The Multiplexing Of Assays For The Measurement Of Early Stages Of Apoptosis By Polychromatic Flow Cytometry

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The Multiplexing of Assays for the Measurement of Early Stages of Apoptosis by Polychromatic Flow Cytometry

Author : G. Warnes
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (115 download)

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Book Synopsis The Multiplexing of Assays for the Measurement of Early Stages of Apoptosis by Polychromatic Flow Cytometry by : G. Warnes

Download or read book The Multiplexing of Assays for the Measurement of Early Stages of Apoptosis by Polychromatic Flow Cytometry written by G. Warnes and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The detection of apoptosis has been a stalwart application for flow cytometric analysis for decades and this review of flow cytometric methods to detect early stages of apoptosis includes the use of the pivotal assay to detect early and late apoptosis, the Annexin V assay which when multiplexed with biologically functional fluorescent dyes to measure mitochondrial function and Reactive Oxygen Species (ROS) generation allows further identification of functionally different subsets within apoptotic populations. Here we show how this polychromatic approach can be used to demonstrate which subset of cells show changes in mitochondrial function and when ROS is generated in a time dependent manner. This polychromatic approach to flow cytometry leads to the identification of over ten sub-populations of cells during classic apoptosis or programmed cell death (PCD).

Flow Cytometry

Author : Ingrid Schmid
Publisher : BoD – Books on Demand
ISBN 13 : 9535125508
Total Pages : 166 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Flow Cytometry by : Ingrid Schmid

Download or read book Flow Cytometry written by Ingrid Schmid and published by BoD – Books on Demand. This book was released on 2016-08-24 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry - Select Topics is a collection of chapters that illustrate the constantly evolving application of flow cytometry to diverse areas of research or clinical investigations. It includes chapters on the utilization of flow cytometry in the fields of human reproduction and fertility, platelet function, apoptosis, inflammation research, leukemia immunophenotyping, and transplantation.

Flow Cytometry and Cell Sorting

Author : Andreas Radbruch
Publisher : Springer Science & Business Media
ISBN 13 : 3662041294
Total Pages : 365 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Flow Cytometry and Cell Sorting by : Andreas Radbruch

Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Techniques in Cell Cycle Analysis

Author : Joe W. Gray
Publisher : Springer Science & Business Media
ISBN 13 : 1603274065
Total Pages : 419 pages
Book Rating : 4.6/5 (32 download)

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Book Synopsis Techniques in Cell Cycle Analysis by : Joe W. Gray

Download or read book Techniques in Cell Cycle Analysis written by Joe W. Gray and published by Springer Science & Business Media. This book was released on 2008-02-24 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantification of the proliferative characteristics of normal and malignant cells has been of interest to oncolo gists and cancer biologists for almost three decades. This interest stems from (a) the fact that cancer is a disease of uncontrolled proliferation, (b) the finding that many of the commonly used anticancer agents are preferentially toxic to cells that are actively proliferating, and (c) the observa tion that significant differences in proliferation characteristics exist between normal and malignant cells. Initially, cell cycle analysis was pursued enthusiastically in the hope of gener ating information useful for the development of rational cancer therapy strategies; for example, by allowing identi fication of rapidly proliferating tumors against which cell cycle-specific agents could be used with maximum effec tiveness and by allowing rational scheduling of cell cyc- specific therapeutic agents to maximize the therapeutic ratio. Unfortunately, several difficulties have prevented realiza tion of the early promise of cell cycle analysis: Proliferative patterns of the normal and malignant tissues have been found to be substantially more complex than originally an ticipated, and synchronization of human tumors has proved remarkably difficult. Human tumors of the same type have proved highly variable, and the cytokinetic tools available for cell cycle analysis have been labor intensive, as well as somewhat subjective and in many cases inapplicable to humans. However, the potential for substantially improved cancer therapy remains if more accurate cytokinetic infor mation about human malignancies and normal tissues can be obtained in a timely fashion.

Flow Cytometry

Author : Marion G. Macey
Publisher : Springer Science & Business Media
ISBN 13 : 1597454516
Total Pages : 289 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Flow Cytometry by : Marion G. Macey

Download or read book Flow Cytometry written by Marion G. Macey and published by Springer Science & Business Media. This book was released on 2007-11-03 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Immunophenotyping

Author : J Philip McCoy Jr
Publisher : Humana
ISBN 13 : 9781493996490
Total Pages : 375 pages
Book Rating : 4.9/5 (964 download)

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Book Synopsis Immunophenotyping by : J Philip McCoy Jr

Download or read book Immunophenotyping written by J Philip McCoy Jr and published by Humana. This book was released on 2019-09-29 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.

Practical Flow Cytometry

Author : Howard M. Shapiro
Publisher : John Wiley & Sons
ISBN 13 : 0471434035
Total Pages : 736 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Practical Flow Cytometry by : Howard M. Shapiro

Download or read book Practical Flow Cytometry written by Howard M. Shapiro and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Single Cell Protein Analysis

Author : Anup K. Singh
Publisher : Humana
ISBN 13 : 9781493929863
Total Pages : 0 pages
Book Rating : 4.9/5 (298 download)

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Book Synopsis Single Cell Protein Analysis by : Anup K. Singh

Download or read book Single Cell Protein Analysis written by Anup K. Singh and published by Humana. This book was released on 2015-11-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.

Introduction to Biophotonics

Author : Paras N. Prasad
Publisher : John Wiley & Sons
ISBN 13 : 0471465399
Total Pages : 622 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Introduction to Biophotonics by : Paras N. Prasad

Download or read book Introduction to Biophotonics written by Paras N. Prasad and published by John Wiley & Sons. This book was released on 2004-01-16 with total page 622 pages. Available in PDF, EPUB and Kindle. Book excerpt: Paras Prasad’s text provides a basic knowledge of a broad range of topics so that individuals in all disciplines can rapidly acquire the minimal necessary background for research and development in biophotonics. Introduction to Biophotonics serves as both a textbook for education and training as well as a reference book that aids research and development of those areas integrating light, photonics, and biological systems. Each chapter contains a topic introduction, a review of key data, and description of future directions for technical innovation. Introduction to Biophotonics covers the basic principles of Optics Optical spectroscopy Microscopy Each section also includes illustrated examples and review questions to test and advance the reader’s knowledge. Sections on biosensors and chemosensors, important tools for combating biological and chemical terrorism, will be of particular interest to professionals in toxicology and other environmental disciplines. Introduction to Biophotonics proves a valuable reference for graduate students and researchers in engineering, chemistry, and the life sciences.

High-Dimensional Single Cell Analysis

Author : Harris G. Fienberg
Publisher : Springer
ISBN 13 : 364254827X
Total Pages : 224 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis High-Dimensional Single Cell Analysis by : Harris G. Fienberg

Download or read book High-Dimensional Single Cell Analysis written by Harris G. Fienberg and published by Springer. This book was released on 2014-04-22 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is an invaluable reference book for both basic and clinical researchers.

Apoptosis and Beyond

Author : James A. Radosevich
Publisher : John Wiley & Sons
ISBN 13 : 1119432359
Total Pages : 768 pages
Book Rating : 4.1/5 (194 download)

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Book Synopsis Apoptosis and Beyond by : James A. Radosevich

Download or read book Apoptosis and Beyond written by James A. Radosevich and published by John Wiley & Sons. This book was released on 2018-09-18 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: These volumes teach readers to think beyond apoptosis and describes all of the known processes that cells can undergo which result in cell death This two-volume source on how cells dies is the first, comprehensive collection to cover all of the known processes that cells undergo when they die. It is also the only one of its kind to compare these processes. It seeks to enlighten those in the field about these many processes and to stimulate their thinking at looking at these pathways when their research system does not show signs of activation of the classic apoptotic pathway. In addition, it links activities like the molecular biology of one process (eg. Necrosis) to another process (eg. apoptosis) and contrasts those that are close to each. Volume 1 of Apoptosis and Beyond: The Many Ways Cells Die begins with a general view of the cytoplasmic and nuclear features of apoptosis. It then goes on to offer chapters on targeting the cell death mechanism; microbial programmed cell death; autophagy; cell injury, adaptation, and necrosis; necroptosis; ferroptosis; anoikis; pyronecrosis; and more. Volume 2 covers such subjects as phenoptosis; pyroptosis; hematopoiesis and eryptosis; cyclophilin d-dependent necrosis; and the role of phospholipase in cell death. Covers all known processes that dying cells undergo Provides extensive coverage of a topic not fully covered before Offers chapters written by top researchers in the field Provides activities that link and contrast processes to each other Apoptosis and Beyond: The Many Ways Cells Die will appeal to students and researchers/clinicians in cell biology, molecular biology, oncology, and tumor biology.

Cancer and Noncoding RNAs

Author : Jayprokas Chakrabarti
Publisher : Academic Press
ISBN 13 : 0128134372
Total Pages : 578 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Cancer and Noncoding RNAs by : Jayprokas Chakrabarti

Download or read book Cancer and Noncoding RNAs written by Jayprokas Chakrabarti and published by Academic Press. This book was released on 2017-10-13 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer and Noncoding RNAs offers an in-depth exploration of noncoding RNAs and their role in epigenetic regulation of complex human disease, most notably cancer. In addition to examining microRNAs, this volume provides a unique evaluation of more recently profiled noncoding RNAs now implicated in carcinogenesis, including lncRNAs, piRNAs, circRNAs, and tRNAs, identifying differences in function between these noncoding RNAs and how they interact with the rest of the epigenome. A broad range of chapters from experts in the field detail epigenetic regulation of various cancer types, along with recent next generation sequencing technologies, genome-wide association studies (GWAS) and bioinformatics approaches. This book will help researchers in genomic medicine and cancer biology better understand the role of noncoding RNAs in epigenetics, aiding in the development of useful biomarkers for diagnosis, prognosis and new RNA-based disease therapies. Provides a comprehensive analysis of noncoding RNAs implicated in epigenetic regulation of gene expression and chromatin dynamics Educates researchers and graduate students by highlighting, in addition to miRNAs, a range of noncoding RNAs newly associated with carcinogenesis Applies current knowledge of noncoding RNAs and epigenomics towards developing cancer and RNA-based disease therapies Features contributions by leading experts in the field

Surface Plasmon Resonance Sensors

Author : Leiva Casemiro Oliveira
Publisher : Springer
ISBN 13 : 3319149261
Total Pages : 188 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Surface Plasmon Resonance Sensors by : Leiva Casemiro Oliveira

Download or read book Surface Plasmon Resonance Sensors written by Leiva Casemiro Oliveira and published by Springer. This book was released on 2015-02-23 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the important physical phenomenon of Surface Plasmon Resonance or Surface Plasmon Polaritons in thin metal films, a phenomenon which is exploited in the design of a large variety of physico-chemical optical sensors. In this treatment, crucial materials aspects for design and optimization of SPR sensors are investigated and outlined in detail. The text covers the selection of nanometer thin metal films, ranging from free-electron to the platinum type conductors, along with their combination with a large variety of dielectric substrate materials, and associated individual layer and opto-geometric arrangements. Furthermore, as-yet hardly explored SPR features of selected metal–metal and metal–dielectric super lattices are included in this report. An in-depth multilayer Fresnel evaluation provides the mathematical tool for this optical analysis, which otherwise relies solely on experimentally determined electro-optical materials parameters.

Flow Cytometry and Sorting

Author : Myron R. Melamed
Publisher : Wiley-Liss
ISBN 13 : 9780471562351
Total Pages : 836 pages
Book Rating : 4.5/5 (623 download)

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Book Synopsis Flow Cytometry and Sorting by : Myron R. Melamed

Download or read book Flow Cytometry and Sorting written by Myron R. Melamed and published by Wiley-Liss. This book was released on 1990-04-30 with total page 836 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revised and updated, this Second Edition of a classic text describes and evaluates--in greater detail--the most recent practical applications of flow cytometry technique to basic cellular biological investigations and clinical research on human neoplasms. Ideal for the experienced researcher as well as the novice, this informative book offers state-of-the-art reviews of all aspects of flow cytometry. New articles highlight investigations of higher plants, the flow cytometry of microorganisms, and measurements of intracellular ionized calcium and membrane potential--illustrating techniques of specimen preparation, measurement and analysis for each. New chapters examine applications of flow cytometry to medical genetics, genetic toxicology, and ultrasensitive analysis of molecules in solution. The Second Edition goes beyond the traditional analysis of DNA histograms with BrdU incorporation and DNA denaturability to identify and analyze the cell cycle more precisely. New or rewritten chapters discuss the importance of flow cytometry for measurements of nucleic acids, chromatin, and DNA and cover the cytometry of sperm and the cytopathic effects of viruses.

Dorland's Dictionary of Medical Acronyms and Abbreviations E-Book

Author : Dorland
Publisher : Elsevier Health Sciences
ISBN 13 : 0323442544
Total Pages : 488 pages
Book Rating : 4.3/5 (234 download)

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Book Synopsis Dorland's Dictionary of Medical Acronyms and Abbreviations E-Book by : Dorland

Download or read book Dorland's Dictionary of Medical Acronyms and Abbreviations E-Book written by Dorland and published by Elsevier Health Sciences. This book was released on 2015-07-24 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medical acronyms and abbreviations offer convenience, but those countless shortcuts can often be confusing. Now a part of the popular Dorland’s suite of products, this reference features thousands of terms from across various medical specialties. Its alphabetical arrangement makes for quick reference, and expanded coverage of symbols ensures they are easier to find. Effective communication plays an important role in all medical settings, so turn to this trusted volume for nearly any medical abbreviation you might encounter. Symbols section makes it easier to locate unusual or seldom-used symbols. Convenient alphabetical format allows you to find the entry you need more intuitively. More than 90,000 entries and definitions. Many new and updated entries including terminology in expanding specialties, such as Nursing; Physical, Occupational, and Speech Therapies; Transcription and Coding; Computer and Technical Fields. New section on abbreviations to avoid, including Joint Commission abbreviations that are not to be used. Incorporates updates suggested by the Institute for Safe Medication Practices (ISMP).

Biophotonics

Author : Gerd Keiser
Publisher : Springer
ISBN 13 : 9811009457
Total Pages : 362 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Biophotonics by : Gerd Keiser

Download or read book Biophotonics written by Gerd Keiser and published by Springer. This book was released on 2016-07-20 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces senior-level and postgraduate students to the principles and applications of biophotonics. It also serves as a valuable reference resource or as a short-course textbook for practicing physicians, clinicians, biomedical researchers, healthcare professionals, and biomedical engineers and technicians dealing with the design, development, and application of photonics components and instrumentation to biophotonics issues. The topics include the fundamentals of optics and photonics, the optical properties of biological tissues, light-tissue interactions, microscopy for visualizing tissue components, spectroscopy for optically analyzing the properties of tissue, and optical biomedical imaging. It also describes tools and techniques such as laser and LED optical sources, photodetectors, optical fibers, bioluminescent probes for labeling cells, optical-based biosensors, surface plasmon resonance, and lab-on-a-chip technologies. Among the applications are optical coherence tomography (OCT), optical imaging modalities, photodynamic therapy (PDT), photobiostimulation or low-level light therapy (LLLT), diverse microscopic and spectroscopic techniques, tissue characterization, laser tissue ablation, optical trapping, and optogenetics. Worked examples further explain the material and how it can be applied to practical designs, and the homework problems help test readers’ understanding of the text.

Mammalian Cell Viability

Author : Martin J. Stoddart
Publisher : Humana Press
ISBN 13 : 9781617791079
Total Pages : 0 pages
Book Rating : 4.7/5 (91 download)

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Book Synopsis Mammalian Cell Viability by : Martin J. Stoddart

Download or read book Mammalian Cell Viability written by Martin J. Stoddart and published by Humana Press. This book was released on 2011-04-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.