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The Role Of The Rna Binding Protein Bruno During Oogenesis In Drosophila Melanogaster
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Book Synopsis The Role of the RNA-binding Protein Bruno During Oogenesis in Drosophila Melanogaster by : Paolo Filardo
Download or read book The Role of the RNA-binding Protein Bruno During Oogenesis in Drosophila Melanogaster written by Paolo Filardo and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Role of the RNA-binding Protein Bruno During Oogenesis in Drosophila Melanogster by : Paolo Filardo
Download or read book The Role of the RNA-binding Protein Bruno During Oogenesis in Drosophila Melanogster written by Paolo Filardo and published by . This book was released on 2002 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Bruno Contributes to Osk MRNA Localization During Drosophila Oogenesis by : Youme Gim
Download or read book Bruno Contributes to Osk MRNA Localization During Drosophila Oogenesis written by Youme Gim and published by . This book was released on 2011 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oskar is a body pattern and germ cell determining protein in Drosophila melanogaster. This protein must be properly expressed to ensure correct formation of the posterior pole of the animal. Bruno is a RNA binding protein known to regulate osk mRNA translation during Drosophila oogenesis. In vitro work has implicated Bru in oligomerizing osk mRNA into silencing particles and thereby preventing osk mRNA from accessing translational machinery and inhibiting translation. In attempts to further investigate Bru mediated translational regulation, reporter transcripts with Bruno binding regions from the osk mRNA were analyzed for translation and localization of the transcript. Localization of these reporter transcripts has shown the first in vivo evidence for the Bru mediated silencing particle assembly model. In this thesis, I report on the distribution of reporter transcripts in the Drosophila egg chamber.
Book Synopsis Bruno Regulates MRNA Translation by Binding to Multiple Sequence Motifs by : Bradley Steven Reveal
Download or read book Bruno Regulates MRNA Translation by Binding to Multiple Sequence Motifs written by Bradley Steven Reveal and published by . This book was released on 2010 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oskar (Osk) is a posterior body patterning determinant in Drosophila melanogaster oocytes. oskar (osk) mRNA is translationally repressed until it reaches the posterior of the oocyte where Osk protein accumulates. Translational repression of osk prior to posterior localization is mediated by the RNA binding protein, Bruno (Bru). To better define Bru binding sites, I performed in vitro selections using full length Bru and the fragments containing either the first two RRMs (RRM1+2) or the third RRM (RRM3+). The aptamers from the final round from each of the selections produced a multitude of overrepresented primary sequence motifs. Examples of each of these motifs were found in the 3'UTRs of the mRNAs that Bru is known to regulate during oogenesis. GFP reporter transgenes under the control of the UAS-Gal4 expression system were constructed with each class of the binding sites within the reporter transgenes' 3'UTRs to test the motifs' ability to repress the reporters in vivo. In a wildtype background, the GFP reporters containing the binding sites were translationally repressed. In the aret mutant background, the GFP levels of the repressed GFP reporters increased with reduced Bru activity, suggesting the transgenes' repression is mediated by Bru. Three of the motifs isolated in the in vitro selections reside in the AB and C regions of the osk 3'UTR, and the three classes of sites were mutated in the AB and C regions. The mutated AB and C regions were used to assay for a reduction of Bru binding affinity for the mutant RNAs. Additionally, the mutations were incorporated into an osk genomic transgene that was introduced into an osk RNA null as well as an Osk protein null background. The mutations reduced Bru binding to the AB and C regions. The transgenes containing the mutated Bru binding sites could not fully rescue the osk RNA null phenotype but can fully rescue the Osk protein null phenotype, suggesting an osk transcript can regulate other osk mRNAs in trans.
