Techniques For The Analysis Of Membrane Proteins

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Techniques for the Analysis of Membrane Proteins

Author : C. Ragan
Publisher : Springer Science & Business Media
ISBN 13 : 9400940858
Total Pages : 447 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Techniques for the Analysis of Membrane Proteins by : C. Ragan

Download or read book Techniques for the Analysis of Membrane Proteins written by C. Ragan and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: A preface should justify the existence of the book it precedes and this is invariably done in scientific texts by reference to the explosive growth of the field since the last such volume appeared. In molecular biology, most fields can be justifiably described as growing explosively, as should be the case for a young and vigorous science, but the study of membrane proteins stands out as one which has taken giant strides in the last few years. Ignorance of the structure and function of membrane proteins at the molecular level was certainly not due to lack of interest but rather was a result of lack of appropriate techniques. It has above all been the development of new experimental methods which has wrenched membrane biochemistry out of what Anthony Martonosi fetchingly called its 'romantic phase' (Le. lots of ideas and few facts), into an era when the determination of membrane protein structure and mechanism is a reasonable goal. Membrane proteins are generally classified as peripheral or integral. Peripheral proteins are relatively easily dissociated from membranes by mild treatments whence their study is essentially no different to that of soluble proteins. This book therefore concentrates on integral proteins which are strongly bound to the membrane by hydrophobic interactions with lipids. A crucial step in their study is of necessity the d~velopment of methods of solubilization and purification under non-denaturing conditions.

Techniques for the Analysis of Membrane Proteins

Author : C Ragan
Publisher :
ISBN 13 : 9789400940864
Total Pages : 458 pages
Book Rating : 4.9/5 (48 download)

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Book Synopsis Techniques for the Analysis of Membrane Proteins by : C Ragan

Download or read book Techniques for the Analysis of Membrane Proteins written by C Ragan and published by . This book was released on 1986-10-16 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Biology of The Cell

Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of The Cell by : Bruce Alberts

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Membrane Protein Structure and Function Characterization

Author : Jean-Jacques Lacapere
Publisher : Humana Press
ISBN 13 : 9781493971497
Total Pages : 419 pages
Book Rating : 4.9/5 (714 download)

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Book Synopsis Membrane Protein Structure and Function Characterization by : Jean-Jacques Lacapere

Download or read book Membrane Protein Structure and Function Characterization written by Jean-Jacques Lacapere and published by Humana Press. This book was released on 2017-08-10 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Membrane Protein Protocols

Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
ISBN 13 : 159259400X
Total Pages : 330 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Membrane Protein Protocols by : Barry S. Selinsky

Download or read book Membrane Protein Protocols written by Barry S. Selinsky and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

The Next Generation in Membrane Protein Structure Determination

Author : Isabel Moraes
Publisher : Springer
ISBN 13 : 3319350722
Total Pages : 184 pages
Book Rating : 4.3/5 (193 download)

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Book Synopsis The Next Generation in Membrane Protein Structure Determination by : Isabel Moraes

Download or read book The Next Generation in Membrane Protein Structure Determination written by Isabel Moraes and published by Springer. This book was released on 2016-08-23 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins. This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes: Expression screening using fluorescent proteins The use of detergents in membrane protein research The use of NMR Synchrotron developments in membrane protein structural biology Visualisation and X-ray data collection of microcrystals X-ray diffraction data analysis from multiple crystals Serial millisecond crystallography Serial femtosecond crystallography Membrane protein structures in drug discovery The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work. Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.

Electron Paramagnetic Resonance: Volume 27

Author : Bela E. Bode
Publisher : Royal Society of Chemistry
ISBN 13 : 183916171X
Total Pages : 277 pages
Book Rating : 4.8/5 (391 download)

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Book Synopsis Electron Paramagnetic Resonance: Volume 27 by : Bela E. Bode

Download or read book Electron Paramagnetic Resonance: Volume 27 written by Bela E. Bode and published by Royal Society of Chemistry. This book was released on 2020-11-25 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron paramagnetic resonance (EPR) applications remain highly significant in modern analytical science and this volume compiles critical coverage of developments in the recent literature. The topics covered in this volume describe contrasting types of EPR application, including rapid scan EPR, using the EPR toolkit to investigate the structural dynamics of membrane proteins and pulse dipolar EPR spectroscopy for investigating biomolecular binding events. An additional chapter reviewing the PARACAT collaboration from the EU has also been included. Providing a snapshot of the area by a handpicked group of researchers at the cutting-edge of the field, this book is a useful addition to any library supporting this research.

Biophysics of Membrane Proteins

Author : Vincent L. G. Postis
Publisher : Humana
ISBN 13 : 9781071607237
Total Pages : 0 pages
Book Rating : 4.6/5 (72 download)

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Book Synopsis Biophysics of Membrane Proteins by : Vincent L. G. Postis

Download or read book Biophysics of Membrane Proteins written by Vincent L. G. Postis and published by Humana. This book was released on 2021-02-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers.

