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Author : Michael Trent Schwemberger
Publisher :
ISBN 13 :
Total Pages : 196 pages
Book Rating : 4.:/5 (42 download)
Download or read book The Modulation of Enzyme Activity in Enzyme Based Sensors written by Michael Trent Schwemberger and published by . This book was released on 1990 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Alexander P. Demchenko
Publisher : Springer
ISBN 13 : 3319207806
Total Pages : 818 pages
Book Rating : 4.3/5 (192 download)
Download or read book Introduction to Fluorescence Sensing written by Alexander P. Demchenko and published by Springer. This book was released on 2015-10-06 with total page 818 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence is the most popular technique in chemical and biological sensing and this book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Its ultimate sensitivity, high temporal and spatial resolution and versatility enables high resolution imaging within living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response, up to the detection of single molecules. Its application areas range from the control of industrial processes to environmental monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.
Author : Antonio Baici
Publisher : Springer
ISBN 13 : 3709114020
Total Pages : 503 pages
Book Rating : 4.7/5 (91 download)
Download or read book Kinetics of Enzyme-Modifier Interactions written by Antonio Baici and published by Springer. This book was released on 2015-06-24 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.
Author : V.A. Najjar
Publisher : Springer Science & Business Media
ISBN 13 : 1461338794
Total Pages : 314 pages
Book Rating : 4.4/5 (613 download)
Download or read book Enzyme Induction and Modulation written by V.A. Najjar and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher : Academic Press
ISBN 13 : 0128092912
Total Pages : 532 pages
Book Rating : 4.1/5 (28 download)
Download or read book Enzymes as Sensors written by and published by Academic Press. This book was released on 2017-03-21 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes as Sensors, Volume 589, the latest release in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics that presents a micro-level delivery of specific content related to the study of enzymes in sensors. New to this edition are highly specialized chapters on integrated strategies for gaining a systems level view of dynamic signaling networks, sensitive protein detection and quantification in paper-based microfluidics for point-of-care, and microneedle enzyme sensor arrays for continuous in vivo monitoring. This state-of-the-art series is ideal for those interested in the latest information on enzymology, with this edition focusing on sensors and their role in enzymes. Contain contributions from leading authorities Informs and updates on all the latest developments in the field
Author :
Publisher :
ISBN 13 :
Total Pages : 6 pages
Book Rating : 4.:/5 (684 download)
Download or read book Immobilization, Stabilization and Patterning Techniques for Enzyme Based Sensor Systems written by and published by . This book was released on 1997 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sandia National Laboratories has recently opened the Chemical and Radiation Detection Laboratory (CRDL) in Livermore CA to address the detection needs of a variety of government agencies (e.g., Department of Energy, Environmental Protection Agency, Department of Agriculture) as well as provide a fertile environment for the cooperative development of new industrial technologies. This laboratory consolidates a variety of existing chemical and radiation detection efforts and enables Sandia to expand into the novel area of biochemically based sensors. One aspect of this biosensor effort is further development and optimization of enzyme modified field effect transistors (EnFETs). Recent work has focused upon covalent attachment of enzymes to silicon dioxide and silicon nitride surfaces for EnFET fabrication. They are also investigating methods to pattern immobilized proteins; a critical component for development of array-based sensor systems. Novel enzyme stabilization procedures are key to patterning immobilized enzyme layers while maintaining enzyme activity. Results related to maximized enzyme loading, optimized enzyme activity and fluorescent imaging of patterned surfaces will be presented.
Author : Paolo Bollella
Publisher : MDPI
ISBN 13 : 3039438875
Total Pages : 504 pages
Book Rating : 4.0/5 (394 download)
Download or read book Biosensors – Recent Advances and Future Challenges written by Paolo Bollella and published by MDPI. This book was released on 2021-01-27 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book is devoted to all aspects of biosensing in a very broad definition, including, but not limited to, biomolecular composition used in biosensors (e.g., biocatalytic enzymes, DNAzymes, abiotic nanospecies with biocatalytic features, bioreceptors, DNA/RNA, aptasensors, etc.), physical signal transduction mechanisms (e.g., electrochemical, optical, magnetic, etc.), engineering of different biosensing platforms, operation of biosensors in vitro and in vivo (implantable or wearable devices), self-powered biosensors, etc. The biosensors can be represented with analogue devices measuring concentrations of analytes and binary devices operating in the YES/NO format, possibly with logical processing of input signals. Furthermore, the book is aimed at attracting young scientists and introducing them to the field, while providing newcomers with an enormous collection of literature references.
