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Advanced Techniques In Biological Electron Microscopy Specific Ultrastructural Probes
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Book Synopsis Advanced Techniques in Biological Electron Microscopy: Specific ultrastructural probes by : James K. Koehler
Download or read book Advanced Techniques in Biological Electron Microscopy: Specific ultrastructural probes written by James K. Koehler and published by . This book was released on 1973 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advanced Techniques in Biological Electron Microscopy II by : J. K. Koehler
Download or read book Advanced Techniques in Biological Electron Microscopy II written by J. K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of the term "advanced" in the title of this book is somewhat ar bitrary and very much relative with respect to time. Many techniques which were considered at the "cutting edge" of ultrastructural methodology just a few years ago are now rou tin ely used in numerous laboratories. One could cite freeze-fracture, cryothin sectioning, or indeed most of the field of scan ning electron microscopy as concrete examples. Thus the use of the term "ad vanced techniques" must be interpreted with regard to the present state of the art, and is useful only in informing the potential reader that this volume is not a primer to be used as an initial introduction into basic biological elec tron microscopy. Many excellent volumes have filled that niche in the past few years, and it is not intended that this modest book be a complete com pendium of the field. Furthermore, any limited selection of papers on advanc ed techniques necessarily reflects the preferences and arbitrary whims of the editor, thereby excluding many equally important procedures which the knowledgeable reader will readily identify. The first volume of this series appeared approximately five years ago and illustrated techniques which were thought to represent advanced and yet ba sically morphological methods for gaining increased ultrastructural informa tion from biological specimens. The present volume, on the other hand, stresses techniques which provide specific physicochemical data on the speci mens in addition to the structural information.
Book Synopsis Specific Ultrastructural Probes by :
Download or read book Specific Ultrastructural Probes written by and published by . This book was released on 1978 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advanced Techniques in Biological Electron Microscopy III by : J.K. Koehler
Download or read book Advanced Techniques in Biological Electron Microscopy III written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a continuation of two prior books on advanced electron microscope techniques. The purpose of this series has been to provide in depth analyses of methods which are considered to be at the leading edge of electron microscopic research procedures with applications in the biological sciences. The mission of the present volume remains that of a source book for the research practitioner or advanced student, especially one already well versed in basic electron optical methods. It is not meant to provide in troductory material, nor can this modest volume hope to cover the entire spectrum of advanced technology now available in electron microscopy. In the past decade, computers have found their way into many research laboratories thanks to the enormous increase in computing power and stor age available at a modest cost. The ultrastructural area has also benefited from this expansion in a number of ways which will be illustrated in this volume. Half of the contributions discuss technologies that either directly or indirectly make extensive use of computer methods.
Book Synopsis Advanced Techniques in Biological Electron Microscopy by : J.K. Koehler
Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Book Synopsis Advanced Techniques in Biological Electron Microscopy by : James K. Koehler
Download or read book Advanced Techniques in Biological Electron Microscopy written by James K. Koehler and published by Springer. This book was released on 1986 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three friends encounter a terrifying experience with a time warp when one falls into a cupboard and becomes an infant.
Book Synopsis Advanced techniques in biological electron microscopy by : J. K. Koehler
Download or read book Advanced techniques in biological electron microscopy written by J. K. Koehler and published by . This book was released on 1978 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Specific ultrastructral probes by : James K. Koehler
Download or read book Specific ultrastructral probes written by James K. Koehler and published by . This book was released on 1973 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biological Electron Microscopy by : Michael J. Dykstra
Download or read book Biological Electron Microscopy written by Michael J. Dykstra and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.
Book Synopsis Advanced Techniques in Biological Electron Microscopy by : James K. Koehler
Download or read book Advanced Techniques in Biological Electron Microscopy written by James K. Koehler and published by . This book was released on 1973 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advanced Techniques in Biological Electron Microscopy: without special title by : James K. Koehler
Download or read book Advanced Techniques in Biological Electron Microscopy: without special title written by James K. Koehler and published by . This book was released on 1973 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :M. A. Nasser Hajibagheri Publisher :Springer Science & Business Media ISBN 13 :1592592015 Total Pages :292 pages Book Rating :4.5/5 (925 download)
Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri
Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Book Synopsis Electron Probe Microanalysis by : Karl Zierold
Download or read book Electron Probe Microanalysis written by Karl Zierold and published by Springer Science & Business Media. This book was released on 2013-03-08 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.
Download or read book Volume Electron Microscopy written by and published by Elsevier. This book was released on 2023-07-13 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume Electron Microscopy (vEM), Volume 177 is a collective term for a set of three-dimensional high-resolution ultrastructural imaging techniques that have delivered new insights into biological systems in recent years, garnering substantial interest in the life and clinical sciences. In this book, users will find a variety of vEM workflows and technologies, highlighting application areas with biologically relevant examples. Topics covered include Automated large volume sample preparation for vEM, Resin comparison for Serial Block Face Scanning Volume Electron Microscopy, Immunolabelling for SBF-SEM, Electron Microscopy in Plants, Serial section electron tomography, Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology, Array tomography, and much more. Other sections focus on Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy, Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea, Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue, Methods of enhanced FIB-SEM sample preparation and image acquisition, Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy, and much more. Includes chapters written by key leaders and developers in the field Provides detailed protocols, allowing for the application of workflows in one’s own laboratory setting Presents real tips and tricks you won't get from standard research papers
Book Synopsis In Situ Hybridization in Electron Microscopy by : Gerard Morel
Download or read book In Situ Hybridization in Electron Microscopy written by Gerard Morel and published by CRC Press. This book was released on 2001-01-30 with total page 590 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization
Book Synopsis Correlative Light and Electron Microscopy IV by :
Download or read book Correlative Light and Electron Microscopy IV written by and published by Academic Press. This book was released on 2021-03-09 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more. Contains contributions from experts in the field Covers topics using nano-SIMS and EDX for CLEM Presents recent advances and currently applied correlative approaches Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting Covers CLEM approaches in the context of specific applications Aims to stimulate the use of new combinations of imaging modalities
Book Synopsis Electron Microscopy by : John J. Bozzola
Download or read book Electron Microscopy written by John J. Bozzola and published by Jones & Bartlett Learning. This book was released on 1999 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR
