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The Contribution Of Saccharomyces Cerevisiae Cap Binding Complex To Small Nuclear And Small Nucleolar Rna Processing
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Book Synopsis The Contribution of Saccharomyces Cerevisiae Cap Binding Complex to Small Nuclear and Small Nucleolar RNA Processing by : Sylwia Szczepaniak
Download or read book The Contribution of Saccharomyces Cerevisiae Cap Binding Complex to Small Nuclear and Small Nucleolar RNA Processing written by Sylwia Szczepaniak and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Słowa kluczowe: sn/snoRNA RNA maturation, 5' cap, 3' processing, CBC, NNS, Rnt1.
Book Synopsis The Saccharomyces Cerevisiae Nuclear Cap Binding Complex Regulates RNA Synthesis and Processing Through Interactions with RNA Polymerase II by : Christina Chung (Ph. D.)
Download or read book The Saccharomyces Cerevisiae Nuclear Cap Binding Complex Regulates RNA Synthesis and Processing Through Interactions with RNA Polymerase II written by Christina Chung (Ph. D.) and published by . This book was released on 2009 with total page 53 pages. Available in PDF, EPUB and Kindle. Book excerpt: The nuclear Cap Binding Complex (CBC), which consists of a large subunit CBP80 and a small subunit CBP20, is structurally conserved from yeast to mammals. The complex has been implicated in multiple steps in mRNA processing including mRNA stability, pre-mRNA splicing, and mRNA export. The research described here provides evidence that the CBC plays a novel role in transcription through interactions with the RNA polymerase II (RNAPII), particularly through the C-terminal domain of the largest subunit (CTD). These interactions appear at least partially RNA independent and occur during active transcription. Although the CBC does not possess kinase or phosphatase activity, it does appear to affect CTD phosphorylation. One effect of this CBC-CTD interaction is regulation of the Nrd1 termination complex activity in attenuation of the IMP dehydrogenase gene IMD2 and its constituent cryptic unstable transcripts (CUTs). The involvement of the CBC with the RNA from capping to export and with RNAPII places it in an ideal position to provide regulatory feedback from the nascent RNA and RNA processing factors to the transcriptional machinery.
Book Synopsis Characterization of Early Events in the Biogenesis of Box H/ACA Small Nucleolar Ribonucleoprotein Particles in Saccharomyces Cerevisiae by : Pok Kwan Yang
Download or read book Characterization of Early Events in the Biogenesis of Box H/ACA Small Nucleolar Ribonucleoprotein Particles in Saccharomyces Cerevisiae written by Pok Kwan Yang and published by . This book was released on 2005 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Exploration of the RNase III Dependent Nuclear Processing and Degradation Mechanisms of RNA in Saccharomyces Cerevisiae by : Defne Emel Egecioglu
Download or read book Exploration of the RNase III Dependent Nuclear Processing and Degradation Mechanisms of RNA in Saccharomyces Cerevisiae written by Defne Emel Egecioglu and published by . This book was released on 2009 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Demystifying the Role of the Cap Binding Complex (CBC) Within the Gene Expression Network in Saccharomyces Cerevisiae by : Julia McBride Claggett
Download or read book Demystifying the Role of the Cap Binding Complex (CBC) Within the Gene Expression Network in Saccharomyces Cerevisiae written by Julia McBride Claggett and published by . This book was released on 2011 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: The highly conserved Cap Binding Complex (CBC) is comprised of two subunits, Cbp20 and Cbp80, and binds to the 5' end of pre-mRNA as it emerges from the transcribing RNA polymerase (Pol II). Evidence suggesting a role for the CBC in multiple reactions during gene expression, including transcription, splicing, 3' end processing, and RNA export, prompted us to hypothesize that the CBC is involved in coupling these reactions, and that this coordinated regulation facilitates the ability of a cell to respond to stress. The research presented in this dissertation specifically dissects the roles of the CBC in splicing and in transcription and provides insight into a mechanism by which the CBC might contribute to the coupling of these two reactions in Saccharomyces cerevisiae. We have demonstrated that the CBC is specifically required for the splicing of two intron-containing genes with a particular non-consensus 5' splice site, SUS1 and GCR1. Through its effects on splicing, we found that the CBC contributes to the regulation of a histone modification and the acitivity of a key glycolytic transcription factor, resulting in genome-wide changes to gene expression when the CBC is deleted. We discovered that the CBC is down-regulated at multiple levels in response to nutrient stress, suggesting that the coordinated activities of the CBC are particularly important under these conditions. One of these levels of regulation appears to be proteolysis by the proteasome. Furthermore, a role for the CBC in repressing expression of Rpn4, a critical regulator of proteasome levels, suggests that the CBC is involved in a feedback loop in which its targeting to the proteasome is determined by its ability to influence gene expression. Directed mutagenesis of evolutionarily-conserved domains of Cbp80 allowed us to uncouple the functions of the CBC in gene expression revealing a role for the CBC in transcription that is independent of its ability to act in splicing. In sum, the findings presented here demystify the role of the CBC in gene expression by enhancing our understanding of the CBC's role in splicing, demonstrating novel functions for the CBC in transcription, identifying multiple mechanisms by which the CBC is regulated, and illustrating a requirement for the CBC in the cellular stress response.
