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Analyses Structurales De Proteines Et De Complexes De Coordination Par Spectrometrie De Masse
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Book Synopsis ANALYSES STRUCTURALES DE PROTEINES ET DE COMPLEXES DE COORDINATION PAR SPECTROMETRIE DE MASSE by : FRANCIS.. BITSCH
Download or read book ANALYSES STRUCTURALES DE PROTEINES ET DE COMPLEXES DE COORDINATION PAR SPECTROMETRIE DE MASSE written by FRANCIS.. BITSCH and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: LE BUT DE NOTRE THESE A ETE DE DEVELOPPER DES METHODES ET DE STRATEGIES D'ANALYSES STRUCTURALES DE PEPTIDES, DE PROTEINES ET DE COMPLEXES DE COORDINATION PAR SPECTROMETRIE DE MASSE. L'ETUDE DES MASSIFS ISOTOPIQUES NOUS A PERMIS DE DETERMINER LES CONDITIONS D'UTILISATION DES MESURES DE MASSE MONOISOTOPIQUES ET MOYENNES. NOUS AVONS ETUDIE DANS QUELLES CONDITIONS CES MESURES PERMETTAIENT DE METTRE EN EVIDENCE DES MODIFICATIONS COURAMMENT RENCONTREES SUR DES PROTEINES. L'INTERPRETATION DES FRAGMENTATIONS DES IONS PEPTIDIQUES EN LSIMS NOUS A PERMIS D'OBTENIR DES INFORMATIONS DE SEQUENCE PEPTIDIQUE SUR DES VARIANTS DE L'HIRUDINE RECOMBINANTE AINSI QUE SUR DES PEPTIDES DE COUPURES DE LA PROTAMINE SP DE SEICHE. L'ETUDE DE LA FRAGMENTATIONS DES LIAISONS DISULFURES EN LSIMS NOUS ONT PERMIS DE PROPOSER UNE NOUVELLE STRATEGIE DANS L'ATTRIBUTION DES PONTS DISULFURES; CELLE-CI A ETE APPLIQUEE A LA DETERMINATION DES PONTS DISULFURES DE LA DEFENSINE A NATURELLE ET RECOMBINANTE. NOUS AVONS APPLIQUE L'IONISATION PAR ELECTROSPRAY (ES-MS) A LA CARACTERISATION DE PEPTIDES ET DE PROTEINES INTACTES, NATURELLES OU PRODUITES PAR LA SYNTHESE CHIMIQUE OU LE GENIE GENETIQUE. L'UTILISATION DE LA FAB-MS ET DE L'ES-MS NOUS A PERMIS D'ELUCIDER LES STRUCTURES DE COMPLEXES DE COORDINATION MACROMOLECULAIRES (CARTENATES) POUR LESQUELS LA CARACTERISATION EST DIFFICILE PAR LES METHODES ACTUELLES D'ANALYSE (R.M.N.)
Book Synopsis Nouvelles perspectives pour l'analyse et la caractérisation structurale de complexes biologiques par spectrométrie de masse supramoléculaire by : Cédric Atmanene
Download or read book Nouvelles perspectives pour l'analyse et la caractérisation structurale de complexes biologiques par spectrométrie de masse supramoléculaire written by Cédric Atmanene and published by . This book was released on 2009 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: La spectrométrie de masse supramoléculaire (ou MS des complexes non-covalents) est une technique biophysique de plus en plus sollicitée pour la caractérisation des interactions entre biomolécules. Afin d’étendre les potentialités de cette approche pour l’étude d’édifices biologiques non-covalents plus complexes, le travail réalisé au cours de cette thèse s’articule autour de trois axes :• L’automatisation des analyses nanoelectrospray sur puce microfluidique : en augmentant le débit d’analyses tout en réduisant la consommation d’échantillon, nos développements ont renforcé l’intérêt de la MS supramoléculaire pour la caractérisation détaillée de complexes biologiques ainsi que pour le criblage des ligands de protéines cibles.• La caractérisation de complexes protéine/ARN : les stratégies analytiques développées ont permis d’étendre le champ d’applications de la MS supramoléculaire à cette catégorie de complexes, démontrant ainsi la possibilité d’accéder à des informations concernant leur stœchiométrie ou encore leurs réseaux d’interactions.• Le couplage entre spectrométrie de mobilité ionique et MS : les développements méthodologiques réalisés ont permis d’atteindre un niveau supplémentaire dans la caractérisation de complexes biologiques avec la possibilité de différencier des conformères et d’estimer leur section efficace.Ce travail de thèse démontre la versatilité de l’approche de MS supramoléculaire qui se traduit par la diversité des complexes étudiés (protéine/ligand, protéine/oligonucléotide, etc.), ainsi que par la diversité des informations accessibles (stœchiométries, spécificités d’interaction, affinités, dynamiques d’assemblage et conformationnelles, etc.).
