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The Use Of Hplc In Receptor Biochemistry
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Book Synopsis The Use of HPLC in Receptor Biochemistry by : Anthony R. Kerlavage
Download or read book The Use of HPLC in Receptor Biochemistry written by Anthony R. Kerlavage and published by Wiley-Liss. This book was released on 1989-11-10 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a concise background in HPLC methodology and includes illustrative examples of some of the key advances made in the receptor field through application of this chromatographic technique. A brief historical overview puts into perspective the specific state of the art HPLC laboratory applications that are contained in the book. Technical contributions discuss how best to select the HPLC mode for a particular biochemical problem, including the most effective use of a particularly sensitive technique, microbore HPLC for synthesis, purification, and characterization of synthetic peptides.
Book Synopsis Receptor Biochemistry by : E. C. Hulme
Download or read book Receptor Biochemistry written by E. C. Hulme and published by IRL Press. This book was released on 1990 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: The acquisition of three-dimensional structural information about receptors, particularly through the application of crystallographic and other physical techniques requires the purification and handling of significant amounts of receptor proteins. This book focuses particularly on strategies which have proven successful in the purification of a number of receptors of pharmacological importance. Included are examples of C-protein-coupled receptors, oligomeric receptors which incorporate an integral ion channel, and receptor tyrosine kinases. Specific aspects include the choice of detergents for solubilization, the synthesis and utilization of specific affinity gels, and peptide mapping, among other key topics. There is also an introduction to structure prediction from receptor sequences, and the high-level expression of cloned receptor genes. The book draws on the experience and insight of a number of scientists who have been at the forefront of progress in this difficult and experimentally demanding field.
Book Synopsis High-Performance Liquid Chromatography of Peptides and Proteins by : Colin T. Mant
Download or read book High-Performance Liquid Chromatography of Peptides and Proteins written by Colin T. Mant and published by CRC Press. This book was released on 2017-11-22 with total page 887 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.
Download or read book Fundamentals and Techniques written by and published by Elsevier. This book was released on 1991-11-26 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals and Techniques
Book Synopsis Handbook of Affinity Chromatography by : Toni Kline
Download or read book Handbook of Affinity Chromatography written by Toni Kline and published by CRC Press. This book was released on 1993-06-16 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Book Synopsis Steroid Hormone Receptors: Basic and Clinical Aspects by : V. K. Moudgil
Download or read book Steroid Hormone Receptors: Basic and Clinical Aspects written by V. K. Moudgil and published by Springer Science & Business Media. This book was released on 2013-03-13 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past few years have witnessed the emergence of steroid hormones as the wonder molecules which generate as much discussion in the scientific literature as they do in a typical living room. This transition has been a result of the tremendous public and scientific interest in the normal functioning of the hor mones as well their suggested involvement in several clinical conditions. In the recent past, notable scientific and technological advances have been made in the areas of contraception and regulation of fertility. Steroid receptors are the indis pensable mediators of hormonal responses and are complex protein molecules which appear to exist in association with other, yet undefined, proteins and/or factors. Receptors for vitamin D, retinoic acid and the thyroid hormones share structural similarities with steroid receptors, and the roster of this superfamily is still expanding. While our knowledge of the diversity and magnitude of steroid effects has advanced, the precise mode of steroid hormone action has alluded investigators. This volume brings together an international team of prominent investigators who discuss their most recent work on the basic and clinical aspects of steroid/nuclear receptors. The contributions represent updated versions of the invited presentations made at The Second Meadow Brook Conference on Steroid Receptors in Health and Disease. I am grateful to my colleagues on the Scientific Committee: Etienne Baulieu, Jack Gorski, Benita Katzenellenbogen, David Toft and James WittJiff, who provided the vision and guidance in formulating an out standing program.
Book Synopsis Published Scientific Papers of the National Institutes of Health by : National Institutes of Health (U.S.)
Download or read book Published Scientific Papers of the National Institutes of Health written by National Institutes of Health (U.S.) and published by . This book was released on 1990 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents the broad outline of NIH organizational structure, theprofessional staff, and their scientific and technical publications covering work done at NIH.
