It offers a unique focus on tissueengineering methods for cell sourcing and utilization, combiningtheoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing,preparation, and characterization and the second part examinesspecific engineered tissues Each chapter covers: structural and functional properties oftissues, methodological principles, culture, cellselection/expansion, cell modifications, cell seeding, tissueculture, analytical assays, and a detailed description ofrepresentative studies End-of-chapter features include useful listings of sources forreagents, materials, and supplies, with the contact details of thesuppliers listed at the end of the book A section of elegant color plates to back up the figures in thechapters Culture of Cells for Tissue Engineering givesnovice and seasoned researchers in tissue engineering an invaluableresource. In addition, the text is suitable for professionals inrelated research, particularly in those areas where cell and tissueculture is a new or emerging tool.

Articular Cartilage

Author : Kyriacos A. Athanasiou
Publisher : CRC Press
ISBN 13 : 143985324X
Total Pages : 442 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Articular Cartilage by : Kyriacos A. Athanasiou

Download or read book Articular Cartilage written by Kyriacos A. Athanasiou and published by CRC Press. This book was released on 2013-03-21 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Well-known for their inability to heal, articular cartilage injuries often degenerate inexorably to disastrous impairment. Multitudes of treatments have been devised for this problem, but no satisfactory long-term solutions have been established. Written by world-class experts, Articular Cartilage covers the latest research and advancements related to biology, development, pathology, clinical applications, and tissue engineering. This book is useful for rheumatologists, orthopaedic surgeons, cartilage biologists, and cartilage engineers as well as for professionals working in the orthopaedic and other musculoskeletal industries. This book also belongs in the library of primary care physicians, gerontologists, physical therapists, kinesiologists, and chiropractors. Written at a level that allows accessibility to a wide audience, it provides an interdisciplinary approach that encompasses the breadth and depth of basic science, bioengineering, translational science, and detailed methodologic approaches. The authors examine the major events and signaling molecules that lead to development of articular cartilage from precursor cells, and the changes in cartilage as it matures and ages. They focus on the epidemiology, etiopathogenesis, and therapeutic approaches for cartilage injury and the major arthritides that affect cartilage and the synovial joints such as osteoarthritis, rheumatoid arthritis, and gout. They supply an up-to-date overview of the field of tissue engineering as applied to articular cartilage repair. They examine a number of methods used to assess structure, composition, biology, and biomechanical function. Each chapter contains extensive references to enhance additional study. The book’s comprehensive focus on multiple aspects of articular cartilage sets it apart from other tissue engineering or developmental biology-based books available. It includes important discussions and perspectives on many of the remaining challenges and opportunities in the development and translation of new approaches for treating diseases of articular cartilage. It also provides detailed working protocols for many of the methods used to study articular cartilage, coverage of current treatment options, and business and regulatory aspects of the development of cartilage products. It provides a deeper understanding that will help with the development of new products and clinical applications.

Nanoengineering Materials for Biomedical Uses

Author : Emilio I. Alarcon
Publisher : Springer Nature
ISBN 13 : 3030312615
Total Pages : 208 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis Nanoengineering Materials for Biomedical Uses by : Emilio I. Alarcon

Download or read book Nanoengineering Materials for Biomedical Uses written by Emilio I. Alarcon and published by Springer Nature. This book was released on 2019-11-02 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book fills the gap between fundamental and applied research in the use of nanomaterials in biomedical applications, covering the most relevant areas, such as the fundamental concepts of the preparation of nanostructures and regulatory requirements for their safe use in biomedical devices. It also critically discusses what has been achieved in the field, and what needs to be urgently addressed and reviews the state-of-the-art medical uses of nanomaterials for treating damaged organs and tissues. Combining the expertise of clinical researchers working in the field of tissue engineering and novel materials, the book explores the main topics regarding the characterization of materials, specific organ-oriented biomaterials and their applications, as well as regulations and safety. Further, it also examines recent advances, difficulties, and clinical requirements in terms of human bone, cornea, heart, skin and the nervous system, allowing readers to gain a clear and comprehensive understanding of current nanomaterial use in biomedical applications and devices, together with the challenges and future trends. This book is a valuable tool for multidisciplinary scientists and experts interested in fundamental concepts and synthetic routes for preparing nanomaterials. It is also of interest to students and researchers involved in cross-disciplinary research in nanomaterials for clinical applications and offers practical insights for clinicians as well as engineers and materials scientists working in nanoengineering.