Steroid Receptor Methods

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Steroid Receptor Methods

Author : Benjamin A. Lieberman
Publisher : Springer Science & Business Media
ISBN 13 : 0896037541
Total Pages : 377 pages
Book Rating : 4.8/5 (96 download)

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Book Synopsis Steroid Receptor Methods by : Benjamin A. Lieberman

Download or read book Steroid Receptor Methods written by Benjamin A. Lieberman and published by Springer Science & Business Media. This book was released on 2001-08-10 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: A distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA binding characteristics, hormone binding assays, and the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness.

Steroid Receptors

Author : Gabriella Castoria
Publisher :
ISBN 13 : 9781493913466
Total Pages : 245 pages
Book Rating : 4.9/5 (134 download)

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Book Synopsis Steroid Receptors by : Gabriella Castoria

Download or read book Steroid Receptors written by Gabriella Castoria and published by . This book was released on 2014 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Steroid Receptors: Methods and Protocols presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Steroid Receptors: Methods and Protocols offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of steroid receptors in single or different functionally connected cellular contexts under a variety of physiological and pathological conditions, above all tumors.

Localization Of Putative Steroid Receptors

Author : Pertschuk
Publisher : CRC Press
ISBN 13 : 1351082604
Total Pages : 183 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Localization Of Putative Steroid Receptors by : Pertschuk

Download or read book Localization Of Putative Steroid Receptors written by Pertschuk and published by CRC Press. This book was released on 2018-02-01 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: These volumes represent the "state-of-the-art" in morphological methods for detection of steroid receptors. Some physician authors have applied many of the new methodologies in clinical research projects deisgned to improve medical practice, and these attempts have been placed in Volume II. The Background and recent advances presented here are intended to stimulate further experimentation and new innovations, and spur clinical trials by all investigators with an interest in steroid hormones, cell biology, and clinical oncology.

Steroid Receptors in Health and Disease

Author : Virinder Moudgil
Publisher : Springer Science & Business Media
ISBN 13 : 1468455419
Total Pages : 329 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Steroid Receptors in Health and Disease by : Virinder Moudgil

Download or read book Steroid Receptors in Health and Disease written by Virinder Moudgil and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the last two decades, progress in steroid hormone research has resulted in the development of new approaches to contraception as well as diagnosis and treatment of endocrine disorders and cancers. Although significant advances have been made in the purification, characterization, immunochemistry and molecular biology of steroid receptors, the precise molecular mechanism of steroid hormone action has remained obscure. This book captures the detailed presentations made at the first conference on Steroid Receptors in Health and Disease held at Meadow Brook Hall, Oakland University in the fall of 1987. The purpose of this international con ference was to facilitate scientific exchange toward a better understand ing of the mode of action of steroid hormones. The scientific sessions consisted of poster presentations and state-of-the-art lectures, the latter of which make up this volume. The first chapter is meant to provide the reader with a more general background of the topics covered in the book, as well as to discuss certain theme-related issues that are either not yet well-established or accepted or are in the stage of infancy. It is hoped that this volume will serve as a useful treatise for students and investigators interested in basic and clinical aspects of biological regulation by steroid hormones. A task of this magnitude could not have been undertaken without the encouragement, advice and continued generous assistance of the members of the scientific committee. I am gratefully indebted to Drs.

Steroid Hormone Receptor Systems

Author : W. W. Leavitt
Publisher : Springer Science & Business Media
ISBN 13 : 1475765894
Total Pages : 486 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Steroid Hormone Receptor Systems by : W. W. Leavitt

Download or read book Steroid Hormone Receptor Systems written by W. W. Leavitt and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: The papers in this volume were presented at the Symposium on Steroid Hormone Receptor Systems held October 18-20, 1978, at the Worcester Foundation for Experimental Biology, Shrewsbury, Mass. The meeting was organized to review, discuss, and disseminate new knowledge about the regulation and function of the receptor proteins which mediate estrogen, progestin, glucocorticoid, and androgen action. The symposium brought together leading scientists whose interests span the spectrum of biological organization. On this occasion, Drs. Elwood V. Jensen and Etienne E. Baulieu were honored as recipients of the Tenth Annual Gregory Pincus l1emorial Award for their pioneering studies of steroid hormone receptors. The material covered in this book focuses on the molecular mechanisms which control receptor site availability and function. The topics discussed include recent work on receptor antibodies, antiestrogen action, receptor heterogeneity, nuclear binding and processing of receptor, receptor activation and inactivation mechan isms, interactions between receptor systems, influence of biological rhythms, membrane binding sites, and the evolution of steroid-bind ing proteins. Although studies on steroid receptors have led to the development of new approaches for cancer therapy and contraception, much remains to be discovered in this rapidly expanding field. Hope fully, this book will provide added impetus to the quest for a full understanding of steroid receptor systems by drawing attention to the unresolved questions yet to be answered. Hhile the book is intended primarily for those interested in hormone action, it should be of value to a more general audience including cell, molecular, and developmental biologists.

