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Biological Production Of A Hydrocarbon Fuel Intermediate Polyhydroxybutyrate Phb From A Process Relevant Lignocellulosic Derived Sugar
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Book Synopsis Biological production of a hydrocarbon fuel intermediate polyhydroxybutyrate (PHB) from a process relevant lignocellulosic derived sugar by : Wei Wang
Download or read book Biological production of a hydrocarbon fuel intermediate polyhydroxybutyrate (PHB) from a process relevant lignocellulosic derived sugar written by Wei Wang and published by . This book was released on 2014 with total page 1 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (Phb) from a Process Relevant Lignocellulosic Derived Sugar by :
Download or read book Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (Phb) from a Process Relevant Lignocellulosic Derived Sugar written by and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgraded to hydrocarbon fuels via commercial oligomerization technologies. In recent years a great effort has been made in bacterial production of PHB, yet the production cost of the polymer is still much higher than conventional petrochemical plastics. The high cost of PHB is because the cost of the substrates can account for as much as half of the total product cost in large scale fermentation. Thus searching for cheaper and better substrates is very necessary for PHB production. In this study, we demonstrate production of PHB by Cupriavidus necator from a process relevant lignocellulosic derived sugar stream, i.e., saccharified hydrolysate slurry from pretreated corn stover. Good cell growth was observed on slurry saccharified with advanced enzymes and 40~60% of PHB was accumulated in the cells. The mechanism of inhibition in the toxic hydrolysate generated by pretreatment and saccharification of biomass, will be discussed.
Book Synopsis Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (PHB) from a Process Relevant Lignocellulosic Derived Sugar (Poster). by :
Download or read book Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (PHB) from a Process Relevant Lignocellulosic Derived Sugar (Poster). written by and published by . This book was released on 2014 with total page 1 pages. Available in PDF, EPUB and Kindle. Book excerpt: PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgraded to hydrocarbon fuels via commercial oligomerization technologies. Cupriavidus necator is the microorganism that has been most extensively studied and used for PHB production on an industrial scale; However the substrates used for producing PHB are mainly fructose, glucose, sucrose, fatty acids, glycerol, etc., which are expensive. In this study, we demonstrate production of PHB from a process relevant lignocellulosic derived sugar stream, i.e., saccharified slurry from pretreated corn stover. The strain was first investigated in shake flasks for its ability to utilize glucose, xylose and acetate. In addition, the strain was also grown on pretreated lignocellulose hydrolyzate slurry and evaluated in terms of cell growth, sugar utilization, PHB accumulation, etc. The mechanism of inhibition in the toxic hydrolysate generated by the pretreatment and saccharification process of biomass, was also studied.
Book Synopsis Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (PHB) from a Process Relevant Lignocellulosic Derived Sugar (Poster) by :
Download or read book Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (PHB) from a Process Relevant Lignocellulosic Derived Sugar (Poster) written by and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgradedto hydrocarbon fuels via commercial oligomerization technologies. Cupriavidus necator is the microorganism that has been most extensively studied and used for PHB production on an industrial scale; However the substrates used for producing PHB are mainly fructose, glucose, sucrose, fatty acids, glycerol, etc., which are expensive. In this study, we demonstrate production of PHB from a processrelevant lignocellulosic derived sugar stream, i.e., saccharified slurry from pretreated corn stover. The strain was first investigated in shake flasks for its ability to utilize glucose, xylose and acetate. In addition, the strain was also grown on pretreated lignocellulose hydrolyzate slurry and evaluated in terms of cell growth, sugar utilization, PHB accumulation, etc. The mechanism ofinhibition in the toxic hydrolysate generated by the pretreatment and saccharification process of biomass, was also studied.
Book Synopsis Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons by : Ryan Davis
Download or read book Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons written by Ryan Davis and published by . This book was released on 2013 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report describes one potential conversion process to hydrocarbon products by way of biological conversion of lingnocellulosic-derived sugars. The process design converts biomass to a hydrocarbon intermediate, a free fatty acid, using dilute-acid pretreatment, enzymatic saccharification, and bioconversion. Ancillary areas--feed handling, hydrolysate conditioning, product recovery and upgrading (hydrotreating) to a final blendstock material, wastewater treatment, lignin combustion, and utilities--are also included in the design.
Book Synopsis Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons Via Indirect Liquefaction. Thermochemical Research Pathway to High-Octane Gasoline Blendstock Through Methanol/Dimethyl Ether Intermediates by :
Download or read book Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons Via Indirect Liquefaction. Thermochemical Research Pathway to High-Octane Gasoline Blendstock Through Methanol/Dimethyl Ether Intermediates written by and published by . This book was released on 2015 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report was developed as part of the U.S. Department of Energy's Bioenergy Technologies Office's (BETO's) efforts to enable the development of technologies for the production of infrastructure-compatible, cost-competitive liquid hydrocarbon fuels from lignocellulosic biomass feedstocks. The research funded by BETO is designed to advance the state of technology of biomass feedstock supply and logistics, conversion, and overall system sustainability. It is expected that these research improvements will be made within the 2022 timeframe. As part of their involvement in this research and development effort, the National Renewable Energy Laboratory and the Pacific Northwest National Laboratory investigate the economics of conversion pathways through the development of conceptual biorefinery process models and techno-economic analysis models. This report describes in detail one potential conversion process for the production of high-octane gasoline blendstock via indirect liquefaction of biomass. The processing steps of this pathway include the conversion of biomass to synthesis gas or syngas via indirect gasification, gas cleanup, catalytic conversion of syngas to methanol intermediate, methanol dehydration to dimethyl ether (DME), and catalytic conversion of DME to high-octane, gasoline-range hydrocarbon blendstock product. The conversion process configuration leverages technologies previously advanced by research funded by BETO and demonstrated in 2012 with the production of mixed alcohols from biomass. Biomass-derived syngas cleanup via reforming of tars and other hydrocarbons is one of the key technology advancements realized as part of this prior research and 2012 demonstrations. The process described in this report evaluates a new technology area for the downstream utilization of clean biomass-derived syngas for the production of high-octane hydrocarbon products through methanol and DME intermediates. In this process, methanol undergoes dehydration to DME, which is subsequently converted via homologation reactions to high-octane, gasoline-range hydrocarbon products.
Book Synopsis Lignocellulose Conversion by : Vincenza Faraco
Download or read book Lignocellulose Conversion written by Vincenza Faraco and published by Springer. This book was released on 2013-06-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol has been recognized as a potential alternative to petroleum-derived transportation fuels. Even if cellulosic biomass is less expensive than corn and sugarcane, the higher costs for its conversion make the near-term price of cellulosic ethanol higher than that of corn ethanol and even more than that of sugarcane ethanol. Conventional process for bioethanol production from lignocellulose includes a chemical/physical pre-treatment of lignocellulose for lignin removal, mostly based on auto hydrolysis and acid hydrolysis, followed by saccharification of the free accessible cellulose portions of the biomass. The highest yields of fermentable sugars from cellulose portion are achieved by means of enzymatic hydrolysis, currently carried out using a mix of cellulases from the fungus Trichoderma reesei. Reduction of (hemi)cellulases production costs is strongly required to increase competitiveness of second generation bioethanol production. The final step is the fermentation of sugars obtained from saccharification, typically performed by the yeast Saccharomyces cerevisiae. The current process is optimized for 6-carbon sugars fermentation, since most of yeasts cannot ferment 5-carbon sugars. Thus, research is aimed at exploring new engineered yeasts abilities to co-ferment 5- and 6-carbon sugars. Among the main routes to advance cellulosic ethanol, consolidate bio-processing, namely direct conversion of biomass into ethanol by a genetically modified microbes, holds tremendous potential to reduce ethanol production costs. Finally, the use of all the components of lignocellulose to produce a large spectra of biobased products is another challenge for further improving competitiveness of second generation bioethanol production, developing a biorefinery.