Book Synopsis Genetic and Molecular Characterization of the Putative RNA-binding Protein Orb During Drosophila Oogenesis by : Lori Bennett Christerson
Download or read book Genetic and Molecular Characterization of the Putative RNA-binding Protein Orb During Drosophila Oogenesis written by Lori Bennett Christerson and published by . This book was released on 1995 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Cell Lineage and Embryo Patterning by :
Download or read book Cell Lineage and Embryo Patterning written by and published by Elsevier. This book was released on 2000-12-13 with total page 623 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Review of Cytology presents current advances and comprehensive reviews in cell biology, both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. This volume brings together current information on the localization and roles of RNAs in cell-lineage determination and subsequent patterning in embryonic development. Guest co-editor Lawrence Etkin is one of the leading researchers in molecular genetics of Xenopus. Key Features * A number of important concepts are discussed, including: * How polarity is established during oogenesis * How germ cell determinants become organized in the establishment of the germ cell lineage * Different strategies used by organisms to establish the germ cell lineage * Similarities and differences between the mechanisms used in embryonic patterning * The mechanisms and machinery by which molecules such as RNA become asymmetrically segregated * The use of similar signaling pathways in patterning of the dorsal-ventral and right-left asymmetries, embryonic germ layers, limb, and nervous system * The link between fundamental biological processes such as RNA translation and localization in the regulation of axis specification
Book Synopsis Drosophila melanogaster by : Farzana Khan Perveen
Download or read book Drosophila melanogaster written by Farzana Khan Perveen and published by BoD – Books on Demand. This book was released on 2018-02-28 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains 12 chapters divided into two sections. Section 1 is "Drosophila - Model for Genetics." It covers introduction, chromosomal polymorphism, polytene chromosomes, chromosomal inversion, chromosomal evolution, cell cycle regulators in meiosis and nongenetic transgenerational inheritance in Drosophila. It also includes ecological genetics, wild-type strains, morphometric analysis, cytostatics, frequencies of early and late embryonic lethals (EEL and LEL) and mosaic imaginal discs of Drosophila for genetic analysis in biomedical research. Section 2 is "Drosophila - Model for Therapeutics." It explains Drosophila as model for human diseases, neurodegeneration, heart-kidney metabolic disorders, cancer, pathophysiology of Parkinson's disease, dopamine, neuroprotective therapeutics, mitochondrial dysfunction and translational research. It also covers Drosophila role in ubiquitin-carboxyl-terminal hydrolase-L1 (UCH-L1) protein, eye development, anti-dUCH antibody, neuropathy target esterase (NTE), organophosphorous compound-induced delayed neuropathy (OPIDN) and hereditary spastic paraplegia (HSP). It also includes substrate specificities, kinetic parameters of recombinant glutathione S-transferases E6 and E7 (DmGSTE6 and DmGSTE7), detoxification and insecticidal resistance and antiviral immunity in Drosophila.
Book Synopsis Localized RNAs by : Howard D. Lipshitz
Download or read book Localized RNAs written by Howard D. Lipshitz and published by R G Landes Company. This book was released on 1995 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Oocytes written by Malgorzata Kloc and published by Springer. This book was released on 2017-08-02 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book combines the most recent knowledge on the maternal, i.e. oocyte/egg-specific, molecules and processes. The volume covers the most recent advances in a plethora of subjects such as: maternal transfer of immunity, localized RNAs functions and mechanisms of RNA localization, transcriptional repression of maternal messages, maternal inheritance and maternal role of CRISPR/Cas9-based genome editing, chromatin remodeling and epigenetic modifications, maternal function of nucleosomes, maternal mitochondria and energy supply, role of bacterial symbionts and their maternal transmission, acquisition of oocyte polarity and evolution of maternal effect genes, germ plasm and oosome origin and functions, mechanisms of oocyte activation and soma germ cells communication. Currently, no other book on the market combines such a comprehensive list of subjects in one volume. Moreover, the information provided is a cross-section through oocytes from various invertebrate and vertebrate species, which is another unique feature of this book. The readers, therefore, get a completely new and invaluable perspective on all covered subjects.
Book Synopsis Biophysical approaches to translational control of gene expression by : Jonathan D. Dinman
Download or read book Biophysical approaches to translational control of gene expression written by Jonathan D. Dinman and published by Springer Science & Business Media. This book was released on 2012-09-20 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a premier resource on understanding the ribosome's essential nature and how it interacts with other proteins and nucleic acids to control protein synthesis. As one of the central foundations in our understanding of the biology at the molecular level, this topic appeals to a wide audience, from bench researcher to clinician. With the advent of atomic scale structures, methods to visualize and separate individual molecules, and the computational power to model the complex interactions of over a million atoms at once, our understanding of how gene expression is controlled at the level of protein translation is now deeply ensconced in the biophysical realm.
Author :Rabindra N. Chatterjee Publisher :Springer Science & Business Media ISBN 13 :3662118297 Total Pages :262 pages Book Rating :4.6/5 (621 download)
Book Synopsis Genome Analysis in Eukaryotes by : Rabindra N. Chatterjee
Download or read book Genome Analysis in Eukaryotes written by Rabindra N. Chatterjee and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the last decades a breakthrough in the understanding of the mechanisms controlling development has been achieved. This has been possible, in great part, by the use of various experimental approaches. This book focuses on topics concerning some of the processes involved in development, the main emphasis being on the genetic and molecular mechanisms in the evolutionary context. Drosophila is used as an experimental model for the genetic approach to the understanding of behaviour.
