The Use of Mass Spectrometry to Characterize the Metabolic Output of Bacterial Pathogens

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Publisher :
ISBN 13 : 9781124554891
Total Pages : 172 pages
Book Rating : 4.5/5 (548 download)

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Book Synopsis The Use of Mass Spectrometry to Characterize the Metabolic Output of Bacterial Pathogens by : David Junior Gonzalez

Download or read book The Use of Mass Spectrometry to Characterize the Metabolic Output of Bacterial Pathogens written by David Junior Gonzalez and published by . This book was released on 2011 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overall theme of this thesis is the use of mass spectrometry to answer questions of biological relevance. The thesis begins by informing the reader on the basics of mass spectrometry and associated tools with an introductory chapter. Chapter 2 describes collaborative work performed between three labs at UCSD, the Jack E. Dixon, Victor Nizet and Pieter C. Dorrestein labs. The project was initiated by the use of comparative genomics to discover a widely distribution toxin biosynthetic gene cluster. Molecular and biochemical analysis of a two members of the conserved family, namely Group A Streptococcus and Clostridium Botulinum, demonstrated the organisms indeed contained similar cellular machinery for the biosynthesis of ribosomally encoded peptides putatively containing thiazole and (methyl)-oxazole moieties. Chapter 3 continues characterizing the widely distributed family of toxins specifically characterizing the gene cluster within the bacterium C. Botulinum. The work describes the use of mass spectrometry to obtain structural evidence, verifying the biosynthetic gene cluster indeed produces heterocycles as hypothesized by the genomic studies. Additionally, the work uses biochemical and genetic approaches to characterize a novel [Beta]-hemolytic, cytolytic toxin in two Clostridia species. Chapter 4 introduces the method of imaging mass spectrometry (IMS) of microbial colonies. Although IMS is not a novel technique, very few reports had used the technology to investigate the microbial kingdom. The work within chapter 4 describes an IMS survey performed on a vast number of differential microbes to visualize their associated metabolic output. The goal was to show that IMS is an amenable technique that can be applied to a diverse number of microbes. Chapter 5 goes beyond the proposal set forth in chapter 4, as IMS was used to visualize the interaction between two well characterized model bacterial systems, the probiotic bacterium Bacillus subtilis and the bona fide human pathogen methicillin resistant Staphylococcus aureus (MRSA). Imaging mass spectrometry showed directional release of compounds by the probiotic organism B. subtilis that have antibiotic effects against MRSA giving insight into the potential mode of action for probiotic organisms.

Applications of Mass Spectrometry in Microbiology

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Publisher : Springer
ISBN 13 : 3319260707
Total Pages : 336 pages
Book Rating : 4.3/5 (192 download)

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Book Synopsis Applications of Mass Spectrometry in Microbiology by : Plamen Demirev

Download or read book Applications of Mass Spectrometry in Microbiology written by Plamen Demirev and published by Springer. This book was released on 2016-01-12 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last quarter century, advances in mass spectrometry (MS) have been at the forefront of efforts to map complex biological systems including the human metabolome, proteome, and microbiome. All of these developments have allowed MS to become a well-established molecular level technology for microorganism characterization. MS has demonstrated its considerable advantage as a rapid, accurate, and cost-effective method for microorganism identification, compared to conventional phenotypic techniques. In the last several years, applications of MS for microorganism characterization in research, clinical microbiology, counter-bioterrorism, food safety, and environmental monitoring have been documented in thousands of publications. Regulatory bodies in Europe, the US, and elsewhere have approved MS-based assays for infectious disease diagnostics. As of mid-2015, more than 3300 commercial MS systems for microorganism identification have been deployed worldwide in hospitals and clinical labs. While previous work has covered broader approaches in using MS to characterize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In twelve individual chapters, innovators, leaders and practitioners in the field from around the world have contributed to a comprehensive overview of current and next-generation approaches for MS-based microbial characterization at the subspecies and strain levels. Chapters include up-to-date reference lists as well as web-links to databases, recommended software, and other useful tools. The emergence of new, antibiotic-resistant strains of human or animal pathogens is of extraordinary concern not only to the scientific and medical communities, but to the general public as well. Developments of novel MS-based assays for rapid identification of strains of antibiotic-resistant microorganisms are reviewed in the book as well. Microbiologists, bioanalytical scientists, infectious disease specialists, clinical laboratory and public health practitioners as well as researchers in universities, hospitals, government labs, and the pharmaceutical and biotechnology industries will find this book to be a timely and valuable resource.

Metabolic Profiling

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Publisher : Humana
ISBN 13 : 9781493992522
Total Pages : 291 pages
Book Rating : 4.9/5 (925 download)

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Book Synopsis Metabolic Profiling by : Georgios A. Theodoridis

Download or read book Metabolic Profiling written by Georgios A. Theodoridis and published by Humana. This book was released on 2019-04-30 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.

MALDI-TOF and Tandem MS for Clinical Microbiology

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Publisher : John Wiley & Sons
ISBN 13 : 1118960238
Total Pages : 850 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis MALDI-TOF and Tandem MS for Clinical Microbiology by : Haroun N. Shah

Download or read book MALDI-TOF and Tandem MS for Clinical Microbiology written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2017-03-31 with total page 850 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade. This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss: Challenges of Identifying Mycobacterium to the Species level Identification of Bacteroides and Other Clinically Relevant Anaerobes Identification of Species in Mixed Microbial Populations Detection of Resistance Mechanisms Proteomics as a biomarker discovery and validation platform Determination of Antimicrobial Resistance using Tandem Mass Spectrometry

Comprehensive Natural Products III

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Publisher : Elsevier
ISBN 13 : 0081026919
Total Pages : 4266 pages
Book Rating : 4.0/5 (81 download)

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Book Synopsis Comprehensive Natural Products III by :

Download or read book Comprehensive Natural Products III written by and published by Elsevier. This book was released on 2020-07-22 with total page 4266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive Natural Products III, Third Edition, Seven Volume Set updates and complements the previous two editions, including recent advances in cofactor chemistry, structural diversity of natural products and secondary metabolites, enzymes and enzyme mechanisms and new bioinformatics tools. Natural products research is a dynamic discipline at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids and enzymes. This book reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine and to stimulate new ideas among the established natural products community. Provides readers with an in-depth review of current natural products research and a critical insight into the future direction of the field Bridges the gap in knowledge by covering developments in the field since the second edition published in 2010 Split into 7 sections on key topics to allow students, researchers and professionals to find relevant information quickly and easily Ensures that the knowledge within is easily understood by and applicable to a large audience

Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry

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Publisher : Springer
ISBN 13 : 3319048198
Total Pages : 287 pages
Book Rating : 4.3/5 (19 download)

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Book Synopsis Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry by : Rainer Cramer

Download or read book Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry written by Rainer Cramer and published by Springer. This book was released on 2015-11-09 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics

Plant Metabolomics

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Publisher : Springer Science & Business Media
ISBN 13 : 3540297820
Total Pages : 351 pages
Book Rating : 4.5/5 (42 download)

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Book Synopsis Plant Metabolomics by : Kazuki Saito

Download or read book Plant Metabolomics written by Kazuki Saito and published by Springer Science & Business Media. This book was released on 2006-06-29 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics – which deals with all metabolites of an organism – is a rapidly-emerging sector of post-genome research fields. It plays significant roles in a variety of fields from medicine to agriculture and holds a fundamental position in functional genomics studies and their application in plant biotechnology. This volume comprehensively covers plant metabolomics for the first time. The chapters offer cutting-edge information on analytical technology, bioinformatics and applications. They were all written by leading researchers who have been directly involved in plant metabolomics research throughout the world. Up-to-date information and future developments are described, thereby producing a volume which is a landmark of plant metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all plant science fields.

