Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Download Structural Studies Of Membrane Proteins Using Transmission Electron Microscopy full books in PDF, epub, and Kindle. Read online Structural Studies Of Membrane Proteins Using Transmission Electron Microscopy ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author :
Publisher :
ISBN 13 : 9789175954684
Total Pages : 55 pages
Book Rating : 4.9/5 (546 download)
Download or read book Structural Studies of Membrane Proteins Using Transmission Electron Microscopy written by and published by . This book was released on 2015 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Joel Reuben Meyerson
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (16 download)
Download or read book Structural Studies of Membrane Proteins and Cellular Architecture Using Three-dimensional Electron Microscopy written by Joel Reuben Meyerson and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Breanna Sachiko Vollmar
Publisher :
ISBN 13 :
Total Pages : 252 pages
Book Rating : 4.:/5 (879 download)
Download or read book Investigating the Structure of Macromolecular Complexes and Membrane Proteins Using Transmission Electron Microscopy written by Breanna Sachiko Vollmar and published by . This book was released on 2013 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transmission electron microscopy (TEM) has provided atomic resolution details regarding the biological processes that give rise to life. The work presented here focuses on the application of TEM for studying membrane proteins and macromolecular assemblies using electron crystallography and single particle electron cryomicroscopy (cryo-EM). Two membrane proteins, human aquaporin 3 (hAQP3) and galactose permease (GalP) from Escherichia coli, were studied using electron crystallography. hAQP3 was successfully expressed in Pichia pastoris and purified, but failed to reconstitute to form two-dimensional crystals. Two-dimensional crystals of GalP were optimized to form large, crystalline vesicles and sheets. A projection map of GalP calculated from a cryo-EM image showed that GalP forms trimers in the two-dimensional crystals. Single particle TEM studies focused on three macromolecular assemblies, alphaB-crystallin, a designed octahedral protein cage and a mutant HCV IRES-40S ribosome complex. Projection class averages of negatively stained alphaB-crystallin were combined with NMR and SAXS data to produce a pseudo-atomic model of alphaB-crystallin and provide a hypothesis for multimerization. A single particle cryo-EM reconstruction of a designed self-assembling octahedral protein oligomer showed that a designed protein assembly forms the predicted symmetry in solution. A 17 [angstrom] resolution single particle cryo-EM reconstruction of a mutant HCV IRES bound to the 40S ribosomal subunit revealed that deletion of three nucleotides in the IRES modulate interaction with the 40S ribosomal subunit. The conformational changes induced in the 40S were shown to affect a step after 80S formation, the transition from initiation to elongation. These studies illustrate the application of TEM to study the molecular mechanisms used by both membrane proteins and macromolecular complexes to carry out biological processes.
Author : C. Ragan
Publisher : Springer Science & Business Media
ISBN 13 : 9400940858
Total Pages : 447 pages
Book Rating : 4.4/5 (9 download)
Download or read book Techniques for the Analysis of Membrane Proteins written by C. Ragan and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: A preface should justify the existence of the book it precedes and this is invariably done in scientific texts by reference to the explosive growth of the field since the last such volume appeared. In molecular biology, most fields can be justifiably described as growing explosively, as should be the case for a young and vigorous science, but the study of membrane proteins stands out as one which has taken giant strides in the last few years. Ignorance of the structure and function of membrane proteins at the molecular level was certainly not due to lack of interest but rather was a result of lack of appropriate techniques. It has above all been the development of new experimental methods which has wrenched membrane biochemistry out of what Anthony Martonosi fetchingly called its 'romantic phase' (Le. lots of ideas and few facts), into an era when the determination of membrane protein structure and mechanism is a reasonable goal. Membrane proteins are generally classified as peripheral or integral. Peripheral proteins are relatively easily dissociated from membranes by mild treatments whence their study is essentially no different to that of soluble proteins. This book therefore concentrates on integral proteins which are strongly bound to the membrane by hydrophobic interactions with lipids. A crucial step in their study is of necessity the d~velopment of methods of solubilization and purification under non-denaturing conditions.
Author : Jean-Jacques Lacapère
Publisher : Humana Press
ISBN 13 : 9781607617631
Total Pages : 459 pages
Book Rating : 4.6/5 (176 download)
Download or read book Membrane Protein Structure Determination written by Jean-Jacques Lacapère and published by Humana Press. This book was released on 2011-08-24 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.
