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The Development Of A Microchip Based Capillary Electrophoresis Device With Sinusoidal Voltammetric Detection For The Analysis Of Biomolecules
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Book Synopsis The Development of a Microchip-based Capillary Electrophoresis Device with Sinusoidal Voltammetric Detection for the Analysis of Biomolecules by : Nicole Eleanor Hebert
Download or read book The Development of a Microchip-based Capillary Electrophoresis Device with Sinusoidal Voltammetric Detection for the Analysis of Biomolecules written by Nicole Eleanor Hebert and published by . This book was released on 2003 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis and Microchip Capillary Electrophoresis by : Carlos D. García
Download or read book Capillary Electrophoresis and Microchip Capillary Electrophoresis written by Carlos D. García and published by John Wiley & Sons. This book was released on 2013-02-01 with total page 638 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explores the benefits and limitations of the latest capillary electrophoresis techniques Capillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization. With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample. The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include: Critical evaluation of the use of surfactants in capillary electrophoresis Sampling and quantitative analysis in capillary electrophoresis Capillary electrophoresis with electrochemical detection Overcoming challenges in using microchip electrophoresis for extended monitoring applications Capillary electrophoresis of intact unfractionated heparin and related impurities Microchip capillary electrophoresis for in situ planetary exploration Each chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications.
Book Synopsis Microchip Capillary Electrophoresis by : Charles Henry
Download or read book Microchip Capillary Electrophoresis written by Charles Henry and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.
Book Synopsis Microfluidic Chip-Capillary Electrophoresis Devices by : Ying Sing Fung
Download or read book Microfluidic Chip-Capillary Electrophoresis Devices written by Ying Sing Fung and published by CRC Press. This book was released on 2015-08-18 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) and microfluidic chip (MC) devices are relatively mature technologies, but this book demonstrates how they can be integrated into a single, revolutionary device that can provide on-site analysis of samples when laboratory services are unavailable. By introducing the combination of CE and MC technology, Microfluidic Ch
Book Synopsis Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques by : James P. Landers
Download or read book Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques written by James P. Landers and published by CRC Press. This book was released on 2007-12-18 with total page 1598 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.
Book Synopsis Microchip Capillary Electrophoresis Protocols by : Ann Van Schepdael
Download or read book Microchip Capillary Electrophoresis Protocols written by Ann Van Schepdael and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis. Chapters focus on small molecule, biomolecule applications, various detection modes, and sample preparation approaches are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microchip Capillary Electrophoresis Protocol aids scientists in continuing to study microchip capillary electrophoresis.
Book Synopsis Encyclopedia of Microfluidics and Nanofluidics by : Dongqing Li
Download or read book Encyclopedia of Microfluidics and Nanofluidics written by Dongqing Li and published by Springer Science & Business Media. This book was released on 2008-08-06 with total page 2242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering all aspects of transport phenomena on the nano- and micro-scale, this encyclopedia features over 750 entries in three alphabetically-arranged volumes including the most up-to-date research, insights, and applied techniques across all areas. Coverage includes electrical double-layers, optofluidics, DNC lab-on-a-chip, nanosensors, and more.