Proteomics of Microbial Pathogens

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Publisher : John Wiley & Sons
ISBN 13 : 9783527317592
Total Pages : 352 pages
Book Rating : 4.3/5 (175 download)

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Book Synopsis Proteomics of Microbial Pathogens by : Peter R. Jungblut

Download or read book Proteomics of Microbial Pathogens written by Peter R. Jungblut and published by John Wiley & Sons. This book was released on 2007-01-30 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-quality research articles on proteomic analyses of microbial pathogens, made available in a handy form. Containing proven, high-quality research articles selected from the popular PROTEOMICS journal, this is a current overview of the latest research into the proteomics analysis of microbial pathogens as well as several review articles.

Identification of Microorganisms by Mass Spectrometry

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Publisher : John Wiley & Sons
ISBN 13 : 0471748633
Total Pages : 377 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Identification of Microorganisms by Mass Spectrometry by : Charles L. Wilkins

Download or read book Identification of Microorganisms by Mass Spectrometry written by Charles L. Wilkins and published by John Wiley & Sons. This book was released on 2005-12-16 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms. A vital tool for microbiologists, health professionals, and analytical chemists, the text is designed to help scientists select the most effective techniques for use in biomedical, biochemical, pharmaceutical, and bioterror defense applications. Since microbiological applications of mass spectrometry require a basic understanding of both microbiology and analytical chemistry, the editors have incorporated material from both disciplines so that readers from either field will come to understand the necessary principles of the other. Featuring contributions from some of the most recognized experts in both fields, this volume provides specific examples of fundamental methods as well as approaches developed in the last decade, including: * Metastable atom bombardment pyrolysis mass spectrometry * Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) * MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria * High-resolution Fourier transform mass spectrometry (FTMS) * Electrospray ionization (ESI) mass spectrometry Identification of Microorganisms by Mass Spectrometry represents the most comprehensive and up-to-date work on the topic currently available. It is liberally illustrated with figures and tables and covers every aspect of spectrometric identification of microorganisms, including experimental procedures, various means of sample preparation, data analysis, and interpretation of complex mass spectral data.

Microbes

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Publisher : John Wiley & Sons
ISBN 13 : 1118311892
Total Pages : 731 pages
Book Rating : 4.1/5 (183 download)

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Book Synopsis Microbes by : Prakash S. Bisen

Download or read book Microbes written by Prakash S. Bisen and published by John Wiley & Sons. This book was released on 2012-06-22 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: An accessible introduction to the world of microbes—from basic microbe biology through industrial applications Microbes affect our lives in a variety of ways—playing an important role in our health, food, agriculture, and environment. While some microbes are beneficial, others are pathogenic or opportunistic. Microbes: Concepts and Applications describes basic microbe biology and identification and shows not only how they operate in the subfields of medicine, biotechnology, environmental science, bioengineering, agriculture, and food science, but how they can be harnessed as a resource. It provides readers with a solid grasp of etiologic agents, pathogenic processes, epidemiology, and the role of microbes as therapeutic agents. Placing a major emphasis on omics technology, the book covers recent developments in the arena of microbes and discusses their role in industry and agriculture, as well as in related fields such as immunology, cell biology, and molecular biology. It offers complete discussions of the major bacterial, viral, fungal, and parasitic pathogens; includes information on emerging infectious diseases, antibiotic resistance, and bioterrorism; and talks about the future challenges in microbiology. The most complete treatment of microbial biology available, Microbes features eye-opening chapters on: Human and Microbial World Gene Technology: Application and Techniques Molecular Diagnostic and Medical Microbiology Identification and Classification of Microbes Diversity of Microorganisms Microbes in Agriculture Microbes as a Tool for Industry and Research Complete with charts and figures, this book is an invaluable textbook for university teachers, students, researchers, and people everywhere who care about microorganisms.

Modern Analytical Techniques

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Publisher : Academic Press
ISBN 13 : 1483220915
Total Pages : 272 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Modern Analytical Techniques by : Gunter Zweig

Download or read book Modern Analytical Techniques written by Gunter Zweig and published by Academic Press. This book was released on 2013-10-22 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical Methods for Pesticides and Plant Growth Regulators, Volume XIV: Modern Analytical Techniques covers an updated treatment of the most frequently used techniques for pesticide analysis, i.e., thin-layer chromatography, gas chromatography (packed and capillary columns), high-performance liquid chromatography, and mass spectrometry. People involved in the analysis of pesticides will find the book useful.

