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Application Of Non Targeted Volatile Metabolomics In Plant Pathology
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Book Synopsis Application of Non-targeted Volatile Metabolomics in Plant Pathology by : Jinyan She
Download or read book Application of Non-targeted Volatile Metabolomics in Plant Pathology written by Jinyan She and published by . This book was released on 2017 with total page 203 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our study focuses on the application of volatile metabolomics and chemometrics in plant pathology. Chapter one provides an overview of mass spectrometry based volatile metabolomics. The study of volatile metabolites profiles from virulent and hypovirulent strains of C. parasitica are presented in chapter two. The microbial volatile organic compounds (MVOCs) profiles were analyzed via headspace solid phase microextraction (HS-SPME) and gas chromatography (GC)-mass spectrometry (MS). The results indicate that the MVOCs from these two strains are significantly different. Furthermore, the study explored MVOCs differences associated with hypovirulence processes. The study found that both hypovirulence and aging can alter the virulent strains' MVOCs, and the process can be observed via their volatile metabolites. Chapter three describes the effects of aging, cultivation medium, and pH on fungal volatile metabolite profiles, all of which can change the strength of MVOCs emission and their composition. An acidic environment favors fungal bioactivity and therefore enhanced MVOCs emission. However, due to the inherently low MVOCs production from hypovirulent strains, the pH effect was less apparent in the hypovirulent isolates. The strength of MVOCs emission was highly correlated to the fungal expansion in virulent strains for the first 14 days. Finally, the cultivation media are critical to the fungal MVOCs production. Among the tested media, cornmeal was least favorable for MVOCs production for both strains. Chapter Four presents a study of the total constitutive phenolic content estimation and volatile organic compounds identification from four species of chestnut tree leaf tissues. Folin Ciocalteu reagent assay with UV/Vis spectrophotometry was applied to estimate the total phenolic content in leaf tissues of American chestnut (Castanea dentata), Chinese chestnut (Castanea mollissima), and their backcross breeding generations (B3F2 and B3F3). The results show that the variations among these tree species are significant (ANOVA, p
Book Synopsis Plant Metabolomics by : Kazuki Saito
Download or read book Plant Metabolomics written by Kazuki Saito and published by Springer Science & Business Media. This book was released on 2006-06-29 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics – which deals with all metabolites of an organism – is a rapidly-emerging sector of post-genome research fields. It plays significant roles in a variety of fields from medicine to agriculture and holds a fundamental position in functional genomics studies and their application in plant biotechnology. This volume comprehensively covers plant metabolomics for the first time. The chapters offer cutting-edge information on analytical technology, bioinformatics and applications. They were all written by leading researchers who have been directly involved in plant metabolomics research throughout the world. Up-to-date information and future developments are described, thereby producing a volume which is a landmark of plant metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all plant science fields.
Book Synopsis Food Authentication by : Contantinos A. Georgiou
Download or read book Food Authentication written by Contantinos A. Georgiou and published by John Wiley & Sons. This book was released on 2017-05-08 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: The determination of food authenticity is a vital component of quality control. Its importance has been highlighted in recent years by high-profile cases in the global supply chain such as the European horsemeat scandal and the Chinese melamine scandal which led to six fatalities and the hospitalisation of thousands of infants. As well as being a safety concern, authenticity is also a quality criterion for food and food ingredients. Consumers and retailers demand that the products they purchase and sell are what they purport to be. This book covers the most advanced techniques used for the authentication of a vast number of products around the world. The reader will be informed about the latest pertinent analytical techniques. Chapters focus on the novel techniques & markers that have emerged in recent years. An introductory section presents the concepts of food authentication while the second section examines in detail the analytical techniques for the detection of fraud relating to geographical, botanical, species and processing origin and production methods of food materials and ingredients. Finally, the third section looks at consumer attitudes towards food authenticity, the application of bioinformatics to this field, and the Editor’s conclusions and future outlook. Beyond being a reference to researchers working in food authentication it will serve as an essential source to analytical scientists interested in the field and food scientists to appreciate analytical approaches. This book will be a companion to under- and postgraduate students in their wander in food authentication and aims to be useful to researchers in universities and research institutions.
Book Synopsis Methodologies for Metabolomics by : Norbert W. Lutz
Download or read book Methodologies for Metabolomics written by Norbert W. Lutz and published by Cambridge University Press. This book was released on 2013-01-21 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.
