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Atomic Force Microscopy And Force Spectroscopy Of Biomembranes And Applications To Nanotechnology
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Book Synopsis Atomic Force Microscopy and Force Spectroscopy of Biomembranes and Applications to Nanotechnology by : Kislon Voitchovsky
Download or read book Atomic Force Microscopy and Force Spectroscopy of Biomembranes and Applications to Nanotechnology written by Kislon Voitchovsky and published by . This book was released on 2006 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Manipulation with Atomic Force Microscopy by : Anne-Sophie Duwez
Download or read book Molecular Manipulation with Atomic Force Microscopy written by Anne-Sophie Duwez and published by CRC Press. This book was released on 2011-12-07 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the invention of scanning probe techniques in the early 1980s, scientists can now play with single atoms, single molecules, and even single bonds. Force, dynamics, and function can now be probed at the single-molecule level. Molecular Manipulation with Atomic Force Microscopy (AFM) presents a series of topics that discuss concepts and methodologies used to manipulate and study single (bio)molecules with AFM. The first part is dedicated to the pulling of single molecules with force spectroscopy to investigate molecular interactions, mechanics, and mechanochemical processes, and the second part to the manipulation, repositioning, and targeted delivery of single molecules on substrates. Single molecule manipulation is an exciting area of research which made important breakthroughs in nanoscience and which could find potential applications in a diverse range of disciplines, including chemistry, biology, physics, material and polymer science, and engineering. New and experienced AFM researchers looking for applications beyond imaging will find a wealth of information in this informative volume.
Book Synopsis Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobotics by : Mi Li
Download or read book Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobotics written by Mi Li and published by Springer. This book was released on 2017-10-06 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents methodological and application research in detecting cellular and molecular biophysical properties based on atomic force microscopy (AFM) nanorobotics. Series methods for in situ label-free visualizing and quantifying the multiple physical properties of single cells and single molecules were developed, including immobilization strategies for observing fine structures of living cells, measurements of single-cell mechanics, force recognition of molecular interactions, and mapping protein organizations on cell surface. The biomedical applications of these methods in clinical lymphoma treatments were explored in detail, including primary sample preparation, cancer cell recognition, AFM detection and data analysis. Future directions about the biomedical applications of AFM are also given.
Book Synopsis Quantitative Measurements of Nano Forces Using Atomic Force Microscopy (AFM) : Quantifying Nano Forces in Three-dimensions Using AFM : Applications in the Biological, Physical and Chemical Sciences by : Gregory Shaun Watson
Download or read book Quantitative Measurements of Nano Forces Using Atomic Force Microscopy (AFM) : Quantifying Nano Forces in Three-dimensions Using AFM : Applications in the Biological, Physical and Chemical Sciences written by Gregory Shaun Watson and published by . This book was released on 2008 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Dynamic Force Spectroscopy and Biomolecular Recognition by : Anna Rita Bizzarri
Download or read book Dynamic Force Spectroscopy and Biomolecular Recognition written by Anna Rita Bizzarri and published by CRC Press. This book was released on 2012-01-25 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular recognition, also known as biorecognition, is the heart of all biological interactions. Originating from protein stretching experiments, dynamic force spectroscopy (DFS) allows for the extraction of detailed information on the unbinding process of biomolecular complexes. It is becoming progressively more important in biochemical studies a
Book Synopsis New Techniques for Studying Biomembranes by : Qiu-Xing Jiang
Download or read book New Techniques for Studying Biomembranes written by Qiu-Xing Jiang and published by CRC Press. This book was released on 2020-03-18 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Techniques for Studying Biomembranes describes some of the latest methods used to investigate the dynamic distribution of specific lipids in membranes and their effects on other membrane components. The contributors present important discoveries with respect to lipid analysis and lipid interactions with membrane proteins. Various methods, which have been used to study lipid bilayer structure and lipid organization in membranes, include both in vitro and in vivo membrane systems, and study membrane proteins in various membrane systems. Key Features: Reviews both in vivo and in vitro analytical technologies and methods for studying membrane structure and function Explores how lipid bilayers and membrane proteins interact Includes contributions from an international team of researchers actively studying membrane structure and function Identifies various diseases whose causes are related to membrane proteins Related Titles: Christopher R. Jacobs, Hayden Huang, and Ronald Y. Kwon. Introduction to Cell Mechanics and Mechanobiology (ISBN 978-0-8153-4425-4) Wendell Lim and Bruce Mayer. Cell Signaling: Principles and Mechanisms (ISBN 978-0-8153-4244-1) Stephen Rothman. Proteins Crossing Membranes: A Scientist’s Memoir (978-0-3670-7449-4)
