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Stem Cells Derived From Dental Pulp And Their Application Potential On Tissue Regeneration
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Book Synopsis Stem Cells Derived from Dental Pulp and Their Application Potential on Tissue Regeneration by : Weibo Zhang
Download or read book Stem Cells Derived from Dental Pulp and Their Application Potential on Tissue Regeneration written by Weibo Zhang and published by . This book was released on 2007 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Dental Stem Cells: Regenerative Potential by : Barbara Zavan
Download or read book Dental Stem Cells: Regenerative Potential written by Barbara Zavan and published by Humana Press. This book was released on 2016-07-25 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the basic aspects of dental stem cells (DSCs) as well as their clinical applications in tissue engineering and regenerative medicine. It opens with a discussion of classification, protocols, and properties of DSCs and proceeds to explore DSCs within the contexts of cryopreservation; epigenetics; pulp, periodontal, tooth, bone, and corneal stroma regeneration; neuronal properties, mesenchymal stem cells and biomaterials; and as sources of hepatocytes for liver disease treatment. The fifteen expertly authored chapters comprehensively examine possible applications of DSCs and provide invaluable insights into mechanisms of growth and differentiation. Dental Stem Cells: Regenerative Potential draws from a wealth of international perspectives and is an essential addition to the developing literature on dental stem cells. This installment of Springer’s Stem Cell Biology and Regenerative Medicine series is indispensable for biomedical researchers interested in bioengineering, dentistry, tissue engineering, regenerative medicine, cell biology and oncology.
Book Synopsis Dental Stem Cells by : Fikrettin Şahin
Download or read book Dental Stem Cells written by Fikrettin Şahin and published by Springer. This book was released on 2016-06-08 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores Dental Stem Cell (DSC) biology, from a review of basic concepts for cell culture, to isolation, self-renewal, multipotency and differentiation, regulation by molecular medicine, and prospective research areas for regenerative medicine. The first seven chapters delve into basic DSC properties, vital signaling pathways involved in differentiation, pluripotency, iPS cell development from DSCs, and genetic engineering approaches of DSCs in accordance with the current literature. A comprehensive review of possible clinical applications and in vitro/in vivo studies follows, illustrating the future of DSC research for in the tissue engineering field. The text also discusses the political, ethical, social, and legal ramifications of the use of dental stem cells. Expertly authored and drawing from a multitude of international perspectives, Dental Stem Cells is an invaluable addition to Springer’s Stem Cell Biology and Regenerative Medicine series. It is essential reading for advanced graduate students, basic researchers, and clinical investigators in the fields of stem cell therapy, biological sciences of dentistry, and regenerative medicine.
Book Synopsis Dental Pulp Stem Cells by : Sibel Yildirim
Download or read book Dental Pulp Stem Cells written by Sibel Yildirim and published by Springer Science & Business Media. This book was released on 2012-10-15 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cell technology is moving forward at a tremendous rate. Recent discoveries have surprised even the most expert researchers. While every piece of new data broadens the current knowledge and contributes to this moving forward, the new data also serve as paradigm shifters of fundamental knowledge of cell biology. While the question ‘What is a Stem Cell’ may now seem to basic to even discuss, there are still some discrepancies, however, between groups in terms of their functional roles. Teeth develop from the ectoderm of the first branchial arch and the ectomesenchyme of the neural crest. Deciduous teeth start to form between the sixth and eighth weeks, and permanent teeth begin to form in the twentieth weeks. Several studies have demonstrated that the pulp from both adult teeth and deciduous teeth contains dental pulp stem cells. Several factors have made them very attractive as a model system for many researchers; they are multipotent, ethically and non-controversially available in large numbers, immuno-compatible, developmentally primitive, easy to isolate and have high expansion potential in vitro. However, many controversies still exist in the field. There are several unanswered questions in the biology of dental pulp and odontoblasts. This new volume in the SpringerBriefs in Stem Cells series presents an evaluation of stem cells from human dental pulp as a reliable stem cell source for cell-based therapy to stimulate tissue regeneration.
Book Synopsis Characterization of Dental Pulp Stem Cells and Their Potential Clinical Applications by : Kajohnkiart Janebodin
Download or read book Characterization of Dental Pulp Stem Cells and Their Potential Clinical Applications written by Kajohnkiart Janebodin and published by . This book was released on 2013 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dental pulp stem cells (DPSCs) were first isolated and characterized from human teeth and most studies have focused on using human DPSCs for dentin regeneration. However, mouse DPSCs have not been well characterized and their origin(s) have not yet been elucidated. I examined if murine DPSCs are neural crest derived and determined their in vitro and in vivo capacity. DPSCs from neonatal mice expressed embryonic stem cell and neural crest genes, but lacked expression of mesodermal genes. Cells isolated from the Wnt1-Cre/R26R-LacZ mouse, a reporter of neural crest-derived tissues, indicated that DPSCs were Wnt1-marked and therefore of neural crest origin. Clonal DPSCs showed multi-differentiation in neural crest lineage for odontoblasts, chondrocytes, adipocytes, neurons, and smooth muscles. In vivo subcutaneous transplantation with hydroxyapatite/tricalcium phosphate, based on tissue/cell morphology and specific antibody staining, revealed that the clones differentiated into odontoblast-like cells and produced dentin/pulp-like structure. Conversely, femur-derived bone marrow stromal cells (BMSCs) gave rise to osteoblast-like cells and generated bone-like structure. Interestingly, the capillary distribution in the DPSC transplants showed close proximity to odontoblasts whereas in the BMSC transplants bone condensations were distant to capillaries resembling dentinogenesis in the former vs. osteogenesis in the latter. Loss of functional salivary gland causes patients' moribidities from difficulties in swallowing and speech, as well as oral diseases. Stem cell therapy is considered a potential therapeutic alternative. However, combinatory approaches including not only salivary gland stem cells but also supportive cells and appropriate extracellular matrix are necessary to form a functional salivary gland. Like tooth formation, the development of salivary gland requires epithelium interacting with neural crest-derived mesenchyme. I used the human salivary gland (HSG) cell line as a model to study the effects of DPSCs on salivary gland differentiation. In vitro differentiation on matrigel showed that HSG alone and HSG co-cultured with Wnt1-Cre/R26R-LacZ derived DPSCs (HSG+DPSC) differentiated into acinar-like structures. However, HSG formed more mature (higher expression of LAMP-1 and CD44), larger and increased numbers of acini in HSG+DPSC. Subcutaneous co-transplantation of HSG and DPSCs with hyaluronic acid (HA) hydrogels after 2 weeks was evaluated by Q-RT-PCR, morphology and immunohistology. Compared to HSG transplants which only showed undifferentiated tumor-like cells, HSG+DPSC demonstrated (1) higher expression of murine mesenchymal marker Fgf-7, (2) higher expression of mature human salivary gland differentiation marker alpha-amylase-1 (AMY-1), (3) higher expression of murine endothelial, vWF, neuronal, NF-200, and angiogenic markers, Vegfr-3 and Vegf-c, (4) mucin-secreting acinar- and duct-like structures with abundant blood vessels at the interface with DPSCs, and (5) more mature glandular structures double-positive for salivary gland differentiation markers CD44 and LAMP-1. These results indicate that DPSCs supported and enhanced HSG differentiation into functional salivary gland tissue. In addition, DPSCs have previously demonstrated potential pericyte-like topography and function. However, the mechanisms regulating their pericyte function are still yet to be elucidated. DPSC angiogenic and pericyte function were investigated Tie2-GFP derived dental pulp cells were negative for GFP driven by the endothelial Tie2 transgene, indicating an absence of endothelial cells. Endothelial cells co-cultured with DPSCs formed more mature in vitro tube-like structures as compared to those co-cultured with BMSCs. Many DPSCs were located adjacent to vascular tubes, suggesting a pericyte location and function. In vivo DPSCs subcutaneously transplanted in matrigel (MG) (DPSC-MG) induced more vessel formation than BMSC-MG. DPSCs expressed higher Vegfd, Vegfr3, EphrinB2 levels. Soluble Flt (sFlt), an angiogenic inhibitor that binds VEGF-A, significantly decreased the amount of blood vessels in DPSC-MG, but not in BMSC-MG. sFlt inhibited VEGFR2 and downstream ERK signaling and down-regulated Vegfa, Vegf receptors and EphrinB2 expression in DPSCs. Therefore, DPSC-induced angiogenesis is VEGF-dependent. DPSCs enhance angiogenesis by secreting VEGF-A, -C, -D and forming tight associations with vessels, resembling pericyte-like cells. Taken together, I demonstrate the existence of neural crest-derived DPSCs with differentiation capacity into cranial mesenchymal tissues and other neural crest-derived tissues. I also illustrate the potential of DPSCs as inductive mesenchyme for salivary gland regeneration, repair, and tissue engineering, and provide first insights into the mechanism(s) of DPSC angiogenic capacity and their function as pericytes. DPSCs hold promise as a stem cell source for regenerating neural crest derived tissues, and the trophic and angiogenic properties of DPSCs also highlight this stem cell source useful for tissue regeneration.
Book Synopsis Tissue Engineering and Regeneration in Dentistry by : Rachel J. Waddington
Download or read book Tissue Engineering and Regeneration in Dentistry written by Rachel J. Waddington and published by John Wiley & Sons. This book was released on 2016-11-18 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue Engineering and Regeneration in Dentistry: Current Strategies presents a thorough update on the current advances, methods and understanding in tissue engineering in dentistry. It offers invaluable tools, case studies, and methodologies for undertaking research, including important biological and practical considerations to facilitate successful migration of research from the bench to the clinic. Offers detailed coverage of the basic underlying principles and scientific evidence, and includes protocols to highlight practical applications Written by an internationally renowned team of expert contributors A must-have read for researchers and specialist clinicians in tissue engineering, oral biology, dental materials science, periodontology and oral surgery
Book Synopsis Stem Cell Biology and Tissue Engineering in Dental Sciences by : Ajaykumar Vishwakarma
Download or read book Stem Cell Biology and Tissue Engineering in Dental Sciences written by Ajaykumar Vishwakarma and published by Academic Press. This book was released on 2014-11-05 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem Cell Biology and Tissue Engineering in Dental Sciences bridges the gap left by many tissue engineering and stem cell biology titles to highlight the significance of translational research in this field in the medical sciences. It compiles basic developmental biology with keen focus on cell and matrix biology, stem cells with relevance to tissue engineering biomaterials including nanotechnology and current applications in various disciplines of dental sciences; viz., periodontology, endodontics, oral & craniofacial surgery, dental implantology, orthodontics & dentofacial orthopedics, organ engineering and transplant medicine. In addition, it covers research ethics, laws and industrial pitfalls that are of particular importance for the future production of tissue constructs. Tissue Engineering is an interdisciplinary field of biomedical research, which combines life, engineering and materials sciences, to progress the maintenance, repair and replacement of diseased and damaged tissues. This ever-emerging area of research applies an understanding of normal tissue physiology to develop novel biomaterial, acellular and cell-based technologies for clinical and non-clinical applications. As evident in numerous medical disciplines, tissue engineering strategies are now being increasingly developed and evaluated as potential routine therapies for oral and craniofacial tissue repair and regeneration. - Diligently covers all the aspects related to stem cell biology and tissue engineering in dental sciences: basic science, research, clinical application and commercialization - Provides detailed descriptions of new, modern technologies, fabrication techniques employed in the fields of stem cells, biomaterials and tissue engineering research including details of latest advances in nanotechnology - Includes a description of stem cell biology with details focused on oral and craniofacial stem cells and their potential research application throughout medicine - Print book is available and black and white, and the ebook is in full color
Book Synopsis Regenerative Dentistry by : Mona Marei
Download or read book Regenerative Dentistry written by Mona Marei and published by Springer Nature. This book was released on 2022-06-01 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dental caries, periodontitis, tooth loss, and bone resorption are considered prevalent health problems that have direct affect on the quality of life. While, advances in stem cell biology and biotechnology have sparked hope for devastating maladies, such as diabetes, cardiovascular diseases, etc., it also provides a strategy of regenerative therapy for dental tissues. From the prospective of tissue engineering, it is of utmost importance to understand and emulate the complex cell interactions that make up a tissue or organ. Unlike other tissues in the body, dental tissues are unique in their development, function, and even in their maintenance throughout life. The harmonized stimulations of biology and mechanical regulators to promote cellular activities have matured our understanding of the value of regenerative therapy of dental tissue versus the reparative treatment. In this book, we review the current knowledge available to regenerate alveolar bone, periodontal structure, and pulp/dentin complex. The book provides researchers with detailed information about development and functional characteristics of the dental unit with detailed protocols covering a comprehensive range of various approaches to engineer dental tissues: to use isolated cells or cell substitutes as cellular replacement, to use acellular biomaterials capable of inducing tissue regeneration, and/or to use a combination of cells, biomaterial and growth factors. We are well aware, with the concept changes in the field toward in-vitro biomimetics of in-vivo tissue development. The theoretical frame work integrating these concepts of developmental biology and developmental engineering is yet to be emphasized and implemented. Until this happens, we consider this book of regenerative dentistry as a call for scientists to achieve, researchers to innovate, practitioners to apply, and students to learn the art and science of regenerative therapy in dentistry. Table of Contents: Introduction to Regenerative Dentistry / Tissue Engineering Alveolar Bone / Tissue Engineering of the Periodontal Tissues / Dynamics for Pulp-Dentin Tissue Engineering in Operative Dentistry
Book Synopsis Dental and Periodontal Tissues Formation and Regeneration: Current Approaches and Future Challenges by : Giovanna Orsini
Download or read book Dental and Periodontal Tissues Formation and Regeneration: Current Approaches and Future Challenges written by Giovanna Orsini and published by Frontiers Media SA. This book was released on 2016-10-07 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sequential and reciprocal interactions between oral epithelial and cranial neural crest-derived mesenchymal cells give rise to the teeth and periodontium. Teeth are vital organs containing a rich number of blood vessels and nerve fibers within the dental pulp and periodontium. Teeth are composed by unique and specific collagenous (dentin, fibrillar cementum) and non-collagenous (enamel) highly mineralized extracellular matrices. Alveolar bone is another collagenous hard tissue that supports tooth stability and function through its close interaction with the periodontal ligament. Dental hard tissues are often damaged after infection or traumatic injuries that lead to the partial or complete destruction of the functional dental and supportive tissues. Well-established protocols are routinely used in dental clinics for the restoration or replacement of the damaged tooth and alveolar bone areas. Recent progress in the fields of cell biology, tissue engineering, and nanotechnology offers promising opportunities to repair damaged or missing dental tissues. Indeed, pulp and periodontal tissue regeneration is progressing rapidly with the application of stem cells, biodegradable scaffolds, and growth factors. Furthermore, methods that enable partial dental hard tissue repair and regeneration are being evaluated with variable degrees of success. However, these cell-based therapies are still incipient and many issues need to be addressed before any clinical application. The understanding of tooth and periodontal tissues formation would be beneficial for improving regenerative attempts in dental clinics. In the present e-book we have covered the various aspects dealing with dental and periodontal tissues physiology and regeneration in 6 chapters: 1. General principles on the use of stem cells for regenerating craniofacial and dental tissues 2. The roles of nerves, vessels and stem cell niches in tissue regeneration 3. Dental pulp regeneration and mechanisms of various odontoblast functions 4. Dental root and periodontal physiology, pathology and regeneration 5. Physiology and regeneration of the bone using various scaffolds and stem cell populations 6. Physiology, pathology and regeneration of enamel using dental epithelial stem cells
Book Synopsis Biomaterials Science and Engineering by : Rosario Pignatello
Download or read book Biomaterials Science and Engineering written by Rosario Pignatello and published by BoD – Books on Demand. This book was released on 2011-09-15 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: These contribution books collect reviews and original articles from eminent experts working in the interdisciplinary arena of biomaterial development and use. From their direct and recent experience, the readers can achieve a wide vision on the new and ongoing potentials of different synthetic and engineered biomaterials. Contributions were not selected based on a direct market or clinical interest, than on results coming from very fundamental studies which have been mainly gathered for this book. This fact will also allow to gain a more general view of what and how the various biomaterials can do and work for, along with the methodologies necessary to design, develop and characterize them, without the restrictions necessarily imposed by industrial or profit concerns. The book collects 22 chapters related to recent researches on new materials, particularly dealing with their potential and different applications in biomedicine and clinics: from tissue engineering to polymeric scaffolds, from bone mimetic products to prostheses, up to strategies to manage their interaction with living cells.
Book Synopsis Stem Cells in Dentistry by : Alisina Karimi
Download or read book Stem Cells in Dentistry written by Alisina Karimi and published by Book Rivers. This book was released on 2022-02-02 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological solutions to biological problems are emerging as a new paradigm in dentistry and medicine. Diagnosis, treatment, therapeutics and biomaterials are all becoming biological and gene-based. We are on the verge of shifting or evolving from mechanical (e.g. surgical) to biological solutions for health promotion, risk assessment, diagnostics, treatments, therapeutics, and health care outcomes.(
Book Synopsis Commitment and Potential of Dental Pulp Stem Cells by : Chi Lee
Download or read book Commitment and Potential of Dental Pulp Stem Cells written by Chi Lee and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Dental pulp stem cells (DPSCs) play an integral role during dentine injury where they migrate towards the injury site, proliferate, differentiate into odontoblast-like cells and secrete a mineralised matrix, protecting the vital pulp tissue and preserving the tooth organ. Dentine matrix proteins (DMP) may have a role in stimulating this reparative dentine formation. Multiple adult stem cells make up the DPSC population. Within this study progenitor cells were isolated from dental pulp by preferential fibronectin adherence (FNA) or using magnetic activated cell sorting (MACS) to isolate P75 expressing neural crest cells. Clonally expanded colonies were established. The proliferative FNA and P75-sorted populations were shown to be distinct progenitors with differing morphology and stem cell marker expression. One FNA clonal population was differentiated into mesenchymal lineages that established it as a multipotent DPSC population. Clonal populations were supplemented with DMP in vitro to examine the potential role of DMP in modulating cell behaviour during dentine injury. Supplementing with DMP had a dose dependent response on DPSC viability, increased cell numbers,reduced apoptosis and promoted cell migration, suggesting that the growth factors in DMP may have a positive synergistic effect on DPSC behaviour. Fluorescently stained DPSCs were microinjected into transverse tooth slices and placed in culture to develop an ex vivo model to study DPSC behaviour in a tooth environment. Injected cells remained viable after 7 days of culture, providing proof of concept data that DPSCs can be localised for study in situ. This thesis was successful in the isolation of clonal populations representing progenitor cells with different characteristics. Supplementing DPSCs with DMP promoted cell behaviour facilitating reparative dentinogenesis, indicating a potential clinical application for DMP in restorative therapy. The study of DPSCs using the ex vivo model will be important in further development of these novel therapies for dental tissue regeneration.