Steroid Hormone Receptors

Author : Colin Roy Clark
Publisher :
ISBN 13 : 9783527264711
Total Pages : 288 pages
Book Rating : 4.2/5 (647 download)

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Book Synopsis Steroid Hormone Receptors by : Colin Roy Clark

Download or read book Steroid Hormone Receptors written by Colin Roy Clark and published by . This book was released on 1987 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Steroid Hormone Receptors: Basic and Clinical Aspects

Author : V. K. Moudgil
Publisher : Springer Science & Business Media
ISBN 13 : 1461598494
Total Pages : 519 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Steroid Hormone Receptors: Basic and Clinical Aspects by : V. K. Moudgil

Download or read book Steroid Hormone Receptors: Basic and Clinical Aspects written by V. K. Moudgil and published by Springer Science & Business Media. This book was released on 2013-03-13 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past few years have witnessed the emergence of steroid hormones as the wonder molecules which generate as much discussion in the scientific literature as they do in a typical living room. This transition has been a result of the tremendous public and scientific interest in the normal functioning of the hor mones as well their suggested involvement in several clinical conditions. In the recent past, notable scientific and technological advances have been made in the areas of contraception and regulation of fertility. Steroid receptors are the indis pensable mediators of hormonal responses and are complex protein molecules which appear to exist in association with other, yet undefined, proteins and/or factors. Receptors for vitamin D, retinoic acid and the thyroid hormones share structural similarities with steroid receptors, and the roster of this superfamily is still expanding. While our knowledge of the diversity and magnitude of steroid effects has advanced, the precise mode of steroid hormone action has alluded investigators. This volume brings together an international team of prominent investigators who discuss their most recent work on the basic and clinical aspects of steroid/nuclear receptors. The contributions represent updated versions of the invited presentations made at The Second Meadow Brook Conference on Steroid Receptors in Health and Disease. I am grateful to my colleagues on the Scientific Committee: Etienne Baulieu, Jack Gorski, Benita Katzenellenbogen, David Toft and James WittJiff, who provided the vision and guidance in formulating an out standing program.

Localization Of Putative Steroid Receptors

Author : Louis P. Pertschuk
Publisher : CRC Press
ISBN 13 : 1351091042
Total Pages : 419 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Localization Of Putative Steroid Receptors by : Louis P. Pertschuk

Download or read book Localization Of Putative Steroid Receptors written by Louis P. Pertschuk and published by CRC Press. This book was released on 2019-07-23 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: These volumes represent the "state-of-the-art" in morphological methods for detection of steroid receptors. In Volume I we have attempted to collect current available methods and experimental approahes which might be useful in solving present enigmas. The background and recent advances presented are intended to stimulate further experimentation and new innovations, and spur clinical trials by all investigators with an interest in steroid hormones, cell biology, and clinical oncology.

Immunocytochemistry for Steroid Receptors

Author : Louis P. Pertschuk
Publisher : CRC Press
ISBN 13 : 9780849369438
Total Pages : 226 pages
Book Rating : 4.3/5 (694 download)

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Book Synopsis Immunocytochemistry for Steroid Receptors by : Louis P. Pertschuk

Download or read book Immunocytochemistry for Steroid Receptors written by Louis P. Pertschuk and published by CRC Press. This book was released on 1990-08-15 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive review of the literature on steroid hormone receptor immuncytochemistry. It includes the development of the first monoclonal antireceptor antibody and initial procedures designed to localize steroid receptors in situ up to the present. The step-by-step instructions provide easy reading of topics such as tissue handling, processing, and interpretation. This text places particular emphasis on the studies of human material-especially mammary carcinoma. It presents specific details with chapters on estrogen and progesterone receptors in breast malignancies and benign breast disease. Each section of this outstanding book contains comprehensive photomicrographs that illustrate the most salient features discussed. Detailed graphs pertaining to theoretical particulars concerning technology and the relationship between image analysis and endocrine response are also included. This monograph is an excellent resource for all researchers concerned with hormone-dependent tissues, pathologists who have an interest in working with novel techniques for localizing steroid hormone receptors, and oncologists who must use assay results in the treatment of breast cancer.