Book Synopsis Adding Value to Lignocellulosic Biorefinery by : Mengxing Li
Download or read book Adding Value to Lignocellulosic Biorefinery written by Mengxing Li and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polyhydroxybutyrate (PHB) is bacteria synthesized polymer that has comparable mechanical properties as petroleum-based plastics and high biocompatibility. Current commercial PHB production process is not cost effective. Raw materials make up about 50% of the production cost. Lignocellulosic biomass are cheap, abundant feedstocks that can be converted into PHB to add profit and sustainability to lignocellulosic biorefinery. Lignocellulosic biorefinery upstream process produces polymeric sugar rich stream and lignin-enriched stream. Polymeric sugars are then hydrolyzed into a sugar stream with glucose, xyloseand arabinose mainly present. To the best of the author's knowledge, limited studies have been done on sugar mixture conversion into PHB. For lignin, previous research achieved a PHB production ranging from 0.13 to 1 g/L, which is too low to be economical. The primary objectives of this research were twofold: (1) process development of polymeric sugars conversion into PHB, with a focus on sugar mixture conversion into PHB by Burkholderia sacchari DSM 17165, and (2) process development of lignin into PHB by Cupriavidus necator DSM 545, with a focus on enhancing PHB production using various types of supplements. For sugar mixture conversion into PHB, first, shake flask (250 mL) scale statistical experimental design and modeling were performed to optimize sugar mixture ratio and process variables for maximal PHB production; second, bioreactor scale (3L) fed-batch cultivation was conducted to produce PHB from simulated corn fiber sugar mixture. The highest PHB production reached 67 g/L for 4:2:1 (glucose:xylose:arabinose) mixture at 41 h corresponding to an accumulation of 77% of cell dry weight. Corresponding sugar conversion efficiency and productivity were 0.33 g PHB/g sugar consumed and 1.6 g/L/h, respectively, which are comparable to or higher than most previous studies. For lignin conversion into PHB, first, shake scale (250 mL) study achieved 10-fold increase (0.2 to 2.1 g/L) in PHB production by optimizing supplement formulations with Plackett-Burman and central composite designs. Second, fed-batch cultivation at bioreactor scale (1.7 L) were conducted to enhance PHB production to 4.5 g/L. This is the highest PHB production from lignin that the author has been aware of in the literature.
Book Synopsis Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons by :
Download or read book Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons written by and published by . This book was released on 2013 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report describes one potential conversion process to hydrocarbon products by way of biological conversion of lingnocellulosic-dervied sugars. The process design converts biomass to a hydrocarbon intermediate, a free fatty acid, using dilute-acid pretreatement, enzymatic saccharification, and bioconversion. Ancillary areas--feed handling, hydrolysate conditioning, product recovery and upgrading (hydrotreating) to a final blendstock material, wastewater treatment, lignin combusion, and utilities--are also included in the design.
Book Synopsis Metabolic Engineering of Zymomonas Mobilis for 2,3-butanediol Production from Lignocellulosic Biomass Sugars by :
Download or read book Metabolic Engineering of Zymomonas Mobilis for 2,3-butanediol Production from Lignocellulosic Biomass Sugars written by and published by . This book was released on 2016 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: To develop pathways for advanced biofuel production, and to understand the impact of host metabolism and environmental conditions on heterologous pathway engineering for economic advanced biofuels production from biomass, we seek to redirect the carbon flow of the model ethanologen Zymomonas mobilis to produce desirable hydrocarbon intermediate 2,3-butanediol (2,3-BDO). 2,3-BDO is a bulk chemical building block, and can be upgraded in high yields to gasoline, diesel, and jet fuel. 2,3-BDO biosynthesis pathways from various bacterial species were examined, which include three genes encoding acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase. Bioinformatics analysis was carried out to pinpoint potential bottlenecks for high 2,3-BDO production. Different combinations of 2,3-BDO biosynthesis metabolic pathways using genes from different bacterial species have been constructed. Our results demonstrated that carbon flux can be deviated from ethanol production into 2,3-BDO biosynthesis, and all three heterologous genes are essential to efficiently redirect pyruvate from ethanol production for high 2,3-BDO production in Z. mobilis. The down-selection of best gene combinations up to now enabled Z. mobilis to reach the 2,3-BDO production of more than 10 g/L from glucose and xylose, as well as mixed C6/C5 sugar streams derived from the deacetylation and mechanical refining process. In conclusion, this study confirms the value of integrating bioinformatics analysis and systems biology data during metabolic engineering endeavors, provides guidance for value-added chemical production in Z. mobilis, and reveals the interactions between host metabolism, oxygen levels, and a heterologous 2,3-BDO biosynthesis pathway. Taken together, this work provides guidance for future metabolic engineering efforts aimed at boosting 2,3-BDO titer anaerobically.
