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Virus Separation And Purification Methods
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Book Synopsis Virus Separation and Purification Methods by : Alfred Polson
Download or read book Virus Separation and Purification Methods written by Alfred Polson and published by CRC Press. This book was released on 1993-07-29 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference presents many contributions made by Dr Alfred Polson during his 41 years of research into the physicochemical properties of plant and animal viruses - detailing his timesaving approaches to the characterization, extraction, separation, concentration and purification of viruses, proteins, antibodies and biopolymers.;Describing successful laboratory techniques featuring the Beckman preparative centrifuge, this reference: examines the electro-extraction process for isolating the maximum amount of viruses from infected plant material; analyzes zone electrophoresis in sugar concentration gradients as a powerful tool for purifying entero-, insect born and insect viruses; contains material on the use of a reorienting gradient centrifuge rotor to separate components of the haemolymph of the mollusc Turbo sarmaticus as a model for virus separation; explains the construction of a modified thin-layer ultracentrifuge rotor for concentrating viruses into suspension rather than pellets; elucidates the use of inserts in ultracentrifuge tubes in order to decrease the time of centrifuging; and introduces a method for determining pure viruse suspension densities.;This resource is intended for microbiologists, virologists, biochemists, molecular and cell biologists, immunologists, biochemical and bioprocess engineers, chemical engineers and graduate students in these disciplines.
Book Synopsis Special Issue on Virus Separation and Purification Methods by : Alfred Polson
Download or read book Special Issue on Virus Separation and Purification Methods written by Alfred Polson and published by . This book was released on 1993 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods in Virology by : Karl Maramorosch
Download or read book Methods in Virology written by Karl Maramorosch and published by . This book was released on 1967 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ultracentrifuge; Equilibrium ultracentrifugation; Density-gradient centrifugation; Miscellaneous problems in virus purification; New centrifugal methods for virus isolation; Chromatography and membrane separation; Water-organic solvent phase systems; Virus concentration by ultrafiltration; Diffusion; Two-phase separation of viruses; Purification of virus by adsorption on cells and elution; Molecular sieve methods; Filtration techniques; Electrophoresis of viruses; Labeling of viruses with isotopes; Separation of viruses into components; Methods of degrading nucleic acids and separating the components; Assay of infectivity of infectivity of nucleic acids.
Book Synopsis Molecular Virology of Human Pathogenic Viruses by : Wang-Shick Ryu
Download or read book Molecular Virology of Human Pathogenic Viruses written by Wang-Shick Ryu and published by Academic Press. This book was released on 2016-03-30 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Virology of Human Pathogenic Viruses presents robust coverage of the key principles of molecular virology while emphasizing virus family structure and providing key context points for topical advances in the field. The book is organized in a logical manner to aid in student discoverability and comprehension and is based on the author’s more than 20 years of teaching experience. Each chapter will describe the viral life cycle covering the order of classification, virion and genome structure, viral proteins, life cycle, and the effect on host and an emphasis on virus-host interaction is conveyed throughout the text. Molecular Virology of Human Pathogenic Viruses provides essential information for students and professionals in virology, molecular biology, microbiology, infectious disease, and immunology and contains outstanding features such as study questions and recommended journal articles with perspectives at the end of each chapter to assist students with scientific inquiries and in reading primary literature. Presents viruses within their family structure Contains recommended journal articles with perspectives to put primary literature in context Includes integrated recommended reading references within each chapter Provides access to online ancillary package inclusive of annotated PowerPoint images, instructor’s manual, study guide, and test bank
Book Synopsis Introduction to Virology by : K. Smith
Download or read book Introduction to Virology written by K. Smith and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of viruses, or virology as it is now called, had its origin in 1892 when a Russian botanist, Iwanawsky, showed that sap from a tobacco plant with an infectious disease was still highly infectious after passage through a filter capable of retaining bacterial cells. From such humble beginnings the study of these 'filter-passing agents', or viruses, has developed into a separate science which rivals, if it does not excel, in importance the whole of bacteriology. The importance of viruses lies not only in the diseases they cause in every type of living organism, but also because of their intimate relationship with the living cell, in which alone they can reproduce. Their study has influenced the whole of biology by greatly increasing our knowledge of the gene, genetics, and molecular structure; there is also the possible connexion of viruses with human cancer, in view of the occurrence of many viral cancers in other animals. The book attempts to give a comprehensive but necessarily superficial survey of the subject as a whole and should help senior undergraduates and postgraduate students who wish to gain some knowledge of virology. Further information is available from the extensive bibliography.