Book Synopsis Développement de nouvelles méthodologies par spectrométrie de masse à très haute résolution pour l'analyse structurale de complexes protéiques by : Ophélie Robine
Download or read book Développement de nouvelles méthodologies par spectrométrie de masse à très haute résolution pour l'analyse structurale de complexes protéiques written by Ophélie Robine and published by . This book was released on 2012 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: L'association échanges hydrogène/deutérium et spectrométrie de masse (HDX/MS) est devenue un outil analytique de choix pour l'étude de la structure et dynamique des protéines. L'approche classique "bottom-up" repose sur la digestion à la pepsine de la protéine deutérée et analyse des peptides protéolytiques par MS. Malheureusement, cette approche souffre de deux limitations majeures : (1) la résolution limitée par la taille des peptides après digestion et (2) l'échange inverse en solution avant l'analyse par MS. La MS en tandem classique ne peut pas être utilisée pour améliorer la résolution depuis que des expériences CID (Collision Induced Dissociation) sur des peptides deutérés ont montré une migration intramoléculaire des deutériums avant dissociation ("scrambling"). Par conséquent, il y a un grand intérêt à développer des approches HDX/MS alternatives pour améliorer les problèmes d'échange inverse, scrambling et résolution. Ce travail a donc consisté à mettre au point une méthode top-down HDX/MS robuste. Un nouveau système d'introduction des protéines deutérées dans le spectromètre de masse, appelé "cryosource", a été développé et les techniques d'activation basées sur la capture/transfert d'électron ont été utilisées. Il a récemment été montré que les méthodes ECD/ETD réduisaient le scrambling dans des conditions contrôlées avec une résolution à l'acide aminé près. L'utilisation de la cryosource a permis de diminuer l'échange inverse pendant des temps longs et avec des débits faibles. L'optimisation de tous les paramètres a été réalisée sur des peptides/protéine modèles. Notre objectif final était que notre approche soit applicable à l'analyse structurale du complexe aIF2
Book Synopsis Novel Mass Spectrometry-based Strategies for Biophysical Analysis of Proteins and Protein-ligand Complexes by : Liangjie Tang
Download or read book Novel Mass Spectrometry-based Strategies for Biophysical Analysis of Proteins and Protein-ligand Complexes written by Liangjie Tang and published by ProQuest. This book was released on 2007 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third protocol developed here is a SUPREX-like protocol that employs a chemical modification reaction (i.e., oxidation reaction) in place of the amide H/D exchange reaction in SUPREX. This SUPREX-like protocol, termed SPROX (Stability of Proteins from Rates of Oxidation) was developed and tested to detect and quantify protein-ligand binding using cyclophilin A and BCAII as model systems.
Download or read book Biochemistry and Cell Biology written by and published by . This book was released on 2008 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Elemental Speciation in Human Health Risk Assessment by : P. Apostoli
Download or read book Elemental Speciation in Human Health Risk Assessment written by P. Apostoli and published by World Health Organization. This book was released on 2006 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: Definitions of species and speciation - - Strructural aspects of speciation - - Analytical techniques and methodology - - Bioaccessibility and bioavailability - - Toxicokinetics and biological monitoring - - Molecular and cellular mechanisms of metal toxicity - - Health effects - - Conclusions and recommendations.
Book Synopsis Circular Dichroism and Linear Dichroism by : Alison Rodger
Download or read book Circular Dichroism and Linear Dichroism written by Alison Rodger and published by Oxford University Press, USA. This book was released on 1997 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to all those who wish to use the complementary spectroscopic techniques of optical activity (circular dichroism, CD) and optical anisotropy (linear dichroism, LD) for the study of the structure of molecules and interactions between molecules in solution. It emphasizes these techniques and how to use them for both low and high molecular weight molecules. The book begins by describing the principles behind CD and LD and how these techniques can be used in the laboratory without using advanced maths or quantum mechanics. The next chapters describe how both techniques may be applied to the study of biological macromolecules and give a detailed description of how they may be used on small molecules to investigate molecular and electronic structure. The final part contains theoretical derivations of all the equations required for the applications described previously. Specific molecular examples are used to illustrate concepts and to show the reader how to use the techniques in chemical and biological systems. Circular Dichroism and Linear Dichroism is an easy guide to what a prospective user of CD needs to know and explains how LD is not merely an exotic technique only to be practiced by experienced spectroscopists, but may be routinely and usefully employed as an aid to molecular structure determination.