An Introduction to Neuroendocrinology

Author : Richard E. Brown
Publisher : Cambridge University Press
ISBN 13 : 9780521426657
Total Pages : 434 pages
Book Rating : 4.4/5 (266 download)

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Book Synopsis An Introduction to Neuroendocrinology by : Richard E. Brown

Download or read book An Introduction to Neuroendocrinology written by Richard E. Brown and published by Cambridge University Press. This book was released on 1994-01-27 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed as an introductory text in neuroendocrinology; the study of the interaction between the brain and endocrine system and the influence of this on behaviour. The endocrine glands, pituitary gland and hypothalamus and their interactions and hormones are discussed. The action of steroid and thyroid hormone receptors and the regulation of target cell response to hormones is examined. The function of neuropeptides is discussed with respect to the neuroendocrine system and behaviour. The neuroimmune system and lymphokines are described and the interaction between the neuroendocrine and neuroimmune systems discussed. Finally, methods for studying hormonal influences on behaviour are outlined. Each chapter has review and essay questions designed for advanced students and honours or graduate students with a background in neuroscience, respectively.

Neurobiology of Steroids

Author : E. Ronald de Kloet
Publisher : Academic Press
ISBN 13 : 1483288366
Total Pages : 573 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Neurobiology of Steroids by : E. Ronald de Kloet

Download or read book Neurobiology of Steroids written by E. Ronald de Kloet and published by Academic Press. This book was released on 2013-10-22 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: Steroid hormones are unique compounds in that they are active at the interface of peripheral endocrine events and neural mechanisms. Thus their effects present an important peripheral signaling system to alter brain function. This volume presents state-of-the-art and classical techniques for the study of steroid hormones and their receptors and their effects and actions. Comprehensive protocols included for the study of Steroid kinetics and metabolism Steroid receptors Molecular and cellular effects of steroids Steroid effects on integrated systems

Gene Regulation by Steroid Hormones IV

Author : Arun K. Roy
Publisher : Springer Science & Business Media
ISBN 13 : 1461236665
Total Pages : 247 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Gene Regulation by Steroid Hormones IV by : Arun K. Roy

Download or read book Gene Regulation by Steroid Hormones IV written by Arun K. Roy and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first Meadowbrook Symposium was held in 1978 and during the intervening ten years our knowledge concerning how steroid hormones function at the level of gene expression has advanced by leaps and bounds. In this volume, which sum marizes our fourth meeting, these advances are very evident. What seemed like science fiction ten years ago has become commonplace science. Who would have imagined that we could synthesize a nucleotide sequence that binds a specific steroid receptor and acts as a controlling element for gene expression? No one; but as is evident from the results reported in several chapters, this technique is yielding a wealth of information. Using these and other techniques it has become apparent that gene transcription is controlled by interactions between transacting factors and DNA recognition sequences (response elements). These transacting factors appear to be members of a large gene family that includes steroid hormone receptors, transcription factors, protooncogenes and homeobox proteins. Thus a great deal has been learned, but as usual, questions remain. Many of these questions are posed by the findings and observations found in several chapters in this volume. Non hormone binding forms of steroid receptors and their relevance to receptor down regulation, recycling and biological response remain a mystery. The quantitative relationship between receptor binding and biological response still presents agonizing problems. These and many other intriguing questions are discussed in this volume and set the stage for what should be a most rewarding time in endocrinology. Winter 1989 ARUN K.

Steroid Hormones

Author : Brian Green
Publisher :
ISBN 13 :
Total Pages : 284 pages
Book Rating : 4.:/5 (89 download)

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Book Synopsis Steroid Hormones by : Brian Green

Download or read book Steroid Hormones written by Brian Green and published by . This book was released on 1987 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Clinical Interest of Steroid Hormone Receptors in Breast Cancer

Author : G. Leclercq
Publisher : Springer Science & Business Media
ISBN 13 : 364282188X
Total Pages : 381 pages
Book Rating : 4.6/5 (428 download)

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Book Synopsis Clinical Interest of Steroid Hormone Receptors in Breast Cancer by : G. Leclercq

Download or read book Clinical Interest of Steroid Hormone Receptors in Breast Cancer written by G. Leclercq and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several papers dealing with the clinical relevance of steroid hormone receptors in breast cancer have already been published. However, no publication has overviewed studies currently being conducted in Europe, nor is there a register of the european centers performing receptor assays. It has been our purpose to fill these gaps. A large majority of authors who we contacted kindly agreed to con tribute to our book. Papers were grouped into six main parts according to their contents. The abundance of clinical data relevant to receptor assays led us to add critical summaries to help the reader to form his own opinions on the subject. We would like to thank Drs. R. J. B. King, G. Contesso, L. Santi, and E. Engelsman, who helped us in this regard. We hope that this book will enable the reader to become acquainted with the present state of European steroid hormone receptor studies devoted to breast cancer treatment. We also hope that it will promote cooperation between European oncologists involved in this field of research.