Book Synopsis Handbook of Affinity Chromatography by : David S. Hage
Download or read book Handbook of Affinity Chromatography written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph
Book Synopsis Neuroscience Methods by : Rosemary Martin
Download or read book Neuroscience Methods written by Rosemary Martin and published by CRC Press. This book was released on 2019-08-16 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unique in its coverage of such an extensive range of methods, Neuroscience Methods: A Guide for Advanced Students provides easy-to-understand descriptions of the many different techniques that are currently being used to study the brain at the molecular and cellular levels. This valuable reference text will help rescue undergraduate and postgraduate students from continuing bewilderment at the methods sections of current neuroscience publications. Topics covered include in vivo and in vitro preparations, electrophysiological, histochemical, hybridization and genetic techniques, measurement of cellular ion concentrations, methods of drug application, production of antibodies, expression systems, and neural grafting.
Book Synopsis Analytical and Preparative Separation Methods of Biomacromolecules by : Hassan Y. Aboul-Enein
Download or read book Analytical and Preparative Separation Methods of Biomacromolecules written by Hassan Y. Aboul-Enein and published by CRC Press. This book was released on 2020-10-15 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.
Book Synopsis Bioaffinity Chromatography by : J. Turková
Download or read book Bioaffinity Chromatography written by J. Turková and published by Elsevier. This book was released on 1993-09-14 with total page 819 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography. Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.
Download or read book Receptor Molecular Biology written by and published by Elsevier. This book was released on 1995-03-22 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Cloning Expression systems Signal transduction Structure-function techniques Antireceptor antibodies Regulation 3-D receptor modeling and computational probing
Book Synopsis The Histamine Receptor by : J. C. Schwartz
Download or read book The Histamine Receptor written by J. C. Schwartz and published by . This book was released on 1992-01-24 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthesizes the latest information and research involving the pharmacochemistry, biochemical properties and neurobiological function of histamine receptors. Contains over 50 illustrations.
Book Synopsis Biochemical Studies of CNS Receptors by : Leslie Iversen
Download or read book Biochemical Studies of CNS Receptors written by Leslie Iversen and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is now eight years since the first Handbook volumes on Basic Neuro pharmacology were published, and there have been many important advances. As in many other areas in science, progress in this field has depended to a considerable extent on the availability of new experimental methods, and Volume 15 reviews some major recent developments, including new autoradiographic techniques that allow direct visualization of drug and transmitter receptors in the nervous system, and the pin pointing of the precise locations of the changes in brain metabolism elicited by various drug treatments. Volume 16 and 17 cover two of the most active areas for basic research in psychopharmacology at the moment: the characterization of drug and transmitter receptors in brain by radioligand binding techniques, and studies of the role of small peptides in brain function. The latter area, in particular, illustrates how rapidly progress continues to be made in basic research on the mechanisms of chemical communication within the nervous system. Eight years ago when the Handbook first appeared none of the opioid peptides (enkephalins and endorphins) had yet been identified. Since then a whole new area of basic biological research has focused on these substances, and in addition we know of more than thirty other neuropeptides with putative eNS trans mitter functions.
Book Synopsis Advanced Chromatographic and Electromigration Methods in BioSciences by : I. Mikšík
Download or read book Advanced Chromatographic and Electromigration Methods in BioSciences written by I. Mikšík and published by Elsevier. This book was released on 1998-09-28 with total page 1149 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted. Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.
Book Synopsis The Utilization of Microcolumn High-performance Liquid Chromatography in the Study of Proteins by : Cheryl Lynn Flurer
Download or read book The Utilization of Microcolumn High-performance Liquid Chromatography in the Study of Proteins written by Cheryl Lynn Flurer and published by . This book was released on 1992 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Practical High-Performance Liquid Chromatography by : Veronika Meyer
Download or read book Practical High-Performance Liquid Chromatography written by Veronika Meyer and published by . This book was released on 1994-04-12 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Updated and improved this edition discusses both practical and theoretical aspects along with the possibilities and problems associated with HPLC. Reflects numerous new details and recent advancements in HPLC techniques. Offers the latest information on current chromatographic practice and covers equipment preparation, column packing and regeneration, commercially available stationary phases and much more.