Book Synopsis Investigating the Role of the Saccharomyces Cerevisiae Poly(A) Binding Protein in 5-cap Complex Formation in Vitro by : Jennifer Marie Blanchette
Download or read book Investigating the Role of the Saccharomyces Cerevisiae Poly(A) Binding Protein in 5-cap Complex Formation in Vitro written by Jennifer Marie Blanchette and published by . This book was released on 2000 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Eukaryotic RNases and Their Partners in RNA Degradation and Biogenesis by : Guillaume F. Chanfreau
Download or read book Eukaryotic RNases and Their Partners in RNA Degradation and Biogenesis written by Guillaume F. Chanfreau and published by Academic Press. This book was released on 2012 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Genomewide Analysis of RNA Processing in Saccharomyces Cerevisiae by : Tyson A. Clark
Download or read book Genomewide Analysis of RNA Processing in Saccharomyces Cerevisiae written by Tyson A. Clark and published by . This book was released on 2002 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Functional Characterisation of Ribosome Biogenesis Cofactors in (Saccharomyces Cerevisiae) by :
Download or read book Functional Characterisation of Ribosome Biogenesis Cofactors in (Saccharomyces Cerevisiae) written by and published by . This book was released on 2015 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: The biogenesis of eukaryotic ribosomes is a highly complex and dynamic process, which requires a multitude of cofactors. In Saccharomyces cerevisiae, approximately 200 proteins have been identified to function in this pathway, coordinating ribosomal protein assembly and processing of ribosomal RNA (rRNA) precursors. Furthermore, small nucleolar ribonucleoprotein complexes (snoRNPs) introduce numerous nucleotide modifications within the rRNAs, which are guided by basepairing of their RNA component (snoRNA) with the designated target pre-rRNA. SnoRNA binding and pre-rRNA processing involve st...
Book Synopsis The Biochemical Characterization of Saccharomyces Cerevisiae H/ACA Small Nucleolar Ribonucleoproteins by : Jessica Durand
Download or read book The Biochemical Characterization of Saccharomyces Cerevisiae H/ACA Small Nucleolar Ribonucleoproteins written by Jessica Durand and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribosome biogenesis is a crucial yet poorly understood and complex process in all cells. To date, most studies on eukaryotic ribosome biogenesis have relied on yeast genetics and whole cell analysis of ribosomal RNA processing. An early and critical step in ribosome biogenesis is the post-transcriptional modification of rRNA. Pseudouridylation is the most frequently occurring modification. Pseudouridylation is catalyzed by H/ACA small nucleolar ribonucleoproteins (snoRNPs) which are one of the two major classes of snoRNPs found within eukaryotes and archaea. H/ACA snoRNPs consist of four conserved core proteins Cbf5, Gar1, Nop10, and Nhp2 (eukaryotes), and a substrate specific H/ACA snoRNA. Mutations causing the rare inherited disease Dyskeratosis congenita are found in CBF5, NOP10, and NHP2. Here I report the purification of H/ACA protein Cbf5 in the presence of detergents. Additionally, I report initial in vitro RNA binding studies using Nhp2 and the snoRNA snR34 as well as the effects of Dyskeratosis congenita substitutions within Nhp2 on this interaction.
Book Synopsis Multiple Mechanisms Regulating Ribosome Biogenesis in Saccharomyces Cerevisiae by : Chrissie Young Lee
Download or read book Multiple Mechanisms Regulating Ribosome Biogenesis in Saccharomyces Cerevisiae written by Chrissie Young Lee and published by . This book was released on 2008 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis RNA Exosome by : Torben Heick Jensen
Download or read book RNA Exosome written by Torben Heick Jensen and published by Springer Science & Business Media. This book was released on 2011-06-29 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: The diversity of RNAs inside living cells is amazing. We have known of the more “classic” RNA species: mRNA, tRNA, rRNA, snRNA and snoRNA for some time now, but in a steady stream new types of molecules are being described as it is becoming clear that most of the genomic information of cells ends up in RNA. To deal with the enormous load of resulting RNA processing and degradation reactions, cells need adequate and efficient molecular machines. The RNA exosome is arising as a major facilitator to this effect. Structural and functional data gathered over the last decade have illustrated the biochemical importance of this multimeric complex and its many co-factors, revealing its enormous regulatory power. By gathering some of the most prominent researchers in the exosome field, it is the aim of this volume to introduce this fascinating protein complex as well as to give a timely and rich account of its many functions. The exosome was discovered more than a decade ago by Phil Mitchell and David Tollervey by its ability to trim the 3’end of yeast, S. cerevisiae, 5. 8S rRNA. In a historic account they laid out the events surrounding this identification and the subsequent birth of the research field. In the chapter by Kurt Januszyk and Christopher Lima the structural organization of eukaryotic exosomes and their evolutionary counterparts in bacteria and archaea are discussed in large part through presentation of structures.
Download or read book PCR Technology written by Henry Erlich and published by Springer. This book was released on 2015-12-31 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Book Synopsis Genetic Interactions Among Components of the Messenger RNA Biogenesis Machinery in the Yeast Saccharomyces Cerevisiae by : Ian Matthew Donaldson
Download or read book Genetic Interactions Among Components of the Messenger RNA Biogenesis Machinery in the Yeast Saccharomyces Cerevisiae written by Ian Matthew Donaldson and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overall goal of this thesis was to characterize mutations in the zinc-binding domain (ZBD) of the largest subunit (Rpo21p) of yeast RNA polymerase II and then use these mutations to find new genetic interactions with other components of the messenger RNA biogenesis machinery. The first data chapter demonstrates that the zinc stoichiometry of RNA polymerase II (RNAP II) is 7; consistent with the number of zinc-binding motifs present in the enzyme. Mutations in the Rpo21p-ZBD reduced RNAP II activity without visibly altering association of subunits required for this activity. The second data chapter describes the use of an Rpo21p-ZBD mutant in a synthetic-lethal screen designed to identify other components of the transcriptional machinery. Two mutant genes were identified; an allele of 'SRB5' (a known component of the RNAP II holoenzyme) and an allele of 'GCR3 ' (a factor that had previously been implicated in the expression of glycolytic genes). No evidence was found to suggest that Gcr3p is a component of the holoenzyme; its effect on 'in vitro' transcription appeared to be marginal and possibly indirect. Gcr3p was identified (by another group) as the largest subunit of the nuclear cap-binding complex and was shown to play a role in efficient splicing. The allele of 'GCR3' identified in this synthetic-lethal screen also had an effect on 'in vitro' splicing. I hypothesize that this defect, coupled with the transcriptional defect conferred by the zinc-binding domain mutation is sufficient to explain the synthetic-lethality observed between the two. The third data chapter describes an attempt to determine if Srb5p also plays a role in splicing. Extracts of the mutant 'SRB5' strain were deficient in splicing activity. This effect was likely an indirect one, possibly mediated by the effect of the 'SRB5' mutant allele on the gene expression of one or more splicing factors. Extracts made from Rpo21p-ZBD mutants also were reduced in splicing activity raising the possibility that splicing activity is sensitive to defects in components of the transcriptional machinery.
Book Synopsis The Role of Small Nucleolar Ribonucleoprotein Particles (snoRNPs) in Pre-rRNA Processing in Saccharomyces Cerevisiae by : John Patrick Morrissey
Download or read book The Role of Small Nucleolar Ribonucleoprotein Particles (snoRNPs) in Pre-rRNA Processing in Saccharomyces Cerevisiae written by John Patrick Morrissey and published by . This book was released on 1994 with total page 181 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles by : Max L. Birnstiel
Download or read book Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles written by Max L. Birnstiel and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade a most interesting story of the role played by the small nuclear RNPs, catalysts in RNA processing, has unfolded. Early investigations of the structure of these particles gave rise to hypotheses of their functions. As described in this book, these have been confirmed or are being tested by biochemical ex perimentation and most recently by the powerful technique of genetic analysis. Both biochemical and genetical approaches will also determine to what degree, and how, snRNPs and their cofactors participate in the differential expression of genes. As the details emerge and the number of snRNP species increases way beyond the six initially identified, one feels at the threshold of even greater things to come. The book covers many results in one of the most rapidly expanding fields of molecular biology. For this reason alone the specialist may detect some omissions and shortcomings. I hope they will be few. Instead of presenting a collation of conference reports with much overlap between them, this book has been written specially for the purpose of surveying the literature up to early 1987 in the snRNP field in a coherent manner, each of the seven chapters having been produced by connoisseurs of their field. While not each and every snRNP story can be covered in such a book, I hope that it will provide enjoyable and stimulating reading.
Book Synopsis Small Nucleolar RNAs (snoRNAs) From the Yeast Saccharomyces Cerevisiae by :
Download or read book Small Nucleolar RNAs (snoRNAs) From the Yeast Saccharomyces Cerevisiae written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Features a database of small nucleolar RNAs (snoRNAs) from the yeast Saccharomyces cerevisiae, compiled by Dmitry A. Samarsky and Maurille J. Fournier. Explains that the purposes of the database are to list the known snoRNAs from Saccharomyces cerevisiae, allow for comparison, disseminate information about each snoRNA, allow for manipulation of sequences, and provide references.