Gene Regulation by Steroid Hormones III

Author : Arun K. Roy
Publisher : Springer Science & Business Media
ISBN 13 : 1461246865
Total Pages : 312 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Gene Regulation by Steroid Hormones III by : Arun K. Roy

Download or read book Gene Regulation by Steroid Hormones III written by Arun K. Roy and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of steroid hormone action has continued to expand into the realm of molecular biology at a pace even faster than most of us ever imagined. techniques of molecular biology have made it possible to clone The hormone-regulated genes and to examine steroid-receptor interactions with these genes. Nucleotide sequences of these genes, which show preferential binding of steroid receptors, have been identified. These results are complemented by the identification of chromatin acceptor proteins, which also show preferential binding for steroid-receptor complexes. Thus, one can envision the day when cloned genes, purified acceptor proteins, and receptor-steroid complexes will be recombined in vitro to form a functional unit. Cellular localization of steroid receptors has undergone recent revision, and it now appears that receptors are localized primarily in the nuclear compartment. These findings, although controversial, will lead to a reassessment of many of the previous concepts of steroid-receptor interactions and regulation. The way in which these observations at the of physiology, molecular and cellular levels fit into the overall scheme development, and evolution are continuing to progress, and the future promises some very exciting syntheses of understanding at all levels of biological organization. The third Meadow Brook Conference on hormones was held in order to bring together investigators who will undoubtedly contribute heavily to this future synthesis and to permit a free exchange of ideas and concepts as they relate to the current state-of-the-art in molecular endocrinology. Spring 1986 ARUN K. Roy JAMES H. CLARK Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

The Steroid/Thyroid Hormone Receptor Family and Gene Regulation

Author : J. Carlstedt-Duke
Publisher : Birkhäuser
ISBN 13 : 3034854668
Total Pages : 360 pages
Book Rating : 4.0/5 (348 download)

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Book Synopsis The Steroid/Thyroid Hormone Receptor Family and Gene Regulation by : J. Carlstedt-Duke

Download or read book The Steroid/Thyroid Hormone Receptor Family and Gene Regulation written by J. Carlstedt-Duke and published by Birkhäuser. This book was released on 2013-11-21 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second International CBT (Center for Biotechnology) Synposium was held in Stockholm November 4-5 1988 and had the title "The steroid/thyroid hoITIDne receptor family and gene regulation". This meeting contained contributions from most leading laboratories within the field of steroid/thyroid hOITIDne receptors and gave a very exciting perspective on the dynamic development of this i:ntx>rt. ant research field. Today all known steroid hoITIDne receptors have been cloned and sequenced and novel members of this supergene family are constantly being discovered. Sane of these new receptors remain to be characterized regarding the nature of their ligand. Access to probes for steroid receptors has enabled studies on mechanisms of regulation of receptor gene expression. Deletion and II'Altational analysis of steroid receptor cDNAs followed by expression in cells together with suitable reporter genes has yielded a detailed knowledge about the functional significance of the various domains the receptors are composed of. In certain cases, steroid resistance in patients have been shown to be due to point mutations in the corresponding steroid receptor genes resulting in non-functional receptors. The availability of receptor cDNAs also makes it possible to express receptors at high levels in procaryotic and eucaryotic cells. It is e. g. possible to express the DNA-binding domain of the glucocorticoid receptor in E. coli as a fusion protein with protein A which interacts specifically with DNA. Such studies are necessary for production of sufficient quantities of receptors to allow crystallization and X-ray crystallography for detailed structural information.

Molecular Biology of Steroid and Nuclear Hormone Receptors

Author : Leonard Freedman
Publisher : Springer Science & Business Media
ISBN 13 : 1461217644
Total Pages : 330 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Molecular Biology of Steroid and Nuclear Hormone Receptors by : Leonard Freedman

Download or read book Molecular Biology of Steroid and Nuclear Hormone Receptors written by Leonard Freedman and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intracellular Receptors: New Instruments for a Symphony of Signals In the late eighteenth century, it was proposed on theoretical grounds that each of the body's organs, beginning with the brain, must be "a factory and laboratory of a specific humor which it returns to the blood", and that these circulating signals "are indispensable for the life of the whole" (Bordeu 1775). During the nineteenth cen tury, some remarkable physiological experiments revealed the actions of humoral factors that affected the for and function of multiple tissues, organs and organ sys tems within the body (Berthold 1849); much later, the chemical and molecular na ture of some of those factors was determined. Against this deep historical backdrop of the founding studies of intercellular signaling, molecular biology sprang into existence a mere forty years ago, rooted in the revelation of regulable gene expression in bacteria. But contemporaneous with those classical analyses of transcriptional regulation of the lactose operon, the mod em era of signal transduction was inaugurated by the identification of cAMP as a second messenger -- an intracellular mediator of hormonal activation of glycogen catabolism (Sutherland and RaIl 1960). Later in that same decade, it emerged that cAMP is a critical signal not only in metazoans, but even in bacteria, where it serves an analogous function as a critical switch that activates expression of genes re quired for catabolism of complex carbon sources, including those of the lactose operon.