Membrane Protein Structure and Function Characterization

Author : Jean-Jacques Lacapere
Publisher : Humana
ISBN 13 : 9781493983995
Total Pages : 419 pages
Book Rating : 4.9/5 (839 download)

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Book Synopsis Membrane Protein Structure and Function Characterization by : Jean-Jacques Lacapere

Download or read book Membrane Protein Structure and Function Characterization written by Jean-Jacques Lacapere and published by Humana. This book was released on 2018-06-10 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Membrane Protein Structure Determination

Author : Jean-Jacques Lacapère
Publisher : Methods in Molecular Biology
ISBN 13 :
Total Pages : 482 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Membrane Protein Structure Determination by : Jean-Jacques Lacapère

Download or read book Membrane Protein Structure Determination written by Jean-Jacques Lacapère and published by Methods in Molecular Biology. This book was released on 2010-08-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.

Protein Analysis and Purification

Author : I.M. Rosenberg
Publisher : Springer Science & Business Media
ISBN 13 : 1475711085
Total Pages : 453 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Protein Analysis and Purification by : I.M. Rosenberg

Download or read book Protein Analysis and Purification written by I.M. Rosenberg and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip ment other than encouragement to read the manual, if you can find it. Through out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.

Structure and Function of Membrane Proteins

Author : Ingeborg Schmidt-Krey
Publisher : Humana
ISBN 13 : 9781071613931
Total Pages : 358 pages
Book Rating : 4.6/5 (139 download)

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Book Synopsis Structure and Function of Membrane Proteins by : Ingeborg Schmidt-Krey

Download or read book Structure and Function of Membrane Proteins written by Ingeborg Schmidt-Krey and published by Humana. This book was released on 2021-04-21 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles.

Biophysical Analysis of Membrane Proteins

Author : Eva Pebay-Peyroula
Publisher : John Wiley & Sons
ISBN 13 : 3527621237
Total Pages : 368 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Biophysical Analysis of Membrane Proteins by : Eva Pebay-Peyroula

Download or read book Biophysical Analysis of Membrane Proteins written by Eva Pebay-Peyroula and published by John Wiley & Sons. This book was released on 2008-06-25 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights. Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.

Membrane Proteomics

Author : Matthew J. Peirce
Publisher : Humana Press
ISBN 13 : 9781617378898
Total Pages : 0 pages
Book Rating : 4.3/5 (788 download)

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Book Synopsis Membrane Proteomics by : Matthew J. Peirce

Download or read book Membrane Proteomics written by Matthew J. Peirce and published by Humana Press. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The membranes surrounding cells and organelles constitute their interface with the local environment. The functions of membrane proteins include cell/cell and cell/extracellular matrix recognition, the reception and transduction of extracellular signals, and the tra- port of proteins, solutes and water molecules. Abnormal membrane protein expression has profound biological effects and may, for example, underlie phenotypic and functional differences between normal and tumour cells. Moreover the accessibility, particularly of plasma proteins traversing the plasma membrane of cells, makes them of particular ut- ity to the therapeutic intervention in disease. Indeed, it is estimated that of all currently licensed pharmaceuticals, approximately 70% target proteins resident in the plasma m- brane. In theory, unbiased technologies such as proteomics have the power to de?ne patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. However, although about 25% of open reading frames in fully sequenced genomes are estimated to encode integral membrane proteins, global analysis of membrane protein expression has proved problematic. Membrane protein analysis poses unique challenges at the level of extraction, solubilization, and separation in particular, and to a lesser extent of identi?cation and quantitation. These challenges have, however, fostered creativity, in- vation, and technical advances, many of which are brought together in Membrane P- teomics.

Heterologous Expression of Membrane Proteins

Author : Isabelle Mus-Veteau
Publisher : Springer Nature
ISBN 13 : 1071623680
Total Pages : 472 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Heterologous Expression of Membrane Proteins by : Isabelle Mus-Veteau

Download or read book Heterologous Expression of Membrane Proteins written by Isabelle Mus-Veteau and published by Springer Nature. This book was released on 2022-06-30 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.

Membrane Proteins Production for Structural Analysis

Author : Isabelle Mus-Veteau
Publisher : Springer
ISBN 13 : 1493906623
Total Pages : 441 pages
Book Rating : 4.4/5 (939 download)

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Book Synopsis Membrane Proteins Production for Structural Analysis by : Isabelle Mus-Veteau

Download or read book Membrane Proteins Production for Structural Analysis written by Isabelle Mus-Veteau and published by Springer. This book was released on 2014-06-20 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.

Membrane Protein Structure and Dynamics

Author : Nagarajan Vaidehi
Publisher : Humana Press
ISBN 13 : 9781493962334
Total Pages : 372 pages
Book Rating : 4.9/5 (623 download)

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Book Synopsis Membrane Protein Structure and Dynamics by : Nagarajan Vaidehi

Download or read book Membrane Protein Structure and Dynamics written by Nagarajan Vaidehi and published by Humana Press. This book was released on 2016-05-01 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Methods in Molecular Biology book details approaches to the structure, dynamics and interactions of membrane proteins, including measurement procedures and computational methods for structure prediction and insight on the dynamics of membrane receptors."