Methodologies for Metabolomics

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Publisher : Cambridge University Press
ISBN 13 : 0521765900
Total Pages : 641 pages
Book Rating : 4.5/5 (217 download)

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Book Synopsis Methodologies for Metabolomics by : Norbert W. Lutz

Download or read book Methodologies for Metabolomics written by Norbert W. Lutz and published by Cambridge University Press. This book was released on 2013-01-21 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

Introduction to Geomicrobiology

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Publisher : John Wiley & Sons
ISBN 13 : 1444309021
Total Pages : 440 pages
Book Rating : 4.4/5 (443 download)

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Book Synopsis Introduction to Geomicrobiology by : Kurt O. Konhauser

Download or read book Introduction to Geomicrobiology written by Kurt O. Konhauser and published by John Wiley & Sons. This book was released on 2009-03-12 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to Geomicrobiology is a timely and comprehensive overview of how microbial life has affected Earth’s environment through time. It shows how the ubiquity of microorganisms, their high chemical reactivity, and their metabolic diversity make them a significant factor controlling the chemical composition of our planet. The following topics are covered: how microorganisms are classified, the physical constraints governing their growth, molecular approaches to studying microbial diversity, and life in extreme environments bioenergetics, microbial metabolic capabilities, and major biogeochemical pathways chemical reactivity of the cell surface, metal sorption, and the microbial role in contaminant mobility and bioremediation/biorecovery microbiological mineral formation and fossilization the function of microorganisms in mineral dissolution and oxidation, and the industrial and environmental ramifications of these processes elemental cycling in biofilms, formation of microbialites, and sediment diagenesis the events that led to the emergence of life, evolution of metabolic processes, and the diversification of the biosphere. Artwork from the book is available to instructors at www.blackwellpublishing.com/konhauser.

Principles and Applications of Clinical Mass Spectrometry

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Publisher : Elsevier
ISBN 13 : 0128160640
Total Pages : 218 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Principles and Applications of Clinical Mass Spectrometry by : Nader Rifai

Download or read book Principles and Applications of Clinical Mass Spectrometry written by Nader Rifai and published by Elsevier. This book was released on 2018-06-26 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is a concise resource for quick implementation of mass spectrometry methods in clinical laboratory work. Focusing on the practical use of these techniques, the first half of the book covers principles of chromatographic separations, principles and types of mass spectrometers, and sample preparation for analysis; the second half outlines the main applications of this technology within clinical laboratory settings, including determination of small molecules and peptides, as well as pathogen identification. A thorough yet succinct guide to using mass spectrometry technology in the clinical laboratory, Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is an essential resource for chemists, pharmaceutical and biotech researchers, certain government agencies, and standardization groups. Provides concrete examples of the main applications of mass spectrometry technology Describes current capabilities of the LC- and MS-based analytical methods Details methods for successful analytical work in the field

Microbiomes of Soils, Plants and Animals

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Publisher :
ISBN 13 : 9781108654418
Total Pages : pages
Book Rating : 4.6/5 (544 download)

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Book Synopsis Microbiomes of Soils, Plants and Animals by : Rachael E. Antwis

Download or read book Microbiomes of Soils, Plants and Animals written by Rachael E. Antwis and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A comparative, holistic synthesis of microbiome research, spanning soil, plant, animal and human hosts.

NMR-based Metabolomics

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Publisher : Royal Society of Chemistry
ISBN 13 : 184973643X
Total Pages : 384 pages
Book Rating : 4.8/5 (497 download)

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Book Synopsis NMR-based Metabolomics by : Hector C Keun

Download or read book NMR-based Metabolomics written by Hector C Keun and published by Royal Society of Chemistry. This book was released on 2018-01-17 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the state of the art in the application of NMR spectroscopy to metabolomics and will be a key title for researchers and practitioners.

Molecular Biology of The Cell

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Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of The Cell by : Bruce Alberts

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: