Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
The Effects Of Over Expressing The Proteins Of The Dnaa Operon In Wild Type And Mutant Escherichia Coli Cells
Download The Effects Of Over Expressing The Proteins Of The Dnaa Operon In Wild Type And Mutant Escherichia Coli Cells full books in PDF, epub, and Kindle. Read online The Effects Of Over Expressing The Proteins Of The Dnaa Operon In Wild Type And Mutant Escherichia Coli Cells ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis The Effects of Over-expressing the Proteins of the DnaA Operon in Wild-type and Mutant Escherichia Coli Cells by : Aline V. Grigorian
Download or read book The Effects of Over-expressing the Proteins of the DnaA Operon in Wild-type and Mutant Escherichia Coli Cells written by Aline V. Grigorian and published by . This book was released on 2002 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of the DnaA Promoter in Escherichia Coli by : Victoria Goehner Newman
Download or read book Regulation of the DnaA Promoter in Escherichia Coli written by Victoria Goehner Newman and published by . This book was released on 1999 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: The timing of initiation of DNA replication from oriC in E. coli is regulated by the concentration of the DnaA protein. Increases in the intracellular DnaA protein concentration over 1.5 fold normal levels causes asynchronous timing of initiation of DNA replication. The regulators of the dnaA operon must keep the DnaA concentration within tight limits yet be able to respond to rapidly changing environmental conditions. Here it is shown that Fis and DnaA proteins interact, in conjunction with other factors, to regulate the expression of dnaA. Binding of DnaA to the DnaA box in the dnaA promoter region is destabilized by the binding of Fis to its binding site in dnaAp2. However, DnaA94, containing only the C-terminal 94 amino acids of the DnaA protein and retaining specific binding activity to the DnaA box of the dnaA promoter region, is not destabilized by Fis binding. DNase I and copper-phenanthroline (Cu-op) footprinting studies of DnaA and DnaA94 support the existence of a second weak DnaA box which abuts the DNase I and Cu-op footprints of Fis in the dnaAp2 promoter. We determined that the GC-rich region of the dnaAp2 promoter is analogous to the discriminator sequence (DS) of rRNA promoters, in that the GC-rich sequence mediates the repression caused by elevated levels of guanosine tetraphosphate (ppGpp). During starvation conditions, the alarmone ppGpp is synthesized. Overexpression of ppGpp causes inhibition of dnaAp2 promoter transcription, which is alleviated when the GC-rich DS is mutated to an AT-rich sequence. We conclude that this site is responsible for mediating the stringent control of the dnaAp2 promoter. Incorrect timing of initiation in the cell cycle results in asynchronous replication. Strains with a mutated DS, DnaA box, or Fis binding site in the dnaA promoter region on the chromosome have increased levels of dnaA gene expression. These mutants initiate DNA replication synchronously in minimal M9 medium as shown by flow cytometric assays. In rich medium, the DS, mutant showed very limited asynchrony, whereas the other mutants replicated synchronously.
Book Synopsis Bacterial Chromatin by : Remus T. Dame
Download or read book Bacterial Chromatin written by Remus T. Dame and published by Springer Nature. This book was released on with total page 654 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of E. Coli Nrd Operon Encoding Ribonucleotide Reductase by : Lei Sun
Download or read book Regulation of E. Coli Nrd Operon Encoding Ribonucleotide Reductase written by Lei Sun and published by . This book was released on 1993 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Effects of Isolated Missense Mutations from the DnaA5 and DnaA46 Alleles in Initiation of Escherichia Coli OriC Replication by : Kevin Michael Carr
Download or read book Effects of Isolated Missense Mutations from the DnaA5 and DnaA46 Alleles in Initiation of Escherichia Coli OriC Replication written by Kevin Michael Carr and published by . This book was released on 1994 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis An Unknown Regulator Affects Cell Division and the Timing of Entry Into Stationary Phase in Escherichia Coli by : Sherrie Valarie Bain
Download or read book An Unknown Regulator Affects Cell Division and the Timing of Entry Into Stationary Phase in Escherichia Coli written by Sherrie Valarie Bain and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: When an essential nutrient is depleted from the medium, cultures of wild-type E. coli cells enter a period called stationary phase. The transition into stationary phase is marked by distinct changes in cell physiology, gene expression, and morphology. Prüss and Matsumura (18) found a mutant strain of E. coli that was able to continue growing exponentially at a time when wild-type cells had stopped growing and entered stationary phase. They concluded that FlhD, a transcriptional activator of flagellar genes, was responsible for this growth phenotype and that it is a regulator of cell division (17, 18). Contrary to the findings of Prüss and Matsumura, research in our lab has shown that the mutant growth phenotype observed in the strain used by Matsumura and Prüss is flhD independent. This study sought to identify the second mutation, which we call cdr (cell division regulator) in the strain used by Matsumura and Prüss. We used Hfr mapping and P1 transduction to localize the mutation to a specific region of the chromosome. We also sought to determine if this growth phenotype was due to loss of function or gain of function and whether the mutation in the cdr gene was sufficient to cause the observed growth phenotype in other strain backgrounds. In addition the growth phenotype of these two strains was compared to that of other wild-type and standard laboratory E. coli strains. Our results indicate that the cdr mutation is located in the 88.5' region of the chromosome and is due to loss of Cdr function. We also discovered that the growth phenotype assigned to the mutant strain more closely reflects that of other wild-type laboratory strains as did the morphology of cells in stationary phase. This evidence suggests that the actual mutant strain might be the one that was designated as the wild-type strain by Matsumura and Prüss and both strains may contain mutations that actually cause a decrease in cell number instead of an increase as previously reported.