Book Synopsis Viral Ecology by : Christon J. Hurst
Download or read book Viral Ecology written by Christon J. Hurst and published by Academic Press. This book was released on 2000-04-10 with total page 670 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viral Ecology defines and explains the ecology of viruses by examining their interactions with their hosting species, including the types of transmission cycles that have evolved, encompassing principal and alternate hosts, vehicles, and vectors. It examines virology from an organismal biology approach, focusing on the concept that viral infections represent areas of overlap in the ecology of viruses, their hosts, and their vectors. The relationship between viruses and their hosting species The concept that viral interactions with their hosts represents a highly evolved aspect of organismal biology The types of transmission cycles which exist for viruses, including their hosts, vectors, and vehicles The concept that viral infections represent areas of overlap in the ecology of the viruses, their hosts, and their vectors
Book Synopsis Vaccine Development and Manufacturing by : Emily P. Wen
Download or read book Vaccine Development and Manufacturing written by Emily P. Wen and published by John Wiley & Sons. This book was released on 2014-10-06 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vaccine Manufacturing and Production is an invaluable reference on how to produce a vaccine - from beginning to end - addressing all classes of vaccines from a processing, production, and regulatory viewpoint. It will provide comprehensive information on the various fields involved in the production of vaccines, from fermentation, purification, formulation, to regulatory filing and facility designs. In recent years, there have been tremendous advances in all aspects of vaccine manufacturing. Improved technology and growth media have been developed for the production of cell culture with high cell density or fermentation. Vaccine Manufacturing and Production will serve as a reference on all aspects of vaccine production by providing an in-depth description of the available technologies for making different types of vaccines and the current thinking in facility designs and supply issues. This book will provide insight to the issues scientists face when producing a vaccine, the steps that are involved, and will serve as a reference tool regarding state-of-the-art vaccine manufacturing technologies and facility set-up. Highlights include: Comprehensive coverage of vaccine production : from a process point of view- fermentation to purification to formulation developments; from a production point of view - from facility design to manufacturing; and from a regulatory point of view - requirements from government agencies Authors from different major pharmaceutical and biotechnology companies Describes the challenges and issues involved in vaccine production and manufacturing of the different classes of vaccines, an area not covered by other books currently on the market
Book Synopsis Viruses: Essential Agents of Life by : Günther Witzany
Download or read book Viruses: Essential Agents of Life written by Günther Witzany and published by Springer Science & Business Media. This book was released on 2012-11-13 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: A renaissance of virus research is taking centre stage in biology. Empirical data from the last decade indicate the important roles of viruses, both in the evolution of all life and as symbionts of host organisms. There is increasing evidence that all cellular life is colonized by exogenous and/or endogenous viruses in a non-lytic but persistent lifestyle. Viruses and viral parts form the most numerous genetic matter on this planet.
Book Synopsis Process Scale Purification of Antibodies by : Uwe Gottschalk
Download or read book Process Scale Purification of Antibodies written by Uwe Gottschalk and published by John Wiley & Sons. This book was released on 2017-03-07 with total page 849 pages. Available in PDF, EPUB and Kindle. Book excerpt: Promoting a continued and much-needed renaissance in biopharmaceutical manufacturing, this book covers the different strategies and assembles top-tier technology experts to address the challenges of antibody purification. • Updates existing topics and adds new ones that include purification of antibodies produced in novel production systems, novel separation technologies, novel antibody formats and alternative scaffolds, and strategies for ton-scale manufacturing • Presents new and updated discussions of different purification technologies, focusing on how they can address the capacity crunch in antibody purification • Emphasizes antibodies and innovative chromatography methods for processing
Book Synopsis Process Chromatography by : Gail K. Sofer
Download or read book Process Chromatography written by Gail K. Sofer and published by Academic Press. This book was released on 2015-09-02 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research and development into biological products for therapeutic use has increased dramatically over the last 10 years. With this, strict regulatory requirements have been imposed by authorities such as the U.S. Food & Drug Administration, so that today validation has become a key issue in the biopharmaceutical industry.This concise book addresses validation issues in the chromatography of biotherapeutics. It covers process design, qualification and validation, including an overview of analytical techniques commonly used in the validation of processes. A concluding section comments on product changeover and presents four case studies.
Book Synopsis Virology Methods Manual by : Hillar O. Kangro
Download or read book Virology Methods Manual written by Hillar O. Kangro and published by Elsevier. This book was released on 1996-04-16 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work! Provides up-to-date techniques by experts worldwide Presents common, step-by-step protocols in an attractive, easy-to-use fashion Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory
Book Synopsis Stationary and Mobile Phase Selection for Ion-exchange Chromatography of Viruses by : Egor Trilisky
Download or read book Stationary and Mobile Phase Selection for Ion-exchange Chromatography of Viruses written by Egor Trilisky and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Purified viruses can serve as gene delivery vectors and are the active ingredients in many vaccines. Additionally, for purification of therapeutic proteins, not only must the separation steps be capable of removing viruses, the regulatory agencies (FDA, EMEA, etc.) also require understanding of the removal mechanisms to ensure robustness. Ion-exchange chromatography (IEC) is the most widely used method for protein purification and it has been demonstrated to be more efficient for virus purification than density gradient ultracentrifugation, the traditional method for virus purification. Chromatography of viruses has been considered to be a black box and designing IEC processes is empirical and time-consuming. The goal of this work was to improve the understanding and process development of virus IEC. We developed light scattering and batch binding techniques for rapid optimization of pH and ionic strength. The model virus was human adenovirus type 5 (Ad5), the most commonly used virus in gene therapy. Ad5 was found to aggregate within about 1.5-2 pH units of its isoelectric point. Due to strong repulsion between virus particles at low ionic strengths, binding of Ad5 to a model stationary phase attains a maximum with increasing ionic strength. Besides pH and ionic strength, choice of a stationary phase is another key variable in IEC. In addition to typical resins used for protein purification, which have pores that are smaller than most viruses, we evaluated perfusive resins and monoliths by single-component pulse and step response studies. The evaluation was performed with small molecules, proteins, and the virus to examine how increasing bioparticle size affects chromatographic behavior, and the results were related to stationary phase structural parameters. Binding capacities on a model monolith were predicted from the pore size distribution. Modeling of breakthrough curves on a monolith showed that the experimentally observed slow approach to full saturation is due to the distribution of pore sizes rather than diffusive limitations. For larger bioparticles, retention is generally greater and the transition from a non-eluting to a non-binding state with increasing ionic strength is more abrupt. The drag imposed on adsorbed bioparticles by the moving liquid, which may contribute to desorption, is calculated to be more important for larger analytes. With increasing retention, the height equivalent to a theoretical plate increased significantly more for larger bioparticles. For proper analysis of peak data, an algorithm was developed to account for temporal distortion and extra-column effects. The skew caused by temporal distortion was found to be inversely related to the number of theoretical plates and, therefore, bed height. Under loading conditions, Ad5 is unable to enter the internal pore space of perfusive resins because virus particles that bind near the external surface prevent others from entering the pores. A more significant issue was observed for both perfusive resins and monoliths: even under non-binding conditions, with increasing flow rate, a progressively larger fraction of virus particles injected onto the columns does not elute. Calculations show that the cause is convective entrapment of virus particles in pores with constrictions. As expected from the developed theory, the entrapment is reversible upon flow rate reduction and does not occur on small-pore resins, nor for proteins. This finding represents a new retention mechanism in chromatography. The theory has multiple implications for chromatography of large biologics on perfusive resins, monoliths, and membranes in terms of product loss, underestimation of analyte concentration, and column cleaning. Entrapment can be controlled by adjusting the flow rate.
Book Synopsis Characterization of Plant Viruses by : Alangar Ishwara Bhat
Download or read book Characterization of Plant Viruses written by Alangar Ishwara Bhat and published by Humana. This book was released on 2021-03-20 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.
Author :Richard Ellis Ford Matthews Publisher :Gulf Professional Publishing ISBN 13 :9780123611604 Total Pages :1084 pages Book Rating :4.6/5 (116 download)
Book Synopsis Matthews' Plant Virology by : Richard Ellis Ford Matthews
Download or read book Matthews' Plant Virology written by Richard Ellis Ford Matthews and published by Gulf Professional Publishing. This book was released on 2002 with total page 1084 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been ten years since the publication of the third edition of this seminal text on plant virology, during which there has been an explosion of conceptual and factual advances. The fourth edition updates and revises many details of the previous editon, while retaining the important older results that constitute the field's conceptual foundation. Key features of the fourth edition include: * Thumbnail sketches of each genera and family groups * Genome maps of all genera for which they are known * Genetic engineered resistance strategies for virus disease control * Latest understanding of virus interactions with plants, including gene silencing * Interactions between viruses and insect, fungal, and nematode vectors * New plate section containing over 50 full-color illustrations.
Book Synopsis Viral Vectors for Gene Therapy by : Otto-Wilhelm Merten
Download or read book Viral Vectors for Gene Therapy written by Otto-Wilhelm Merten and published by Humana. This book was released on 2011-05-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection. In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures. Written in the highly successful Methods in Molecular BiologyTM series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.
Download or read book Virus filtration written by Kurt Brorson and published by . This book was released on 2008 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applied Plant Virology by : L. P. Awasthi
Download or read book Applied Plant Virology written by L. P. Awasthi and published by Academic Press. This book was released on 2020-05-14 with total page 851 pages. Available in PDF, EPUB and Kindle. Book excerpt: Applied Plant Virology: Advances, Detection, and Antiviral Strategies provides an overview on recent developments and applications in the field of plant virology. The book begins with an introduction to important advances in plant virology, but then covers topics including techniques for assay detection and the diagnosis of plant viruses, the purification, isolation and characterization of plant viruses, the architecture of plant viruses, the replication of plant viruses, the physiology of virus-infected hosts, vectors of plant viruses, and the nomenclature and classification of plants. The book also discusses defense strategies by utilizing antiviral agents and management strategies of virus and viroid diseases. With contributions from an international collection of experts, this book presents a practical resource for plant virologists, plant pathologists, horticulturalists, agronomists, biotechnologists, academics and researchers interested in up-to-date technologies and information that advance the field of plant virology. Covers the detection, control and management of plant viruses Discusses antiviral strategies, along with mechanisms of systemic induced resistance to enhance the defense of plants against viruses Provides contributory chapters from expert plant virologists from different parts of the world