Book Synopsis Volatiles and Metabolites of Microbes by : Joginder Singh Panwar
Download or read book Volatiles and Metabolites of Microbes written by Joginder Singh Panwar and published by Academic Press. This book was released on 2021-06-22 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volatiles and Metabolites of Microbes compiles the latest research and advancement in the field of volatiles, metabolites synthesized from the microbial strains such as actinomycetes, bacteria, cyanobacteria, and fungal species and their potential applications in the field of healthcare issue and sustainable agriculture. There is an urgent need to explore new and advanced biological methods for health industries and sustainable agriculture and to protect the environment from environmental pollution or contaminates, global warming, and also control the health of human beings from the side effects of various pharmaceuticals products. Focusing all these factors, Volatiles and Metabolites of Microbes explores new aspects of microorganism in terms of volatiles, enzymes, bioactive compounds synthesized from the microbes and their potential applications in the field of sustainable agriculture and health-related issues - Provides a broad aspect about volatiles, bioactive compounds, and secondary metabolites of microbes compiled in one cover - Gives the latest research and advancement in the field of volatiles, secondary metabolites, and bioactive compounds synthesized from the different microbial strains - Responds to new developments in the detection of the complex compound structures of volatiles - Offers insight to a very broad audience in Biotechnology, Applied Microbiology, Agronomy, and Pathology
Book Synopsis High-Throughput Metabolomics by : Angelo D'Alessandro
Download or read book High-Throughput Metabolomics written by Angelo D'Alessandro and published by Humana. This book was released on 2020-06-06 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.
Book Synopsis New Frontiers in Stress Management for Durable Agriculture by : Amitava Rakshit
Download or read book New Frontiers in Stress Management for Durable Agriculture written by Amitava Rakshit and published by Springer Nature. This book was released on 2020-03-23 with total page 663 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using accessible farming practices to meet the growing demands on agriculture is likely to result in more intense competition for natural resources, increased greenhouse gas emissions, and further deforestation and land degradation, which will in turn produce additional stress in the soil-water-plant-animal continuum. Stress refers to any unfavorable force or condition that inhibits customary functioning in plants. Concurrent manifestations of different stresses (biotic and abiotic) are very frequent in the environment of plants, which consequently reduces yield. Better understanding stress not only changes our perspective on the current environment, but can also bring a wealth of benefits, like improving sustainable agriculture and human beings’ living standards. Innovative systems are called for that protect and enhance the natural resource base, while increasing productivity via ‘holistic’ approaches, such as agroecology, agro-forestry, climate-smart agriculture and conservation agriculture, which also incorporate indigenous and traditional knowledge. The book ‘New Frontiers in Stress Management for Durable Agriculture’ details the current state of knowledge and highlights scientific advances concerning novel aspects of plant biology research on stress, biotic and abiotic stress responses, as well as emergent amelioration and reclamation technologies to restore normal functioning in agroecology.
Book Synopsis Computational Methods and Data Analysis for Metabolomics by :
Download or read book Computational Methods and Data Analysis for Metabolomics written by and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lignins written by Kyosti V. Sarkanen and published by John Wiley & Sons. This book was released on 1971 with total page 952 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis High-Throughput Phenotyping in Plants by : Jennifer Normanly
Download or read book High-Throughput Phenotyping in Plants written by Jennifer Normanly and published by Humana Press. This book was released on 2017-04-30 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, High Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.
Book Synopsis Principles of Plant-Microbe Interactions by : Ben Lugtenberg
Download or read book Principles of Plant-Microbe Interactions written by Ben Lugtenberg and published by Springer. This book was released on 2014-12-04 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of microbial plant protection products is growing and their importance will strongly increase due to political and public pressure. World population is growing and the amount of food needed by 2050 will be double of what is produced now whereas the area of agricultural land is decreasing. We must increase crop yield in a sustainable way. Chemical plant growth promoters must be replaced by microbiological products. Also here, the use of microbial products is growing and their importance will strongly increase. A growing area of agricultural land is salinated. Global warming will increase this process. Plants growth is inhibited by salt or even made impossible and farmers tend to disuse the most salinated lands. Microbes have been very successfully used to alleviate salt stress of plants. Chemical pollution of land can make plant growth difficult and crops grown are often polluted and not suitable for consumption. Microbes have been used to degrade these chemical pollutants.
Book Synopsis Metabolomics in Practice by : Michael Lämmerhofer
Download or read book Metabolomics in Practice written by Michael Lämmerhofer and published by John Wiley & Sons. This book was released on 2013-02-14 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment. Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technology in the academic and industrial research laboratory, the lessons taught here are highly relevant for all systems-level approaches, whether in systems biology, biotechnology, toxicology or pharmaceutical sciences. From the Contents: * Sampling and Sample Preparation in Microbial Metabolomics * Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics * GC-MS, LC-MS, CE-MS and Ultrahigh Resolution MS (FTICR-MS) in Metabolomics * NMR-based metabolomics analysis * Potential of Microfluidics and Single Cell Analysis in Metabolomics * Data Processing in Metabolomics * Validation and Measurement Uncertainty in Metabolomic Studies * Metabolomics and its Role in the Study of Mammalian Systems and in Plant Sciences * Metabolomics in Biotechnology and Nutritional Metabolomics and more.
