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The Behavior Of Proteins At Solid Liquid Interfaces
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Book Synopsis The Behavior of Proteins at Solid-liquid Interfaces by : Adam Till Garland
Download or read book The Behavior of Proteins at Solid-liquid Interfaces written by Adam Till Garland and published by . This book was released on 2014 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: The behavior of a protein molecule at the solid-liquid interface is a worthy scientific problem for at least three reasons. The main driving force for studying this problem is a practical one, as many areas of bio-related technologies, such as medical implants, biosensing, and drug delivery, require the understanding of protein-surface interactions. In this dissertation, the nature of the precursive weakly adsorbed state of proteins during binding is reviewed. From this perspective, the adsorption and binding of proteins to a solid block copolymer thin film was achieved with regular spacing. Further efforts produced a monolayer of green fluorescent protein (GFP) covalently bound with regular spacing and orientation to a diblock copolymer thin film. This protein could be folded and refolded by changing solvent characteristics. We also explored the binding of DC-SIGN to mannose and mannotriose bearing lipid membranes. While no binding was observed, the usefulness of the lipid-based glycan microarray was proven using the well-studied CTB-GM1 binding motif.
Book Synopsis Proteins at Solid-Liquid Interfaces by : Philippe Déjardin
Download or read book Proteins at Solid-Liquid Interfaces written by Philippe Déjardin and published by Springer. This book was released on 2010-11-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.
Book Synopsis Proteins at Solid-Liquid Interfaces by : Philippe Déjardin
Download or read book Proteins at Solid-Liquid Interfaces written by Philippe Déjardin and published by Springer Science & Business Media. This book was released on 2006-09-28 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.
Book Synopsis Protein Adsorption at Solid-liquid Interfaces by : Gaurav Anand
Download or read book Protein Adsorption at Solid-liquid Interfaces written by Gaurav Anand and published by . This book was released on 2010 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Imaging of Protein Adsorption and Self-assembled Proteins at the Solid/liquid Interface by Atomic Force Microscopy by : David Tae Kim
Download or read book Imaging of Protein Adsorption and Self-assembled Proteins at the Solid/liquid Interface by Atomic Force Microscopy written by David Tae Kim and published by . This book was released on 2004 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteins at Interfaces II written by and published by . This book was released on 1995 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Investigation of protein behavior at gas-liquid interfaces by : Ivana Barackov
Download or read book Investigation of protein behavior at gas-liquid interfaces written by Ivana Barackov and published by . This book was released on 2013 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Instability at Interfaces During Drug Product Development by : Jinjiang Li
Download or read book Protein Instability at Interfaces During Drug Product Development written by Jinjiang Li and published by Springer Nature. This book was released on 2021-02-12 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are exposed to various interfacial stresses during drug product development. They are subjected to air-liquid, liquid-solid, and, sometimes, liquid-liquid interfaces throughout the development cycle-from manufacturing of drug substances to storage and drug delivery. Unlike small molecule drugs, proteins are typically unstable at interfaces where, on adsorption, they often denature and form aggregates, resulting in loss of efficacy and potential immunogenicity. This book covers both the fundamental aspects of proteins at interfaces and the quantification of interfacial behaviors of proteins. Importantly, this book introduces the industrial aspects of protein instabilities at interfaces, including the processes that introduce new interfaces, evaluation of interfacial instabilities, and mitigation strategies. The audience that this book targets encompasses scientists in the pharmaceutical and biotech industry, as well as faculty and students from academia in the surface science, pharmaceutical, and medicinal chemistry areas.
Book Synopsis Fluid Interfaces by : Eduardo Guzmán
Download or read book Fluid Interfaces written by Eduardo Guzmán and published by MDPI. This book was released on 2021-03-02 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluid interfaces are promising candidates for confining different types of materials, e.g., polymers, surfactants, colloids, and even small molecules, to be used in designing new functional materials with reduced dimensionality. The development of such materials requires a deepening of the physicochemical bases underlying the formation of layers at fluid interfaces as well as on the characterization of their structures and properties. This is of particular importance because the constraints associated with the assembly of materials at the interface lead to the emergence of equilibrium and features of dynamics in the interfacial systems, which are far removed from those conventionally found in traditional materials. This Special Issue is devoted to studies on the fundamental and applied aspects of fluid interfaces, and attempts to provide a comprehensive perspective on the current status of the research field.
Book Synopsis Proteins at Solid-liquid Interfaces by : Ananthakrishnan G. Sethuraman
Download or read book Proteins at Solid-liquid Interfaces written by Ananthakrishnan G. Sethuraman and published by . This book was released on 2004 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis On the Behaviour of Globular Proteins at the Solid/liquid Interface by : Johan C. Fröberg
Download or read book On the Behaviour of Globular Proteins at the Solid/liquid Interface written by Johan C. Fröberg and published by . This book was released on 1998 with total page 41 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Adsorption from Solution at the Solid/liquid Interface by : G. D. Parfitt
Download or read book Adsorption from Solution at the Solid/liquid Interface written by G. D. Parfitt and published by . This book was released on 1983 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Non-electrolytes. Adsorption of small molecules. Adsorption from mixtures of miscible liquids. Adsorption of nonionic surfactants. Adsorption of polymers. Electrolytes. Adsorption of small ions. Adsorption of ionic surfactants. Adsorption of dyes. Adsorption of polyelectrolytes from dilute solution.
Book Synopsis Protein Adsorption at the Solid/liquid Interface by : Richard James Marsh
Download or read book Protein Adsorption at the Solid/liquid Interface written by Richard James Marsh and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Bioseparations of Proteins by : Ajit Sadana
Download or read book Bioseparations of Proteins written by Ajit Sadana and published by Elsevier. This book was released on 1997-11-27 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation. Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations. Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures. Key Features * Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include: * detailed data on biological stability under various bioseparation conditions * extensive case studies from the literature on separation processes, validation, and economics * simplified analysis of protein refolding and inactivation mechanisms * consideration of adsorption theories and the effect of heterogeneity * coverage of both classical and novel bioseparation techniques, including chromatographic procedures
Book Synopsis Protein Self-Assembly by : Jennifer J. McManus
Download or read book Protein Self-Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Book Synopsis Physical Chemistry of Biological Interfaces by : Adam Baszkin
Download or read book Physical Chemistry of Biological Interfaces written by Adam Baszkin and published by CRC Press. This book was released on 1999-11-22 with total page 849 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introduction to the most important fundamental concepts of physicochemical interface science and a description of experimental techniques and applications of surface science in relation to biological systems. It explores artificial assemblies of lipids, proteins and polysaccharides that perform novel functions that living systems cannot duplicat
Book Synopsis Interfacial Phenomena and Bioproducts by : John Brash
Download or read book Interfacial Phenomena and Bioproducts written by John Brash and published by CRC Press. This book was released on 1996-08-16 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a synopsis of the theoretical principles and practical experience concerning the interfacial behaviour of bioproducts. The volume offers an interdisciplinary approach to the subject that highlights the importance of interfacial phenomena in bioprocessing systems, and the tools used to study and interpret the phenomena. It contains coverage ranging from fundamentals of bioproduct and solid surface structure to the interactions of multicomponent mixtures in heterogeneous.
