Author : Sushil Kumar
Publisher :
ISBN 13 :
Total Pages : 238 pages
Book Rating : 4.:/5 (89 download)
Book Synopsis Tuberization in Vitro and Physiological Studies with Potato Callus Tissues by : Sushil Kumar
Download or read book Tuberization in Vitro and Physiological Studies with Potato Callus Tissues written by Sushil Kumar and published by . This book was released on 1963 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Potato plants were grown from stem sections, sprouts, seeds and embryos in vitro on nutrient media. Growth was compared on T-medium, a basal mineral salts-sucrose medium; or C-medium, which is T-medium with coconut milk (CM), or naphthalenacetic acid (NAA) and calcium pantothenate; or D-medium, which is C-medium with 2,4-dichlorophenoxyacetic acid (2,4-D). In all cases D-medium induced only callus formation and failed to promote roots and stems. Basal T-medium induced plant growth without callus formation but growth of plants, was not as fast or as good as that induced by C-medium. The C-medium induced stem sections, sprouts and seeds to grow directly into plants. Seventy-five per cent of embryos on C-medium grew directly into seedling but the other 25% of embryos formed only callus. However, these callus tissues left long enought on C-medium usually developed leaves and roots. Effects of daylight and temperature were studied, probably for the first time, on growth and tuberization in potato plants that were grown in vitro. Sterile cultures of plants in vitro coming from different sources and variates of potato when incubated for eight hours of light in the 16o, 20o and 24oC greenhouses and for 16 hours in the dark at low night temperature were benefited in growth and tuberization. The low temperature of 16o C stimulated better growth and tuberization than the 20o and 24o C temperatures. The increase in temperature reduced growth and tuberization. It was, therefore, possible to increase or decrease growth and ...