Sumo: Methods and Protocols

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Publisher : Methods in Molecular Biology
ISBN 13 : 9781493981755
Total Pages : 322 pages
Book Rating : 4.9/5 (817 download)

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Book Synopsis Sumo: Methods and Protocols by : Manuel S. Rodriguez

Download or read book Sumo: Methods and Protocols written by Manuel S. Rodriguez and published by Methods in Molecular Biology. This book was released on 2018-09-22 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt:

SUMO Protocols

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Author :
Publisher :
ISBN 13 : 9781597455664
Total Pages : 324 pages
Book Rating : 4.4/5 (556 download)

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Book Synopsis SUMO Protocols by : Helle D. Ulrich

Download or read book SUMO Protocols written by Helle D. Ulrich and published by . This book was released on 2009 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: The functions of the ubiquitin-related modifier SUMO are highly diverse and largely independent of the ubiquitin system. In this volume, leading experts present methods that highlight SUMO 's unique biological features in order to support future SUMO research.

SUMO Protocols

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Author :
Publisher : Humana Press
ISBN 13 : 9781934115800
Total Pages : 0 pages
Book Rating : 4.1/5 (158 download)

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Book Synopsis SUMO Protocols by : Helle Ulrich

Download or read book SUMO Protocols written by Helle Ulrich and published by Humana Press. This book was released on 2008-10-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Post-translational protein modifications by members of the ubiquitin family are widely recognized as important regulatory control systems for a variety of biological pathways. Their influence on eukaryotic cellular metabolism is comparable to that of other modifi- tions such as phosphorylation, acetylation and methylation. The small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself; yet, the functions of the SUMO system are highly diverse and largely independent of the ubiquitin system. SUMO modification controls the activity of tr- scription factors and can influence protein stability, but it also contributes to nucleo-cy- plasmic transport, chromosome segregation and DNA damage repair. As a consequence, the SUMO system pervades virtually all areas of basic molecular and cell biology, and scientists from different backgrounds, including medical researchers, are likely to enco- ter SUMO in the course of their studies. This volume, SUMO Protocols, therefore aims at presenting a collection of methods relevant to SUMO research in order to make these tools available to biochemists, molecular and cell biologists as well as research-oriented clinicians not yet familiar with the system. In contrast to the ubiquitin system, which has been the subject of several reviews and methods collections, no practical compendium entirely devoted to SUMO has been p- lished.

SUMO Regulation of Cellular Processes

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Author :
Publisher : Springer
ISBN 13 : 3319500449
Total Pages : 413 pages
Book Rating : 4.3/5 (195 download)

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Book Synopsis SUMO Regulation of Cellular Processes by : Van G. Wilson

Download or read book SUMO Regulation of Cellular Processes written by Van G. Wilson and published by Springer. This book was released on 2017-02-13 with total page 413 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second edition of a very well received book that details how the sumoylation system functions and how it modulates numerous cellular activities. SUMO is a post-translational modifier in the ubiquitin super-family that has gained recognition over the last twenty years as an essential and prevalent regulatory molecule. Individual chapters explore the biochemistry, molecular biology, and cell biology of the sumoylation system and its substrate proteins. The book is divided into three themed parts: Molecular Functions (I), Cell Growth Regulation (II), and Diseases (III). Parts I and II focus on the contribution of sumoylation to cellular activities in both the nuclear and cytoplasmic compartments. The nuclear activities covered include nucleic acid metabolism (both RNA and DNA), chromosome structure and replication, and nucleocytoplasmic transport. Cytoplasmic processes presented include regulation of membrane ion channels, general metabolism, and apoptotic signalling. Topics in Part III include the role of sumoylation in developmental abnormalities (craniofacial and cardiovascular), diabetes, neurodegenerative diseases, cancer, and infections with viruses and bacteria. Each of the corresponding chapter authors is an active researcher who has made significant contributions to understanding sumoylation. This second edition provides updates and revisions to most of the original chapters plus adds six new chapters to address important developing areas of sumoylation research. This volume is intended for a scientific audience from undergraduates to independent researchers. The content will serve as both a solid introduction for the novice reader and an in depth treatment for the advanced scholar.