Book Synopsis Handbook of Stem Cell Applications by : Khawaja H. Haider
Download or read book Handbook of Stem Cell Applications written by Khawaja H. Haider and published by Springer Nature. This book was released on with total page 1422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mesenchymal Stem Cells by : Phuc Van Pham
Download or read book Mesenchymal Stem Cells written by Phuc Van Pham and published by BoD – Books on Demand. This book was released on 2017-11-29 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesenchymal Stem Cells: Isolation, Characterization, and Applications thoroughly presents the isolation, characterization, and some applications of mesenchymal stem cells in the clinic. The book has two parts: "Isolation and Characterization" and "Clinical Perspectives and Applications." In Part I, the subsequent chapters introduce some techniques in isolation, characterization, and purification of mesenchymal stem cells in different tissues. In Part II, some applications of mesenchymal stem cells in the popular diseases, which include cartilage regeneration, spinal cord injury, and osteoarthritis, are discussed. This book provides a succinct yet comprehensive overview of mesenchymal stem cells for advanced students, graduate students, and researchers.
Book Synopsis Mesenchymal Stem Cells as New Candidates for Stemcell Based Dental Therapies by : Patrick Kimuyu
Download or read book Mesenchymal Stem Cells as New Candidates for Stemcell Based Dental Therapies written by Patrick Kimuyu and published by GRIN Verlag. This book was released on 2018-02-07 with total page 20 pages. Available in PDF, EPUB and Kindle. Book excerpt: Project Report from the year 2018 in the subject Medicine - Medical Frontiers and Special Areas, grade: 1, Egerton University, language: English, abstract: This paper will provide a comprehensive review on the origin and types of MSCs in the dental tissue and the oral cavity. It will also discuss their therapeutic mechanisms that make them useful in dentistry and dental treatments. Over the past few decades, stem cell research has gained extensive scientific inquiry. This aspect is attributable to the significance of stem cells in tissue engineering. It is apparent that tissue regeneration has emerged as a reliable medical approach for the treatment of tissue disorders and injuries. Initially, embryonic stem cells were preferred as candidates for regenerative medicine because these cells can be induced to replicate in a pluripotent state. However, stem cell research involving embryonic stem cells has attracted immense controversy. It is also associated with legal and ethical issues, thus limiting the use of embryonic stem cells in regenerative medicine. Fortunately, the discovery of mesenchymal stem cells (MSCs), also referred to as adult stem cells, has restored promise for the development of stem cell therapies. Unlike embryonic stem cells, MSCs are free from legal and ethical concerns. MSCs are usually pluripotent progenitor cells that are generated in an array of tissues in both foetal and adult life. It is reported that these progenitor cells differentiate into cell types of the tissues that generate them, although studies indicate that they can differentiate cell types of other tissues. Currently, MSCs are used for regenerative therapies for a number of tissue disorders and injuries including bone regeneration. For instance, MSCs generated by the dental pulps and the oral cavity tissues have been found to possess the potential for dental tissue regeneration. These cells have also been found to useful in non-dental tissue repair.
Book Synopsis Stem Cells in Oral Cavity: From Development to Regeneration by : Mikihito Kajiya
Download or read book Stem Cells in Oral Cavity: From Development to Regeneration written by Mikihito Kajiya and published by Frontiers Media SA. This book was released on 2022-02-22 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Cell Biology and Translational Medicine, Volume 10 by : Kursad Turksen
Download or read book Cell Biology and Translational Medicine, Volume 10 written by Kursad Turksen and published by Springer Nature. This book was released on 2020-10-31 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Much research has focused on the basic cellular and molecular biological aspects of stem cells. Much of this research has been fueled by their potential for use in regenerative medicine applications, which has in turn spurred growing numbers of translational and clinical studies. However, more work is needed if the potential is to be realized for improvement of the lives and well-being of patients with numerous diseases and conditions. This book series 'Cell Biology and Translational Medicine (CBTMED)' as part of SpringerNature’s longstanding and very successful Advances in Experimental Medicine and Biology book series, has the goal to accelerate advances by timely information exchange. Emerging areas of regenerative medicine and translational aspects of stem cells are covered in each volume. Outstanding researchers are recruited to highlight developments and remaining challenges in both the basic research and clinical arenas. This current book is the tenth volume of a continuing series.
