Bacterial Artificial Chromosomes

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Bacterial Artificial Chromosomes

Author : Shaying Zhao
Publisher : Springer Science & Business Media
ISBN 13 : 159259753X
Total Pages : 333 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Bacterial Artificial Chromosomes by : Shaying Zhao

Download or read book Bacterial Artificial Chromosomes written by Shaying Zhao and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several developmental and historical threads are woven and displayed in these two volumes of Bacterial Artificial Chromosomes, the first on Library Construction, Physical Mapping, and Sequencing, and the second on Fu- tional Studies. The use of large-insert clone libraries is the unifying feature, with many diverse contributions. The editors have had quite distinct roles. Shaying Zhao has managed several BAC end-sequencing projects. Marvin Stodolsky during 1970–1980 contributed to the elucidation of the natural b- teriophage/prophage P1 vector system. Later, he became a member of the Genome Task Group of the Department of Energy (DOE), through which s- port flowed for most clone library resources of the Human Genome Program (HGP). Some important historical contributions are not represented in this volume. This preface in part serves to mention these contributions and also briefly surveys historical developments. Leon Rosner (deceased) contributed substantially in developing a PAC library for drosophila that utilized a PI virion-based encapsidation and tra- fection process. This library served prominently in the Drosophila Genome Project collaboration. PACs proved easy to purify so that they substantially replaced the YACs used earlier. Much of the early automation for massive clone picking and processing was developed at the collaborating Lawrence Berkeley National Laboratory. However, the P1 virion encapsidation system itself was too fastidious, and P1 virion-based methods did not gain popularity in other genome projects.

Bacterial Artificial Chromosomes

Author : Pradeep Chatterjee
Publisher : BoD – Books on Demand
ISBN 13 : 9533077255
Total Pages : 152 pages
Book Rating : 4.5/5 (33 download)

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Book Synopsis Bacterial Artificial Chromosomes by : Pradeep Chatterjee

Download or read book Bacterial Artificial Chromosomes written by Pradeep Chatterjee and published by BoD – Books on Demand. This book was released on 2011-11-25 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the numerous applications of Bacterial Artificial Chromosomes (BACs) in a variety of studies. The topics reviewed range from using BAC libraries as resources for marsupial and monotreme gene mapping and comparative genomic studies, to using BACs as vehicles for maintaining the large infectious DNA genomes of viruses. The large size of the insert DNA in BACs and the ease of engineering mutations in that DNA within the bacterial host, allowed manipulating the BAC-viral DNA of Varicella-Zoster Virus. Other reviews include the maintenance and suitable expression of foreign genes from a Baculovirus genome, including protein complexes, from the BAC-viral DNA and generating vaccines from BAC-viral DNA genomes of Marek's disease virus. Production of multi-purpose BAC clones in the novel Bacillus subtilis host is described, along with chapters that illustrate the use of BAC transgenic animals to address important issues of gene regulation in vertebrates, such as functionally identifying novel cis-acting distal gene regulatory sequences.

Bacterial Artificial Chromosomes

Author : Shaying Zhao
Publisher : Springer Science & Business Media
ISBN 13 : 1592597521
Total Pages : 367 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Bacterial Artificial Chromosomes by : Shaying Zhao

Download or read book Bacterial Artificial Chromosomes written by Shaying Zhao and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: For both volumes: Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Bacterial Artificial Chromosomes

Author : Pradeep Chatterjee
Publisher :
ISBN 13 : 9789535151753
Total Pages : 150 pages
Book Rating : 4.1/5 (517 download)

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Book Synopsis Bacterial Artificial Chromosomes by : Pradeep Chatterjee

Download or read book Bacterial Artificial Chromosomes written by Pradeep Chatterjee and published by . This book was released on 2011 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the numerous applications of Bacterial Artificial Chromosomes (BACs) in a variety of studies. The topics reviewed range from using BAC libraries as resources for marsupial and monotreme gene mapping and comparative genomic studies, to using BACs as vehicles for maintaining the large infectious DNA genomes of viruses. The large size of the insert DNA in BACs and the ease of engineering mutations in that DNA within the bacterial host, allowed manipulating the BAC-viral DNA of Varicella-Zoster Virus. Other reviews include the maintenance and suitable expression of foreign genes from a Baculovirus genome, including protein complexes, from the BAC-viral DNA and generating vaccines from BAC-viral DNA genomes of Marek's disease virus. Production of multi-purpose BAC clones in the novel Bacillus subtilis host is described, along with chapters that illustrate the use of BAC transgenic animals to address important issues of gene regulation in vertebrates, such as functionally identifying novel cis-acting distal gene regulatory sequences.

Mammalian Artificial Chromosomes

Author : Vittorio Sgaramella
Publisher : Springer Science & Business Media
ISBN 13 : 1592594344
Total Pages : 277 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Mammalian Artificial Chromosomes by : Vittorio Sgaramella

Download or read book Mammalian Artificial Chromosomes written by Vittorio Sgaramella and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1996, we organized a workshop, inter alia, at the National Research Co- cil in Milan under the generous sponsorship of the European Science Foun- tion. On that occasion, a small group of investigators convened from many countries and presented early evidence of the possibility of assembling basic units of mammalian chromosomes into artificial constructs (or, indeed, red- ing the relevant components to more manageable dimensions and defined c- stitution). Progress in the following years has been slow but steady. Many scientists who took part in the workshop have since been engaged in active and prod- tive research. It goes to the credit of Humana Press to have realized the need for a book on artificial chromosomes that aims to provide better tools to all scientists committed to this field who are confronted with very difficult tech- cal problems. We have strived to cover in Mammalian Artificial Chromosomes: Methods and Protocols all relevant areas of artificial chromosome research, from basic genetics to daring attempts to build new tools for genetic therapy. We are of course grateful to the authors who have accepted the task of describing the technical steps and pitfalls that can be encountered in their research. Rarely has a very delicate methodology been presented with such meticulous care. We have been helped in this enterprise by the excellent librarian of the LITA Institute in Segrate, Italy, Ms. Claudia Piergigli, whom we thank warmly. Ms.

Bacterial Chromosomes—Advances in Research and Application: 2012 Edition

Author :
Publisher : ScholarlyEditions
ISBN 13 : 1481632973
Total Pages : 23 pages
Book Rating : 4.4/5 (816 download)

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Book Synopsis Bacterial Chromosomes—Advances in Research and Application: 2012 Edition by :

Download or read book Bacterial Chromosomes—Advances in Research and Application: 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 23 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial Chromosomes—Advances in Research and Application: 2012 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Bacterial Chromosomes in a compact format. The editors have built Bacterial Chromosomes—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Bacterial Chromosomes in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Bacterial Chromosomes—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Human Bacterial Artificial Chromosome (BAC) Clones

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (442 download)

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Book Synopsis Human Bacterial Artificial Chromosome (BAC) Clones by :

Download or read book Human Bacterial Artificial Chromosome (BAC) Clones written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers access to tools to search the the Institute for Genomic Research (TIGR) Bacterial Artificial Chromosome (BAC) Database, based in Rockville, Maryland. Explains the significance of sequences from human bacterial artificial chromosome (BAC) clones.

Development of an Akata-based Bacterial Artificial Chromosome

Author : Lavon Paongo Tonga
Publisher :
ISBN 13 : 9781109829891
Total Pages : 104 pages
Book Rating : 4.8/5 (298 download)

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Book Synopsis Development of an Akata-based Bacterial Artificial Chromosome by : Lavon Paongo Tonga

Download or read book Development of an Akata-based Bacterial Artificial Chromosome written by Lavon Paongo Tonga and published by . This book was released on 2006 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic mutagenesis of individual genes in context of the complete Epstein-Barr virus genome is essential to understanding function of proteins. Past strategies for deriving a mutant within EBV have been to disrupt a gene with a cassette encoding for antibiotic resistance flanked with sequence surrounding the targeted gene. The cassette is transfected into virus-carrying cells and positive selection is applied to select for a genome in which homologous recombination with flanking sequence has occurred. Although this method has met with success, it has limitations. It is time consuming, since a successful insertion of the cassette relies on a rare recombination event in the eukaryotic cell, and it allows only a narrow range of mutations to be made. Several herpesviruses have been cloned as bacterial artificial chromosomes (BAC) where a mini-F' plasmid is cloned into the viral genome so that it can be propagated in both eukaryotic and prokaryotic cells. Standard bacterial genetics can then be applied to the whole viral genome in prokaryotic cells prior to transferring it into eukaryotic cells for production of mutant progeny.

