Bone Research Protocols

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Bone Research Protocols

Author : Miep H. Helfrich
Publisher : Springer Science & Business Media
ISBN 13 : 1592593666
Total Pages : 444 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Bone Research Protocols by : Miep H. Helfrich

Download or read book Bone Research Protocols written by Miep H. Helfrich and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).

Bone Research Protocols

Author : Miep H. Helfrich
Publisher : Humana
ISBN 13 : 9781617373213
Total Pages : 0 pages
Book Rating : 4.3/5 (732 download)

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Book Synopsis Bone Research Protocols by : Miep H. Helfrich

Download or read book Bone Research Protocols written by Miep H. Helfrich and published by Humana. This book was released on 2010-11-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).

Bone Research Protocols

Author : Aymen I. Idris
Publisher :
ISBN 13 : 9781493989973
Total Pages : 670 pages
Book Rating : 4.9/5 (899 download)

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Book Synopsis Bone Research Protocols by : Aymen I. Idris

Download or read book Bone Research Protocols written by Aymen I. Idris and published by . This book was released on 2019 with total page 670 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Osteoporosis

Author : Jennifer J. Westendorf
Publisher : Humana Press
ISBN 13 : 9781627038478
Total Pages : 0 pages
Book Rating : 4.0/5 (384 download)

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Book Synopsis Osteoporosis by : Jennifer J. Westendorf

Download or read book Osteoporosis written by Jennifer J. Westendorf and published by Humana Press. This book was released on 2014-11-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In this book, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. This is the perfect guide for both new and experienced experimentalists attempting to study osteoporosis.

Osteoporosis

Author : Jennifer J. Westendorf
Publisher : Humana Press
ISBN 13 : 9781588298287
Total Pages : 354 pages
Book Rating : 4.2/5 (982 download)

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Book Synopsis Osteoporosis by : Jennifer J. Westendorf

Download or read book Osteoporosis written by Jennifer J. Westendorf and published by Humana Press. This book was released on 2008-04-16 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In this book, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. This is the perfect guide for both new and experienced experimentalists attempting to study osteoporosis.

Bone Research in Biomechanics

Author : G. Lowet
Publisher : IOS Press
ISBN 13 : 9789051993271
Total Pages : 234 pages
Book Rating : 4.9/5 (932 download)

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Book Synopsis Bone Research in Biomechanics by : G. Lowet

Download or read book Bone Research in Biomechanics written by G. Lowet and published by IOS Press. This book was released on 1997 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the structure of bone, and its consequences for the mechanical behaviour of the bone structure. The first part of this book focuses on the development of models to predict the adaptation of bone due to changes on the mechanical loading situation (such as provoked by an implant). But far more important than the computer power presently available, the incorporation of knowledge on the biological processes have led to new kinds of models. Next to the development of models itself, the issue of model validation though comparison with clinical data is a main issue addressed in the papers of this symposium. The second part, dealing with the relationship between bone architecture and competence of bone, focuses on the morphology of trabecular bone structure. This work is mainly carried out in the context of research on osteoporosis, and look for the relation between bone structure and fracture risk. The last part is devoted to ultrasound research in bone biomechanics. Several methods have been described for the in vitro and in vivo measurement of ultrasound velocity and attenuation, both on cortical and on trabecular bone. The reader will not only discover the state-of-the-art when reading though this book. This book can give a taste of the fascinating perspectives the research in bone biomechanics still have to offer, even after more than 100 years.