Plant Proteostasis

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Publisher : Springer Nature
ISBN 13 : 1071627848
Total Pages : 408 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Plant Proteostasis by : L. Maria Lois

Download or read book Plant Proteostasis written by L. Maria Lois and published by Springer Nature. This book was released on 2022-11-22 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field.

Ubiquitin-Proteasome Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592598951
Total Pages : 376 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Ubiquitin-Proteasome Protocols by : Cam Patterson

Download or read book Ubiquitin-Proteasome Protocols written by Cam Patterson and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome-dependent functions. State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes.

SILAC

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Publisher : Springer Nature
ISBN 13 : 1071628631
Total Pages : 287 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis SILAC by : Jose L. Luque-Garcia

Download or read book SILAC written by Jose L. Luque-Garcia and published by Springer Nature. This book was released on 2022-11-12 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.

Protein Chromatography

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Publisher : Springer Nature
ISBN 13 : 1071633627
Total Pages : 495 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Protein Chromatography by : Sinéad T Loughran

Download or read book Protein Chromatography written by Sinéad T Loughran and published by Springer Nature. This book was released on 2023-10-01 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.

Sumo Skills

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Publisher :
ISBN 13 : 9780991408603
Total Pages : 162 pages
Book Rating : 4.4/5 (86 download)

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Book Synopsis Sumo Skills by : Thomas Zabel

Download or read book Sumo Skills written by Thomas Zabel and published by . This book was released on 2014-05-13 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first English-language training guide for Sumo wrestling! The Japanese cornered the market in producing the best athletes in the sport of amateur sumo until an American, Emmanuel Yarborough, won the GOLD in the open-weight division at the 4th World Sumo Championships in 1995. Since then, sumo is one of the fastest growing sports in the United States, Europe, Asia, South America, and around the world. A sumo match can be over in a fraction of a second. It's amazingly explosive! The burst of energy and adrenaline makes sumo as physically and mentally intense as any sport can be. This instructional handbook gives you the basic fundamental skills to train and succeed in competitive amateur sumo. It begins with a short history, covers how to tie a mawashi, and goes on to detail the commands and responsibilities of the referee (gyoji) and judges (shinpan). This ultimate manual gives step-by-step examples of the most common techniques used by both amateur and professional sumo rikishi, and breaks down various training methods such as shiko, suriashi, butsukari, etc. so that anyone, with enough motivation, training, and preparation, can become proficient at sumo.

Methods in Animal Proteomics

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Publisher : John Wiley & Sons
ISBN 13 : 0813817919
Total Pages : 464 pages
Book Rating : 4.8/5 (138 download)

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Book Synopsis Methods in Animal Proteomics by : Philip Whitfield

Download or read book Methods in Animal Proteomics written by Philip Whitfield and published by John Wiley & Sons. This book was released on 2011-09-13 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.

SUMO Protocols

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Author :
Publisher : Humana Press
ISBN 13 : 9781934115800
Total Pages : 324 pages
Book Rating : 4.1/5 (158 download)

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Book Synopsis SUMO Protocols by : Helle Ulrich

Download or read book SUMO Protocols written by Helle Ulrich and published by Humana Press. This book was released on 2008-10-23 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Post-translational protein modifications by members of the ubiquitin family are widely recognized as important regulatory control systems for a variety of biological pathways. Their influence on eukaryotic cellular metabolism is comparable to that of other modifi- tions such as phosphorylation, acetylation and methylation. The small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself; yet, the functions of the SUMO system are highly diverse and largely independent of the ubiquitin system. SUMO modification controls the activity of tr- scription factors and can influence protein stability, but it also contributes to nucleo-cy- plasmic transport, chromosome segregation and DNA damage repair. As a consequence, the SUMO system pervades virtually all areas of basic molecular and cell biology, and scientists from different backgrounds, including medical researchers, are likely to enco- ter SUMO in the course of their studies. This volume, SUMO Protocols, therefore aims at presenting a collection of methods relevant to SUMO research in order to make these tools available to biochemists, molecular and cell biologists as well as research-oriented clinicians not yet familiar with the system. In contrast to the ubiquitin system, which has been the subject of several reviews and methods collections, no practical compendium entirely devoted to SUMO has been p- lished.