Book Synopsis Force Spectroscopy to Understand Cell Surface Receptor Interactions in the Immune System by : Joost Riet
Download or read book Force Spectroscopy to Understand Cell Surface Receptor Interactions in the Immune System written by Joost Riet and published by . This book was released on 2010 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the invention of the atomic force microscope (AFM) in 1986, the application of the instrument has shifted from a pure physicist’s tool to a useful instrument for physicists, chemists and biologists. The AFM is not a classical microscope in the sense that light or electrons are used to obtain an image, it is rather a mechanical microscope. The imaging is purely based on a physical probing of the surface topography by raster scanning (zigzag movements) of a sharp tip on a cantilever across the sample. Furthermore, the AFM can probe forces with ultra-high sensitivity by moving the cantilever in the vertical direction, which is especially interesting for biological applications. The probing of forces with AFM is known as AFM force spectroscopy. These AFM measurements can be performed under physiological conditions and make it possible to study dynamical processes, such as the adhesion of a living cell to an adjacent cell or the extracellular matrix, from the single cell to single molecular level. In the immune system diverse dynamical processes, such as the recognition of a pathogen by a dendritic cell (DC) or the transmigration of a leukocyte through the endothelium of a blood vessel, happen all the time throughout the human body. In the latter case during initial binding of these cells, bonds between different cell adhesion molecules (CAMs) are formed. These interactions have to be highly dynamic to withstand forces induced by for example the blood flow. These CAM bonds associate and dissociate at rates that change considerably under conditions of cell stress. The combination of molecular cell biology and AFM single molecule force spectroscopy provides a powerful tool to explore the complexity of these cell adhesion processes. In this thesis, we explored the versatility of the AFM in the context of biomaterial science, nano-chemistry and cell biology. The high spatial resolution of the AFM was exploited to measure and manipulate samples at the nanoscale. Moreover, the high force sensitivity of the AFM was applied to measure adhesive properties of CAMs on cells and to address the two distinct adhesive regulation processes of a CAM: affinity and avidity.
Book Synopsis Fluorescence Imaging and Biological Quantification by : Raquel Seruca
Download or read book Fluorescence Imaging and Biological Quantification written by Raquel Seruca and published by CRC Press. This book was released on 2017-09-06 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.
Book Synopsis Handbook of Single-Molecule Biophysics by : Peter Hinterdorfer
Download or read book Handbook of Single-Molecule Biophysics written by Peter Hinterdorfer and published by Springer Science & Business Media. This book was released on 2009-12-24 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Book Synopsis An Introduction to Single Molecule Biophysics by : Yuri L. Lyubchenko
Download or read book An Introduction to Single Molecule Biophysics written by Yuri L. Lyubchenko and published by CRC Press. This book was released on 2017-11-22 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.
Book Synopsis Studying Biomolecular Structures and Their Interaction Using Atomic Force Microscopy by : Subhadip Senapati
Download or read book Studying Biomolecular Structures and Their Interaction Using Atomic Force Microscopy written by Subhadip Senapati and published by . This book was released on 2015 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopy (AFM) has become an important tool to characterize and image surfaces with nanoscale resolution. AFM imaging technique has been utilized to study a wide range of substances such as DNA, proteins, cells, silicon surfaces, nanowires etc. Hence AFM has become extremely important in the field of biochemistry, cell biology and material science. Functionalizing the AFM tip made it possible to detect molecules and their interaction using recognition imaging at single molecule level. Also the unbinding force of two molecules can be investigated based on AFM based single molecule force spectroscopy. In the first study, a new chemical approach to functionalize the AFM tip in a simple and user-friendly way has been described. Copper-free click chemistry and a vinyl sulfone PEG linker have been utilized during the process. Using this technique, human thrombin and integrin were detected in separate experiments. Then a novel tri-arm linker with two recognition molecules on it was designed and two proteins (human thrombin and integrin) were detected simultaneously in the same experiment using recognition imaging. This technique can be applied to understand many multivalent interactions taking place in nature. Using the same tri-arm linker functionalized with two biotin molecules, the interaction of streptavidin with mono-biotin and bis-biotin ligands were investigated. The thermal stability of streptavidin-biotin complex was also studied using SDS-PAGE analysis. In the final study, structure of native chromatin extracted from normal and cancer cell lines were analyzed using AFM imaging and agarose gel electrophoresis. Different salt fractions were used to extract chromatin region depending on their solubility. Mnase sensitivity of the chromatin sample was used to understand the open and closed structures of chromatin from different sources. The amount of chromatin in different salt fractions could act as an indicator of amount of open and condensed chromatin in normal and cancer cells. Eventually this ratio of closed and open structure of chromatin could be an indicator of tumorigenic nature of particular cell lines.
Book Synopsis Imaging and Manipulation of Adsorbates Using Dynamic Force Microscopy by : Philip Moriarty
Download or read book Imaging and Manipulation of Adsorbates Using Dynamic Force Microscopy written by Philip Moriarty and published by Springer. This book was released on 2015-04-28 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: Imaging and Manipulation of Adsorbates using Dynamic Force Microscopy provides an overview of the latest developments in dynamic force microscopy (DFM) of atoms, molecules, and nanoparticles adsorbed on solid surfaces. Significant advances in the capabilities of this technique have been made in the last decade and this book represents a timely snapshot of the major research themes in the field, with a particular focus on the manipulation of matter at the atomic and (sub)molecular levels. This edited volume will be of keen interest to researchers active in nanoscience and its various sub-fields including, in particular, scanning probe microscopy. This book expands on the previous volumes in the series Advances in Atom and Single Molecule Machines. DFM is an exceptionally powerful tool for the imaging and probing of adsorbates on insulators and is now a component of the type of multiprobe interconnection systems described in Vol. 1 of the series. DFM can also be used to translate atoms and molecules in the context of the fabrication of the type of logic gates described in Vol. 2. When used in conjunction with STM, DFM also enables a detailed comparison of the chemical ‘architecture’ of a molecule with the spatial distribution of its orbital density, as described in Vol. 3. In this book readers will gain key insights into the current capabilities, and future potential, of dynamic force microscopy.
Book Synopsis Nanoscale Investigations of Biomaterials Using Atomic Force Microscopy and Enhanced Raman Spectroscopy by : Xiaobin Yao
Download or read book Nanoscale Investigations of Biomaterials Using Atomic Force Microscopy and Enhanced Raman Spectroscopy written by Xiaobin Yao and published by . This book was released on 2021* with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In this thesis, AFM and enhanced Raman spectroscopy were applied for investigating the mode of action of CaL and the detection of SARS-CoV-2. Last but not least, a simple method for suppressing sample degradation in enhanced Raman spectroscopy will be introduced. Chapter 1 introduces some representative super-resolution techniques, including near-field scanning optical microscopy, far-field super-resolution fluorescent microscopy, and non-optical microscopy. Among these techniques, atomic force microscopy and Raman spectroscopy are highlighted. The principle of atomic force microscopy in topographical and mechanical research is explained in Chapter 1.2. Raman spectroscopy, including conventional Raman, SERS, and TERS are specified in Chapter 1.3. A short outline of this thesis is listed in Chapter 1.4. All instruments and experimental materials used in the experiments are discussed in Chapter 2. Investigations of water-resistant silver (Ag) SERS substrates are presented in Chapter 3. More importantly, a simple and very promising method, which suppresses sample degradation in enhanced Raman spectroscopy, will be firstly introduced in this chapter. This method furthermore improves the reliability and reproducibility of measurements using enhanced Raman spectroscopy. Chapter 4 is concerned with the applications of AFM and enhanced Raman spectroscopy in the investigations of the fungal peptide CaL. The mode of action of CaL, especially its pore formation, is investigated and discussed using AFM and Raman spectroscopy for the first time. The same AFM-TERS setup was also used to investigate SARS-CoV-2. Topographical, mechanical, and chemical information of SARS-CoV-2 virions are presented in Chapter 5. It is found that with the combination of topographical and mechanical information, AFM was able to recognize virions. TERS was preliminary applied to the analysis of the surface chemical compositions of virus-like particles.
Book Synopsis Applications of Electrochemistry and Nanotechnology in Biology and Medicine II by : Noam Eliaz
Download or read book Applications of Electrochemistry and Nanotechnology in Biology and Medicine II written by Noam Eliaz and published by Springer Science & Business Media. This book was released on 2011-12-03 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of electrochemical nanotechnology has emerged as researchers apply electrochemistry to nanoscience and nanotechnology. These two related volumes in the Modern Aspects of Electrochemistry Series review recent developments and breakthroughs in the specific application of electrochemistry and nanotechnology to biology and medicine. Internationally renowned experts contribute chapters that address both fundamental and practical aspects of several key emerging technologies in biomedicine, such as the processing of new biomaterials, biofunctionalization of surfaces, characterization of biomaterials, discovery of novel phenomena and biological processes occurring at the molecular level.
Book Synopsis Biomimetic Lipid Membranes: Fundamentals, Applications, and Commercialization by : Fatma N. Kök
Download or read book Biomimetic Lipid Membranes: Fundamentals, Applications, and Commercialization written by Fatma N. Kök and published by Springer. This book was released on 2019-04-16 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book compiles the fundamentals, applications and viable product strategies of biomimetic lipid membranes into a single, comprehensive source. It broadens its perspective to interdisciplinary realms incorporating medicine, biology, physics, chemistry, materials science, as well as engineering and pharmacy at large. The book guides readers from membrane structure and models to biophysical chemistry and functionalization of membrane surfaces. It then takes the reader through a myriad of surface-sensitive techniques before delving into cutting-edge applications that could help inspire new research directions. With more than half the world's drugs and various toxins targeting these crucial structures, the book addresses a topic of major importance in the field of medicine, particularly biosensor design, diagnostic tool development, vaccine formulation, micro/nano-array systems, and drug screening/development. Provides fundamental knowledge on biomimetic lipid membranes; Addresses some of biomimetic membrane types, preparation methods, properties and characterization techniques; Explains state-of-art technological developments that incorporate microfluidic systems, array technologies, lab-on-a-chip-tools, biosensing, and bioprinting techniques; Describes the integration of biomimetic membranes with current top-notch tools and platforms; Examines applications in medicine, pharmaceutical industry, and environmental monitoring.
Book Synopsis Nano-Scale Characterization of Biomolecules Using Atomic Force Microscopy by : Jung reem Woo
Download or read book Nano-Scale Characterization of Biomolecules Using Atomic Force Microscopy written by Jung reem Woo and published by . This book was released on 2015 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopy (AFM) is a class of high-resolution scanning probe microscopy (SPM) for non-conducting materials. Of these non-conducting materials, biomolecules such as cells, organelles, proteins, and nucleic acids are of interest as we seek to describe laws of nature and life. However, it is not trivial to resolve structures or mechanics of biomolecules due to their small sizes and soft constructions. AFM enables one to obtain high-resolution images of biological structures in their native state both in air and in fluids as AFM is considered as a non-destructive technique. In addition to these advantages, AFM is providing the mechanical information of the sample surface. For these reasons, since the development of AFM, an expansion and re-assessment has led to significant discoveries and scientific enlightenment in biology. In this dissertation, I focus on the application of AFM to biological systems, which will add new scores in the field of both microscopy as well as a higher understanding of specific biological systems. I have explored the mechanical properties of mammalian cells and bacterial cells. I have examined mammalian cell stiffness in relation to cancer metastasis and the changes in extracellular biofilm adhesions from bacterial cells. I have resolved the high-resolution structure of exosomes which are 50-120 nm sized vesicles secreted from mammalian cells. With an accurate adjustment of the force applied to the sample, I have been able to observe and verify nanofilaments attached to exosomes. I also have discovered that the surface and the size distributions of exosomes are different depending on their purification methods. Lastly, I have obtained high-resolution images of an actin binding protein, INF2, which showed nanostructured self-assemblies with or without f-actin.
Book Synopsis Studying Biomolecular Interactions Using Atomic Force Microscopy by : Carolin Madwar
Download or read book Studying Biomolecular Interactions Using Atomic Force Microscopy written by Carolin Madwar and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many biological systems involve mechanical interactions and specific molecular recognition events that are essential for their function. Single molecule force spectroscopy (SMFS) is a powerful and versatile atomic force microscopy (AFM)-based technique, which allows probing such interactions at the single molecule level, with piconewton sensitivity, thereby illuminating their dynamics as well as their structural and mechanical properties. A fundamental requirement in these studies is the immobilization of biomolecules between the AFM probe and the sample surface, preferably by a long flexible molecular spacer, such as poly(ethylene glycol) (PEG). The goal of this project is to investigate the binding interactions in four distinct biomolecular systems at the single molecule level, using SMFS. A new amination strategy was used to attach a novel bifunctional PEG spacer containing amine- and thiol-reactive termini. Biomolecules under investigation were tethered to AFM tips by formation of covalent linkages to this versatile spacer. Various tests, including surface plasmon resonance and a UV-based enzyme assay, were carried out to evaluate and confirm AFM tip functionalization. SMFS of biotin/streptavidin yielded an average unbinding force of 59 pN at 4000 pN/s loading rate. SMFS of concanavalin A/mannose yielded an average unbinding force of 50 pN at loading rate of 6000 pN/s. SMFS of a de novo heterodimeric E/K coiled/coil yielded an average unbinding force of 41 pN at 7000 pN/s loading rate with a k off rate calculated to be 15.8 s-1 . SMFS of these biomolecular systems were not only successful and informative, but also provide exciting directions towards future applications.
