Aqueous Two Phase Separation Systems

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Aqueous Two-Phase Systems

Author : Rajni Hatti-Kaul
Publisher : Springer Science & Business Media
ISBN 13 : 1592590284
Total Pages : 442 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Aqueous Two-Phase Systems by : Rajni Hatti-Kaul

Download or read book Aqueous Two-Phase Systems written by Rajni Hatti-Kaul and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.

Partitioning In Aqueous Two – Phase System

Author : Harry Walter
Publisher : Elsevier
ISBN 13 : 0323142915
Total Pages : 731 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Partitioning In Aqueous Two – Phase System by : Harry Walter

Download or read book Partitioning In Aqueous Two – Phase System written by Harry Walter and published by Elsevier. This book was released on 2012-12-02 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

Author : Marco Rito-Palomares
Publisher : Springer
ISBN 13 : 3319593099
Total Pages : 196 pages
Book Rating : 4.3/5 (195 download)

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Book Synopsis Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products by : Marco Rito-Palomares

Download or read book Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products written by Marco Rito-Palomares and published by Springer. This book was released on 2017-07-18 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

Protein Structure Analysis

Author : Roza Maria Kamp
Publisher : Springer Science & Business Media
ISBN 13 : 3642592198
Total Pages : 311 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Protein Structure Analysis by : Roza Maria Kamp

Download or read book Protein Structure Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Ionic-Liquid-Based Aqueous Biphasic Systems

Author : Mara G. Freire
Publisher : Springer
ISBN 13 : 3662528754
Total Pages : 322 pages
Book Rating : 4.6/5 (625 download)

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Book Synopsis Ionic-Liquid-Based Aqueous Biphasic Systems by : Mara G. Freire

Download or read book Ionic-Liquid-Based Aqueous Biphasic Systems written by Mara G. Freire and published by Springer. This book was released on 2016-10-05 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.

Intensification of Biobased Processes

Author : Andrzej Gorak
Publisher : Royal Society of Chemistry
ISBN 13 : 1788010329
Total Pages : 544 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Intensification of Biobased Processes by : Andrzej Gorak

Download or read book Intensification of Biobased Processes written by Andrzej Gorak and published by Royal Society of Chemistry. This book was released on 2018-06-18 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book dedicated entirely to this area, Intensification of Biobased Processes provides a comprehensive overview of modern process intensification technologies used in bioprocessing.

Aqueous Two-Phase Systems

Author : Harry Walter
Publisher : Elsevier
ISBN 13 : 9780121821296
Total Pages : 768 pages
Book Rating : 4.8/5 (212 download)

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Book Synopsis Aqueous Two-Phase Systems by : Harry Walter

Download or read book Aqueous Two-Phase Systems written by Harry Walter and published by Elsevier. This book was released on 1994-04-18 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.

Extractive Bioconversions

Author : B. Mattiasson
Publisher : CRC Press
ISBN 13 : 9780824782726
Total Pages : 348 pages
Book Rating : 4.7/5 (827 download)

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Book Synopsis Extractive Bioconversions by : B. Mattiasson

Download or read book Extractive Bioconversions written by B. Mattiasson and published by CRC Press. This book was released on 1990-11-30 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contributors from European and US universities and corporations review recent developments in the integration of downstream processing and bioconversion, describing their experience with many separation technologies, including some still in the experimental stage. The topics include the construction

Liquid Biphasic System

Author : Pau Loke Show
Publisher : Elsevier
ISBN 13 : 0128223987
Total Pages : 330 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Liquid Biphasic System by : Pau Loke Show

Download or read book Liquid Biphasic System written by Pau Loke Show and published by Elsevier. This book was released on 2020-11-28 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Downstream bioprocesses have a significant role to play in the creation of a sustainable bio-based economy, enabling the creation of new products and systems from the more sustainable bioprocessing of natural products. Liquid Biphasic System: Fundamentals, Methods, and Applications in Bioseparation Technology explores in detail the fundamental processes and applications of this new separation system, aiding understanding of the basic principles of the technique and offering constructive criticisms on the latest findings.Including coverage of the background, principles, mechanisms, and applications, Liquid Biphasic System addresses how to adapt the technology for the purification of useful compounds with greater cost efficiency and greener processing. It is essential reading for bioprocess engineers, biochemical engineers, biosystem engineers, chemists and microbiologists working in the fields of bioprocessing. Researchers, scientists, and engineers concerned with the selection and evaluation of alternative bioseparation processes will find the book particularly useful. - Provides information and examples of advanced separations in a single source - Includes detailed descriptions of novel bioseparation systems - Covers the latest technologies related to advanced liquid–liquid separation and their applications in various industries

The Giant Vesicle Book

Author : Rumiana Dimova
Publisher : CRC Press
ISBN 13 : 1498752187
Total Pages : 652 pages
Book Rating : 4.4/5 (987 download)

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Book Synopsis The Giant Vesicle Book by : Rumiana Dimova

Download or read book The Giant Vesicle Book written by Rumiana Dimova and published by CRC Press. This book was released on 2019-11-19 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt: Giant vesicles are widely used as a model membrane system, both for basic biological systems and for their promising applications in the development of smart materials and cell mimetics, as well as in driving new technologies in synthetic biology and for the cosmetics and pharmaceutical industry. The reader is guided to use giant vesicles, from the formation of simple membrane platforms to advanced membrane and cell system models. It also includes fundamentals for understanding lipid or polymer membrane structure, properties and behavior. Every chapter includes ideas for further applications and discussions on the implications of the observed phenomena towards understanding membrane-related processes. The Giant Vesicle Book is meant to be a road companion, a trusted guide for those making their first steps in this field as well as a source of information required by experts. Key Features • A complete summary of the field, covering fundamental concepts, practical methods, core theory, and the most promising applications • A start-up package of theoretical and experimental information for newcomers in the field • Extensive protocols for establishing the required preparations and assays • Tips and instructions for carefully performing and interpreting measurements with giant vesicles or for observing them, including pitfalls • Approaches developed for investigating giant vesicles as well as brief overviews of previous studies implementing the described techniques • Handy tables with data and structures for ready reference

Aqueous Two-Phase Partitioning

Author : Boris Y. Zaslavsky
Publisher : CRC Press
ISBN 13 : 9780824794613
Total Pages : 720 pages
Book Rating : 4.7/5 (946 download)

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Book Synopsis Aqueous Two-Phase Partitioning by : Boris Y. Zaslavsky

Download or read book Aqueous Two-Phase Partitioning written by Boris Y. Zaslavsky and published by CRC Press. This book was released on 1994-11-15 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.

Separation and Purification Technologies in Biorefineries

Author : Shri Ramaswamy
Publisher : John Wiley & Sons
ISBN 13 : 111849346X
Total Pages : 730 pages
Book Rating : 4.1/5 (184 download)

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Book Synopsis Separation and Purification Technologies in Biorefineries by : Shri Ramaswamy

Download or read book Separation and Purification Technologies in Biorefineries written by Shri Ramaswamy and published by John Wiley & Sons. This book was released on 2013-02-04 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation and purification processes play a critical role in biorefineries and their optimal selection, design and operation to maximise product yields and improve overall process efficiency. Separations and purifications are necessary for upstream processes as well as in maximising and improving product recovery in downstream processes. These processes account for a significant fraction of the total capital and operating costs and also are highly energy intensive. Consequently, a better understanding of separation and purification processes, current and possible alternative and novel advanced methods is essential for achieving the overall techno-economic feasibility and commercial success of sustainable biorefineries. This book presents a comprehensive overview focused specifically on the present state, future challenges and opportunities for separation and purification methods and technologies in biorefineries. Topics covered include: Equilibrium Separations: Distillation, liquid-liquid extraction and supercritical fluid extraction. Affinity-Based Separations: Adsorption, ion exchange, and simulated moving bed technologies. Membrane Based Separations: Microfiltration, ultrafiltration and diafiltration, nanofiltration, membrane pervaporation, and membrane distillation. Solid-liquid Separations: Conventional filtration and solid-liquid extraction. Hybrid/Integrated Reaction-Separation Systems: Membrane bioreactors, extractive fermentation, reactive distillation and reactive absorption. For each of these processes, the fundamental principles and design aspects are presented, followed by a detailed discussion and specific examples of applications in biorefineries. Each chapter also considers the market needs, industrial challenges, future opportunities, and economic importance of the separation and purification methods. The book concludes with a series of detailed case studies including cellulosic bioethanol production, extraction of algae oil from microalgae, and production of biopolymers. Separation and Purification Technologies in Biorefineries is an essential resource for scientists and engineers, as well as researchers and academics working in the broader conventional and emerging bio-based products industry, including biomaterials, biochemicals, biofuels and bioenergy.

