Time Resolved Fluorescence Spectroscopy In Biochemistry And Biology

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Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology

Author : R. Cundall
Publisher : Springer Science & Business Media
ISBN 13 : 1475716346
Total Pages : 785 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology by : R. Cundall

Download or read book Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology written by R. Cundall and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 785 pages. Available in PDF, EPUB and Kindle. Book excerpt: At the time that the editors conceived the idea of trying to organize the meeting on which the contents of this volume are based and which became, in March 1980, a NATO Advanced Study Institute, the techniques of time-resolved fluorescence spectroscopy, in both the nanosecond and sub-nanosecond time-domains, might reasonably have been said to be coming of age, both in their execution and in the analysis and interpretation of the results obtained. These techniques, then as now, comprised mainly a number of pulse methods using laser, flash-lamp or, most recently, synchrotron radiation. In addition, significant developments in the more classical phase approach had also rendered that method popular, utilizing either modulation of an otherwise continuous source or, again recently, the ultra-rapid pulse rate attainable with a synchrotron source. In general terms, time-resolved fluorescence studies are capable, under appropriate conditions, of supplying direct kinetic information on both photophysics and various aspects of molecular, macromolecular and supramolecular structure and dynamics. The nanosecond and sub-nanosecond time-scales directly probed render these techniques particularly appropriate in studying relaxation and fluctuation processes in macromolecules, particularly biopolymers (e. g. proteins, nucleic acids), in supramolecular assemblies such as cell membranes, and in a variety of relatively simpler model systems.

Fluorescence Spectroscopy in Biology

Author : Martin Hof
Publisher : Springer Science & Business Media
ISBN 13 : 3540270043
Total Pages : 315 pages
Book Rating : 4.5/5 (42 download)

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Book Synopsis Fluorescence Spectroscopy in Biology by : Martin Hof

Download or read book Fluorescence Spectroscopy in Biology written by Martin Hof and published by Springer Science & Business Media. This book was released on 2006-01-27 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Topics in Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0306470608
Total Pages : 502 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Topics in Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Topics in Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Applied Fluorescence in Chemistry, Biology and Medicine

Author : Wolfgang Rettig
Publisher : Springer Science & Business Media
ISBN 13 : 3642599036
Total Pages : 567 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Applied Fluorescence in Chemistry, Biology and Medicine by : Wolfgang Rettig

Download or read book Applied Fluorescence in Chemistry, Biology and Medicine written by Wolfgang Rettig and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: This interdisciplinary book gives a comprehensive survey of the state-of-the-art: from applications and trends in fluorescence techniques in science to medicine and engineering. Written for practitioners and researchers in industry and academia, it covers fields like environmental and materials science, biology, medicine, physics and chemistry. Moreover, it reports on such new and breathtaking methods as ultra-fast time-resolved or single molecule spectroscopy, gives examples of applications in the fields of electroluminescent polymers, visualization of membrane potentials in neurons and fluorescence imaging of the brain.

Time-resolved Laser Spectroscopy in Biochemistry III

Author : Joseph R. Lakowicz
Publisher :
ISBN 13 :
Total Pages : 834 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Time-resolved Laser Spectroscopy in Biochemistry III by : Joseph R. Lakowicz

Download or read book Time-resolved Laser Spectroscopy in Biochemistry III written by Joseph R. Lakowicz and published by . This book was released on 1992 with total page 834 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Principles of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0387463127
Total Pages : 954 pages
Book Rating : 4.3/5 (874 download)

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Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2007-12-05 with total page 954 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Fluorescence Spectroscopy and Microscopy

Author : Yves Engelborghs
Publisher : Humana
ISBN 13 : 9781493963157
Total Pages : 0 pages
Book Rating : 4.9/5 (631 download)

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Book Synopsis Fluorescence Spectroscopy and Microscopy by : Yves Engelborghs

Download or read book Fluorescence Spectroscopy and Microscopy written by Yves Engelborghs and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Nonradioactive Analysis of Biomolecules

Author : Christoph Kessler
Publisher : Springer Science & Business Media
ISBN 13 : 9783540646013
Total Pages : 1170 pages
Book Rating : 4.6/5 (46 download)

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Book Synopsis Nonradioactive Analysis of Biomolecules by : Christoph Kessler

Download or read book Nonradioactive Analysis of Biomolecules written by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2000-03-11 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.

Biochemical Applications

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0306470594
Total Pages : 390 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Biochemical Applications by : Joseph R. Lakowicz

Download or read book Biochemical Applications written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial three volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. These first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).

Fluorescence Spectroscopy and Microscopy in Biology

Author : Radek Šachl
Publisher : Springer Nature
ISBN 13 : 3031303628
Total Pages : 529 pages
Book Rating : 4.0/5 (313 download)

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Book Synopsis Fluorescence Spectroscopy and Microscopy in Biology by : Radek Šachl

Download or read book Fluorescence Spectroscopy and Microscopy in Biology written by Radek Šachl and published by Springer Nature. This book was released on 2023-04-27 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.

Perspectives on Fluorescence

Author : David M. Jameson
Publisher : Springer
ISBN 13 : 3319413287
Total Pages : 346 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Perspectives on Fluorescence by : David M. Jameson

Download or read book Perspectives on Fluorescence written by David M. Jameson and published by Springer. This book was released on 2016-08-08 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gregorio Weber is widely acknowledged as the person responsible for the advent of modern fluorescence spectroscopy. Since 2016 is the 100th anniversary of Gregorio Weber’s birth, this special volume has been prepared to honor his life and achievements. It offers contributions from outstanding researchers in the fluorescence field, describing their perspectives on modern fluorescence and its highly diverse applications, ranging from the photophysics of tryptophan and proteins, membrane studies, fluorescence microscopy on live cells, novel software approaches and instrumentation. Many of the authors knew Gregorio Weber personally and have shared their impressions of the man and his contributions. This volume appeals not only to aficionados of fluorescence spectroscopy and its applications in biology, chemistry and physics, but also to those with a general interest in the historical development of an important scientific field.