Book Synopsis Plant Metabolites and Regulation under Environmental Stress by : Parvaiz Ahmad
Download or read book Plant Metabolites and Regulation under Environmental Stress written by Parvaiz Ahmad and published by Academic Press. This book was released on 2018-03-19 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Metabolites and Regulation Under Environmental Stress presents the latest research on both primary and secondary metabolites. The book sheds light on the metabolic pathways of primary and secondary metabolites, the role of these metabolites in plants, and the environmental impact on the regulation of these metabolites. Users will find a comprehensive, practical reference that aids researchers in their understanding of the role of plant metabolites in stress tolerance. Highlights new advances in the understanding of plant metabolism Features 17 protocols and methods for analysis of important plant secondary metabolites Includes sections on environmental adaptations and plant metabolites, plant metabolites and breeding, plant microbiome and metabolites, and plant metabolism under non-stress conditions
Book Synopsis Current Protocols in Molecular Biology by :
Download or read book Current Protocols in Molecular Biology written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in Gas Chromatography by : Xinghua Guo
Download or read book Advances in Gas Chromatography written by Xinghua Guo and published by BoD – Books on Demand. This book was released on 2014-02-26 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: For decades gas chromatography has been and will remain an irreplaceable analytical technique in many research areas for both quantitative analysis and qualitative characterization/identification, which is still supplementary with HPLC. This book highlights a few areas where significant advances have been reported recently and/or a revisit of basic concepts is deserved. It provides an overview of instrumental developments, frontline and modern research as well as practical industrial applications. The topics include GC-based metabolomics in biomedical, plant and microbial research, natural products as well as characterization of aging of synthetic materials and industrial monitoring, which are contributions of several experts from different disciplines. It also contains best hand-on practices of sample preparation (derivatization) and data processing in daily research. This book is recommended to both basic and experienced researchers in gas chromatography.
Book Synopsis Plant-derived Natural Products by : Anne E. Osbourn
Download or read book Plant-derived Natural Products written by Anne E. Osbourn and published by Springer Science & Business Media. This book was released on 2009-07-07 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants produce a huge array of natural products (secondary metabolites). These compounds have important ecological functions, providing protection against attack by herbivores and microbes and serving as attractants for pollinators and seed-dispersing agents. They may also contribute to competition and invasiveness by suppressing the growth of neighboring plant species (a phenomenon known as allelopathy). Humans exploit natural products as sources of drugs, flavoring agents, fragrances and for a wide range of other applications. Rapid progress has been made in recent years in understanding natural product synthesis, regulation and function and the evolution of metabolic diversity. It is timely to bring this information together with contemporary advances in chemistry, plant biology, ecology, agronomy and human health to provide a comprehensive guide to plant-derived natural products. Plant-derived natural products: synthesis, function and application provides an informative and accessible overview of the different facets of the field, ranging from an introduction to the different classes of natural products through developments in natural product chemistry and biology to ecological interactions and the significance of plant-derived natural products for humans. In the final section of the book a series of chapters on new trends covers metabolic engineering, genome-wide approaches, the metabolic consequences of genetic modification, developments in traditional medicines and nutraceuticals, natural products as leads for drug discovery and novel non-food crops.
Book Synopsis Secondary Metabolites of Plant Growth Promoting Rhizomicroorganisms by : Harikesh Bahadur Singh
Download or read book Secondary Metabolites of Plant Growth Promoting Rhizomicroorganisms written by Harikesh Bahadur Singh and published by Springer. This book was released on 2019-04-15 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent changes in the pattern of agricultural practices from use of hazardous pesticides to natural (organic) cultivation has brought into focus the use of agriculturally important microorganisms for carrying out analogous functions. The reputation of plant growth promoting rhizomicroorganisms (PGPRs) is due to their antagonistic mechanisms against most of the fungal and bacterial phytopathogens. The biocontrol potential of agriculturally important microorganisms is mostly attributed to their bioactive secondary metabolites. However, low shelf life of many potential agriculturally important microorganisms impairs their use in agriculture and adoption by farmers. The focal theme of this book is to highlight the potential of employing biosynthesized secondary metabolites (SMs) from agriculturally important microorganisms for management of notorious phytopathogens, as a substitute of the currently available whole organism formulations and also as alternatives to hazardous synthetic pesticides. Accordingly, we have incorporated a comprehensive rundown of sections which particularly examine the SMs synthesized, secreted and induced by various agriculturally important microorganisms and their applications in agriculture. Section 1 includes discussion on biosynthesized antimicrobial secondary metabolites from fungal biocontrol agents. This section will cover the various issues such as development of formulation of secondary metabolites, genomic basis of metabolic diversity, metabolomic profiling of fungal biocontrol agents, novel classes of antimicrobial peptides. The section 1 will also cover the role of these secondary metabolites in antagonist-host interaction and application of biosynthesized antimicrobial secondary metabolites for management of plant diseases. Section 2 will discuss the biosynthesized secondary metabolites from bacterial PGPRs, strain dependent effects on plant metabolome profile, bio-prospecting various isolates of bacterial PGPRs for potential secondary metabolites and non-target effects of PGPR on microbial community structure and functions. Section 3 encompasses synthesis of antimicrobial secondary metabolites from beneficial endophytes, bio-prospecting medicinal and aromatic hosts and effect of endophytic SMs on plants under biotic and biotic stress conditions.