Author : Jean-Luc Popot
Publisher : Springer
ISBN 13 : 3319731483
Total Pages : 724 pages
Book Rating : 4.3/5 (197 download)
Download or read book Membrane Proteins in Aqueous Solutions written by Jean-Luc Popot and published by Springer. This book was released on 2018-06-08 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first to be entirely devoted to the challenging art of handling membrane proteins out of their natural environment, a key process in biological and pharmaceutical research, but one plagued with difficulties and pitfalls. Written by one of the foremost experts in the field, Membrane Proteins in Aqueous Solutions is accessible to any member of a membrane biology laboratory. After presenting the structure, functions, dynamics, synthesis, natural environment and lipid interactions of membrane proteins, the author discusses the principles of extracting them with detergents, the mechanisms of detergent-induced destabilization, countermeasures, and recent progress in developing detergents with weaker denaturing properties. Non-conventional alternatives to detergents, including bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants and amphipols, are described, and their relative advantages and drawbacks are compared. The synthesis and solution properties of the various types of amphipols are presented, as well as the formation and properties of membrane protein/amphipol complexes and the transfer of amphipol-trapped proteins to detergents, nanodiscs, lipidic mesophases, or living cells. The final chapters of the book deal with applications: membrane protein in vitro folding and cell-free expression, solution studies, NMR, crystallography, electron microscopy, mass spectrometry, amphipol-mediated immobilization of membrane proteins, and biomedical applications. Important features of the book include introductory sections describing foundations as well as the state-of-the-art for each of the biophysical techniques discussed, and topical tables which organize a widely dispersed literature. Boxes and annexes throughout the book explain technical aspects, and twelve detailed experimental protocols, ranging from in vitro folding of membrane proteins to single-particle electron cryomicroscopy, have been contributed by and commented on by experienced users. Membrane Proteins in Aqueous Solutions offers a concise, accessible introduction to membrane protein biochemistry and biophysics, as well as comprehensive coverage of the properties and uses of conventional and non-conventional surfactants. It will be useful both in basic and applied research laboratories and as a teaching aid for students, instructors, researchers, and professionals within the field.
Author : Reinhard Grisshammer
Publisher : Royal Society of Chemistry
ISBN 13 : 1847552560
Total Pages : 417 pages
Book Rating : 4.8/5 (475 download)
Download or read book Structural Biology of Membrane Proteins written by Reinhard Grisshammer and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last few years there have been many exciting and innovative developments in the field of membrane protein structure and this trend is set to continue. Structural Biology of Membrane Proteins is a new monograph covering a wide range of topics with contributions from leading experts in the field. The book is split into three sections: the first discusses topics such as expression, purification and crystallisation; the second covers characterisation techniques and the final section looks at new protein structures. The book will hence have wide appeal to researchers working in and around the field and provide an up-to-date reference source. Introductory sections to each topic are accompanied by more detailed discussions for the more experienced biochemist. Detailed descriptions of experimental methods are included to demonstrate practical approaches to membrane protein structure projects. The book also offers an up-to-date reference source in addition to descriptions of new and emerging developments, including state-of-the-art techniques for solving membrane protein structures. Structural Biology of Membrane Proteins encompasses both basic introductions and detailed descriptions of themes and should appeal to a wide range of biochemical scientists, both experienced and beginner.
Author : Thomas Walz
Publisher :
ISBN 13 :
Total Pages : 132 pages
Book Rating : 4.:/5 (8 download)
Download or read book Structural Analysis of Membrane Proteins by Electron Microscopy written by Thomas Walz and published by . This book was released on 1996 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Katarzyna Beata Mościcka
Publisher :
ISBN 13 : 9789036737432
Total Pages : 148 pages
Book Rating : 4.7/5 (374 download)
Download or read book Structural Investigation of Membrane Proteins by Electron Microscopy written by Katarzyna Beata Mościcka and published by . This book was released on 2009 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sepehr Eskandari
Publisher :
ISBN 13 :
Total Pages : 446 pages
Book Rating : 4.:/5 ( download)
Download or read book Structural Analysis of Integral Membrane Proteins by Freeze-fracture Electron Microscopy written by Sepehr Eskandari and published by . This book was released on 1999 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jean-Jacques Lacapère
Publisher : Methods in Molecular Biology
ISBN 13 :
Total Pages : 482 pages
Book Rating : 4.F/5 ( download)
Download or read book Membrane Protein Structure Determination written by Jean-Jacques Lacapère and published by Methods in Molecular Biology. This book was released on 2010-08-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.
Author : Ingeborg Schmidt-Krey
Publisher : Humana
ISBN 13 : 9781071613931
Total Pages : 358 pages
Book Rating : 4.6/5 (139 download)
Download or read book Structure and Function of Membrane Proteins written by Ingeborg Schmidt-Krey and published by Humana. This book was released on 2021-04-21 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles.