Plant Molecular Biology Manual

Author : Stanton Gelvin
Publisher : Springer Science & Business Media
ISBN 13 : 940115242X
Total Pages : 138 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Plant Molecular Biology Manual by : Stanton Gelvin

Download or read book Plant Molecular Biology Manual written by Stanton Gelvin and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Life Sciences

Author : Robert D. Wells
Publisher : Springer
ISBN 13 : 9781461415299
Total Pages : 0 pages
Book Rating : 4.4/5 (152 download)

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Book Synopsis Molecular Life Sciences by : Robert D. Wells

Download or read book Molecular Life Sciences written by Robert D. Wells and published by Springer. This book was released on 2018-01-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Life Sciences: An Encyclopedic Reference will focus on understanding biological phenomena at the level of molecules and their interactions that govern life processes. The work will include articles on genes and genomes, protein structure and function, systems biology using genomics and proteomics as the focus, molecular aspects of cell structure and function, unifying concepts and theories from biology, chemistry, mathematics and physics that are essential for understanding the molecular life sciences (including teaching perspectives and assessment tools), and basic aspects of the various experimental approaches that are used in the Molecular Life Sciences.

Echinoderms Part B

Author :
Publisher : Academic Press
ISBN 13 : 0128170735
Total Pages : 552 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Echinoderms Part B by :

Download or read book Echinoderms Part B written by and published by Academic Press. This book was released on 2019-04-02 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: Echinoderms, Volume 151, the latest release in the Methods in Cell Biology series, highlights advances in the field, with this update presenting chapters on Echinoderm Genome Databases, analysis of gene regulatory networks, using ATAC-seq and RNA-seq to increase resolution in GRN connectivity, multiplex cis-regulatory analysis, experimental approaches GRN/signal pathways, BACs, analysis of chromatin accessibility using ATAC-seq, analysis of sea urchin proteins /Click IT, CRISPR/Cas9-mediated genome editing in sea urchins, super-resolution and in toto imaging of echinoderm embryos, and methods for analysis of intracellular ion signals in sperm, eggs and embryos. - Presents clear, concise protocols provided by experts who have established the echinoderms as a model systems - Highlights new advances in the field, with this update presenting interesting chapters on echinoderms

The Screening of a Horse Bacterial Artificial Chromosome (BAC) Library with Type I Markers and the Isolation and Characterization of Microsatellites

Download The Screening of a Horse Bacterial Artificial Chromosome (BAC) Library with Type I Markers and the Isolation and Characterization of Microsatellites PDF Online Free

Author : Elaine Lynnette Philbrick
Publisher :
ISBN 13 :
Total Pages : 164 pages
Book Rating : 4.:/5 (66 download)

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Book Synopsis The Screening of a Horse Bacterial Artificial Chromosome (BAC) Library with Type I Markers and the Isolation and Characterization of Microsatellites by : Elaine Lynnette Philbrick

Download or read book The Screening of a Horse Bacterial Artificial Chromosome (BAC) Library with Type I Markers and the Isolation and Characterization of Microsatellites written by Elaine Lynnette Philbrick and published by . This book was released on 2002 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Human Chromosomes

Author : Orlando J. Miller
Publisher : Springer Science & Business Media
ISBN 13 : 1461301394
Total Pages : 508 pages
Book Rating : 4.4/5 (613 download)

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Book Synopsis Human Chromosomes by : Orlando J. Miller

Download or read book Human Chromosomes written by Orlando J. Miller and published by Springer Science & Business Media. This book was released on 2011-06-28 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of this well-known text provides students, researchers and technicians in the area of medicine, genetics and cell biology with a concise, understandable introduction to the structure and behavior of human chromosomes. This new edition continues to cover both basic and up-to-date material on normal and defective chromosomes, yet is particularly strengthened by the complete revision of the material on the molecular genetics of chromosomes and chromosomal defects. The mapping and molecular analysis of chromosomes is one of the most exciting and active areas of modern biomedical research, and this book will be invaluable to scientists, students, technicians and physicians with an interest in the function and dysfunction of chromosomes.