Osteoporosis and Osteoarthritis

Author : Jennifer J. Westendorf
Publisher :
ISBN 13 : 9781493916191
Total Pages : 214 pages
Book Rating : 4.9/5 (161 download)

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Book Synopsis Osteoporosis and Osteoarthritis by : Jennifer J. Westendorf

Download or read book Osteoporosis and Osteoarthritis written by Jennifer J. Westendorf and published by . This book was released on 2014 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Osteoporosis and Osteoarthritis expert investigators detail modern tools to enhance discovery in bone and cartilage biology and promote translational research in osteoporosis and osteoarthritis. New chapters describe methods to generate induced pluripotent stem cells, perform high-throughput chromatin analysis, assess loads on bone cells in vivo and in vitro, use state-of-the art imaging techniques to measure articular cartilage thickness and biochemical properties, and establish animal models of osteoarthritis and bone healing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Osteoporosis and Osteoarthritis shares successful techniques that can be applied to the study of either disease.

Clinical Trials in Osteoporosis

Author : Derek Pearson
Publisher : Springer Science & Business Media
ISBN 13 : 1447137108
Total Pages : 280 pages
Book Rating : 4.4/5 (471 download)

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Book Synopsis Clinical Trials in Osteoporosis by : Derek Pearson

Download or read book Clinical Trials in Osteoporosis written by Derek Pearson and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical Trials in Osteoporosis is a practical handbook on clinical trials in the growing field of osteoporosis. Topics covered include study design, technical issues, data collection, quality assurance, data analysis and presentation. It aims to take the user through the process step-by-step from start to finish, also providing a background on regulatory guidelines, ethical implications, endpoints, current therapies and the ideal drug to use. There are no other books at present that specifically address the issue of clinical trials in osteoporosis. A number of issues dealt with in this book have been brought together in one publication for the first time. Clinical Trials in Osteoporosis is intended to serve as a practical manual for clinicians and scientists coming to the subject new and to provide a standard for existing centers to measure themselves against.

Osteoporosis and Osteoarthritis

Author : Andre J. van Wijnen
Publisher :
ISBN 13 : 9781071609910
Total Pages : pages
Book Rating : 4.6/5 (99 download)

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Book Synopsis Osteoporosis and Osteoarthritis by : Andre J. van Wijnen

Download or read book Osteoporosis and Osteoarthritis written by Andre J. van Wijnen and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Guidelines for Preclinical Evaluation and Clinical Trials in Osteoporosis

Author : World Health Organization
Publisher :
ISBN 13 : 9789241545228
Total Pages : 68 pages
Book Rating : 4.5/5 (452 download)

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Book Synopsis Guidelines for Preclinical Evaluation and Clinical Trials in Osteoporosis by : World Health Organization

Download or read book Guidelines for Preclinical Evaluation and Clinical Trials in Osteoporosis written by World Health Organization and published by . This book was released on 1998-01-01 with total page 68 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides comprehensive guidelines for the design, implementation, and interpretation of preclinical studies and clinical trials of agents undergoing investigation for the management of osteoporosis. Noting the magnitude of the public health problem caused by osteoporosis and related fractures, the book responds to the urgent need for a cohesive and rational approach to the search for new therapeutic or preventive agents. Recommended principles and methods reflect the consensus reached by a large number of international experts, representatives of the pharmaceutical industry, and drug regulatory authorities. The book opens with a brief discussion of the current and future public health problem caused by osteoporotic fractures, followed by conceptual and operational definitions of osteoporosis and a recommended classification of four intervention categories that facilitate the definition of treatment objectives and the design of targetted studies. Against this background, the next chapter offers a detailed guide to the aims, design, conduct and interpretation of preclinical studies. Specific recommendations are given for the selection of in vitro and animal models, the design of the study, including dose ranges and duration of treatment, and the most appropriate end-points for assessing efficacy. The most extensive chapter provides a comprehensive description of basic principles and methods to follow during clinical trials. Information ranges from a discussion of problems with the use of bone mineral density to predict fracture risk, through advice on the choice of tests and techniques for measuring the effects of interventions, to a discussion of general principles of trial design, including selection of study population, treatment regimen, duration of studies, sample size, and frequency of measurement. Drawing on these detailed principles and methods, subsequent chapters offer concise guidance specific to phase I and phase II studies, phase III studies in severe osteoporosis, phase III studies in osteoporosis without fragility fractures, and phase III studies in osteopenia. The book concludes with chapters outlining principles and methods for phase III clinical trials in subjects with normal bone mass, and for phase IV studies.