Author : Elizabeth Morrison Wilson-Kubalek
Publisher :
ISBN 13 : 9780542002434
Total Pages : 344 pages
Book Rating : 4.0/5 (24 download)
Download or read book Protein Structure Determination on Lipid Substrates by Electron Microscopy written by Elizabeth Morrison Wilson-Kubalek and published by . This book was released on 2005 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the impressive growth in gene sequence data and advances in genetics the number of proteins and macromolecular assemblies for structural studies are increasing at a rapid rate. Structural biology will help to unravel the function of the newly discovered proteins and their molecular interactions within the cell and will ultimately provide the framework for developing new therapeutic drugs. Electron crystallography has become a valuable method for the structure determination of proteins and macromolecular complexes that are large or heterogeneous, difficult to obtain, unstable, insoluble, or that fail to yield crystals suitable for X-ray crystallographic analysis. Although electron microscopy (EM) and image analysis methods are rapidly improving and continuing to prove their value, advances in sample preparation have been limited, and general methods for obtaining ordered arrays of proteins for EM analysis are needed. In this thesis, I have described a method for promoting the formation of helical protein arrays. Helical crystallization on lipid nanotubes presents an alternative platform to explore fundamentals of protein ordering, intermolecular protein interaction and phase behavior. Three-dimensional (3-D) maps of wild-type and mutant streptavidin (SA) show that the molecular packing arrangements of SA on the surface of the lipid nanotubes differ from previously reported lattice packing of SA on biotinylated monolayers. His-tagged Perfringolysin O (PFO), a soluble toxin was used to demonstrate the utility of nickel-lipid nanotubes as a tool for structural studies of His-tagged proteins. Cryo-EM and image analysis of PFO helical arrays were used to reconstruct a 3-D density map. These studies have demonstrated that lipid nanotubes offer a robust and reproducible substrate for forming helical protein arrays which present a means for studying protein structure and structure-function relationships. Using EM and single-particle image analysis I calculated 2-D projection maps from images of oligomeric PFO prepore and pore complexes formed on lipid layers and identified an apparent conformational change from the prepore to the pore. In addition, I identified specific domains of the toxin molecule within the image and have thus determined the orientation of PFO monomers within the oligomeric complex.
Author : Jean-Paul Renaud
Publisher : John Wiley & Sons
ISBN 13 : 1118900502
Total Pages : 1367 pages
Book Rating : 4.1/5 (189 download)
Download or read book Structural Biology in Drug Discovery written by Jean-Paul Renaud and published by John Wiley & Sons. This book was released on 2020-01-09 with total page 1367 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the most comprehensive and up-to-date overview of structure-based drug discovery covering both experimental and computational approaches, Structural Biology in Drug Discovery: Methods, Techniques, and Practices describes principles, methods, applications, and emerging paradigms of structural biology as a tool for more efficient drug development. Coverage includes successful examples, academic and industry insights, novel concepts, and advances in a rapidly evolving field. The combined chapters, by authors writing from the frontlines of structural biology and drug discovery, give readers a valuable reference and resource that: Presents the benefits, limitations, and potentiality of major techniques in the field such as X-ray crystallography, NMR, neutron crystallography, cryo-EM, mass spectrometry and other biophysical techniques, and computational structural biology Includes detailed chapters on druggability, allostery, complementary use of thermodynamic and kinetic information, and powerful approaches such as structural chemogenomics and fragment-based drug design Emphasizes the need for the in-depth biophysical characterization of protein targets as well as of therapeutic proteins, and for a thorough quality assessment of experimental structures Illustrates advances in the field of established therapeutic targets like kinases, serine proteinases, GPCRs, and epigenetic proteins, and of more challenging ones like protein-protein interactions and intrinsically disordered proteins
Author : Frank Joachim
Publisher : Elsevier
ISBN 13 : 0080525814
Total Pages : 361 pages
Book Rating : 4.0/5 (85 download)
Download or read book Three-Dimensional Electron Microscopy of Macromolecular Assemblies written by Frank Joachim and published by Elsevier. This book was released on 1996-01-24 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized* Describes methods that have been instrumental in exploring the three-dimensional structure of* the nuclear pore complex* the calcium release channel;* the ribosome* chaperonins
Author : George Henry Addona
Publisher :
ISBN 13 :
Total Pages : 364 pages
Book Rating : 4.:/5 (418 download)
Download or read book Structural Studies of Membrane Proteins by Electron Paramagnetic Resonance Spectroscopy and Mass Spectrometry written by George Henry Addona and published by . This book was released on 1995 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