Book Synopsis Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Catalytic Conversion of Sugars to Hydrocarbons by :
Download or read book Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Catalytic Conversion of Sugars to Hydrocarbons written by and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report describes one potential conversion process to hydrocarbon products by way of catalytic conversion of lignocellulosic-derived hydrolysate. This model leverages expertise established over time in biomass deconstruction and process integration research at NREL, while adding in new technology areas for sugar purification and catalysis. The overarching process design converts biomass to diesel- and naphtha-range fuels using dilute-acid pretreatment, enzymatic saccharification, purifications, and catalytic conversion focused on deoxygenating and oligomerizing biomass hydrolysates.
Book Synopsis Biobased Industrial Products by : National Research Council
Download or read book Biobased Industrial Products written by National Research Council and published by National Academies Press. This book was released on 2000-02-16 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Petroleum-based industrial products have gradually replaced products derived from biological materials. However, biologically based products are making a comebackâ€"because of a threefold increase in farm productivity and new technologies. Biobased Industrial Products envisions a biobased industrial future, where starch will be used to make biopolymers and vegetable oils will become a routine component in lubricants and detergents. Biobased Industrial Products overviews the U.S. land resources available for agricultural production, summarizes plant materials currently produced, and describes prospects for increasing varieties and yields. The committee discusses the concept of the biorefinery and outlines proven and potential thermal, mechanical, and chemical technologies for conversion of natural resources to industrial applications. The committee also illustrates the developmental dynamics of biobased products through existing examples, as well as products still on the drawing board, and it identifies priorities for research and development.
Author :National Academies of Sciences, Engineering, and Medicine Publisher :National Academies Press ISBN 13 :0309495679 Total Pages :393 pages Book Rating :4.3/5 (94 download)
Book Synopsis Safeguarding the Bioeconomy by : National Academies of Sciences, Engineering, and Medicine
Download or read book Safeguarding the Bioeconomy written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2020-05-01 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research and innovation in the life sciences is driving rapid growth in agriculture, biomedical science, information science and computing, energy, and other sectors of the U.S. economy. This economic activity, conceptually referred to as the bioeconomy, presents many opportunities to create jobs, improve the quality of life, and continue to drive economic growth. While the United States has been a leader in advancements in the biological sciences, other countries are also actively investing in and expanding their capabilities in this area. Maintaining competitiveness in the bioeconomy is key to maintaining the economic health and security of the United States and other nations. Safeguarding the Bioeconomy evaluates preexisting and potential approaches for assessing the value of the bioeconomy and identifies intangible assets not sufficiently captured or that are missing from U.S. assessments. This study considers strategies for safeguarding and sustaining the economic activity driven by research and innovation in the life sciences. It also presents ideas for horizon scanning mechanisms to identify new technologies, markets, and data sources that have the potential to drive future development of the bioeconomy.
Author :Patrick C. Hallenbeck Publisher :Springer Science & Business Media ISBN 13 :1461412080 Total Pages :262 pages Book Rating :4.4/5 (614 download)
Book Synopsis Microbial Technologies in Advanced Biofuels Production by : Patrick C. Hallenbeck
Download or read book Microbial Technologies in Advanced Biofuels Production written by Patrick C. Hallenbeck and published by Springer Science & Business Media. This book was released on 2011-12-16 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concerns over dwindling fossil fuel reserves and impending climate changes have focused attention worldwide on the need to discover alternative, sustainable energy sources and fuels. Biofuels, already produced on a massive industrial scale, are seen as one answer to these problems. However, very real concerns over the effects of biofuel production on food supplies, with some of ht recent increases in worldwide food costs attributable to biofuel production, have lead to the realization that new, non-food substrates for biofuel production must be bought online. This book is an authoritative, comprehensive, up-to-date review of the various options under development for the production of advanced biofuels as alternative energy sources. A general overview and introductory chapters for each section place the field in the context as well as provide essential basic notions for the more general reader. Accomplished, internationally recognized experts carrying out research on individual focus areas contribute specific technical chapters detailing present progress and future prospects.
