Structural Characterization Of Macromolecular Complexes Within Thick Specimens Using Cryo Focused Ion Beam Scanning Electron Microscopy Cryo Fib Sem And Cryo Electron Tomography Cryo Et

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Correlative Imaging

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Author : Paul Verkade
Publisher : John Wiley & Sons
ISBN 13 : 1119086450
Total Pages : 245 pages
Book Rating : 4.1/5 (19 download)

Book Synopsis Correlative Imaging by : Paul Verkade

Download or read book Correlative Imaging written by Paul Verkade and published by John Wiley & Sons. This book was released on 2019-11-04 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Brings a fresh point of view to the current state of correlative imaging and the future of the field This book provides contributions from international experts on correlative imaging, describing their vision of future developments in the field based on where it is today. Starting with a brief historical overview of how the field evolved, it presents the latest developments in microscopy that facilitate the correlative workflow. It also discusses the need for an ideal correlative probe, applications in proteomic and elemental analysis, interpretation methods, and how correlative imaging can incorporate force microscopy, soft x-ray tomography, and volume electron microscopy techniques. Work on placing individual molecules within cells is also featured. Correlative Imaging: Focusing on the Future offers in-depth chapters on: correlative imaging from an LM perspective; the importance of sample processing for correlative imaging; correlative light and volume EM; correlation with scanning probe microscopies; and integrated microscopy. It looks at: cryo-correlative microscopy; correlative cryo soft X-ray imaging; and array tomography. Hydrated-state correlative imaging in vacuo, correlating data from different imaging modalities, and big data in correlative imaging are also considered. Brings a fresh view to one of the hottest topics within the imaging community: the correlative imaging field Discusses current research and offers expert thoughts on the field’s future developments Presented by internationally-recognized editors and contributors with extensive experience in research and applications Of interest to scientists working in the fields of imaging, structural biology, cell biology, developmental biology, neurobiology, cancer biology, infection and immunity, biomaterials and biomedicine Part of the Wiley–Royal Microscopical Society series Correlative Imaging: Focusing on the Future will appeal to those working in the expanding field of the biosciences, correlative microscopy and related microscopic areas. It will also benefit graduate students working in microscopy, as well as anyone working in the microscopy imaging field in biomedical research.

Liquid Cell Electron Microscopy

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Author : Frances M. Ross
Publisher : Cambridge University Press
ISBN 13 : 1107116570
Total Pages : 529 pages
Book Rating : 4.1/5 (71 download)

Book Synopsis Liquid Cell Electron Microscopy by : Frances M. Ross

Download or read book Liquid Cell Electron Microscopy written by Frances M. Ross and published by Cambridge University Press. This book was released on 2017 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: 2.6.2 Electrodes for Electrochemistry

Scanning Microscopy for Nanotechnology

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Author : Weilie Zhou
Publisher : Springer Science & Business Media
ISBN 13 : 0387396209
Total Pages : 533 pages
Book Rating : 4.3/5 (873 download)

Book Synopsis Scanning Microscopy for Nanotechnology by : Weilie Zhou

Download or read book Scanning Microscopy for Nanotechnology written by Weilie Zhou and published by Springer Science & Business Media. This book was released on 2007-03-09 with total page 533 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents scanning electron microscopy (SEM) fundamentals and applications for nanotechnology. It includes integrated fabrication techniques using the SEM, such as e-beam and FIB, and it covers in-situ nanomanipulation of materials. The book is written by international experts from the top nano-research groups that specialize in nanomaterials characterization. The book will appeal to nanomaterials researchers, and to SEM development specialists.

Atom Probe Tomography

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Author : Michael K. Miller
Publisher : Springer Science & Business Media
ISBN 13 : 1461542812
Total Pages : 247 pages
Book Rating : 4.4/5 (615 download)

Book Synopsis Atom Probe Tomography by : Michael K. Miller

Download or read book Atom Probe Tomography written by Michael K. Miller and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: The microanalytical technique of atom probe tomography (APT) permits the spatial coordinates and elemental identities of the individual atoms within a small volume to be determined with near atomic resolution. Therefore, atom probe tomography provides a technique for acquiring atomic resolution three dimensional images of the solute distribution within the microstructures of materials. This monograph is designed to provide researchers and students the necessary information to plan and experimentally conduct an atom probe tomography experiment. The techniques required to visualize and to analyze the resulting three-dimensional data are also described. The monograph is organized into chapters each covering a specific aspect of the technique. The development of this powerful microanalytical technique from the origins offield ion microscopy in 1951, through the first three-dimensional atom probe prototype built in 1986 to today's commercial state-of-the-art three dimensional atom probe is documented in chapter 1. A general introduction to atom probe tomography is also presented in chapter 1. The various methods to fabricate suitable needle-shaped specimens are presented in chapter 2. The procedure to form field ion images of the needle-shaped specimen is described in chapter 3. In addition, the appearance of microstructural features and the information that may be estimated from field ion microscopy are summarized. A brief account of the theoretical basis for processes of field ionization and field evaporation is also included.

Electron Microscopy of Plant Cells

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Author : C Hawes
Publisher : Academic Press
ISBN 13 : 0323148980
Total Pages : 477 pages
Book Rating : 4.3/5 (231 download)

Book Synopsis Electron Microscopy of Plant Cells by : C Hawes

Download or read book Electron Microscopy of Plant Cells written by C Hawes and published by Academic Press. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

Introduction to Focused Ion Beams

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Author : Lucille A. Giannuzzi
Publisher : Springer Science & Business Media
ISBN 13 : 038723313X
Total Pages : 362 pages
Book Rating : 4.3/5 (872 download)

Book Synopsis Introduction to Focused Ion Beams by : Lucille A. Giannuzzi

Download or read book Introduction to Focused Ion Beams written by Lucille A. Giannuzzi and published by Springer Science & Business Media. This book was released on 2006-05-18 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to Focused Ion Beams is geared towards techniques and applications. This is the only text that discusses and presents the theory directly related to applications and the only one that discusses the vast applications and techniques used in FIBs and dual platform instruments.

Proteomics Data Analysis

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Author : Daniela Cecconi
Publisher :
ISBN 13 : 9781071616413
Total Pages : 326 pages
Book Rating : 4.6/5 (164 download)

Book Synopsis Proteomics Data Analysis by : Daniela Cecconi

Download or read book Proteomics Data Analysis written by Daniela Cecconi and published by . This book was released on 2021 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thorough book collects methods and strategies to analyze proteomics data. It is intended to describe how data obtained by gel-based or gel-free proteomics approaches can be inspected, organized, and interpreted to extrapolate biological information. Organized into four sections, the volume explores strategies to analyze proteomics data obtained by gel-based approaches, different data analysis approaches for gel-free proteomics experiments, bioinformatic tools for the interpretation of proteomics data to obtain biological significant information, as well as methods to integrate proteomics data with other omics datasets including genomics, transcriptomics, metabolomics, and other types of data. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that will ensure high quality results in the lab. Authoritative and practical, Proteomics Data Analysis serves as an ideal guide to introduce researchers, both experienced and novice, to new tools and approaches for data analysis to encourage the further study of proteomics.

Freezing Colloids: Observations, Principles, Control, and Use

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Author : Sylvain Deville
Publisher : Springer
ISBN 13 : 3319505157
Total Pages : 618 pages
Book Rating : 4.3/5 (195 download)

Book Synopsis Freezing Colloids: Observations, Principles, Control, and Use by : Sylvain Deville

Download or read book Freezing Colloids: Observations, Principles, Control, and Use written by Sylvain Deville and published by Springer. This book was released on 2017-01-19 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive overview of the freezing of colloidal suspensions and explores cutting-edge research in the field. It is the first book to deal with this phenomenon from a multidisciplinary perspective, and examines the various occurrences, their technological uses, the fundamental phenomena, and the different modeling approaches. Its chapters integrate input from fields as diverse as materials science, physics, biology, mathematics, geophysics, and food science, and therefore provide an excellent point of departure for anyone interested in the topic. The main content is supplemented by a wealth of figures and illustrations to elucidate the concepts presented, and includes a final chapter providing advice for those starting out in the field. As such, the book provides an invaluable resource for materials scientists, physicists, biologists, and mathematicians, and will also benefit food engineers, civil engineers, and materials processing professionals.

Cryotechniques in Biological Electron Microscopy

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Author : Rudolf A. Steinbrecht
Publisher : Springer Science & Business Media
ISBN 13 : 3642728154
Total Pages : 308 pages
Book Rating : 4.6/5 (427 download)

Book Synopsis Cryotechniques in Biological Electron Microscopy by : Rudolf A. Steinbrecht

Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Characterization of Minerals, Metals, and Materials 2019

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Author : Bowen Li
Publisher : Springer
ISBN 13 : 3030057496
Total Pages : 782 pages
Book Rating : 4.0/5 (3 download)

Book Synopsis Characterization of Minerals, Metals, and Materials 2019 by : Bowen Li

Download or read book Characterization of Minerals, Metals, and Materials 2019 written by Bowen Li and published by Springer. This book was released on 2019-02-13 with total page 782 pages. Available in PDF, EPUB and Kindle. Book excerpt: This collection gives broad and up-to-date results in the research and development of materials characterization and processing. Topics covered include characterization methods, ferrous materials, non-ferrous materials, minerals, ceramics, polymer and composites, powders, extraction, microstructure, mechanical behavior, processing, corrosion, welding, solidification, magnetic, electronic, environmental, nano-materials, and advanced materials The book explores scientific processes to characterize materials using modern technologies, and focuses on the interrelationships and interdependence among processing, structure, properties, and performance of materials.

Correlative Light and Electron MIcroscopy

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Author : Thomas Muller-Reichert
Publisher : Academic Press
ISBN 13 : 0124160263
Total Pages : 466 pages
Book Rating : 4.1/5 (241 download)

Book Synopsis Correlative Light and Electron MIcroscopy by : Thomas Muller-Reichert

Download or read book Correlative Light and Electron MIcroscopy written by Thomas Muller-Reichert and published by Academic Press. This book was released on 2012-08-10 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods

Springer Handbook of Microscopy

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Author : Peter W. Hawkes
Publisher : Springer Nature
ISBN 13 : 3030000699
Total Pages : 1561 pages
Book Rating : 4.0/5 (3 download)

Book Synopsis Springer Handbook of Microscopy by : Peter W. Hawkes

Download or read book Springer Handbook of Microscopy written by Peter W. Hawkes and published by Springer Nature. This book was released on 2019-11-02 with total page 1561 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book features reviews by leading experts on the methods and applications of modern forms of microscopy. The recent awards of Nobel Prizes awarded for super-resolution optical microscopy and cryo-electron microscopy have demonstrated the rich scientific opportunities for research in novel microscopies. Earlier Nobel Prizes for electron microscopy (the instrument itself and applications to biology), scanning probe microscopy and holography are a reminder of the central role of microscopy in modern science, from the study of nanostructures in materials science, physics and chemistry to structural biology. Separate chapters are devoted to confocal, fluorescent and related novel optical microscopies, coherent diffractive imaging, scanning probe microscopy, transmission electron microscopy in all its modes from aberration corrected and analytical to in-situ and time-resolved, low energy electron microscopy, photoelectron microscopy, cryo-electron microscopy in biology, and also ion microscopy. In addition to serving as an essential reference for researchers and teachers in the fields such as materials science, condensed matter physics, solid-state chemistry, structural biology and the molecular sciences generally, the Springer Handbook of Microscopy is a unified, coherent and pedagogically attractive text for advanced students who need an authoritative yet accessible guide to the science and practice of microscopy.

Cellular Imaging

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Author : Eric Hanssen
Publisher : Springer
ISBN 13 : 3319689975
Total Pages : 339 pages
Book Rating : 4.3/5 (196 download)

Book Synopsis Cellular Imaging by : Eric Hanssen

Download or read book Cellular Imaging written by Eric Hanssen and published by Springer. This book was released on 2017-12-30 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

Three-dimensional Electron Microscopy of Macromolecular Assemblies

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Author : Joachim Frank
Publisher : Oxford University Press
ISBN 13 : 0195150961
Total Pages : 427 pages
Book Rating : 4.1/5 (951 download)

Book Synopsis Three-dimensional Electron Microscopy of Macromolecular Assemblies by : Joachim Frank

Download or read book Three-dimensional Electron Microscopy of Macromolecular Assemblies written by Joachim Frank and published by Oxford University Press. This book was released on 2006 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.

cryoEM

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Author : Tamir Gonen
Publisher : Humana
ISBN 13 : 9781071609651
Total Pages : 350 pages
Book Rating : 4.6/5 (96 download)

Book Synopsis cryoEM by : Tamir Gonen

Download or read book cryoEM written by Tamir Gonen and published by Humana. This book was released on 2020-12-27 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology.

Microscopic Techniques in Biotechnology

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Author : Michael Hoppert
Publisher : John Wiley & Sons
ISBN 13 : 3527605231
Total Pages : 342 pages
Book Rating : 4.5/5 (276 download)

Book Synopsis Microscopic Techniques in Biotechnology by : Michael Hoppert

Download or read book Microscopic Techniques in Biotechnology written by Michael Hoppert and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on all current applications, this book presents the various methods as well as their suitability and limitations for a specific question. One particular highlight is the presentation of all basic information on the structure of the relevant objects, thus allowing readers to choose the most suitable applications for any specific problem. They will also find in-depth background information on structure-function relationships, plus descriptions of sample preparations with respect to a particular technique and the necessary equipment. The whole is rounded off with an overview of the future application potential for devices and applications of upcoming interest in biotechnology.

Scanning Electron Microscopy for the Life Sciences

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Author : Heide Schatten
Publisher : Cambridge University Press
ISBN 13 : 0521195993
Total Pages : 275 pages
Book Rating : 4.5/5 (211 download)

Book Synopsis Scanning Electron Microscopy for the Life Sciences by : Heide Schatten

Download or read book Scanning Electron Microscopy for the Life Sciences written by Heide Schatten and published by Cambridge University Press. This book was released on 2013 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.