Book Synopsis Microchip-Capillary Electrophoresis Devices with Dual-Electrode Detectors for Determination of Polyphenols, Amino Acids and Metabolites in Wine and Biofluids by : Fuying Du
Download or read book Microchip-Capillary Electrophoresis Devices with Dual-Electrode Detectors for Determination of Polyphenols, Amino Acids and Metabolites in Wine and Biofluids written by Fuying Du and published by Open Dissertation Press. This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Microchip-capillary Electrophoresis Devices With Dual-electrode Detectors for Determination of Polyphenols, Amino Acids and Metabolites in Wine and Biofluids" by Fuying, Du, 杜富滢, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: The electrochemical detector provides a promising detection mode for capillary electrophoresis (CE) due to its excellent sensitivity, good portability, high selectivity, easy miniaturization, low capital and running cost. To widen its scope for determining trace analytes in complex samples, three dual-electrode detectors were fabricated to enable the determination of electro-inactive analytes, to assess co-eluted peaks and to give a large enhancement of the detection sensitivity by modifying electrode surface using multi-walled carbon nanotubes (MWNTs). To determine trace non-electroactive amino acids present in human tears, a serial dual-electrode detector was developed using an upstream on-capillary Pt film electrode to oxidize bromide to bromine at +1.0 V and a downstream Pt disk electrode to detect the residual bromine at +0.2 V after their reaction with amino acids eluted out from the separation capillary. The bromide reagent was introduced after CE separation by a newly designed coaxial post-column reactor fabricated onto the PMMA chip. Using optimized CE buffer containing 20 mM borate, 20 mM SDS at pH 9.8, L-glutamine, L-alanine and taurine were baseline separated with detection limits ranging from 0.56-0.65 μM and a working range of 2-200 μM for L-glutamine and of 2-300 μM for both L-alanine and taurine. Method reliability was established by close to 100% recoveries for spiked amino acids and good agreement between the measured and the literature reported amino acid concentrations in tears. For the determination of polyphenols in wine, a microchip-CE device was fabricated with a dual-opposite carbon fiber microelectrode operated in a parallel mode to assess peak purity. Under optimized conditions, (+)-catechin, trans-resveratrol, quercetin, (-)-epicatechin and gallic acid were baseline separated within 16 min with detection limits ranging from 0.031- 0.21 mg/L and repeatability of 2.0-3.3 % (n=5). The use of an opposite dual-electrode enables the simultaneous determination of peaks and measurement of their current ratios at +0.8 V and +1.0 V vs Ag/AgCl. The capability of using current ratio to identify the presence of co-migrating impurities was demonstrated in a mixed standard solution with overlapping (+)-catechin and (-)-epicatechin peaks and in a commercial red wine with interfering impurities. Matching of both the migration time and the current ratio reduce false positive and validate polyphenol quantitation in red wine. Lastly, a dual-opposite MWNTs modified carbon fiber microelectrode (CFME) was developed to determine the biomarkers (4-nitrophenol, 4-nitrophenyl-glucuronide and 4-nitrophenyl-sulfate) needed to assess exposure to methyl parathion. Use of the MWNTs modified CFME showed a much higher sensitivity than bare CFME, with a detection limit of 0.46 μM for 4-nitrophenol. Baseline separation of all three biomarkers was obtained within 31 min by a 45 cm long capillary under 12 kV in a 20 mM phosphate buffer at pH 7.0. The method developed was successfully utilized to determine low levels of biomarkers in human urine without using complex pretreatment steps and delivered recoveries ranging from 95.3 - 97.3% and RSDs within 5.8% (n=3). Using a parallel dual-electrode detector was shown to deliver reliable results with matching current ratios and comparable migration ti
Book Synopsis Microchip Capillary Electrophoresis by : Maojun Gong
Download or read book Microchip Capillary Electrophoresis written by Maojun Gong and published by . This book was released on 2006 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microchip capillary electrophoresis (CE) is the miniaturized form of traditional CE, a powerful electrical separation technique performed in a capillary with micron diameters. The advantages of microchip CE over traditional CE include shorter analysis time and less consumption of chemicals and reagent. The objective of the microchip program in our group is to develop microchip CE instrumentation and methodologies for high throughput screening of pharmaceuticals. As an important step, my work was focused on the development of methodologies for binding studies and trace protein assays in single-lane glass microchips. A pair of thrombin and thrombin-binding aptamer was chosen as a model system to demonstrate the feasibility of microchip CE in binding studies. The combination of a short detection length (1.0 cm) and a high electric field (670 V/cm) succeeded in the detection of the aptamer-thrombin complex and reduction of thrombin adsorption. The limit of detection (LOD) reached 5.0 nM of thrombin. To determine the equilibrium dissociation constant of aptamer-thrombin, a simple hydrodynamic injection method in microchips was developed. This hydrodynamic injection significantly reduces sample bias although the dispensing bias still exists. Using the hydrodynamic injection method, the dissociation constant of aptamer-thrombin was determined to be 23 nM which is consistent with reported results. Moreover, an accurate and fast frontal analysis method was developed for the determination of dissociation constant with a model pair of aptamer and immunoglobulin E (IgE). The capability of microchip CE coupled with a laser-induced fluorescence (LIF) detection system in the detection of trace protein was demonstrated using a 66-mer photoaptamer to detect 165-mer vascular endothelial growth factor (VEGF165). A LOD of 1.0 nM was achieved for the detection of VEGF165 using the fluorescently-labeled photoaptamer. To enhance detection sensitivity of analyte in microchip CE, a field-amplified stacking injection (FASI) was developed in a single-lane glass microchip. This FASI method takes advantage of the pressure-induced flow as a way to supply analytes for stacking. A 100-fold detection enhancement for model analytes of fluorescein (FL), FITC and 5-carboxyfluorescein (5-FAM) using FASI was achieved relative to the commonly used pinched injection method. Sweeping is an effective and convenient way to preconcentrate analytes in micellar electrokinetic chromatography (MEKC). Several sweeping schemes in traditional and microchip CE were developed using cationic surfactants. A new sweeping carrier, aggregates of dodecyltrimethylammonium bromide (DTAB), was found to have strong sweeping power for oligonucleotides at concentrations below, instead of above, the critical micelle concentration (CMC). A 112-fold detection enhancement was achieved for 15-mer thrombin-binding aptamer in traditional CE. An unlimited-volume injection method was developed using electrokinetic stacking injection (EKSI). Equivalent injection capillary lengths of 7.3 and 8.4 m for FL and 5-FAM were achieved in 60-min EKSI using DTAB micelles. A flow control method for sweeping microchip CE was developed using tetradecyltrimethylammonium bromide (TTAB). This method successfully preconcentrated analytes of FL and 5-FAM with detection enhancements of 250- and 500-fold, respectively.
Book Synopsis Clinical Applications of Capillary Electrophoresis by : Stephen M. Palfrey
Download or read book Clinical Applications of Capillary Electrophoresis written by Stephen M. Palfrey and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.
Book Synopsis Development of an Optical MEMS-based Detection Scheme for Microchip Capillary Electrophoresis by : Kurt D. Kramer
Download or read book Development of an Optical MEMS-based Detection Scheme for Microchip Capillary Electrophoresis written by Kurt D. Kramer and published by . This book was released on 1998 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Carbohydrates by Capillary Electrophoresis by : Aran Paulus
Download or read book Analysis of Carbohydrates by Capillary Electrophoresis written by Aran Paulus and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wir sind umgeben von Kohlenhydraten: der süße Kaffee, Tee oder Dessert, die Stärke als Hauptkomponente unserer Nahrung und die Zellulose als Strukturelement in Pflanzen. Kohlenhydrate sind eine wichtige Klasse biologischer Moleküle, die an einer Anzahl wichtiger biochemischer Prozesse beteiligt sind. Gerade beginnen wir die Rolle von komplexen Zuckern zu verstehen, die an Proteine gebunden die Kommunikation von Zellen in einer "Zuckersprache" bewerkstelligen. Und nicht zuletzt kommen die ersten Kohlenhydratmoleküle als Medikamente auf den Markt. Anders als für andere Biopolymere sind die analytischen Methoden zur strukturellen Charakterisierung und Sequenzanalyse für Kohlenhydrate zur Zeit ungenügend, zum Teil wegen der überwältigenden Isotopenzahl der Zucker. Dieses Buch beschreibt die Entwicklung der letzten Jahre, die mit der Kapillarelektrophorese in Bezug auf eine miniaturisierte Analytik mit besserer Auflösung und Empfindlichkeit gemacht wurden. Instrumentierung, Derivatisierung, Trennbedingungen und Anwendungen in verschiedenen Disziplinen und Industrien wie z. B. Glykobiologie, Lebensmittelindustrie und Biotechnologie werden beschrieben.
Book Synopsis Development of Capillary Electrophoresis-based Microfluidic Devices for Future Medical Diagnostics by : Abdulilah Abdulqader Dawoud
Download or read book Development of Capillary Electrophoresis-based Microfluidic Devices for Future Medical Diagnostics written by Abdulilah Abdulqader Dawoud and published by . This book was released on 2006 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genotoxic carcinogens can react covalently with DNA to form DNA adducts, which can lead to mutations in critical genes and subsequently to the development of diseases such as cancer. Consequently, the role of DNA damage and their subsequent biomarkers are considered important in studies involving cancer and other aging-related diseases. Analytical methods with high resolving power and sensitive detection are needed to detect neurotransmitters (e.g. dopamine (DA) and DA-derived DNA adduct), catechol estrogen-derived DNA adducts, and 8-hydroxy-deoxyguanosine DNA adduct in human fluids, as their presence is difficult to determine by standard chromatography with UV absorbance detection. Furthermore, these DNA adducts are weakly fluorescent at room temperature. Thus, native fluorescence detection methods are ineffective with this class of biomarkers. Interestingly, capillary electrophoresis (CE) combined with electrochemical detection (ED) has been found to be a sensitive, selective technique for analyzing a large number of compounds including these analytes. Therefore, a CE-based PDMS/glass hybrid microfluidic device for free solution electrophoresis with totally integrated gold electrodes and simplified gated sample injection were fabricated to separate and detect various DNA adducts and other related biomarkers. Various arrangements of the ED system were tested and evaluated including in-channel and end-channel detection. The best electrode configuration was optimized based on in-channel detection with an in situ fabricated palladium decoupler serving as the electrophoretic ground located inside the microchannel. With these microdevices, excellent separation of the above analytes can be accomplished in relatively short times (
Book Synopsis Capillary Electrophoresis Technology by : Norberto A. Guzman
Download or read book Capillary Electrophoresis Technology written by Norberto A. Guzman and published by CRC Press. This book was released on 1993-08-10 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tracing the sequence of observations that has led to current understanding in the field, this reference presents the basic concepts, instrumentation and applications of capillary electrophoresis, examining its many features such as high-power resolution, high-mass sensitivity, overall sensitivity and low-sample volume requirements. This work highlights the use of capillary elctrophoresis for the identification, separation, detection and characterization of substances on the molecular counting level. Illustrating the major technical maneuvers for common operations and applications, Capillary Electrophoresis Technology outlines the theoretical concepts and mathematical expressions of capillary electrophoresis; describes advances in instrumentation hardware and detection systems. It explains the advantages and limitations of the different variants of capillary electrophoresis; and provides extra coverage of areas in which capillary electrophoresis has grown increasingly popular, including the identification and characterization of small molecules and macromolecules. Written by experts in the field, this book is aimed at analytical and clinical chemists and biochemists, chemical engineers, biologists, pharmacists, biotechnologists and students in these disciplines.
Author :Philippe Schmitt-Kopplin Publisher :Springer Science & Business Media ISBN 13 :1588295397 Total Pages :808 pages Book Rating :4.5/5 (882 download)
Book Synopsis Capillary Electrophoresis by : Philippe Schmitt-Kopplin
Download or read book Capillary Electrophoresis written by Philippe Schmitt-Kopplin and published by Springer Science & Business Media. This book was released on 2008-02-25 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics.
Author :Jiang, Yutao Publisher :National Library of Canada = Bibliothèque nationale du Canada ISBN 13 :9780612400641 Total Pages :148 pages Book Rating :4.4/5 (6 download)
Book Synopsis A Multi-reflection Cell for Enhanced Absorbance Detection in Microchip-based Capillary Electrophoresis Devices [microform] by : Jiang, Yutao
Download or read book A Multi-reflection Cell for Enhanced Absorbance Detection in Microchip-based Capillary Electrophoresis Devices [microform] written by Jiang, Yutao and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1999 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition by : James P. Landers
Download or read book Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition written by James P. Landers and published by CRC Press. This book was released on 2007-12-18 with total page 1592 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. While the body of international CE literature continues to expand dramatically, experts still question whether it has provided the speed, resolving power, peak capacity, sensitivity, robustness, and cost-reduction promised by its pioneers. Responding to these criticisms, this third edition brings together cutting-edge researchers to demonstrate the utility of CE across a broad spectrum of disciplines including— Forensic science Medical diagnostics Pharmaceutical science Genetic analysis Biotechnology Fluid mechanics Environmental science Biomedical research Nanotechnology Proteomics Detailed Analysis of New Methodologies and Applications Eagerly awaited by researchers and technicians who transformed the first two editions into bestsellers, this latest volume once again delivers. Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Aided by contributions from leading international experts, this text remains a seminal reference for numerous chemistry, biology, and engineering fields.
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