Download or read book Bioeconomy written by Iris Lewandowski and published by Springer. This book was released on 2017-12-11 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is open access under a CC BY 4.0 license. This book defines the new field of "Bioeconomy" as the sustainable and innovative use of biomass and biological knowledge to provide food, feed, industrial products, bioenergy and ecological services. The chapters highlight the importance of bioeconomy-related concepts in public, scientific, and political discourse. Using an interdisciplinary approach, the authors outline the dimensions of the bioeconomy as a means of achieving sustainability. The authors are ideally situated to elaborate on the diverse aspects of the bioeconomy. They have acquired in-depth experience of interdisciplinary research through the university’s focus on “Bioeconomy”, its contribution to the Bioeconomy Research Program of the federal state of Baden-Württemberg, and its participation in the German Bioeconomy Council. With the number of bioeconomy-related projects at European universities rising, this book will provide graduate students and researchers with background information on the bioeconomy. It will familiarize scientific readers with bioeconomy-related terms and give scientific background for economists, agronomists and natural scientists alike.
Book Synopsis Renewable Resources for Biorefineries by : Carol Lin
Download or read book Renewable Resources for Biorefineries written by Carol Lin and published by Royal Society of Chemistry. This book was released on 2014-08-11 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the increasing awareness and concern about the dependency on fossil resources and the depletion of crude oil reserves, experts from industrial biotechnology, renewable resources, green chemistry, and biorefineries are stimulating the transition from the fossil-based to the bio-based economy. This text confronts scientific and economic challenges and strategies for making this crucial transition. Renewable Resources for Biorefineries is the work of a strongly interdisciplinary authorship, offering perspectives from biology, chemistry, biochemical engineering, materials science, and industry. This unique approach provides an opportunity for a much broader coverage of biomass and valorisation than has been attempted in previous titles. This book also represents the fundamentally important technical and policy aspects of a bio-based economy, to ground this important science in a realistic and viable economic framework. Chapters in this book cover a diverse range of topics, including: advanced generation bioenergy sectors; biobased polymers and materials; chemical platform molecules; industrial crops and biorefineries; financing and policy for change; and valorisation of biomass waste streams. This is an ideal book for upper level undergraduate and postgraduate students taking modules on Renewable resources, green chemistry, sustainable development, environmental science, agricultural science and environmental technology. It will also benefit industry professionals and product developers who are looking to improve economic and environmental ways to utilise renewable resources in current and future biorefineries.
Book Synopsis Advances of Basic Science for Second Generation Bioethanol from Sugarcane by : Marcos S. Buckeridge
Download or read book Advances of Basic Science for Second Generation Bioethanol from Sugarcane written by Marcos S. Buckeridge and published by Springer. This book was released on 2017-03-10 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the basic science recently produced in Brazil for the improvement of sugarcane as a bioenergy crop and as a raw material for 2nd generation bioethanol production. It reports achievements that have been advancing the science of cell walls, enzymes, genetics, and sustainability related to sugarcane technologies and give continuity to the research reported in the “Routes to Cellulosic Ethanol”, from Springer. The Introduction (Chapter I) explains how the National Institute of Science and Technology of Bioethanol, founded in 2008 in Brazil, became part of the main international initiatives that started to search for forms to use biomass for bioethanol production in Brazil, US and Europe. Part I reports the advances in plant cell wall composition, structure and architecture, and physical characteristics of sugarcane biomass. These discoveries are opening the way to increased efficiency of pretreatments and hydrolysis, being therefore important information for 2nd generation processes as well as for biorefinery initiatives. Part II focuses on the discovery and characterization of hydrolases from microorganisms that could be used in industrial processes. Recent advances in the search for hydrolases using metagenomics is reported. A great number of genes and enzymes from microorganisms have been discovered, affording improvement of enzyme cocktails better adapted to sugarcane biomass. Part III reports two key issues in the process of 2G ethanol, pentose fermentation and sugarcane genetics. These are the discoveries of new yeast species capable of producing ethanol more efficiently from xylose and the advances made on the sugarcane genetics, a key issue to design varieties adapted to 2G ethanol production. Part IV approaches sustainability through two chapters, one discussing the sustainability of the sugarcane agricultural and environmental system and another discussing how national and mainly international policies of Brazil regarding 2G ethanol production affected the country’s strategies to establish itself as an international player in renewable energy area.