Book Synopsis Plant-microbe Interactions 2 by : Gary Stacey
Download or read book Plant-microbe Interactions 2 written by Gary Stacey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant-Microbe Interactions, Volume 2 Volume 1 of this series has made its appearance and dealt forcefully with impor tant current topics in the field of plant-microbe interactions. We believe that the quality of those chapters was high and should serve as a focal point for the state of the art as well as an enduring reference. Volume 2 builds upon these accom plishments. Chapter 1 discusses the fascinating lipo-chitin signal molecules from Rhizo bium, aspects regarding their biosynthesis, and the basis for host specificity. These molecules are a cardinal example of how microorganisms influence plant development and stimulate speculation that they have identified a previously un known aspect of plant hormone activity. Chapter 2 continues the discussion of Rhizobium by considering the trafficking of carbon and nitrogen in nodules. Al though the ostensible advantage of nodules to plants is the fixation of atmos pheric nitrogen, the actual process involved in supplying reduced nitrogen to the plant host is complex.
Book Synopsis Bioaffinity Chromatography by : J. Turková
Download or read book Bioaffinity Chromatography written by J. Turková and published by Elsevier. This book was released on 1993-09-14 with total page 819 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography.Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.
Book Synopsis Plant Based "Green Chemistry 2.0" by : Ying Li
Download or read book Plant Based "Green Chemistry 2.0" written by Ying Li and published by . This book was released on 2019 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides practical information on obtaining and using a wide variety of plant based reagents for different sectors, addressing the needs and challenges in a single resource. The chapters complement each other seamlessly and present contributions from reputed international researchers and renowned professionals from industry, covering the latest efforts in the field. The book serves as the starting point for future collaborations in the new area "Plant Based Green Chemistry" between research, industry, and education, covering large ecologic and economic applications: perfume, cosmetic, pharmaceutical, food ingredients, nutraceuticals, biofuels, or fine chemicals industries. This book is aimed at professionals from industries, academicians engaged in plant based green chemistry, researchers and graduate level students, but will also be useful to food technologists and students and researchers involved in natural products chemistry.
Download or read book Lasso Peptides written by Yanyan Li and published by Springer. This book was released on 2014-10-21 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lasso peptides form a growing family of fascinating ribosomally-synthesized and post-translationally modified peptides produced by bacteria. They contain 15 to 24 residues and share a unique interlocked topology that involves an N-terminal 7 to 9-residue macrolactam ring where the C-terminal tail is threaded and irreversibly trapped. The ring results from the condensation of the N-terminal amino group with a side-chain carboxylate of a glutamate at position 8 or 9, or an aspartate at position 7, 8 or 9. The trapping of the tail involves bulky amino acids located in the tail below and above the ring and/or disulfide bridges connecting the ring and the tail. Lasso peptides are subdivided into three subtypes depending on the absence (class II) or presence of one (class III) or two (class I) disulfide bridges. The lasso topology results in highly compact structures that give to lasso peptides an extraordinary stability towards both protease degradation and denaturing conditions. Lasso peptides are generally receptor antagonists, enzyme inhibitors and/or antibacterial or antiviral (anti-HIV) agents. The lasso scaffold and the associated biological activities shown by lasso peptides on different key targets make them promising molecules with high therapeutic potential. Their application in drug design has been exemplified by the development of an integrin antagonist based on a lasso peptide scaffold. The biosynthesis machinery of lasso peptides is therefore of high biotechnological interest, especially since such highly compact and stable structures have to date revealed inaccessible by peptide synthesis. Lasso peptides are produced from a linear precursor LasA, which undergoes a maturation process involving several steps, in particular cleavage of the leader peptide and cyclization. The post-translational modifications are ensured by a dedicated enzymatic machinery, which is composed of an ATP-dependent cysteine protease (LasB) and a lactam synthetase (LasC) that form an enzymatic complex called lasso synthetase. Microcin J25, produced by Escherichia coli AY25, is the archetype of lasso peptides and the most extensively studied. To date only around forty lasso peptides have been isolated, but genome mining approaches have revealed that they are widely distributed among Proteobacteria and Actinobacteria, particularly in Streptomyces, making available a rich resource of novel lasso peptides and enzyme machineries towards lasso topologies.