Open-Space Microfluidics

Author : Emmanuel Delamarche
Publisher : John Wiley & Sons
ISBN 13 : 3527696792
Total Pages : 450 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Open-Space Microfluidics by : Emmanuel Delamarche

Download or read book Open-Space Microfluidics written by Emmanuel Delamarche and published by John Wiley & Sons. This book was released on 2018-01-18 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.

Separation Technologies for the Industries of the Future

Author : Panel on Separation Technology for Industrial Reuse and Recycling
Publisher : National Academies Press
ISBN 13 : 0309592828
Total Pages : 128 pages
Book Rating : 4.3/5 (95 download)

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Book Synopsis Separation Technologies for the Industries of the Future by : Panel on Separation Technology for Industrial Reuse and Recycling

Download or read book Separation Technologies for the Industries of the Future written by Panel on Separation Technology for Industrial Reuse and Recycling and published by National Academies Press. This book was released on 1999-01-22 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation processesor processes that use physical, chemical, or electrical forces to isolate or concentrate selected constituents of a mixtureare essential to the chemical, petroleum refining, and materials processing industries. In this volume, an expert panel reviews the separation process needs of seven industries and identifies technologies that hold promise for meeting these needs, as well as key technologies that could enable separations. In addition, the book recommends criteria for the selection of separations research projects for the Department of Energy's Office of Industrial Technology.

Bioseparation

Author : G.T. Tsao
Publisher : Springer
ISBN 13 : 9783662149881
Total Pages : 200 pages
Book Rating : 4.1/5 (498 download)

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Book Synopsis Bioseparation by : G.T. Tsao

Download or read book Bioseparation written by G.T. Tsao and published by Springer. This book was released on 2014-03-12 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Microorganisms In Industry And Environment: From Scientific And Industrial Research To Consumer Products - Proceedings Of The Iii International Conference On Environmental, Industrial And Applied Microbiology (Biomicroworld2009)

Download Microorganisms In Industry And Environment: From Scientific And Industrial Research To Consumer Products - Proceedings Of The Iii International Conference On Environmental, Industrial And Applied Microbiology (Biomicroworld2009) PDF Online Free

Author : Antonio Mendez-vilas
Publisher : World Scientific
ISBN 13 : 9814464198
Total Pages : 736 pages
Book Rating : 4.8/5 (144 download)

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Book Synopsis Microorganisms In Industry And Environment: From Scientific And Industrial Research To Consumer Products - Proceedings Of The Iii International Conference On Environmental, Industrial And Applied Microbiology (Biomicroworld2009) by : Antonio Mendez-vilas

Download or read book Microorganisms In Industry And Environment: From Scientific And Industrial Research To Consumer Products - Proceedings Of The Iii International Conference On Environmental, Industrial And Applied Microbiology (Biomicroworld2009) written by Antonio Mendez-vilas and published by World Scientific. This book was released on 2010-12-09 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to disseminate the most current research in applied microbiology presented at the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009) held in Lisbon, Portugal, in December 2009.This volume offers an inviting exploration of microbiology from scientific and industrial research to consumer products in a compilation of more than 150 papers written by leading experts in the field, who afford critical insights into several topics, review current research and discuss future directions to stimulate further discussions. This book also serves as an update on the most important current microbial research, by providing a comprehensive overview of cutting-edge topics in a single volume, where readers can also gain insights into how microbiology can solve problems in everyday settings.Although largely intended for microbiologists interested in knowing the latest developments in agriculture, environmental, food, industrial, medical and pharmaceutical microbiology and microbial biotechnology, this book is also a great source of reference for scientists and researchers involved in advancements in applied microbiology.

Separation Processes in Biotechnology

Author : Juan A. Asenjo
Publisher : CRC Press
ISBN 13 : 100010432X
Total Pages : 824 pages
Book Rating : 4.0/5 (1 download)

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Book Synopsis Separation Processes in Biotechnology by : Juan A. Asenjo

Download or read book Separation Processes in Biotechnology written by Juan A. Asenjo and published by CRC Press. This book was released on 2020-08-26 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited to avoid duplication and favor comprehensiveness, 20 contributors detail the recovery, separation, and purification operations of bioprocess technology. Individual chapters in this classic yet still highly relevant work emphasize concepts that are becoming more and more important when applied to the large scale versions of techniques that are considered well established. Aside from fully discussing processes, Separation Processes in Biotechnology includes sections on concentration separation and operation, purification operations, and product release and recovery. It also discusses plant operation and equipment and delves into economic considerations