Biophysical and Biochemical Aspects of Fluorescence Spectroscopy

Author : T.G. Dewey
Publisher : Springer Science & Business Media
ISBN 13 : 1475795130
Total Pages : 305 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Biophysical and Biochemical Aspects of Fluorescence Spectroscopy by : T.G. Dewey

Download or read book Biophysical and Biochemical Aspects of Fluorescence Spectroscopy written by T.G. Dewey and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy has traditionally found wide application in bio chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex ploited with great success utilizing both intrinsic and extrinsic probes. Re cent applications have built upon these traditional strengths and have re sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of fundamental processes. Frequently, fluorescence techniques are tailored to attack a specific biological problem. These new methods in turn produce new physical situations and phenomena which are often of interest to the physical chemist. Thus, progress in one area stimulates renewed interest in other areas. The goal of this book is to provide detailed monographs on the use of fluorescence to investigate problems at the forefront of biochemistry and cell biology. This book is not meant to be a comprehensive survey but rather to highlight areas of recent developments. It is designed to be readable to the novice and yet provide sufficient detail for the expert to keep abreast of recent developments. The book is organized so that it proceeds from simple biochemical sys tems to more complex cell biological ones. Chapter I on fluorescence quenching of biological structures is a good introductory chapter. It intro duces a number of elementary concepts and discusses applications to pro teins and biomembranes.

Principles of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 146157658X
Total Pages : 487 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

Fluorescent Biomolecules

Author : David M. Jameson
Publisher : Springer Science & Business Media
ISBN 13 : 1468456199
Total Pages : 459 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Fluorescent Biomolecules by : David M. Jameson

Download or read book Fluorescent Biomolecules written by David M. Jameson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is based on an international symposium held during September 9-12, 1986 in Bocca di Magra, Italy. The intent of the organizers was to bring together expert practitioners of fluorescence spectroscopy, particularly as applied to biological systems, to assess recent developments in the field and discuss future directions. At the same time the meeting was intended to honor the singular and outstanding scientific career of Gregorio Weber on the occasion of his seventieth birthday. Gregorio Weber is truly the pioneer in the application of fluores cence methods to biochemistry and biophysics. A complete list of his scientific contributions to fluorescence and to protein biochemistry is beyond the scope of this preface. Suffice it to say that since his initial landmark articles on fluorescence, published in the late 1940's and early 1950's, Gregorio Weber has continued to make seminal contribu tions to both the theory and practice of fluorescence and, contrary to many who might be tempted to rest on their laurels, he shows no signs of slackening his pace. In addition to his more obvious tangible contributions to the scientific field, Gregorio Weber has made equally valuable contributions of another type. Specifically, he has had the most profound impact, both profeSSionally and personally, on generations of young scientists.

Who’s Who in Fluorescence 2004

Author : Chris D. Geddes
Publisher : Springer Science & Business Media
ISBN 13 : 1441991506
Total Pages : 216 pages
Book Rating : 4.4/5 (419 download)

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Book Synopsis Who’s Who in Fluorescence 2004 by : Chris D. Geddes

Download or read book Who’s Who in Fluorescence 2004 written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Who's Who in Fluorescence 2003 volume was published in November 2002. It featured some 312 personal entries from fluorescence workers all over the world. Initially we were unsure how useful the volume would be. However, it wasn't very long before we were inundated with requests for both bulk and personal orders. In addition a significant number of copies were freely distributed at conference venues, such as at the Biophysical Society meeting in San Antonio. Texas, March 2003, and at the Methods and Applications of Fluorescence Spectroscopy conference (MAFS) in Prague, Czech Republic, August 2003, where these two venues probably host the largest gathering of Fluorescence workers anywhere. Even when we were initially taking e-mail based submissions, contributors were freely commenting on what a useful resource they saw the volume as being. We subsequently shared these comments on the back outside cover of the 2003 volume. As well as individual scientists supporting the 2003 volume, the Fluorescence based Companies also played a key role, where without their financial support, the volume probably would not have the impact it currently has. As such, the Who's Who in Fluorescence 2003 has been a much bigger success than we ever envisaged. Subsequently, we now present the Who's Who in Fluorescence 2004 volume. The new volume features 359 personal entries from 35 countries around the world. In addition we have increased company support, which should enable us to distribute more copies at targeted venues in 2004.

Spectroscopy In Biochemistry

Author : J.Ellis Bell
Publisher : CRC Press
ISBN 13 : 135109372X
Total Pages : 394 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Spectroscopy In Biochemistry by : J.Ellis Bell

Download or read book Spectroscopy In Biochemistry written by J.Ellis Bell and published by CRC Press. This book was released on 2018-05-04 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been written in part with the aim of providing a text which will be useful in teaching the biochemical applications of spectroscopy. This book will be of particular use to the biochemist or biologist who does not have a background in spectroscopy, but desires to find out what sort of information spectroscopy can provide. Attention was limited to those techniques most frequently used, and which at present have the widest applications.

Topics in Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0306470586
Total Pages : 432 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Topics in Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Topics in Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial two volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. The third volume will appear shortly. The first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).