Pattern Recognition in Bioinformatics

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 3540884343
Total Pages : 488 pages
Book Rating : 4.5/5 (48 download)

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Book Synopsis Pattern Recognition in Bioinformatics by : Madhu Chetty

Download or read book Pattern Recognition in Bioinformatics written by Madhu Chetty and published by Springer Science & Business Media. This book was released on 2008-09-29 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the post-genomic era, a holistic understanding of biological systems and p- cesses,inalltheircomplexity,is criticalincomprehendingnature’schoreography of life. As a result, bioinformatics involving its two main disciplines, namely, the life sciences and the computational sciences, is fast becoming a very promising multidisciplinary research ?eld. With the ever-increasing application of lar- scalehigh-throughputtechnologies,suchasgeneorproteinmicroarraysandmass spectrometry methods, the enormous body of information is growing rapidly. Bioinformaticians are posed with a large number of di?cult problems to solve, arising not only due to the complexities in acquiring the molecular infor- tion but also due to the size and nature of the generated data sets and/or the limitations of the algorithms required for analyzing these data. Although the ?eld of bioinformatics is still in its embryonic stage, the recent advancements in computational and information-theoretic techniques are enabling us to c- ductvariousinsilicotestingandscreeningofmanylab-basedexperimentsbefore these are actually performed in vitro or in vivo. These in silico investigations are providing new insights for interpretation and establishing a new direction for a deeper understanding. Among the various advanced computational methods currently being applied to such studies, the pattern recognition techniques are mostly found to be at the core of the whole discovery process for apprehending the underlying biological knowledge. Thus, we can safely surmise that the - going bioinformatics revolution may, in future, inevitably play a major role in many aspects of medical practice and/or the discipline of life sciences.

Formal Methods: Foundations and Applications

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Publisher : Springer Nature
ISBN 13 : 3030921379
Total Pages : 149 pages
Book Rating : 4.0/5 (39 download)

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Book Synopsis Formal Methods: Foundations and Applications by : Sérgio Campos

Download or read book Formal Methods: Foundations and Applications written by Sérgio Campos and published by Springer Nature. This book was released on 2021-11-25 with total page 149 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book constitutes the refereed proceedings of the 24rd Brazilian Symposium on Formal Methods, SBMF 2021, which was held in December 2021. Due to COVID 19-pandemic it took place virtually. The 8 regular papers presented in this book were carefully reviewed and selected from 15 submissions. The papers detail the development, dissemination, and use of formal methods for the construction of high-quality computational systems, aiming to promote opportunities for researchers and practitioners with an interest in formal methods to discuss the recent advances in this area

Protein Affinity Tags

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Publisher : Humana
ISBN 13 : 9781493910335
Total Pages : 213 pages
Book Rating : 4.9/5 (13 download)

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Book Synopsis Protein Affinity Tags by : Richard J. Giannone

Download or read book Protein Affinity Tags written by Richard J. Giannone and published by Humana. This book was released on 2014-06-19 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein’s function and structure. Today’s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to “fish out” a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information – information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of today’s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest.

Advances in Plant Meiosis: From Model Species to Crops

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Author :
Publisher : Frontiers Media SA
ISBN 13 : 2889634280
Total Pages : 267 pages
Book Rating : 4.8/5 (896 download)

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Book Synopsis Advances in Plant Meiosis: From Model Species to Crops by : Tomás Naranjo

Download or read book Advances in Plant Meiosis: From Model Species to Crops written by Tomás Naranjo and published by Frontiers Media SA. This book was released on 2020-01-28 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein Affinity Tags

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Author :
Publisher : Humana Press
ISBN 13 : 9781493910359
Total Pages : 213 pages
Book Rating : 4.9/5 (13 download)

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Book Synopsis Protein Affinity Tags by : Richard J. Giannone

Download or read book Protein Affinity Tags written by Richard J. Giannone and published by Humana Press. This book was released on 2014-06-20 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein’s function and structure. Today’s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to “fish out” a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information – information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of today’s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest.

Single Molecule Analysis

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Author :
Publisher : Springer Nature
ISBN 13 : 1071633775
Total Pages : 511 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Single Molecule Analysis by : Iddo Heller

Download or read book Single Molecule Analysis written by Iddo Heller and published by Springer Nature. This book was released on 2023-11-13 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.