Book Synopsis Drosophila Oogenesis by : Diana P. Bratu
Download or read book Drosophila Oogenesis written by Diana P. Bratu and published by Humana. This book was released on 2015-09-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis A Comparative Methods Approach to the Study of Oocytes and Embryos by : Joel D. Richter
Download or read book A Comparative Methods Approach to the Study of Oocytes and Embryos written by Joel D. Richter and published by Oxford University Press, USA. This book was released on 1999-01 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past twenty years, biologists have made remarkable progress in understanding each step as a single fertilized egg develops into a mature organism, and the research draws on a tremendous array of experimental techniques. A Comparative Methods Approach to the Study of Oocytes and Embryos takes a unique approach to the field, providing a hands-on guide to three of the most important experimental subjects--frogs, fruitflies, and mice. By collecting reliable techniques in a single volume, the book is both an invaluable resource for new researchers and an ideal platform for comparing these seemingly disparate biological systems. The volume contains work from many leaders in the field and spans research in genetics, molecular and cellular biology, and morphology. It includes methods for oocyte and embryo culture, microinjection, in situ hybridization, immunohistochemistry, time-lapse microscopy, intracellular ion measurements, and embryonic dissections.
Book Synopsis Translational Control in Health and Disease by :
Download or read book Translational Control in Health and Disease written by and published by Academic Press. This book was released on 2009-10-26 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress in Molecular Biology and Translational Science provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology and translational science. It contains contributions from leaders in their fields and abundant references. This volume focuses on translational control in health and disease. Contributions from leading scholars and industry experts Reference guide for researchers involved in molecular biology and related fields
Book Synopsis International Review of Cytology by : Kwang W. Jeon
Download or read book International Review of Cytology written by Kwang W. Jeon and published by Elsevier. This book was released on 2006-02-20 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Review of Cytology presents current advances and comprehensive reviews in cell biology – both plant and animal. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Articles in this volume address new insights into fatty acid modulation of pancreatic beta cell function; drosophila RNA binding proteins; plasticity of pelvic autonomic ganglia and urogenital innervation; vomeronasal vs. olifactory epithelium: cellular basis for human vomeronasal perception; and tight junctions.
Book Synopsis Signaling-Mediated Control of Cell Division by : Swathi Arur
Download or read book Signaling-Mediated Control of Cell Division written by Swathi Arur and published by Springer. This book was released on 2017-02-28 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers the current knowledge base on the role of signaling and environmental pathways that control the normal development of germline stem cells, meiotic progression of oocytes, events of oocyte maturation and fertilization, and the birth of an embryo. Germ cells are uniquely poised to sustain life across generations through the fusion of oocyte and sperm. Because of the central importance of germ cells to life, much work has been dedicated to obtaining a clear understanding of the molecular and signaling events that control their formation and maintenance. Germ cells are set aside from somatic cells in the embryo and go through specialized meiotic cell cycles as the animal matures. These cell cycles are interspersed with long periods of arrest. In human females, meiosis I is initiated in the fetus. At birth, oocytes are arrested in meiosis I; after puberty, every month an oocyte initiates meiosis II – ovulation. Upon sperm availability these cells are fertilized, generate an embryo, and the cycle-of-life continues. During meiotic I progression and arrest, the fitness of oocytes and their progeny are likely influenced by environmental cues and signaling pathways. A lot of recent work has focused on understanding the mechanisms that regulate oocyte fitness and quality in humans and vertebrates. Much of our understanding on the events of meiosis I and germline stem cell populations comes from work in invertebrates, wherein the germline stem cells produce oocytes continuously through adult development. In both inverbrates and vertebrates nutritional and signaling pathways control the regulation of stem cells in such a manner so as to couple production of gametes with the nutritional availability. Additionally, mature oocytes arrest both in meiosis I and meiosis II, and signaling and nutritional pathways have been shown to regulate their formation, and maintenance, such that despite long periods of arrest, the oocyte quality is assured and errors in chromosome segregation and varied cytoplasmic events are minimal.
Book Synopsis Germ Cell Development and Reproductive Aging by : Miguel Angel Brieño-Enriquez
Download or read book Germ Cell Development and Reproductive Aging written by Miguel Angel Brieño-Enriquez and published by Frontiers Media SA. This book was released on 2022-07-14 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: