Aspect Microbiologique Des Implants A Connectique Cone Morse

Author : Muzzarelli Martina
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (116 download)

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Download or read book In Vitro Evaluation of the Microbial Seal of the Cone-morse Connection written by Muzzarelli Martina and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Background. Tissue inflammation around implants could be due to microbial colonization at the level of implant-abutment interface (IAI). Whereas the complete elimination of the microgap at the IAI is not possible, the design of the implant system could minimize its clinical relevance. Morse-taper implants rely on a frictional connection, which results in a very narrow implant-abutment space, thus increase the sealing capability.Aim. The objective of the present in vitro study was to evaluate bacterial microleakage at the implant-abutment interface in Morse-taper connection implants.Materials and method. Ten implant-abutment samples with a Morse-taper internal connection were used. A bacterial suspension containing three species, involved in the development of peri-implantitis (A. actinomycetemcomitans, F. nucleatum, P. gingivalis), was inoculated into the internal cavity of the samples, except one, which served as negative control. The samples were assembled using a steel support and a calibrated abutment beater. Afterwards, they were totally immersed individually into culture tubes, and incubated at 37u00b0C. One specimen was placed into a growth medium together with the bacterial suspension and served as positive control. Turbidity analysis of nutritional broth, Real-Time PCR, and horizontal electrophoresis on agarose gel at 24 h, 48 h and 72 h were used to determine the microbial outflow through the IAI.Results. One specimen resulted positive to turbidity test, but further test did not confirm bacterial growth of the inoculated bacterial species, suggesting a contamination during the manipulation of the experimental components. One other specimen resulted positive to all performed test, with a bacterial count of 3.61x103 copies/u03bcL at 24 h, due to the outflow of the inoculated bacterial suspension during the assemblation of the implant and the abutment. All tests resulted positive for the positive control, while they were negative for the negative control and all the other specimens.Conclusion and clinical implications. Although in literature no connection type was able to provide a complete bacterial seal at the IAI, Morse-taper connection implants showed less microleakage in comparison with other connection types. The present in vitro evaluation confirmed the sealing capability of Morse-taper connections in static conditions.