Book Synopsis The Interaction of Escherichia Coli Gene Products with Mutant Forms of DnaA Protein by : Theodore Robert Hupp
Download or read book The Interaction of Escherichia Coli Gene Products with Mutant Forms of DnaA Protein written by Theodore Robert Hupp and published by . This book was released on 1990 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Gene Expression in Escherichia Coli in Response to Oxygen, Nitrate and Global Regulators of Transcription by : Rebecca Sophia Natalie Krupp
Download or read book Analysis of Gene Expression in Escherichia Coli in Response to Oxygen, Nitrate and Global Regulators of Transcription written by Rebecca Sophia Natalie Krupp and published by . This book was released on 2006 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Effect of Growth Rate on the Expression of Escherichia Coli DnaA Protein by : Sandra Ann Putirka
Download or read book The Effect of Growth Rate on the Expression of Escherichia Coli DnaA Protein written by Sandra Ann Putirka and published by . This book was released on 1992 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :Errol C. Friedberg Publisher :American Society for Microbiology Press ISBN 13 :1555813194 Total Pages :2587 pages Book Rating :4.5/5 (558 download)
Book Synopsis DNA Repair and Mutagenesis by : Errol C. Friedberg
Download or read book DNA Repair and Mutagenesis written by Errol C. Friedberg and published by American Society for Microbiology Press. This book was released on 2005-11-22 with total page 2587 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential resource for all scientists researching cellular responses to DNA damage. • Introduces important new material reflective of the major changes and developments that have occurred in the field over the last decade. • Discussed the field within a strong historical framework, and all aspects of biological responses to DNA damage are detailed. • Provides information on covering sources and consequences of DNA damage; correcting altered bases in DNA: DNA repair; DNA damage tolerance and mutagenesis; regulatory responses to DNA damage in eukaryotes; and disease states associated with defective biological responses to DNA damage.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1850 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Prokaryotic Gene Expression by : Simon Baumberg
Download or read book Prokaryotic Gene Expression written by Simon Baumberg and published by OUP Oxford. This book was released on 1999-05-27 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prokaryotic gene expression is not only of theoretical interest but also of highly practical significance. It has implications for other biological problems, such as developmental biology and cancer, brings insights into genetic engineering and expression systems, and has consequences for important aspects of applied research. For example, the molecular basis of bacterial pathogenicity has implications for new antibiotics and in crop development. Prokaryotic Gene Expression is a major review of the subject, providing up-to-date coverage as well as numerous insights by the prestigious authors. Topics covered include operons; protein recognition of sequence specific DNA- and RNA-binding sites; promoters; sigma factors, and variant tRNA polymerases; repressors and activators; post-transcriptional control and attenuation; ribonuclease activity, mRNA stability, and translational repression; prokaryotic DNA topology, topoisomerases, and gene expression; regulatory networks, regulatory cascades and signal transduction; phosphotransfer reactions; switch systems, transcriptional and translational modulation, methylation, and recombination mechanisms; pathogenicity, toxin regulation and virulence determinants; sporulation and genetic regulation of antibiotic production; origins of regulatory molecules, selective pressures and evolution of prokaryotic regulatory mechanisms systems. Over 1100 references to the primary literature are cited. Prokaryotic Gene Expression is a comprehensive and authoritative review of current knowledge and research in the area. It is essential reading for postgraduates and researchers in the field. Advanced undergraduates in biochemistry, molecular biology, and microbiology will also find this book useful.
Download or read book Ebook: Biology written by BROOKER and published by McGraw Hill. This book was released on 2014-09-16 with total page 1423 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ebook: Biology
Download or read book Index Medicus written by and published by . This book was released on 2003 with total page 2214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Book Synopsis Selected Topics in DNA Repair by : Clark Chen
Download or read book Selected Topics in DNA Repair written by Clark Chen and published by BoD – Books on Demand. This book was released on 2011-10-26 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended for students and scientists working in the field of DNA repair, focusing on a number of topics ranging from DNA damaging agents and mechanistic insights to methods in DNA repair and insights into therapeutic strategies. These topics demonstrate how scientific ideas are developed, tested, dialogued, and matured as it is meant to discuss key concepts in DNA repair. The book should serve as a supplementary text in courses and seminars as well as a general reference for biologists with an interest in DNA repair.
Book Synopsis The Role of DNA Repair Pathways in Resistance to Chemotherapy and Radiotherapy in Cancer by : José Díaz-Chávez
Download or read book The Role of DNA Repair Pathways in Resistance to Chemotherapy and Radiotherapy in Cancer written by José Díaz-Chávez and published by Frontiers Media SA. This book was released on 2022-04-29 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis From Genome to Proteome by : Michael J. Dunn
Download or read book From Genome to Proteome written by Michael J. Dunn and published by John Wiley & Sons. This book was released on 2008-11-21 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: As research on the human, animal, plant and microbial genomes matures towards descriptive fullness, the need for understanding the proteome has clearly emerged as the next major endeavor of life sciences. Proteomics - the quantitative analysis of all proteins working in a cell at a specific time and at specific conditions - provides deep insight into the highly organized network of expression, modification and degradation of proteins. Compiled in this book are reviews and research articles which describe the recent advances and perspectives of this new field of research. The articles are grouped into the following sections: - Sample Preparation and Solubilization - Developments in Electrophoresis - Detection and Quantitation - Mass Spectrometry - Proteome Data Analysis and Management - Prokayotes and Yeast - Biological Fluids - Eukaryotic Cells and Tissue - Oncology - Plants Proteomics is a new key for the functional analysis of living systems and of equal importance for basic as well as application oriented research.