Book Synopsis Detection Methods in Precision Medicine by : Mengsu Yang
Download or read book Detection Methods in Precision Medicine written by Mengsu Yang and published by Royal Society of Chemistry. This book was released on 2020-12-14 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will be among the first to cover the detection methods for precision medicine that are set to transform health care in the future.
Book Synopsis Dromadaires et chameaux, animaux laitiers by : Pascal Bonnet
Download or read book Dromadaires et chameaux, animaux laitiers written by Pascal Bonnet and published by Editions Quae. This book was released on 1998 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Linear Dichroism and Circular Dichroism by : Bengt Nordén
Download or read book Linear Dichroism and Circular Dichroism written by Bengt Nordén and published by Royal Society of Chemistry. This book was released on 2019-05-02 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to optical anisotropy (linear dichroism, LD) and optical activity (circular dichroism, CD) as techniques for the study of structures and interactions of molecules in solution. The book covers the use of these techniques for both small and large molecular systems with particular emphasis being placed on proteins and nucleic acids. CD is a well-established technique and this book aims to explain how it can be used simply and effectively for new entrants to the field as well as covering more advanced techniques for experts. LD is often seen as a rather exotic method intended only for experienced spectroscopists. This book demonstrates that it is an approach with real utility that may be used by both students and scientists from graduate level onwards to give simple answers, which are not available from any other technique, to structural and kinetic questions. Much of the emphasis is on flow orientation of samples in solution phase. The book first describes the techniques and the information they can provide; it then goes on to give specific details on how to actually implement them, including a wide range of examples showing how LD and CD can help with * protein and nucleic acid secondary structure elucidation; * analysis of the formation and rearrangements of fibrous proteins and membrane proteins; * identification of the absolute configuration of small molecules; * determination of the orientation of small molecules in anisotropic media; * assignment of transition moment polarizations; * investigation of binding strengths and geometries of ligand-macromolecule complexes; * 3-D structure determination from LD, molecular replacement and MD modeling. The advantages of combined LD/CD studies are also outlined with examples of DNA/drug complexes and protein insertion into membranes. Taken together the book represents a comprehensive text on the theory and application of LD and CD in the chemical and biological sciences.
Book Synopsis Modern Magnetic Resonance by : Graham A. Webb
Download or read book Modern Magnetic Resonance written by Graham A. Webb and published by Springer Science & Business Media. This book was released on 2007-05-26 with total page 1889 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive collection of the applications of Nuclear Magnetic Resonance (NMR), Magnetic Resonance Imaging (MRI) and Electron-Spin Resonance (ESR). Covers the wide ranging disciplines in which these techniques are used: * Chemistry; * Biological Sciences; * Pharmaceutical Sciences; * Medical uses; * Marine Science; * Materials Science; * Food Science. Illustrates many techniques through the applications described, e.g.: * High resolution solid and liquid state NMR; * Low resolution NMR, especially important in food science; * Solution State NMR, especially important in pharmaceutical sciences; * Magnetic Resonance Imaging, especially important for medical uses; * Electron Spin Resonance, especially important for spin-labelling in food, marine and medical studies.
Book Synopsis Chiral Separations by : Bezhan Chankvetadze
Download or read book Chiral Separations written by Bezhan Chankvetadze and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Scientific Basis for Nuclear Waste Management XXII: Volume 556 by : David J. Wronkiewicz
Download or read book Scientific Basis for Nuclear Waste Management XXII: Volume 556 written by David J. Wronkiewicz and published by . This book was released on 1999-11-24 with total page 1370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Safe and effective management of nuclear waste provides a broad range of challenges for materials science. Waste processing, waste form and engineered barrier properties, interactions between engineered and geological systems, radiation effects, chemistry and transport of waste species, and long-term predictions of repository performance are just some of the scientific problems facing modern society. This book, the 22nd in a very successful series from MRS, offers an international and inter-disciplinary perspective on the issues, and features developments in both fundamental and applied areas. Topics include: development and characterization of ceramic waste forms; ceramic waste form corrosion; glass waste form processing; glass formulation, properties and structure; glass waste form corrosion; spent nuclear fuel; performance assessment; repository backfill; flow and transport; natural analogues; container corrosion; metal waste form corrosion; radionuclide speciation and solubility; radionuclide sorption; microbial effects; radiation effects; cement waste forms and waste treatment.
