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Author : Yen-Ho Chu
Publisher :
ISBN 13 :
Total Pages : 394 pages
Book Rating : 4.:/5 (296 download)
Download or read book Affinity Electrophoresis in Capillaries and Gels written by Yen-Ho Chu and published by . This book was released on 1993 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Andras Guttman
Publisher : Newnes
ISBN 13 : 0080931359
Total Pages : 391 pages
Book Rating : 4.0/5 (89 download)
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Author : Reinhard H. H. Neubert
Publisher : CRC Press
ISBN 13 : 0203912136
Total Pages : 392 pages
Book Rating : 4.2/5 (39 download)
Download or read book Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics written by Reinhard H. H. Neubert and published by CRC Press. This book was released on 2003-01-14 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference presents the most recent breakthroughs and techniques in affinity capillary electrophoresis (ACE) to measure and determine the physicochemical and thermodynamic parameters of drug compounds. The authors offer strategies to explore and characterize interactions between drugs, drug vehicles, and biological membranes to facilitate devel
Author : Source Wikipedia
Publisher : University-Press.org
ISBN 13 : 9781230478975
Total Pages : 100 pages
Book Rating : 4.4/5 (789 download)
Download or read book Electrophoresis written by Source Wikipedia and published by University-Press.org. This book was released on 2013-09 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 42. Chapters: Affinity electrophoresis, Capillary electrochromatography, Capillary electrophoresis, Color marker, Comet assay, Denaturing gel electrophoresis, Dielectrophoresis, Difference gel electrophoresis, DNA laddering, DNA separation by silica adsorption, Electrical mobility, Electrophoresis (journal), Electrophoretic mobility shift assay, Electrophoretogram, Gel electrophoresis of nucleic acids, Gel electrophoresis of proteins, History of electrophoresis, HPCE-based viral titer assay, Immunoelectrophoresis, Iontocaine, Iontophoresis, Isoelectric focusing, Isotachophoresis, Kinetic capillary electrophoresis, Label-free HPCE, Moving-boundary electrophoresis, Patrick H. O'Farrell, Process analytical chemistry, Protomap (proteomics), Pulsed field gel electrophoresis, QPNC-PAGE, SDS-PAGE, Serum protein electrophoresis, Temperature gradient gel electrophoresis, Terminal restriction fragment length polymorphism, Two-dimensional gel electrophoresis.
Author : Mark A. Strege
Publisher : Springer Science & Business Media
ISBN 13 : 159259798X
Total Pages : 340 pages
Book Rating : 4.5/5 (925 download)
Download or read book Capillary Electrophoresis of Proteins and Peptides written by Mark A. Strege and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.
Author : Colin F. Poole
Publisher : Elsevier
ISBN 13 : 0128096144
Total Pages : 628 pages
Book Rating : 4.1/5 (28 download)
Download or read book Capillary Electromigration Separation Methods written by Colin F. Poole and published by Elsevier. This book was released on 2018-04-13 with total page 628 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins. Edited and authored by world-leading capillary electrophoresis experts Presents comprehensive coverage on the subject Includes extensive referencing that facilitates the identification of key research developments Provides more than 50 figures and tables that aid in the retention of key concepts
Author : Andrew Chan
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (13 download)
Download or read book A Method for Selective Concentrating of DNA Targets by Capillary Affinity Gel Electrophoresis written by Andrew Chan and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Robert Weinberger
Publisher : Academic Press
ISBN 13 :
Total Pages : 488 pages
Book Rating : 4.3/5 (91 download)
Download or read book Practical Capillary Electrophoresis written by Robert Weinberger and published by Academic Press. This book was released on 2000-02-16 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary zone electrophoresis : basic concepts -- Capillary zone electrophoresis : methods development -- Capillary zone electrophoresis : secondary equilibrium, micelles, cyclodextrins, and related reagents -- Capillary isoelectric focusing -- Size separations in capillary gels and polymer networks -- Capillary electrochromatography -- Injection -- Detection -- Putting it all together.
Author : James P. Landers
Publisher : CRC Press
ISBN 13 :
Total Pages : 690 pages
Book Rating : 4.3/5 (91 download)
Download or read book Handbook of Capillary Electrophoresis written by James P. Landers and published by CRC Press. This book was released on 1994 with total page 690 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Capillary Electrophoresis is a valuable collection of in-depth discussions of specific areas of capillary electrophoresis (CE) that are key to the practical use of CE for sample analysis. The handbook is divided into five parts. Part I focuses on various modes of CE that have been developed and applied to analytical/separation problems. Part II covers detection in CE, with one section reviewing all detector systems that have been successfully interfaced with CE and another that focuses on CE-mass spectrometry. Part III explores how CE can be applied to the analysis of a specific class of molecules/analytes or to solving separation problems. CE analysis of organic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments; the components of single cells; and the utility of capillary coatings in CE are all covered in this section. A key element of this handbook is its discussion of the potential role of CE for solving clinical problems and the potential for CE and CE-MS to advance analysis of drug metabolism. The handbook also presents practical and theoretical discussions on various aspects of CE, including sample matrix and its effects on separation, the need for controlling capillary temperature, and the present and future implications of EOF control. In-depth discussions of this nature comprise Parts IV and V. The Handbook of Capillary Electrophoresis will be an outstanding reference for analytical chemists, biochemists, clinical chemists, cell biologists, and pharmaceutical researchers.