Structure and Function of the Bacterial Genome

Author : Charles J. Dorman
Publisher : John Wiley & Sons
ISBN 13 : 1119308798
Total Pages : 437 pages
Book Rating : 4.1/5 (193 download)

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Book Synopsis Structure and Function of the Bacterial Genome by : Charles J. Dorman

Download or read book Structure and Function of the Bacterial Genome written by Charles J. Dorman and published by John Wiley & Sons. This book was released on 2020-04-28 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents an integrated view of the expression of bacterial genetic information, genome architecture and function, and bacterial physiology and pathogenesis This book blends information from the very latest research on bacterial chromosome and nucleoid architecture, whole-genome analysis, cell signaling, and gene expression control with well-known gene regulation paradigms from model organisms (including pathogens) to give readers a picture of how information flows from the environment to the gene, modulating its expression and influencing the competitive fitness of the microbe. Structure and Function of the Bacterial Genome explores the governance of the expression of the genes that make a bacterium what it is, and updates the basics of gene expression control with information about transcription promoter structure and function, the role of DNA as a regulatory factor (in addition to its role as a carrier of genetic information), small RNAs, RNAs that sense chemical signals, ribosomes and translation, posttranslational modification of proteins, and protein secretion. It looks at the forces driving the conservation and the evolution of the dynamic genome and offers chapters that cover DNA replication, DNA repair, plasmid biology, recombination, transposition, the roles of repetitive DNA sequences, horizontal gene transfer, the defense of the genome by CRISPR-Cas, restriction enzymes, Argonaute proteins and BREX systems. The book finishes with a chapter that gives an integrated overview of genome structure and function. Blends knowledge of gene regulatory mechanisms with a consideration of nucleoid structure and dynamics Offers a 'DNA-centric' approach to considering transcription control Views horizontal gene transfer from a gene regulation perspective Assesses the opportunities and limitations of designing synthetic microbes or rewiring existing ones Structure and Function of the Bacterial Genome is an ideal book for graduate and undergraduate students studying microbial cell biology, bacterial pathogenesis, gene regulation, and molecular microbiology. It will also appeal to principal investigators conducting research on these and related topics and researchers in synthetic biology and other arms of biotechnology.

E. coli Plasmid Vectors

Author : Nicola Casali
Publisher : Humana Press
ISBN 13 : 9781617373916
Total Pages : 316 pages
Book Rating : 4.3/5 (739 download)

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Book Synopsis E. coli Plasmid Vectors by : Nicola Casali

Download or read book E. coli Plasmid Vectors written by Nicola Casali and published by Humana Press. This book was released on 2010-11-10 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

The Potato Genome

Author : Swarup Kumar Chakrabarti
Publisher : Springer
ISBN 13 : 3319661353
Total Pages : 332 pages
Book Rating : 4.3/5 (196 download)

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Book Synopsis The Potato Genome by : Swarup Kumar Chakrabarti

Download or read book The Potato Genome written by Swarup Kumar Chakrabarti and published by Springer. This book was released on 2017-12-26 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the historical importance of potato (Solanum tuberosum L.),potato genetic resources and stocks (including S. tuberosum group Phureja DM1-3 516 R44, a unique doubled monoploid homozygous line) used for potato genome sequencing. It also discusses strategies and tools for high-throughput sequencing, sequence assembly, annotation, analysis, repetitive sequences and genotyping-by-sequencing approaches. Potato (Solanum tuberosum L.; 2n = 4x = 48) is the fourth most important food crop of the world after rice, wheat and maize and holds great potential to ensure both food and nutritional security. It is an autotetraploid crop with complex genetics, acute inbreeding depression and a highly heterozygous nature. Further, the book examines the recent discovery of whole genome sequencing of a few wild potato species genomes, genomics in management and genetic enhancement of Solanum species, new strategies towards durable potato late blight resistance, structural analysis of resistance genes, genomics resources for abiotic stress management, as well as somatic cell genetics and modern approaches in true-potato-seed technology. The complete genome sequence provides a better understanding of potato biology, underpinning evolutionary process, genetics, breeding and molecular efforts to improve various important traits involved in potato growth and development.

Molecular Biology and Genomics

Author : Cornel Mulhardt
Publisher : Elsevier
ISBN 13 : 0080467660
Total Pages : 273 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Molecular Biology and Genomics by : Cornel Mulhardt

Download or read book Molecular Biology and Genomics written by Cornel Mulhardt and published by Elsevier. This book was released on 2010-07-19 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: Never before has it been so critical for lab workers to possess the proper tools and methodologies necessary to determine the structure, function, and expression of the corresponding proteins encoded in the genome. Mulhardt's Molecular Biology and Genomics helps aid in this daunting task by providing the reader with tips and tricks for more successful lab experiments. This strategic lab guide explores the current methodological variety of molecular biology and genomics in a simple manner, addressing the assets and drawbacks as well as critical points. It also provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. - Shows how to avoid experimental dead ends and develops an instinct for the right experiment at the right time - Includes a handy Career Guide for researchers in the field - Contains more than 100 extensive figures and tables