Author : Alexandru Mihai Grumezescu
Publisher : Academic Press
ISBN 13 : 0128043709
Total Pages : 792 pages
Book Rating : 4.1/5 (28 download)
Download or read book New Pesticides and Soil Sensors written by Alexandru Mihai Grumezescu and published by Academic Press. This book was released on 2017-07-07 with total page 792 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Pesticides and Soil Sensors, a volume in the Nanotechnology in the Agri-Food Industry series, is a practical resource that demonstrates how nanotechnology is a highly attractive tool that offers new options for the formulation of ‘nanopesticides’. Recent advances in nanopesticide research is reviewed and divided into several themes, including improvement of the water solubility of poorly soluble pesticide active ingredients to improve bioavailability and the encapsulation of pesticide active ingredients within permeable nanoparticles with the aim of releasing pesticide active ingredients in a controlled or targeted manner, while also protecting active ingredients from premature photo-degradation. Provides examples of pesticide formulations that contain inorganic and organic nanoparticles Includes general principles and the most recent applications of chemical sensors and multisensory systems for the assessment of soils and main soil nutrition component detection Presents the main benefits and drawbacks of chemical sensors and their employment in soil analysis for further applications Describes current issues of pesticide use, environmental contamination, bioaccumulation, and increases in pest resistance which demands a reduction in the quantity of pesticides applied for crop and stored product protection
Author : Adil Denizli
Publisher : Elsevier
ISBN 13 : 0323852939
Total Pages : 825 pages
Book Rating : 4.3/5 (238 download)
Download or read book Nanosensors for Smart Agriculture written by Adil Denizli and published by Elsevier. This book was released on 2021-11-28 with total page 825 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanosensors for Smart Agriculture covers new breakthroughs in smart agriculture, highlighting new technologies, such as the internet of things, big data and artificial intelligence. In addition, the book provides the many advantages of nanosensors over their micro counterparts, such as lower power consumption, higher sensitivity, lower concentration of analytes, and smaller interaction distances between the object and sensor. Sections provide information on fundamental design concepts and emerging applications of nanosensors in smart agriculture. The book highlights how, when cultivating soil, nanosensors and their wireless networks can be used for soil quality monitoring (moisture/herbicides/organic compound/trace metals monitoring in soil, etc. Other applications cover how smart nanosensors can be used for virus detection and hygiene/pathogen controls in livestocks, their use as active transport tracking devices for smart tracking and tracing, and other various applications, such as (i) nanochips for identity (radio frequency identification), (ii) food inspection, (iii) intelligent food packaging, and (iv) smart storage. This is an important reference source for materials scientists and agricultural engineers who are looking to understand more about how nanosensor technology can be used to create more efficient and sustainable agricultural systems. Outlines the fabrication and fundamental design concepts of nanosensors for agricultural applications Explains how nanosensors are being used throughout the agricultural cycle – from crop growth to food manufacturing Assesses major challenges surrounding the application of nanosensors to agricultural applications in mass scale
Author : Bambang Kuswandi
Publisher : CRC Press
ISBN 13 : 1000170594
Total Pages : 272 pages
Book Rating : 4.0/5 (1 download)
Download or read book Sensor-Based Quality Assessment Systems for Fruits and Vegetables written by Bambang Kuswandi and published by CRC Press. This book was released on 2020-12-17 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here is an abundance of valuable information on different sensing techniques for fruits and vegetables. The volume covers emerging technologies, such as NMR, MRI, wireless sensor networks (WSN), and radio-frequency identification (RFID) and their potential for industrial applications. Key features of the volume: • Provides an inclusive review of the developments of sensors for quality analysis and inspection of fresh fruits and vegetables • Fosters an understanding of the basic sensing techniques for quality assessment of fresh fruits and vegetables • Covers advanced sensing technologies, including computer vision, spectroscopy, X-rays, magnetic resonance, mechanical contact, wireless sensor networks, and radio-frequency identification sensors • Reviews the significant progress in sensor development of noninvasive techniques for quality assessment of fruits and vegetables
Author : Toonika Rinken
Publisher : BoD – Books on Demand
ISBN 13 : 9535110357
Total Pages : 326 pages
Book Rating : 4.5/5 (351 download)
Download or read book State of the Art in Biosensors written by Toonika Rinken and published by BoD – Books on Demand. This book was released on 2013-03-13 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: The main challenge in biosensor development is their application for various practical tasks to provide a continuous and reliable flow of information about the indicators of natural and industrial processes and the surroundings, so enabling adequate feedback and control. Biosensors can provide essential information, as the quality of life depends mainly on our knowledge about what we breathe, what we eat and how our bodies are able to metabolize the material, which we contact. This book includes 14 chapters, written by 52 authors and is focused on the applications of biosensors for monitoring the parameters of environment, the quality of food and biomarkers of health.
Author : Yichi Su
Publisher :
ISBN 13 :
Total Pages : 161 pages
Book Rating : 4.:/5 (18 download)
Download or read book Development of Riboswitch-based Sensors for High-throughput Enzyme Activity Screens written by Yichi Su and published by . This book was released on 2018 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of robust assay reagents for high-throughput enzyme activity screens is essential for biomedical research and drug development. However, conventional protein-based assay reagents have their limitations, including difficulty in engineering, limited signal dynamic range, lack of small molecule specificity and batch-to-batch variation. Most importantly, few assay reagents are applicable to both in vitro and live cell settings. An attractive alternative that overcomes these limitations is riboswitch-based sensors. Riboswitches are cis-regulatory structured RNA elements capable of controlling expression of downstream genes by binding to small molecule ligands. The naturally evolved RNA elements retain the advantages of synthetic aptamers, including ease of engineering, low cost in synthesis, and high batch-to-batch reproducibility, while they incorporate additional desirable features including remarkable affinity and selectivity for small molecule ligands, high-fidelity folding and thermostability for functioning in cellular environment. One strategy to engineer riboswitch-based sensor is to fuse the canonical terminal stem of the riboswitch aptamer domain to an internal stem of a signaling aptamer. Using this strategy, several prototypical riboswitch-based sensors were developed to detect essential metabolites and second messengers both in vitro and in live bacteria. However, despite their great potential, the universality of riboswitch-based sensors is limited by the number and the diverse topologies of natural riboswitch classes. Herein, novel strategies for engineering riboswitch-based sensors for more diverse ligands with improved sensitivity and specificity are described. First, in order to engineer sensors based on pseudoknot-containing riboswitches, to which the conventional engineering strategy was not applicable, an alternative inverted fusion strategy was invented. Riboswitch-based fluorescent biosensors for an essential metabolite S-adenosyl-L-homocysteine(SAH) were developed using this strategy. These SAH biosensors provide a direct “mix and go” high-throughput activity assay for methyltransferases, and a way to monitor the increase of SAH levels in live Escherichia coli upon chemical inhibition of methylthioadenosine nucleosidase (MTAN), an enzyme that is involved in quorum-sensing. Then, in order to engineer riboswitch-based sensors to interrogate ligands that are structural analogues to natural riboswitch ligands, a pipeline involving phylogenetic screening and structure-guided rational design was demonstrated on engineering bacterial 3'3'-cyclic di-GMP (cdiG) riboswitch-based biosensors into sensing a mammalian immune pathway second messenger 2'3'-cyclic-AMP-GMP (cGAMP). The resulting biosensors enabled in vitro high-throughput activity screening assays for cGAMP synthase, and direct cGAMP quantitation in dsDNA-stimulated L929 cell lysates. Finally, aiming to further expand the target ligand scope of riboswitch-based sensors to virtually any given small molecule ligands, two novel mechanism-based in vitro selection strategies, termed Capture-SELEX and Dye-SELEX, were explored. Proof-of-concept experiments showcased their capability of enriching sequences with desirable sensing properties from random RNA counterparts. It is envisioned that the strategies described here will forward the development of new riboswitch-based sensors as competitive assay reagents to antibodies, and eventually advancing basic biological understanding and biomedical applications.
Author : Ashok Mulchandani
Publisher : Humana Press
ISBN 13 : 9781617370281
Total Pages : 264 pages
Book Rating : 4.3/5 (72 download)
Download or read book Enzyme and Microbial Biosensors written by Ashok Mulchandani and published by Humana Press. This book was released on 2010-11-19 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1962 Clark and Lyons pioneered the concept of a biosensor. They p- posed immobilizing enzymes at electrochemical detectors to form “enzyme el- trodes” in order to expand the analyte range of ther base sensor. Smce then, the field of blosensors has greatly expanded. Some of the reasons for the expansion include both advances in signal transduction technologies and the incorporation of different biological sensing elements (Table 1). As a consequence, there are now a bewildering array of permutations of the biological sensing element and signal transducers that can be used to c- struct a biosensor. The purpose of the two volumes of Protocols and Te- niques in Biosensors is to provide a basic reference tool and starting point for use by graduate students, postdoctoral and senior researchers, and technicians m academics, industry, and government research establishments, to enable rapid entry into the field of biosensors. There are a variety of approaches that researchers employ to select a combination of bioaffinity elements and signal transducers. One commonly used approach is to identtfy the compound or compounds of interest; identify the biological molecule that yields an appropriate recognitionlselectivtty and dynamic concentration range for the assay; and choose an assay format and signal transduction technology that will meet the analytical requirements for the proposed application, This volume, Enzyme and Microbial Biosensors: Techniques and Protocols, describes a variety of transduction technologies that have been interfaced to enzymes and microorganisms.
Author : Alagarsamy Pandikumar
Publisher : Elsevier
ISBN 13 : 0128156392
Total Pages : 364 pages
Book Rating : 4.1/5 (281 download)
Download or read book Graphene-Based Electrochemical Sensors for Biomolecules written by Alagarsamy Pandikumar and published by Elsevier. This book was released on 2018-10-22 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: Graphene-Based Electrochemical Sensors for Biomolecules presents the latest on these nanomaterials that have gained a lot of attention based on their unique properties of high mechanical flexibility, large surface area, chemical stability, superior electric and thermal conductivities that render them great choices as alternative electrode materials for electrochemical energy storage and sensor applications. The hybridization of graphene with other nanomaterials induces a synergetic effect, leading to the improvement in electrical conductivity, stability and an enhancement of the electrocatalytic activity of the new nanocomposite material. This book discusses the electrochemical determination of a variety of biomolecules using graphene-based nanocomposite materials. Finally, recent progress in the development of electrochemical sensors using graphene-based nanocomposite materials and perspectives on future opportunities in sensor research and development are discussed in detail. Covers the importance of detecting biomolecules and the application of graphene and its nanocomposite materials in the detection of a wide variety of bioanalytes Presents easily understood fundamentals of electrochemical sensing systems and the role of graphene-based nanocomposite materials in research and development
Author : Sangita Das
Publisher : John Wiley & Sons
ISBN 13 : 3527846689
Total Pages : 1409 pages
Book Rating : 4.5/5 (278 download)
Download or read book Organic and Inorganic Materials Based Sensors, 3 Volumes written by Sangita Das and published by John Wiley & Sons. This book was released on 2023-12-22 with total page 1409 pages. Available in PDF, EPUB and Kindle. Book excerpt: Organic and Inorganic Materials Based Sensors A three-volume comprehensive overview of the development and applications of various novel potent molecular sensor frameworks In Organic and Inorganic Materials Based Sensors (3 Volume Set), a team of distinguished researchers delivers an interdisciplinary presentation of the engineering of high-performance biopolymer-based bio-nanocomposites, as well as strategies for the use of various molecules in the detection of environmentally important guest analytes. This three-volume book explores the most relevant technological developments in nanomaterials sensors and offers a broad and comprehensive overview of cutting-edge research on advanced materials in the fast-moving sensors industry. The authors explain the science behind nanomaterials for environmental remediation as well as the components and ingredients of the relevant materials. Readers will also find: Thorough introductions to sensory devices, polymer-based nano-biomaterials, and opto-electrochemical devices Comprehensive explorations of metal–organic frameworks, organic sensors, and organic–inorganic composite semiconductor sensors Practical discussions of vapochromic and vapoluminescent sensors Fulsome treatments of sensor ecosystems for health self-monitoring, including discussions of diabetes management Perfect for materials scientists, mechanical engineers, and analytical chemists, Organic and Inorganic Materials Based Sensors will also benefit inorganic and organic chemists, robotics engineers, and professionals working in the sensor industry.
Author : Serge Cosnier
Publisher : CRC Press
ISBN 13 : 9814411477
Total Pages : 405 pages
Book Rating : 4.8/5 (144 download)
Download or read book Electrochemical Biosensors written by Serge Cosnier and published by CRC Press. This book was released on 2015-01-26 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analy
Author : Anthony P. F. Turner
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 792 pages
Book Rating : 4.3/5 (91 download)
Download or read book Biosensors written by Anthony P. F. Turner and published by Oxford University Press, USA. This book was released on 1989 with total page 792 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first comprehensive book to be published in this field. It has many contributors, chosen to reflect the spread of disciplines from which the new techniques have emerged.