Author : Paul D. Grossman
Publisher :
ISBN 13 :
Total Pages : 376 pages
Book Rating : 4.:/5 (44 download)
Download or read book Capillary Electrophoresis written by Paul D. Grossman and published by . This book was released on 1992 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations. This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on: Electroosmosis, separation efficiency, and Joule heating Detection methods In-depth discussion of the separation principles and capabilities of the major modes of CE Sieving gel electrophoresis Isoelectric focusing Free solution CE Micellar electrokinetic capillary chromatography Entangled polymer matrix-based separation Detailed treatment of the application of CE to a wide range of molecules, supplemented with extensive "hands-on" illustrations.
Author : Hasan Parvez
Publisher : CRC Press
ISBN 13 : 1466563699
Total Pages : 495 pages
Book Rating : 4.4/5 (665 download)
Download or read book Capillary Electrophoresis in Biotechnology and Environmental Analysis written by Hasan Parvez and published by CRC Press. This book was released on 2023-01-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text aims to evaluate the actual impact of high-performance capillary electrophoresis on analytical biotechnology and environmental analysis. The first part of the book presents a survey of present innovations in instrument design and different methods of pre-concentration techniques in order to obtain increased separations at higher sensitivities. The second part contains articles on applications of HPCE to protein and peptide analysis. In the third part, applications of HPCE in the investigation of drug abuse and drug interactions are presented. The last two parts of the book deal with the use of HPCE at low-UV wavelengths and negative-UV absorption. The book should be of interest to those working in HPCE research and applications.
Author : Reinhard H. H. Neubert
Publisher : CRC Press
ISBN 13 : 9781135546304
Total Pages : 384 pages
Book Rating : 4.5/5 (463 download)
Download or read book Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics written by Reinhard H. H. Neubert and published by CRC Press. This book was released on 2003-01-14 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference presents the most recent breakthroughs and techniques in affinity capillary electrophoresis (ACE) to measure and determine the physicochemical and thermodynamic parameters of drug compounds. The authors offer strategies to explore and characterize interactions between drugs, drug vehicles, and biological membranes to facilitate developments in controlled drug delivery and targeting. The book contains current applications and procedures to identify binding constants between drugs, biological structures, proteins, nucleic acids, and plasmids, increase drug bioavailability, enhance the permeability, solubility, strength, and function of drug formulations, examine protein-protein exchanges and reactions, and calculate pKa values.
Author : Jan Breborowicz
Publisher : CRC Press
ISBN 13 : 1351078003
Total Pages : 293 pages
Book Rating : 4.3/5 (51 download)
Download or read book Affinity Electrophoresis written by Jan Breborowicz and published by CRC Press. This book was released on 2018-01-18 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the topic of affinity electrophoresis (AFF-EP), which has become a useful tool for studies of biomolecular interactions. The book will discuss AFF-EP as an analytical method which has been used successfully for the diagnosis, differentiation, and monitoring of patients with various diseases. The book will also discuss other uses for the AFF-EP method.
Author : H. Shintani
Publisher : Springer Science & Business Media
ISBN 13 : 9400915616
Total Pages : 750 pages
Book Rating : 4.4/5 (9 download)
Download or read book Handbook of Capillary Electrophoresis Applications written by H. Shintani and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.
Author : Jan-Christer Janson
Publisher : John Wiley & Sons
ISBN 13 : 9780471186267
Total Pages : 712 pages
Book Rating : 4.1/5 (862 download)
Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Author : Andrew Chan
Publisher :
ISBN 13 : 9780494160855
Total Pages : 310 pages
Book Rating : 4.1/5 (68 download)
Download or read book Towards the Development and Characterization of a Selective DNA Purification Protocol by Capillary Affinity Gel Electrophoresis written by Andrew Chan and published by . This book was released on 2006 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: A purification protocol based on the selective capture of DNA targets from 'real' samples was developed. The capture material was manufactured by the immobilization of DNA probes into polyacrylamide. The affinity gel was loaded inside capillaries, and samples of single-stranded DNA targets were introduced and eluted by electrophoresis. Characterization of the affinity gels was performed using fluorescently-labelled DNA probes and targets. The affinity gel selectively captured a complementary target, even in the presence of non-complementary and five-base pair mismatch targets. The yield of probe immobilization in the affinity gel was found to be 40%, with capture and elution efficiencies of 80-93% and 81-95%, respectively. Preliminary work on a fraction collection system to directly measure the amount of target eluted was also performed. Characterization of the performance of the affinity capture gel is important to allow for reproducible delivery of a purified sample for subsequent quantification of the target.
Author : Anurag Rathore
Publisher : CRC Press
ISBN 13 : 1135526834
Total Pages : 460 pages
Book Rating : 4.1/5 (355 download)
Download or read book Electrokinetic Phenomena written by Anurag Rathore and published by CRC Press. This book was released on 2003-09-16 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrokinetic Phenomena emphasizes the impact of methods such as capillary zone electrophoresis, capillary electrochromatography, and capillary gel electrophoresis on the analysis of biomolecules. This reference reveals the electrokinetic phenomena that underlie high-performance electro-based analytical tools and vividly depicts how electrodriven analytical tools revolutionize and expedite chemical, pharmaceutical, and biotechnological analysis. An authoritative overview, the book provides effective pathways for large-scale biomedical applications and describes how microfabricated and automated devices enhance and accelerate the analysis of biologically important molecules.