B Cell Protocols

Author : Hua Gu
Publisher : Springer Science & Business Media
ISBN 13 : 1592597963
Total Pages : 294 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis B Cell Protocols by : Hua Gu

Download or read book B Cell Protocols written by Hua Gu and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.

Mesenchymal Stem Cells

Author : Darwin J. Prockop
Publisher : Humana Press
ISBN 13 : 9781588297716
Total Pages : 0 pages
Book Rating : 4.2/5 (977 download)

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Book Synopsis Mesenchymal Stem Cells by : Darwin J. Prockop

Download or read book Mesenchymal Stem Cells written by Darwin J. Prockop and published by Humana Press. This book was released on 2008-03-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In this essential book, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Cutting edge and easy to use, this book is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.

Macrophages

Author : Germain Rousselet
Publisher : Humana
ISBN 13 : 9781493978366
Total Pages : 0 pages
Book Rating : 4.9/5 (783 download)

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Book Synopsis Macrophages by : Germain Rousselet

Download or read book Macrophages written by Germain Rousselet and published by Humana. This book was released on 2018-05-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level. The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com.

Handbook of Histology Methods for Bone and Cartilage

Author : Yuehuei H. An
Publisher : Springer Science & Business Media
ISBN 13 : 1592594174
Total Pages : 582 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Handbook of Histology Methods for Bone and Cartilage by : Yuehuei H. An

Download or read book Handbook of Histology Methods for Bone and Cartilage written by Yuehuei H. An and published by Springer Science & Business Media. This book was released on 2003-05-01 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histotechnology and histomorphometry are the major methodologies in bone and cartila- related research. Handbook of Histology Methods for Bone and Cartilage is an outgrowth of the editors’ own quest for information on bone and cartilage histology and histomorphometry. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and cartilage, orthopedic, or dental research. It is the first inclusive and organized reference book on histological and histomorphometrical techniques on bone and cartilage specimens. The topic has not previously been covered adequately by any existing books in the field. Handbook of Histology Methods for Bone and Cartilage has six major parts and is designed to be concise as well as inclusive, and more practical than theoretical. The text is simple and straightforward. Large numbers of tables, line drawings, and micro- or macro-photographs, are used to help readers better understand the content. Full bibliographies at the end of each chapter guide readers to more detailed information. A book of this length cannot discuss every method for bone and cartilage histology that has been used over the years, but it is hoped that major methods and their applications have been included.

Basic Cell Culture Protocols

Author : Jeffrey W. Pollard
Publisher : Springer Science & Business Media
ISBN 13 : 0896034410
Total Pages : 477 pages
Book Rating : 4.8/5 (96 download)

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Book Synopsis Basic Cell Culture Protocols by : Jeffrey W. Pollard

Download or read book Basic Cell Culture Protocols written by Jeffrey W. Pollard and published by Springer Science & Business Media. This book was released on 1997 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Stem Cell Protocols

Author : Ivan N. Rich
Publisher : Humana Press
ISBN 13 : 9781493917846
Total Pages : 0 pages
Book Rating : 4.9/5 (178 download)

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Book Synopsis Stem Cell Protocols by : Ivan N. Rich

Download or read book Stem Cell Protocols written by Ivan N. Rich and published by Humana Press. This book was released on 2014-11-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.

Craniofacial Development

Author : Sebastian Dworkin
Publisher : Humana
ISBN 13 : 9781071618493
Total Pages : 0 pages
Book Rating : 4.6/5 (184 download)

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Book Synopsis Craniofacial Development by : Sebastian Dworkin

Download or read book Craniofacial Development written by Sebastian Dworkin and published by Humana. This book was released on 2021-12-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques.